Gene

KWMTBOMO12604

Pre Gene Modal

BGIBMGA001548

Annotation

PREDICTED:_tyrosine-protein_kinase_CSK_isoform_X1_[Bombyx_mori]

Location

Bomo_Chr21(+):9158050-9197714      View in EpiBrowser

Full name

Tyrosine-protein kinase       + More
Tyrosine-protein kinase CSK      

Alternative Name

C-Src kinase
Protein-tyrosine kinase MPK-2
p50CSK

Location in the cell

Nuclear   Reliability : 2.402

CDS

ATGAATTCTGACGTGCATCGTCATCAGGCGCATCCGCCATTGGCACAGCACGGAGTGCACGGTGCGGCGCAGGGCGTGGGCCCTTGTGGCTGGTATTCGGCTTCTGCCGCTCCTGCACCCACGGCCCCTGCTCGTCTTAAGCGCGTACAAGCACCTCATCAGCTACCGTCTGGAGGGAACTTGAGCGGCAGTAATGGCCCGCACGCGCCTCTCTCCCCGTCCGCATTGCAGAATACTATACCGCACAATCTTATAATGTCATCGTCAGTCCGGACACCCGCTCCGGCCGCCACTGTACCGTCCGCCATGCACACCTCGCAAACAGCACCAGCAGTCACGAAAGGACAGAGCGATGTCTTGGTAAACTCGTCCGTACAGAACCCACCGCCGGCCGGCAACAGGCTGGTCAATATGACACAGTGGCCATGGCATCACGGCGCGATATCGAGGGAGCGAGCGGAGGCGTTGCTCTCCGGCTCTCCGGACGGGGTTTTCTTGGTGCGAGAGAGCACTAACTTCCCAGGAGACCACACGCTGTGCGTCCGATTCCGAGGAAGGGTGGAACACTATAGAGTAAAATGGGCGACAGCGCCCGACAACCAAAACCAACGTCTGACCATAGACGATGAAGAATTCTTCGATAACATGACGGACTTAATTCATCACTACCTGCAGGACGCGGACGGGCTGTGCACGAAGCTGGTGCGGTGCCTCCCCAAGGCGACGGCGAACGGCACCAACAACAACGGCGCCCCGCAGCAGCAGTACCCGCCCCACCCGCAGCAGCCGCCGCCGTATCCGCCCACGCCGAGCGTGCCGAGCGCGCTGGCGGCGGGACACTTCCCGCACGCGTACGTCGCGCCGGCCGCCTCCACCGACCAGACGGACGGCACCTTGCAGTCGCAAAGCTTCGTTGACGCGCGTTGGAAAATAGACGAGCGCGACCTTGAAATCCGAGAAAATATCGGCAAAGGAGAATTCGGCGACGTGATGCTAGGGATCCTGAACGGCACGCAGAAAGTGGCCGTCAAAATTCTCAAAGATCGGGAAGCCGCTAGTAAATTCCGCGCTGAAGCTAGTGTGATGGCATCGTTGAAGCACGAGAACCTGGTCCGTCTGCTGGGGCTGGTGTTCACGAGCAGCGGCGGCACGTGCCTGGTGACGGAGCACTGCGCGCAGGGCTCGCTGCTCGACTACCTGCGCAGCCGCGGCCGCCACTACGTCACGCAGCTCAACCAGATCAACTTCGCGTTTGACGCGTGCTGTGGGATGGAATATTTAGAAAGGCAACGTGTCGTACACCGCGACCTGGCGGCGAGAAATGTGCTCATCTCGGCGGAAGGCACCGCCAAGGTGGCGGACTTCGGTCTGGCGCGAGCTGGTGCAGCGCTGGAGGATCCGGCGGAGGCGGCTCGGCTACAGGCCAAGTTGCCCATTAAATGGACAGCACCGGAGGCACTTAAATATAACAAATTCTCAAACAAGTCGGATATGTGGAGTTTTGGAATTTTATTATGGGAAATTTACTCGTTTGGAAGAGTACCTTATCCTAGAATTCCGCTGGCGGAGGTGGTGCGGCACGTGGAGCGCGGCTACCGCATGGAGGCGCCGGAGGGGTGTCCGCCCGGCCCCTACGAGCTCATGCGCGCCGCCTGGCACGCCGACCCCGCCTCGCGACCCACCTTCCTGCACATGCGCCGCAACCTCGCCGACATACGCGACCACACGCCCGCGCAGGCTGATGATGATGATGATTCGCTGGTAGCCTAA

