Gene

KWMTBOMO12496

Pre Gene Modal

BGIBMGA001467

Annotation

PREDICTED:_ubiquitin-like_modifier-activating_enzyme_ATG7_[Amyelois_transitella]

Location

Bomo_Chr21(-):5016797-5026524      View in EpiBrowser

Full name

Ubiquitin-like modifier-activating enzyme ATG7      

Alternative Name

ATG12-activating enzyme E1 ATG7
Autophagy-related protein 7
Ubiquitin-activating enzyme E1-like protein

Location in the cell

PlasmaMembrane   Reliability : 2.325

CDS

ATGTTGGAAGCACAAAACCAAACAGAAATAATACAGTATGTGCCGTTTTCGTCTTTCGTTCATCCATCATTCTGGCATACCCTCACTGAAATGAAATTAGAAGTAGACAAATTAAAGGAAACTACCAAACAAATATTCGGCAGATTTACATACCGCTGTGATATTGGCTCTGTATTTGAAGTGGATGGTACCTCATTCAATAAGACACCTCATTTAGAGCAACAATATCATCATGTGATGGGTACAATAATGAACAAAAACACTATTGAAGACTTCAAATCTATAGATAAGGCTTCATTACTAAATTCAATAGGCGAAATGATATGGTCCAACCTCAGGGAGAGAACTTGGATCACAAATCCTAGTGCCTTGTTGAATTTCTTCATACTCTCATTTGCTGATCTCAAAAAATTTCATTATTACTACTGGTTTGCGTTTCCTGCACCCAGCCAACCCACCGTTCACATGAAAGGAAGAAGCACGAAAATCTCAGATTACTTCAACAACAAACAATTAGAAACTCTATCTCAATGTTACAAATCACTTGAAGAAAACCAAAAGAACTTTTTTGTTGTAATCAAGAAAAATGATGATCTCTCAGTGAAGAAATTATCAGAAGTTTTCGATGTTAATAGTGCTAACTGCATTGACCTAGATCTCGTCAGTACATACTTTGTTTTTGCTGATCCAAGTAATGGATGCAACCCTGGTTGGCCACTAAGAACATTCTTAGCCGCACTGTTGGAATATTGTCCTGAATTGGCAAAGTCAACTTTACAAGTTATAGGGTTGAGGAGTTCAATGAATGGTGATTTCATAAAAAGCCTAGTTTTCTCCATTGAAATTCCACAGGACATCAAGCCAGTTGAGAGTGCTGGTTGGGTAGGATGGGAAAGAAACGATAAAGGCAATTTTGGACCCAGGCTCGCTAATATGTCTACCTCGATGGATCCTGTCATATTGGCAGATACGTCATCAGATCTGAATATAAAGTTGATGAAATGGCGTCTAGTACCTGATCTGAATGTAGGTGTGATGAAAGATACGAAATGTTTGTTATTGGGAGCCGGTACACTTGGTTGTCACGTGGCGAGAAATTTATTAGCCTGGGGTTTCCGTCATATAACATTCGTTGACAACGGTAAAGTGTCATACTCGAATCCGACCAGACAAGTTCTGTTCAACTATCAAGATTGTCTCGGGGGAGGCCGAAGGAAAGCCGAAGCGGCCGCTGATAACTTGAAGTCAATCCTCCCGACTACTAATAGCAAAGGTATAGTAGCGCACATACCGATGCCAGGACATCCTATTGGGGATTCATTGAAAGAGGAGACCATTGGCGATATCAAAAGGATAACCGAGGCTATCTCTGAACACGACGTCGTGTTCCTTCTTCTGGACACGAGGGAGGCGAGATGGCTGCCAACACTCATTGCAGCACAACACCGGAAGATCGTTATAAATGCGGCGTTAGGTTTCGACAGTTATCTAGTGATGCGACACGGAATTAGCACCTCGTCAGAAGAGGTCGGTACTCTCGATAAACAATACATCGAAGGCCGGTACCTCGGTTGCTATTTCTGTAATGACGTCACCGCACCTGGCAATTCACTGAGAGATCGCACGTTAGATCAACAGTGCACCGTGACCCGCCCCGGGGTCGCGGCGGTGGCCGGCGCTCTGTCCGTCGAGATACTGGTGGCGTTGTTGCAGCATCCGAAAAGAGTCGATGCCCCGGCCCTGTACAATTTTAATAAGACCGAACAAGAGATTCCGTCGCAAATCGAAGGAGTCTTGGGCGCCGTACCTCATTCGATAAGAGGATTCCTGCATTCGTACCAAACGATCGCACCGACATGCACGAAATTCAAACAGTGCATCGCGTGCTCCGACACTGTGATAAACAAGTACAGAGAGGAAGGATTGGATTTCCTTCTGAACGTATTCAACAGCGGTAGTTATTTGGAAGAAGTAACTGGCTTATCGGCTTTGCATTTAAGCGCTGAAATGAGCGAGATATTGACATTAACCGATGAAGAAGATTAG

