Gene

KWMTBOMO12683  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA001396

Annotation

PREDICTED:_very-long-chain_(3R)-3-hydroxyacyl-CoA_dehydratase_[Papilio_polytes]

Location

Bomo_Chr21(-):11491141-11495732      View in EpiBrowser

Full name

Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase      

Location in the cell

PlasmaMembrane   Reliability : 3.527

CDS

ATGGTTAAACCGAGTCCATTTGTGTATTGGGCTCAAACAGAAAGTACTATTTCACTGAAAATTGATTTGAAAAATGCATTAAAACCTGATGTGAAAGTTCTCGATAATAATGTAATATTTACCGCAAAGGGCACAGGAGCCCGGGGTGAGAGTCAATATGAGTTTAATCTGGATTTATTTTCAAATATTAAAAGTCTTGAGCCCGGTGGAGAACATATCACACTTCGAGTGTTCGAAAACAGAATCGAGCTGATCTTGCAAAAGGTGAAACCTGTATGGTGGCCTCGTCTCACAGCACAGCCACAAAAACCTGCCTGGTTGAAGATAAATTTTGATTTGTGGAAATCTGAAGACGGCCAGGAAAGTGACGAAGAGAAGCGTGACGTAATGAGAGACTACCCAGGAATGTATGATCAGCTACACCAAGAAGAAATGGGATATAGGAAAGAAGACCTCAAGAAAGTTTACCTGTTTATCTACAATCTTTTCCAATTTGTTGGATACATGTATGTAATGGCGGTGATGGGAGTGCGTTATGTCAAACTTGATTATGATTCTGTGACTGATACATACGAACATGTTGGTTCGGCCATGAAATTTTTACAGTTAATGCAATATCTTGAAGTGCTACATCCTATGTTTGGTTATACGAAGGGTGGAGTTCTAGTACCATTCCTACAAGTTACGGGCAGAGCCTTTGTACTGTTTATAATGATAGATGCTGAACCAAGAATGCAGACAAAACCAGTTGTCTTCTATCTATTTGTTGTGTGGACATGCATTGAAATTGTTAGATACCCTTACTACATGTCCCAGTTGTACAAGAAGGAATTTGCCTTGTTAACATGGCTGCGATACACATTGTGGATACCGCTTTACCCAATGGGTATAGTATGTGAAGCCACAATAATACTCCGCAACATACCATACTTTGAAGAGACTCAGAAATACACAGTATCGCTGCCAAACGCCTGGAACTTCGCCTTCCACATGCCAACTTTCTTAAAATTTTATTTATTAGTCCTAACATTCCCCGGTATGTTTCTAGTAATGAAACATATGCATCGGCTTAGAAATATCAAACTTAGACCGAAAATTGTCATTAAGAAATCTAATTAG

Protein

MVKPSPFVYWAQTESTISLKIDLKNALKPDVKVLDNNVIFTAKGTGARGESQYEFNLDLFSNIKSLEPGGEHITLRVFENRIELILQKVKPVWWPRLTAQPQKPAWLKINFDLWKSEDGQESDEEKRDVMRDYPGMYDQLHQEEMGYRKEDLKKVYLFIYNLFQFVGYMYVMAVMGVRYVKLDYDSVTDTYEHVGSAMKFLQLMQYLEVLHPMFGYTKGGVLVPFLQVTGRAFVLFIMIDAEPRMQTKPVVFYLFVVWTCIEIVRYPYYMSQLYKKEFALLTWLRYTLWIPLYPMGIVCEATIILRNIPYFEETQKYTVSLPNAWNFAFHMPTFLKFYLLVLTFPGMFLVMKHMHRLRNIKLRPKIVIKKSN

Description

Catalyzes the third of the four reactions of the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. This enzyme catalyzes the dehydration of the 3-hydroxyacyl-CoA intermediate into trans-2,3-enoyl-CoA, within each cycle of fatty acid elongation. Thereby, it participates to the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators.