Protein

MNSDVHRHQAHPPLAQHGVHGAAQGVGPCGWYSASAAPAPTAPARLKRVQAPHQLPSGGNLSGSNGPHAPLSPSALQNTIPHNLIMSSSVRTPAPAATVPSAMHTSQTAPAVTKGQSDVLVNSSVQNPPPAGNRLVNMTQWPWHHGAISRERAEALLSGSPDGVFLVRESTNFPGDHTLCVRFRGRVEHYRVKWATAPDNQNQRLTIDDEEFFDNMTDLIHHYLQDADGLCTKLVRCLPKATANGTNNNGAPQQQYPPHPQQPPPYPPTPSVPSALAAGHFPHAYVAPAASTDQTDGTLQSQSFVDARWKIDERDLEIRENIGKGEFGDVMLGILNGTQKVAVKILKDREAASKFRAEASVMASLKHENLVRLLGLVFTSSGGTCLVTEHCAQGSLLDYLRSRGRHYVTQLNQINFAFDACCGMEYLERQRVVHRDLAARNVLISAEGTAKVADFGLARAGAALEDPAEAARLQAKLPIKWTAPEALKYNKFSNKSDMWSFGILLWEIYSFGRVPYPRIPLAEVVRHVERGYRMEAPEGCPPGPYELMRAAWHADPASRPTFLHMRRNLADIRDHTPAQADDDDDSLVA

Description

Non-receptor tyrosine-protein kinase that plays an important role in the regulation of cell growth, differentiation, migration and immune response. Phosphorylates tyrosine residues located in the C-terminal tails of Src-family kinases (SFKs) including LCK, SRC, HCK, FYN, LYN, CSK or YES1. Upon tail phosphorylation, Src-family members engage in intramolecular interactions between the phosphotyrosine tail and the SH2 domain that result in an inactive conformation. To inhibit SFKs, CSK is recruited to the plasma membrane via binding to transmembrane proteins or adapter proteins located near the plasma membrane. Suppresses signaling by various surface receptors, including T-cell receptor (TCR) and B-cell receptor (BCR) by phosphorylating and maintaining inactive several positive effectors such as FYN or LCK (By similarity).

Catalytic Activity

ATP + L-tyrosyl-[protein] = ADP + H(+) + O-phospho-L-tyrosyl-[protein]

Subunit

Homodimer (via SH3-domain) (By similarity). Interacts with PTPN22 (PubMed:8890164). Interacts with phosphorylated SIT1, PAG1, LIME1 and TGFB1I1; these interactions serve to recruit CSK to the membrane where it can phosphorylate and inhibit Src-family kinases (PubMed:9858471, PubMed:12218089, PubMed:12612075, PubMed:16166631). Interacts with SRCIN1 (By similarity). Interacts with RHOH (By similarity). Interacts (via SH2 domain) with SCIMP (By similarity). Interacts (via SH2 domain) with PRAG1 (when phosphorylated at 'Tyr-391'); this interaction prevents translocation of CSK from the cytoplasm to the membrane leading to increased activity of CSK (By similarity).
Homodimer (via SH3-domain). Interacts with PTPN22. Interacts with phosphorylated SIT1, PAG1, LIME1 and TGFB1I1; these interactions serve to recruit CSK to the membrane where it can phosphorylate and inhibit Src-family kinases. Interacts with SRCIN1. Interacts with RHOH. Interacts (via SH2 domain) with SCIMP (By similarity). Interacts (via SH2 domain) with PRAG1 (when phosphorylated at 'Tyr-391'); this interaction prevents translocation of CSK from the cytoplasm to the membrane leading to increased activity of CSK (PubMed:21873224).

Similarity

Belongs to the protein kinase superfamily. Tyr protein kinase family.
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily.