Protein

MLEAQNQTEIIQYVPFSSFVHPSFWHTLTEMKLEVDKLKETTKQIFGRFTYRCDIGSVFEVDGTSFNKTPHLEQQYHHVMGTIMNKNTIEDFKSIDKASLLNSIGEMIWSNLRERTWITNPSALLNFFILSFADLKKFHYYYWFAFPAPSQPTVHMKGRSTKISDYFNNKQLETLSQCYKSLEENQKNFFVVIKKNDDLSVKKLSEVFDVNSANCIDLDLVSTYFVFADPSNGCNPGWPLRTFLAALLEYCPELAKSTLQVIGLRSSMNGDFIKSLVFSIEIPQDIKPVESAGWVGWERNDKGNFGPRLANMSTSMDPVILADTSSDLNIKLMKWRLVPDLNVGVMKDTKCLLLGAGTLGCHVARNLLAWGFRHITFVDNGKVSYSNPTRQVLFNYQDCLGGGRRKAEAAADNLKSILPTTNSKGIVAHIPMPGHPIGDSLKEETIGDIKRITEAISEHDVVFLLLDTREARWLPTLIAAQHRKIVINAALGFDSYLVMRHGISTSSEEVGTLDKQYIEGRYLGCYFCNDVTAPGNSLRDRTLDQQCTVTRPGVAAVAGALSVEILVALLQHPKRVDAPALYNFNKTEQEIPSQIEGVLGAVPHSIRGFLHSYQTIAPTCTKFKQCIACSDTVINKYREEGLDFLLNVFNSGSYLEEVTGLSALHLSAEMSEILTLTDEED

Description

E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Plays also a key role in the maintenance of axonal homeostasis, the prevention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation. Plays a role in regulating the liver clock and glucose metabolism by mediating the autophagic degradation of CRY1 (clock repressor) in a time-dependent manner (By similarity).

Subunit

Homodimer. Interacts with ATG3 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5. Forms intermediate conjugates with ATG8-like proteins such as GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A. Interacts with EP300 acetyltransferase. Interacts with FOXO1.

Similarity

Belongs to the ATG7 family.

Keywords

Acetylation   Alternative splicing   Autophagy   Biological rhythms   Complete proteome   Cytoplasm   Phosphoprotein   Polymorphism   Protein transport   Reference proteome   Transport   Ubl conjugation pathway  

Feature

chain  Ubiquitin-like modifier-activating enzyme ATG7
splice variant  In isoform 3.
sequence variant  In dbSNP:rs36117895.

Uniprot

H9IW36   A0A2H1WH22   A0A437BQE0   A0A2A4K5C7   A0A212F8A5   A0A2H1WUX5   + More
A0A194Q4R6   A0A1Y1NIW5   A0A1B0CFH4   B3NMV3   Q7JY94   B4J519   A0A3Q8CR88   B4LM30   A0A0M3QV36   B4QD00   A0A0J9RG05   A0A1W4VR37   B4KSU5   B3MDE8   B4HNY7   B4P4B9   A0A0A1WUB6   E0VRG5   B4GH12   A0A336KNP1   Q28ZN4   A0A3B0J2W1   A0A034VL57   A0A3B0J2E3   A0A336LU38   A0A0K8UC96   A0A139WBZ1   B4MQY6   W8BMG7   A0A1I8MQR0   T1PH22   A0A1J1HY84   A0A1I8NNH4   A0A1B6EJ80   A0A1W4XI33   A0A0N7ZA03   R7TQM1   A0A1B6E4U1   A0A0L8FZ62   D3TP22   A0A210PF26   A0A1B0C3S0   A0A1Z5LG49   A0A3S1BLC1   A0A2R5LNM6   A0A1B0A4Q3   A0A3M6TT48   A0A197JNY9   A0A1S3IB82   A0A397SG99   A0A2Z4EUI1   A0A293N471   A0A286XDX7   A0A1S4FNL7   A0A087TQT1   A0A0N8ETI2   Q16UR2   A0A2Z4EUN1   A0A0P5PKS8   A0A2Z4EUL1   T2MHR4   A0A0P5N015   A0A0P6DH27   E9GCT9   I3MPI3   A0A3M7RK75   A0A0P5SQA0   J9K7P8   B3KQM6   O95352   G7NYH8   F7ESQ7   A0A0T6B3D2   A0A0C9V069   A0A2R9C9V9   H2QM24   A0A0C9VA15   A0A023EVS1   A0A1D2N3H5   A0A2K6B8E2   A0A2I2ZVZ4   A0A0D9RBZ0   A0A2J8WDD9   B2RB57   F6TZ29   A0A1W2W0C6   A0A0P5J8H3   A0A287CXQ2   A0A250YKQ9   K7DKQ6   A0A2K5XUN0   A0A2K5MAK2   A0A2K6T6D4   A0A2K6FZ84  