Catalytic Activity

a very-long-chain (3R)-hydroxyacyl-CoA = a very-long-chain (2E)-enoyl-CoA + H2O

Similarity

Belongs to the very long-chain fatty acids dehydratase HACD family.

Feature

chain  Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase

Uniprot

H9IVW5   A0A1E1WHM1   A0A194QDQ7   A0A2A4JQM1   A0A212F7A3   A0A3S2P7D4   + More
A0A0N0U7H8   A0A067QZZ2   A0A2A3EBK3   U5ENU0   A0A0L7R4H6   A0A088AFL9   A0A1J1IPI1   R4G8F4   A0A069DYT1   E2AFV5   F4WRF0   A0A158NUK9   A0A023F8E3   A0A151X6F6   A0A0V0G852   A0A224XSD5   A0A151JQ82   A0A195FJN5   A0A195BF81   A0A2J7R263   A0A151IFW3   A0A0L0BKW8   B4P373   B4IEM1   B4Q4U1   A0A026W5T6   Q9VJX8   A0A1A9Z9E1   W8BUI6   B3NAW1   A0A1A9VZR8   Q8SYI0   E0VMB9   B4M8H6   A0A1A9Y4Z3   A0A336MJD7   A0A1A9UYA3   A0A336K3R8   A0A1B0AWX9   A0A1B6GWX8   E2BIT0   A0A1I8MJA2   A0A1B6C2M9   Q29PA2   B4GKD3   A0A3B0KKX3   A0A1I8PN46   A0A0L7LGE2   A0A1W4UVF4   A0A1B6DZR9   B4MWF3   A0A1B6KK69   A0A2R7WV92   B3MVB4   B4KIF8   D2A528   A0A154P9N8   A0A0M3QTU3   B4JCT9   A0A0A9YMM9   A0A0K8TC72   K7J872   A0A0P4WFV7   A0A1W4WT73   A0A0P4WP27   A0A3R7PUF3   A0A0P5BWV1   A0A0N8BLU7   E9HPF0   A0A0P5M4B4   A0A0P5UDY0   A0A0P4ZSN7   A0A0P6DH87   A0A3B0JZE8   A0A0N8DBU2   A0A0P5YTF5   A0A0P5ZCI3   A0A0P6AKU7   A0A0P6JUY3   A0A0N8D2V4   A0A1B6HU10   A0A0C9R5Y1   A0A0P5MZ34   A0A0N7ZQE9   A0A2R5LAJ8   A0A1D2NGU6   A0A0P5SLZ6   A0A1V9XX68   A0A0P5YMP8   A0A1L8DYX2   A0A023FX35   G3MKT5  

EC Number

4.2.1.134

Pubmed

19121390    26354079    22118469    24845553    26334808    20798317    + More
21719571    21347285    25474469    26108605    17994087    17550304    22936249    24508170    30249741    10731132    12537568    12537572    12537573    12537574    16110336    17569856    17569867    26109357    26109356    24495485    20566863    18057021    25315136    15632085    26227816    18362917    19820115    25401762    26823975    20075255    21292972    27289101    28327890    22216098   

