Gene

KWMTBOMO12726  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA007187

Annotation

PREDICTED:_omega-amidase_NIT2_isoform_X1_[Papilio_polytes]

Location

Bomo_Chr21(+):13456650-13461509      View in EpiBrowser

Full name

Omega-amidase NIT2       + More
Omega-amidase NIT2-B      

Alternative Name

Nitrilase homolog 2

Location in the cell

Cytoplasmic   Reliability : 3.503

CDS

ATGTTTGATAATGCGGACTCGGTGCATCCAAGATTCTTAGTAAAGCCCAAATTTAAAATAGGTTTCAAGATAGCATTAATTCAACTATCGGTGGGTCCCGATAAATCTAAGAATATAGCGCAGGCTGTGAAGGAAATCCATTTAGCTAAAGAGAAAGGGGCTCAATTGGTGGCCTTGCCGGAGTGCTTCAACTCTCCTTACGGAACCAAGTATTTTGATGAATATGCAGAGGAAGTGCCATCGGGGGAGACGAGCAGAGCTCTATCCAAAGCCGCTGCAGAAGCTGGAGTGTGCGTAGTTGGAGGTACCGTCCCGGAGAGATACGAGAAAAAATTGTACAACACTTGCACTGTTTGGGATGATACTGGGAAACTCTTAGCGCAACACAGAAAGATGCATCTCTTCGACATAGACATTCCTAACAAGATAACGTTCAAAGAATCCGAGGTTCTCTCTGCGGGCGATAAGATCACATCGTTCGACTTTTTGGGATCAAAGATTGGCATCGGCATATGTTACGATCTGAGGTTCCCTGAGATGGCACATCTGATGGCCAAGGAAGGTTGTTCTCTCCTCATCTATCCGGGGGCGTTCAACATGACGACCGGTCCCCGGCATTGGGAGCTGCTGGGGAGGGCGCGTGCTACCGACCAGCAGCTGTGGGTGGCTCTCGTGAGCCCCGCCCGGGACAGCGCCGCGGGCTACGTCGCCTGGGGACACTCGCTGCTCGTCGACCCCTGGGGACAAGTGGTGGAACAGCTCGACCACGAGCCGGGAGTCTTACTCGCTGATGTTGATCTGAAAGTCGTGGATGAAGTAAGGAGTCAGATACCGATCAGGAAACAGCGCAGAACTGACATCTACGACATCACGATCAGGTCTTAA

Protein

MFDNADSVHPRFLVKPKFKIGFKIALIQLSVGPDKSKNIAQAVKEIHLAKEKGAQLVALPECFNSPYGTKYFDEYAEEVPSGETSRALSKAAAEAGVCVVGGTVPERYEKKLYNTCTVWDDTGKLLAQHRKMHLFDIDIPNKITFKESEVLSAGDKITSFDFLGSKIGIGICYDLRFPEMAHLMAKEGCSLLIYPGAFNMTTGPRHWELLGRARATDQQLWVALVSPARDSAAGYVAWGHSLLVDPWGQVVEQLDHEPGVLLADVDLKVVDEVRSQIPIRKQRRTDIYDITIRS

Description

Has a omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting alpha-ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate and oxaloacetate, respectively.

Catalytic Activity

a monoamide of a dicarboxylate + H2O = a dicarboxylate + NH4(+)

Biophysicochemical Properties

0.0054 mM for alpha-ketoglutaramate (open-chain form)

Subunit

Homodimer.

Similarity

Belongs to the carbon-nitrogen hydrolase superfamily. NIT1/NIT2 family.

Keywords

Complete proteome   Cytoplasm   Hydrolase   Reference proteome   Acetylation   Phosphoprotein  

Feature

chain  Omega-amidase NIT2

Uniprot

H9JCE2   A0A212FCN8   A0A437BE16   A0A194QGE9   A0A2A4JVB6   A0A2H1VP76   + More
A0A2A4JWA6   A0A2W1BU17   A0A1J1HY92   A0A182H6V7   V5I740   A0A067RDQ3   A0A1S4EV34   Q7QKM7   E9IDA5   A0A1I8JTR3   U5EWS5   Q17Q52   A0A195CPT1   A0A182V6Y0   A0A182ULJ6   Q7PQH4   A0A182KXA8   A0A2J7RME0   D6WTV5   A0A2J7RME6   A0A023ENL8   A0A182WVZ1   A0A182J7B0   A0A1S3JZ52   A0A182VS40   A0A1Y2HQE4   A0A084W1R3   W5J2P9   A0A1Q3G486   A0A195D9S4   K7J460   A0A411G625   A0A158NUY5   A0A210PVC6   A0A0A9X6T8   A0A182QAC8   A0A146LEL6   A0A2M4BWG2   A0A182M6S9   A0A2M4BWJ4   A0A182MY10   A0A0D2WUJ5   A0A023F7N2   A0A224XVE0   A0A0B7BE83   A0A1E1XRI4   A0A226DLD4   E2C501   A0A088A404   A0A0P4VNQ6   F4WXK4   A0A3L8DCZ4   E9HCU7   R7TU67   A0A069DYE6   A0A0V0G7F0   Q28IE5   R4G3C0   A0A2S2QXL0   B5DET5   A0A1B6IMY0   F7AD06   A0A2S2PEK3   A7T002   V4A879   A0A182FL18   A0A2L2YDI6   A0A154NVZ0   A0A397IRB0   A0A182JYD2   A0A182YLL0   A0A094KQE6   Q497B0   A0A3M0KFI4   A0A0L7R5Y6   A0A182R8A0   A0A1E1X5M6   A0A1L8HCV8   Q6INI7   A0A0P6ESE2   A0A218UC76   A0A2C9JEI5   A0A2C9JEJ0   A0A0P5A7S0   A0A0P4YJC2   A0A0N8EQV9   A0A131XG22   A0A1B6LV30   A0A091EKS8   A0A423SB03   A0A2Z6QE33   A0A3L8S5T5   U3JTN4   A0A1S4EGF0  