Pubmed

19121390    22118469    26354079    28004739    17994087    10731132    + More
12537568    12537572    12537573    12537574    16110336    17569856    17569867    26109357    26109356    22936249    17550304    25830018    20566863    15632085    25348373    18362917    19820115    24495485    25315136    23254933    20353571    28812685    28528879    30382153    29885584    21993624    17510324    24065732    21292972    30375419    16303743    10233149    14702039    16641997    15489334    17974005    11096062    11890701    11825910    16303767    19413330    19124466    19690332    20543840    20068231    21269460    22170151    22223895    23386620    24275569    22002653    17431167    25319552    22722832    16136131    24945155    27289101    22398555    12481130    15114417    28087693   

EMBL

BABH01005936    ODYU01008628    SOQ52370.1    RSAL01000020    RVE52601.1    NWSH01000166    + More
PCG78882.1    AGBW02009774    OWR49953.1    ODYU01011243    SOQ56860.1    KQ459585    KPI98400.1    GEZM01006605    JAV95687.1    AJWK01010106    CH954179    EDV55107.1    AE013599    AY084144    AAF57665.1    AAL89882.1    CH916367    EDW01725.1    KY053223    ATX63058.1    CH940648    EDW59950.1    CP012524    ALC41729.1    CM000362    EDX07705.1    CM002911    KMY94892.1    CH933808    EDW08442.1    CH902619    EDV36396.1    CH480816    EDW48489.1    CM000158    EDW91605.1    GBXI01012076    JAD02216.1    DS235465    EEB15971.1    CH479183    EDW35782.1    UFQS01000732    UFQT01000732    SSX06456.1    SSX26805.1    CM000071    EAL25579.4    OUUW01000001    SPP75575.1    GAKP01016694    JAC42258.1    SPP75574.1    UFQT01000194    SSX21445.1    GDHF01028103    GDHF01025080    JAI24211.1    JAI27234.1    KQ971371    KYB25457.1    CH963849    EDW74525.1    GAMC01011989    JAB94566.1    KA648071    AFP62700.1    CVRI01000036    CRK92981.1    GECZ01031818    JAS37951.1    GDRN01107011    JAI57488.1    AMQN01012821    KB309782    ELT93310.1    GEDC01004342    JAS32956.1    KQ425160    KOF69874.1    EZ423174    ADD19450.1    NEDP02076741    OWF35095.1    JXJN01025096    GFJQ02000986    JAW05984.1    RQTK01001576    RUS69787.1    GGLE01006978    MBY11104.1    RCHS01002978    RMX44511.1    KV442062    OAQ26875.1    QKYT01000435    RIA85270.1    MH231833    AWV66640.1    GFWV01022271    MAA46998.1    AAKN02052979    KK116336    KFM67470.1    GEBF01004307    JAN99325.1    CH477615    EAT38276.1    MH231881    AWV66688.1    GDIQ01127519    JAL24207.1    MH231857    AWV66664.1    HAAD01005407    CDG71639.1    GDIQ01148966    JAL02760.1    GDIQ01086640    JAN08097.1    GL732539    EFX82614.1    AGTP01073366    AGTP01073367    AGTP01073368    AGTP01073369    REGN01003213    RNA23800.1    GDIP01140182    LRGB01000944    JAL63532.1    KZS14521.1    ABLF02035107    AK075221    BAG52088.1    AF094516    AK303694    AC020750    AC022001    AC026185    AC083855    BC000091    AL122075    AQIA01040408    AQIA01040409    AQIA01040410    AQIA01040411    AQIA01040412    CM001277    EHH51137.1    JSUE03023197    JSUE03023198    JSUE03023199    JSUE03023200    JU321460    CM001254    AFE65216.1    EHH16173.1    LJIG01015998    KRT81920.1    KN837144    KIJ40484.1    AJFE02092327    AJFE02092328    AJFE02092329    AJFE02092330    AJFE02092331    AJFE02092332    AJFE02092333    AJFE02092334    AJFE02092335    AACZ04000084    AACZ04000085    KN837120    KIJ43809.1    GAPW01000411    JAC13187.1    LJIJ01000253    ODM99826.1    CABD030019875    CABD030019876    CABD030019877    CABD030019878    CABD030019879    CABD030019880    CABD030019881    CABD030019882    CABD030019883    CABD030019884    AQIB01144071    AQIB01144072    AQIB01144073    AQIB01144074    AQIB01144075    AQIB01144076    NDHI03003394    PNJ67783.1    AK314504    BAG37104.1    EAAA01001413    GDIQ01203750    JAK47975.1    GFFW01000555    JAV44233.1    GABC01006814    GABF01002179    GABD01005843    GABE01004069    NBAG03000274    JAA04524.1    JAA19966.1    JAA27257.1    JAA40670.1    PNI53312.1   