EMBL

BABH01006543    GDQN01005975    GDQN01004564    JAT85079.1    JAT86490.1    KQ459193    + More
KPJ03100.1    NWSH01000885    PCG73710.1    AGBW02009897    OWR49615.1    RSAL01000006    RVE54246.1    KQ435710    KOX79701.1    KK852805    KDR16121.1    KZ288301    PBC28844.1    GANO01000451    JAB59420.1    KQ414657    KOC65785.1    CVRI01000057    CRL02072.1    ACPB03003866    GAHY01001067    JAA76443.1    GBGD01001850    JAC87039.1    GL439158    EFN67704.1    GL888285    EGI63221.1    ADTU01026592    GBBI01001101    JAC17611.1    KQ982481    KYQ55985.1    GECL01002192    JAP03932.1    GFTR01005537    JAW10889.1    KQ978660    KYN29394.1    KQ981522    KYN40613.1    KQ976504    KYM82869.1    NEVH01008200    PNF34928.1    KQ977771    KYN00031.1    JRES01001704    KNC20740.1    CM000157    EDW88315.1    CH480831    EDW46048.1    CM000361    CM002910    EDX04929.1    KMY90111.1    KK107390    QOIP01000007    EZA51437.1    RLU20931.1    AE014134    BT044571    AAF53307.1    ACI16533.1    GAMC01001550    JAC05006.1    CH954177    EDV58675.1    AY071533    AAL49155.1    DS235306    EEB14525.1    CH940654    EDW57502.1    KRF77798.1    UFQS01001230    UFQT01001230    SSX09858.1    SSX29581.1    UFQS01000039    UFQT01000039    SSW98095.1    SSX18481.1    JXJN01004961    GECZ01002874    JAS66895.1    GL448535    EFN84362.1    GEDC01029808    GEDC01024959    GEDC01003099    JAS07490.1    JAS12339.1    JAS34199.1    CH379058    EAL34391.1    CH479184    EDW37099.1    OUUW01000010    SPP85801.1    JTDY01001226    KOB74480.1    GEDC01006116    JAS31182.1    CH963857    EDW76094.1    GEBQ01028423    JAT11554.1    KK855543    PTY23050.1    CH902624    EDV33179.1    CH933807    EDW13455.1    KQ971361    EFA05143.1    KQ434850    KZC08553.1    CP012523    ALC39513.1    CH916368    EDW03178.1    GBHO01010160    GDHC01019476    JAG33444.1    JAP99152.1    GBRD01002659    GBRD01002658    GBRD01002657    JAG63162.1    AAZX01004497    AAZX01005555    AAZX01012331    AAZX01012371    AAZX01014385    GDRN01074797    JAI63173.1    GDRN01074798    JAI63172.1    QCYY01000760    ROT82901.1    GDIP01183397    JAJ40005.1    GDIQ01165083    GDIP01096558    JAK86642.1    JAM07157.1    GL732705    EFX66371.1    GDIQ01163690    JAK88035.1    GDIP01115802    JAL87912.1    GDIP01212950    GDIQ01251702    JAJ10452.1    JAK00023.1    GDIQ01086558    JAN08179.1    SPP85802.1    GDIP01049111    JAM54604.1    GDIP01054920    JAM48795.1    GDIP01047470    JAM56245.1    GDIP01028211    JAM75504.1    GDIQ01007792    JAN86945.1    GDIP01074215    JAM29500.1    GECU01029543    JAS78163.1    GBYB01008197    JAG77964.1    GDIQ01149742    JAL01984.1    GDIP01222745    JAJ00657.1    GGLE01002397    MBY06523.1    LJIJ01000043    ODN04442.1    GDIQ01102407    JAL49319.1    MNPL01002680    OQR78059.1    GDIP01056509    JAM47206.1    GFDF01002609    JAV11475.1    GBBL01001291    JAC26029.1    JO842486    AEO34103.1   

Proteomes

UP000005204    UP000053268    UP000218220    UP000007151    UP000283053    UP000053105    + More
UP000027135    UP000242457    UP000053825    UP000005203    UP000183832    UP000015103    UP000000311    UP000007755    UP000005205    UP000075809    UP000078492    UP000078541    UP000078540    UP000235965    UP000078542    UP000037069    UP000002282    UP000001292    UP000000304    UP000053097    UP000279307    UP000000803    UP000092445    UP000008711    UP000091820    UP000009046    UP000008792    UP000092443    UP000078200    UP000092460    UP000008237    UP000095301    UP000001819    UP000008744    UP000268350    UP000095300    UP000037510    UP000192221    UP000007798    UP000007801    UP000009192    UP000007266    UP000076502    UP000092553    UP000001070    UP000002358    UP000192223    UP000283509    UP000000305    UP000094527    UP000192247   

PRIDE

H9IVW5    Q9VJX8    Q8SYI0   

Pfam

PF04387   PTPLA        + More
PF04969   CS

Interpro

IPR007052   CS_dom        + More
IPR007482   Tyr_Pase-like_PTPLA       
IPR008978   HSP20-like_chaperone       