Keywords

3D-structure   Acetylation   Adaptive immunity   ATP-binding   Cell membrane   Complete proteome   Cytoplasm   Direct protein sequencing   Immunity   Kinase   Magnesium   Manganese   Membrane   Metal-binding   Nucleotide-binding   Phosphoprotein   Reference proteome   SH2 domain   SH3 domain   Transferase   Tyrosine-protein kinase  

Feature

chain  Tyrosine-protein kinase CSK

Uniprot

H9IWB7   A0A2A4JS35   A0A194Q2D8   A0A212ERP4   A0A2H1VDH0   A0A2A4JQK7   + More
A0A3S2M928   A0A2J7RIA3   A0A067QVQ7   A0A1B6JEY6   A0A1B6DC98   A0A1B6GL01   A0A023F4U1   A0A0V0GBU4   A0A224XRI6   A0A069DU21   A0A2R7WMY9   U5EGS0   B4JFH9   A0A088AI81   A0A2A3EBE0   A0A0A9YK91   A0A0L7QKJ6   A0A158N9Q5   A0A195F8C8   E0VB13   A0A195BNP3   E2BQA4   A0A151ISY3   F4WRI0   A0A151IC38   A0A151XGX3   A0A154PQC7   A0A1D2NB97   B4M474   A0A026WH98   E2AGI3   A0A0J7LBA3   E9J0V9   A0A0P6HX98   B3M100   A0A0P8Y024   A0A310S809   A0A2H8TCW2   J9KAY7   A0A1Y1LC76   A0A0N8DPS2   A0A076FKY1   L7M1U7   A0A224YUT6   A0A131YWK3   A0A131XCJ3   T1JDP9   A0A131XXI8   A0A087UEP1   A0A226F1H3   V5H7J5   A0A1E1XQP7   L7MKD7   A0A1E1X8H3   A0A2L2YK04   A0A2R5L9A0   A0A1Z5KWJ4   A0A443RF52   A0A443RF28   A0A232EXM9   A0A0P4VYG5   A0A1S3ICC4   A0A1V9X5D6   A0A443SQH9   Q3UVH2   A0A1D1V0P3   A0A1U7QKS8   M3VUK8   G3IIC0   A0A2U3Y9U0   A0A2Y9KEJ2   A0A3P4KYT3   M3Z0S9   G3WSI5   E2RFF7   A0A3Q7RDQ4   A0A3Q7RFS7   A0A340WB71   P41241   A0A2U3Y9U7   D2HKY4   A0A452FQZ5   A0A2Y9HIQ2   A0A340WGT5   A0A452SQW6   A0A3Q7TEX9   P32577   A0A2K6GVY3   A0A2Y9DHG3   F6QS08   A0A2U3WGL3  

EC Number

2.7.10.2

Pubmed

19121390    26354079    22118469    24845553    25474469    26334808    + More
17994087    25401762    26823975    21347285    20566863    20798317    21719571    27289101    24508170    30249741    21282665    28004739    25576852    28797301    26830274    28049606    25765539    29209593    28503490    26561354    28528879    28648823    28327890    10349636    11042159    11076861    11217851    12466851    16141073    27649274    17975172    21804562    23236062    21709235    16341006    7511815    16141072    15489334    1281307    7685657    8890164    9858471    12218089    12612075    16166631    21183079    11685249    20010809    1709258    1722201    7515063    10801129    14613929    21873224    25243066   