EC Number

3.5.1.3

Pubmed

19121390    22118469    26354079    28756777    26483478    24845553    + More
9087549    12364791    14747013    17210077    21282665    17510324    20966253    18362917    19820115    24945155    24438588    20920257    23761445    20075255    21347285    28812685    25401762    26823975    25474469    29209593    20798317    27129103    21719571    30249741    21292972    23254933    26334808    20431018    17615350    26561354    25244985    15489334    19464248    19595734    28503490    27762356    15562597    28049606    30282656   

EMBL

BABH01031543    AGBW02009156    OWR51504.1    RSAL01000080    RVE48653.1    KQ458981    + More
KPJ04494.1    NWSH01000508    PCG75977.1    ODYU01003620    SOQ42608.1    PCG75978.1    KZ150005    PZC75233.1    CVRI01000020    CRK91105.1    JXUM01115001    KQ565839    KXJ70610.1    GALX01007100    JAB61366.1    KK852701    KDR18133.1    AAAB01008773    EAA03516.3    GL762454    EFZ21354.1    APCN01005482    GANO01001285    JAB58586.1    CH477187    EAT48864.1    KQ977444    KYN02748.1    AAAB01008888    EAA08837.5    NEVH01002562    PNF42007.1    KQ971352    EFA07337.1    PNF42006.1    GAPW01003078    JAC10520.1    MCFL01000015    ORZ36825.1    ATLV01019415    KE525269    KFB44157.1    ADMH02002130    ETN58557.1    GFDL01000441    JAV34604.1    KQ981082    KYN09617.1    AAZX01013336    MH365519    QBB01328.1    ADTU01026824    ADTU01026825    ADTU01026826    NEDP02005466    OWF40424.1    GBHO01027920    GBHO01027919    GBHO01027918    GBRD01001629    GBRD01001628    GBRD01001627    GBRD01001626    GDHC01011614    JAG15684.1    JAG15685.1    JAG15686.1    JAG64192.1    JAQ07015.1    AXCN02001643    GDHC01012580    GDHC01003520    JAQ06049.1    JAQ15109.1    GGFJ01008232    MBW57373.1    AXCM01000846    GGFJ01008231    MBW57372.1    KE346369    KJE95623.1    GBBI01001457    JAC17255.1    GFTR01004525    JAW11901.1    HACG01044438    CEK91303.1    GFAA01001504    JAU01931.1    LNIX01000017    OXA45477.1    GL452712    EFN76982.1    GDKW01002012    JAI54583.1    GL888425    EGI61074.1    QOIP01000010    RLU17759.1    GL732621    EFX70459.1    AMQN01002216    KB308638    ELT97214.1    GBGD01002440    JAC86449.1    GECL01002261    JAP03863.1    CR760444    BC091101    ACPB03005784    GAHY01001816    JAA75694.1    GGMS01012659    MBY81862.1    BC168796    AAI68796.1    GECU01019433    GECU01006824    JAS88273.1    JAT00883.1    AAMC01097136    GGMR01015270    MBY27889.1    DS469991    EDO30709.1    KB202591    ESO89486.1    IAAA01012001    LAA06204.1    KQ434772    KZC03855.1    PQFF01000201    RHZ75250.1    KL260745    KFZ59567.1    BC100637    QRBI01000107    RMC11906.1    KQ414648    KOC66302.1    GFAC01004626    JAT94562.1    CM004468    OCT93942.1    BC072293    GDIP01131226    GDIQ01058575    JAL72488.1    JAN36162.1    MUZQ01000468    OWK51070.1    GDIP01202901    JAJ20501.1    GDIP01226651    JAI96750.1    GDIQ01002796    JAN91941.1    GEFH01003920    JAP64661.1    GEBQ01012445    JAT27532.1    KK718610    KFO57656.1    QCYY01004195    ROT61398.1    BEXD01000557    GBB88453.1    QUSF01000054    RLV97616.1    AGTO01021879   