SUPFAM

SSF49764   SSF49764       

Gene 3D

2.60.40.790    

ProteinModelPortal

H9IVW5   A0A1E1WHM1   A0A194QDQ7   A0A2A4JQM1   A0A212F7A3   A0A3S2P7D4   + More
A0A0N0U7H8   A0A067QZZ2   A0A2A3EBK3   U5ENU0   A0A0L7R4H6   A0A088AFL9   A0A1J1IPI1   R4G8F4   A0A069DYT1   E2AFV5   F4WRF0   A0A158NUK9   A0A023F8E3   A0A151X6F6   A0A0V0G852   A0A224XSD5   A0A151JQ82   A0A195FJN5   A0A195BF81   A0A2J7R263   A0A151IFW3   A0A0L0BKW8   B4P373   B4IEM1   B4Q4U1   A0A026W5T6   Q9VJX8   A0A1A9Z9E1   W8BUI6   B3NAW1   A0A1A9VZR8   Q8SYI0   E0VMB9   B4M8H6   A0A1A9Y4Z3   A0A336MJD7   A0A1A9UYA3   A0A336K3R8   A0A1B0AWX9   A0A1B6GWX8   E2BIT0   A0A1I8MJA2   A0A1B6C2M9   Q29PA2   B4GKD3   A0A3B0KKX3   A0A1I8PN46   A0A0L7LGE2   A0A1W4UVF4   A0A1B6DZR9   B4MWF3   A0A1B6KK69   A0A2R7WV92   B3MVB4   B4KIF8   D2A528   A0A154P9N8   A0A0M3QTU3   B4JCT9   A0A0A9YMM9   A0A0K8TC72   K7J872   A0A0P4WFV7   A0A1W4WT73   A0A0P4WP27   A0A3R7PUF3   A0A0P5BWV1   A0A0N8BLU7   E9HPF0   A0A0P5M4B4   A0A0P5UDY0   A0A0P4ZSN7   A0A0P6DH87   A0A3B0JZE8   A0A0N8DBU2   A0A0P5YTF5   A0A0P5ZCI3   A0A0P6AKU7   A0A0P6JUY3   A0A0N8D2V4   A0A1B6HU10   A0A0C9R5Y1   A0A0P5MZ34   A0A0N7ZQE9   A0A2R5LAJ8   A0A1D2NGU6   A0A0P5SLZ6   A0A1V9XX68   A0A0P5YMP8   A0A1L8DYX2   A0A023FX35   G3MKT5  

PDB

2KMW     E-value=3.82818e-07,     Score=129

KEGG

101746115    K10703 very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase [EC:4.2.1.134] | (RefSeq) very-long-chain

PATHWAY

00062    Fatty acid elongation - Bombyx mori (domestic silkworm)
01040    Biosynthesis of unsaturated fatty acids - Bombyx mori (domestic silkworm)
01100    Metabolic pathways - Bombyx mori (domestic silkworm)
01212    Fatty acid metabolism - Bombyx mori (domestic silkworm)

GO

GO:0102343   GO:0102158   GO:0102345   GO:0005789   GO:0016021   GO:0102344   GO:0006633   GO:0005783   GO:0030497   GO:0019899   GO:0018812   GO:0042761   GO:0030148  

PANTHER

PTHR11035  

Subcellular location

Endoplasmic reticulum membrane  

Length:

372

Number of predicted TMHs:

4

Exp number of AAs in TMHs:

100.08474

Exp number, first 60 AAs:

0.00053

Total prob of N-in:

0.92022

inside

1  -  154

TMhelix

155  -  177

outside

178  -  219

TMhelix

220  -  237

inside

238  -  249

TMhelix

250  -  269

outside

270  -  327

TMhelix

328  -  350

inside

351  -  372

 

 

Pi

26.794426

Theta

23.117622

Tajima's D

0.490976

CLR

0.991607

CSRT

0.521173941302935

Interpretation

Uncertain