EMBL

BABH01006344    BABH01006345    NWSH01000780    PCG74283.1    KQ459564    KPI99706.1    + More
AGBW02012992    OWR44121.1    ODYU01001967    SOQ38889.1    PCG74281.1    RSAL01000006    RVE54310.1    NEVH01003502    PNF40560.1    KK852898    KDR14137.1    GECU01031354    GECU01016690    GECU01010018    JAS76352.1    JAS91016.1    JAS97688.1    GEDC01026840    GEDC01013981    JAS10458.1    JAS23317.1    GECZ01029194    GECZ01016012    GECZ01006698    JAS40575.1    JAS53757.1    JAS63071.1    GBBI01002231    JAC16481.1    GECL01000561    JAP05563.1    GFTR01005805    JAW10621.1    GBGD01001697    JAC87192.1    KK855120    PTY20988.1    GANO01003329    JAB56542.1    CH916369    EDV93460.1    KZ288293    PBC29045.1    GBHO01042297    GBHO01042296    GBHO01042295    GBHO01037590    GBHO01037573    GBHO01027716    GBHO01027715    GBHO01027712    GBHO01027711    GBHO01025319    GBHO01025318    GBHO01025316    GBHO01025315    GBHO01013653    GBHO01013651    GBHO01013650    GBHO01013649    GBHO01013648    GBRD01008929    GBRD01008928    GDHC01013034    GDHC01007928    JAG01307.1    JAG01308.1    JAG01309.1    JAG06014.1    JAG06031.1    JAG15888.1    JAG15889.1    JAG15892.1    JAG15893.1    JAG18285.1    JAG18286.1    JAG18288.1    JAG18289.1    JAG29951.1    JAG29953.1    JAG29954.1    JAG29955.1    JAG29956.1    JAG56892.1    JAQ05595.1    JAQ10701.1    KQ414982    KOC59026.1    ADTU01009736    ADTU01009737    KQ981727    KYN36636.1    AAZO01000572    DS235020    EEB10569.1    KQ976428    KYM87842.1    GL449698    EFN82158.1    KQ981064    KYN09871.1    GL888287    EGI63197.1    KQ978067    KYM97655.1    KQ982169    KYQ59548.1    KQ435026    KZC13937.1    LJIJ01000108    ODN02521.1    CH940652    EDW59435.2    KK107211    QOIP01000005    EZA55338.1    RLU22694.1    GL439316    EFN67440.1    LBMM01000061    KMR05118.1    GL767538    EFZ13551.1    GDIP01187589    GDIQ01028461    LRGB01000642    JAJ35813.1    JAN66276.1    KZS17359.1    CH902617    EDV43229.2    KPU80147.1    KQ770716    OAD52628.1    GFXV01000151    MBW11956.1    ABLF02030156    GEZM01060095    GEZM01060094    JAV71269.1    GDIP01012871    JAM90844.1    KF516677    AII16581.1    GACK01006733    JAA58301.1    GFPF01006434    MAA17580.1    GEDV01005529    JAP83028.1    GEFH01004736    JAP63845.1    JH432107    GEFM01004861    JAP70935.1    KK119489    KFM75830.1    LNIX01000001    OXA62786.1    GANP01005373    JAB79095.1    GFAA01002163    JAU01272.1    GACK01000747    JAA64287.1    GFAC01003680    JAT95508.1    IAAA01036060    IAAA01036061    LAA08458.1    GGLE01001955    MBY06081.1    GFJQ02007511    JAV99458.1    NCKU01000860    RWS13873.1    NCKU01000861    RWS13870.1    NNAY01001730    OXU23103.1    GDRN01107837    JAI57365.1    MNPL01023305    OQR68809.1    NCKV01000784    RWS29753.1    AK137297    BAE23297.1    BDGG01000002    GAU93147.1    AANG04001761    JH002978    EGW15026.1    CYRY02001003    VCW55680.1    AEYP01046596    JP008159    AER96756.1    AEFK01001178    AEFK01001179    AEFK01001180    AAEX03016316    AAEX03016317    AAEX03016318    U05247    AY902339    AK156058    AK170815    BC018394    BC052006    X57242    ACTA01108031    ACTA01116031    GL192984    EFB20241.1    LWLT01000019    X58631    BC098863    GAMT01001202    GAMS01000600    GAMR01007519    GAMQ01006009    GAMP01004935    JAB10659.1    JAB22536.1    JAB26413.1    JAB35842.1    JAB47820.1   

Proteomes

UP000005204    UP000218220    UP000053268    UP000007151    UP000283053    UP000235965    + More
UP000027135    UP000001070    UP000005203    UP000242457    UP000053825    UP000005205    UP000078541    UP000009046    UP000078540    UP000008237    UP000078492    UP000007755    UP000078542    UP000075809    UP000076502    UP000094527    UP000008792    UP000053097    UP000279307    UP000000311    UP000036403    UP000076858    UP000007801    UP000007819    UP000054359    UP000198287    UP000285301    UP000215335    UP000085678    UP000192247    UP000288716    UP000186922    UP000189706    UP000011712    UP000001075    UP000245341    UP000248482    UP000000715    UP000007648    UP000002254    UP000286640    UP000286641    UP000265300    UP000000589    UP000008912    UP000291000    UP000248481    UP000291022    UP000286642    UP000002494    UP000233160    UP000248480    UP000008225    UP000245340   

PRIDE

H9IWB7    Q3UVH2    G3IIC0    E2RFF7    P41241    P32577    + More

Pfam

PF00017   SH2        + More
PF07714   Pkinase_Tyr
PF00018   SH3_1
PF14796   AP3B1_C
PF01602   Adaptin_N

Interpro

IPR036860   SH2_dom_sf        + More
IPR020635   Tyr_kinase_cat_dom       
IPR008266   Tyr_kinase_AS       
IPR000719   Prot_kinase_dom       
IPR000980   SH2       
IPR011009   Kinase-like_dom_sf       
IPR001245   Ser-Thr/Tyr_kinase_cat_dom       
IPR017441   Protein_kinase_ATP_BS       
IPR035027   Csk-like_SH2       
IPR001452   SH3_domain       
IPR036028   SH3-like_dom_sf       
IPR026739   AP_beta       
IPR016024   ARM-type_fold       
IPR002553   Clathrin/coatomer_adapt-like_N       
IPR029390   AP3B_C       
IPR011989   ARM-like       