Proteomes

UP000005204    UP000007151    UP000283053    UP000053268    UP000218220    UP000183832    + More
UP000069940    UP000249989    UP000027135    UP000007062    UP000075840    UP000008820    UP000078542    UP000075903    UP000075902    UP000075882    UP000235965    UP000007266    UP000076407    UP000075880    UP000085678    UP000075920    UP000193411    UP000030765    UP000000673    UP000078492    UP000002358    UP000005205    UP000242188    UP000075886    UP000075883    UP000075884    UP000008743    UP000198287    UP000008237    UP000005203    UP000007755    UP000279307    UP000000305    UP000014760    UP000008143    UP000015103    UP000001593    UP000030746    UP000069272    UP000076502    UP000266861    UP000075881    UP000076408    UP000002494    UP000269221    UP000053825    UP000075900    UP000186698    UP000197619    UP000076420    UP000052976    UP000283509    UP000247702    UP000276834    UP000016665    UP000079169   

PRIDE

H9JCE2    Q28IE5    Q497B0    Q6INI7   

Pfam

PF00795   CN_hydrolase        + More
PF12656   G-patch_2

Interpro

IPR036526   C-N_Hydrolase_sf        + More
IPR003010   C-N_Hydrolase       
IPR001110   UPF0012_CS       
IPR026822   Spp2/MOS2_G-patch       
IPR000467   G_patch_dom       
IPR041993   GPKOW_KOW1       

SUPFAM

SSF56317   SSF56317       

Gene 3D

3.60.110.10    

CDD

cd13152  KOW_GPKOW_A

ProteinModelPortal

H9JCE2   A0A212FCN8   A0A437BE16   A0A194QGE9   A0A2A4JVB6   A0A2H1VP76   + More
A0A2A4JWA6   A0A2W1BU17   A0A1J1HY92   A0A182H6V7   V5I740   A0A067RDQ3   A0A1S4EV34   Q7QKM7   E9IDA5   A0A1I8JTR3   U5EWS5   Q17Q52   A0A195CPT1   A0A182V6Y0   A0A182ULJ6   Q7PQH4   A0A182KXA8   A0A2J7RME0   D6WTV5   A0A2J7RME6   A0A023ENL8   A0A182WVZ1   A0A182J7B0   A0A1S3JZ52   A0A182VS40   A0A1Y2HQE4   A0A084W1R3   W5J2P9   A0A1Q3G486   A0A195D9S4   K7J460   A0A411G625   A0A158NUY5   A0A210PVC6   A0A0A9X6T8   A0A182QAC8   A0A146LEL6   A0A2M4BWG2   A0A182M6S9   A0A2M4BWJ4   A0A182MY10   A0A0D2WUJ5   A0A023F7N2   A0A224XVE0   A0A0B7BE83   A0A1E1XRI4   A0A226DLD4   E2C501   A0A088A404   A0A0P4VNQ6   F4WXK4   A0A3L8DCZ4   E9HCU7   R7TU67   A0A069DYE6   A0A0V0G7F0   Q28IE5   R4G3C0   A0A2S2QXL0   B5DET5   A0A1B6IMY0   F7AD06   A0A2S2PEK3   A7T002   V4A879   A0A182FL18   A0A2L2YDI6   A0A154NVZ0   A0A397IRB0   A0A182JYD2   A0A182YLL0   A0A094KQE6   Q497B0   A0A3M0KFI4   A0A0L7R5Y6   A0A182R8A0   A0A1E1X5M6   A0A1L8HCV8   Q6INI7   A0A0P6ESE2   A0A218UC76   A0A2C9JEI5   A0A2C9JEJ0   A0A0P5A7S0   A0A0P4YJC2   A0A0N8EQV9   A0A131XG22   A0A1B6LV30   A0A091EKS8   A0A423SB03   A0A2Z6QE33   A0A3L8S5T5   U3JTN4   A0A1S4EGF0  

PDB

2W1V     E-value=1.18606e-80,     Score=762

KEGG

101736797    K13566 omega-amidase [EC:3.5.1.3] | (RefSeq) omega-amidase NIT2

PATHWAY

00250    Alanine, aspartate and glutamate metabolism - Bombyx mori (domestic silkworm)
01100    Metabolic pathways - Bombyx mori (domestic silkworm)

GO

GO:0006807   GO:0016021   GO:0050152   GO:0006107   GO:0006541   GO:0006528   GO:0016787   GO:0003676   GO:0005737   GO:0005829   GO:0005813   GO:0016810   GO:0006281   GO:0016020   GO:0009156   GO:0044249   GO:0003677   GO:0004879   GO:0003707   GO:0016286  

Subcellular location

Cytoplasm  

Length:

294

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.01269

Exp number, first 60 AAs:

0.00661

Total prob of N-in:

0.01902

outside

1  -  294

 

 

Pi

23.599085

Theta

22.970155

Tajima's D

0.133075

CLR

0.942582

CSRT

0.4026798660067

Interpretation

Uncertain