SUPFAM

SSF55550   SSF55550        + More
SSF56112   SSF56112       
SSF50044   SSF50044       
SSF48371   SSF48371       

Gene 3D

3.30.505.10     1.25.10.10    

CDD

cd09937  SH2_csk_like

ProteinModelPortal

H9IWB7   A0A2A4JS35   A0A194Q2D8   A0A212ERP4   A0A2H1VDH0   A0A2A4JQK7   + More
A0A3S2M928   A0A2J7RIA3   A0A067QVQ7   A0A1B6JEY6   A0A1B6DC98   A0A1B6GL01   A0A023F4U1   A0A0V0GBU4   A0A224XRI6   A0A069DU21   A0A2R7WMY9   U5EGS0   B4JFH9   A0A088AI81   A0A2A3EBE0   A0A0A9YK91   A0A0L7QKJ6   A0A158N9Q5   A0A195F8C8   E0VB13   A0A195BNP3   E2BQA4   A0A151ISY3   F4WRI0   A0A151IC38   A0A151XGX3   A0A154PQC7   A0A1D2NB97   B4M474   A0A026WH98   E2AGI3   A0A0J7LBA3   E9J0V9   A0A0P6HX98   B3M100   A0A0P8Y024   A0A310S809   A0A2H8TCW2   J9KAY7   A0A1Y1LC76   A0A0N8DPS2   A0A076FKY1   L7M1U7   A0A224YUT6   A0A131YWK3   A0A131XCJ3   T1JDP9   A0A131XXI8   A0A087UEP1   A0A226F1H3   V5H7J5   A0A1E1XQP7   L7MKD7   A0A1E1X8H3   A0A2L2YK04   A0A2R5L9A0   A0A1Z5KWJ4   A0A443RF52   A0A443RF28   A0A232EXM9   A0A0P4VYG5   A0A1S3ICC4   A0A1V9X5D6   A0A443SQH9   Q3UVH2   A0A1D1V0P3   A0A1U7QKS8   M3VUK8   G3IIC0   A0A2U3Y9U0   A0A2Y9KEJ2   A0A3P4KYT3   M3Z0S9   G3WSI5   E2RFF7   A0A3Q7RDQ4   A0A3Q7RFS7   A0A340WB71   P41241   A0A2U3Y9U7   D2HKY4   A0A452FQZ5   A0A2Y9HIQ2   A0A340WGT5   A0A452SQW6   A0A3Q7TEX9   P32577   A0A2K6GVY3   A0A2Y9DHG3   F6QS08   A0A2U3WGL3  

PDB

1K9A     E-value=5.91835e-112,     Score=1035

KEGG

101739746    K05728 c-src tyrosine kinase [EC:2.7.10.2] | (RefSeq) tyrosine-protein kinase CSK

GO

GO:0005524   GO:0004715   GO:0045197   GO:0045571   GO:0048167   GO:0007293   GO:0008285   GO:0034334   GO:0035332   GO:0016477   GO:0043068   GO:0010669   GO:0016192   GO:0006886   GO:0030117   GO:0005886   GO:0042802   GO:0042997   GO:0034236   GO:0005911   GO:0034332   GO:0060368   GO:0010989   GO:0033673   GO:0045779   GO:0007420   GO:0046872   GO:0006468   GO:0070373   GO:0005737   GO:0050765   GO:0070064   GO:0046777   GO:0035556   GO:0004713   GO:0045121   GO:0016740   GO:0048709   GO:0050863   GO:0019903   GO:0071375   GO:0043406   GO:0032715   GO:0002250   GO:0004672   GO:0030286   GO:0008270   GO:0016787   GO:0004190   GO:0006508   GO:0016286  

PANTHER

PTHR11134  

Subcellular location

Cytoplasm  
Cell membrane  

Length:

589

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.03775

Exp number, first 60 AAs:

0.00796

Total prob of N-in:

0.00358

outside

1  -  589

 

 

Pi

259.21564

Theta

173.741035

Tajima's D

1.254249

CLR

0.312771

CSRT

0.727963601819909

Interpretation

Uncertain