Proteomes

UP000005204    UP000283053    UP000218220    UP000007151    UP000053268    UP000092461    + More
UP000008711    UP000000803    UP000001070    UP000008792    UP000092553    UP000000304    UP000192221    UP000009192    UP000007801    UP000001292    UP000002282    UP000009046    UP000008744    UP000001819    UP000268350    UP000007266    UP000007798    UP000095301    UP000183832    UP000095300    UP000192223    UP000014760    UP000053454    UP000242188    UP000092460    UP000271974    UP000092445    UP000275408    UP000078512    UP000085678    UP000265703    UP000005447    UP000054359    UP000008820    UP000000305    UP000005215    UP000276133    UP000076858    UP000007819    UP000005640    UP000009130    UP000233100    UP000006718    UP000240080    UP000002277    UP000094527    UP000233120    UP000001519    UP000029965    UP000008144    UP000233140    UP000233060    UP000233220    UP000233160   

PRIDE

H9IW36    Q7JY94    B3KQM6    O95352    B2RB57   

Pfam

PF00899   ThiF        + More
PF16420   ATG7_N

Interpro

IPR006285   Atg7        + More
IPR000594   ThiF_NAD_FAD-bd       
IPR035985   Ubiquitin-activating_enz       
IPR032197   Atg7_N       

SUPFAM

SSF69572   SSF69572       

ProteinModelPortal

H9IW36   A0A2H1WH22   A0A437BQE0   A0A2A4K5C7   A0A212F8A5   A0A2H1WUX5   + More
A0A194Q4R6   A0A1Y1NIW5   A0A1B0CFH4   B3NMV3   Q7JY94   B4J519   A0A3Q8CR88   B4LM30   A0A0M3QV36   B4QD00   A0A0J9RG05   A0A1W4VR37   B4KSU5   B3MDE8   B4HNY7   B4P4B9   A0A0A1WUB6   E0VRG5   B4GH12   A0A336KNP1   Q28ZN4   A0A3B0J2W1   A0A034VL57   A0A3B0J2E3   A0A336LU38   A0A0K8UC96   A0A139WBZ1   B4MQY6   W8BMG7   A0A1I8MQR0   T1PH22   A0A1J1HY84   A0A1I8NNH4   A0A1B6EJ80   A0A1W4XI33   A0A0N7ZA03   R7TQM1   A0A1B6E4U1   A0A0L8FZ62   D3TP22   A0A210PF26   A0A1B0C3S0   A0A1Z5LG49   A0A3S1BLC1   A0A2R5LNM6   A0A1B0A4Q3   A0A3M6TT48   A0A197JNY9   A0A1S3IB82   A0A397SG99   A0A2Z4EUI1   A0A293N471   A0A286XDX7   A0A1S4FNL7   A0A087TQT1   A0A0N8ETI2   Q16UR2   A0A2Z4EUN1   A0A0P5PKS8   A0A2Z4EUL1   T2MHR4   A0A0P5N015   A0A0P6DH27   E9GCT9   I3MPI3   A0A3M7RK75   A0A0P5SQA0   J9K7P8   B3KQM6   O95352   G7NYH8   F7ESQ7   A0A0T6B3D2   A0A0C9V069   A0A2R9C9V9   H2QM24   A0A0C9VA15   A0A023EVS1   A0A1D2N3H5   A0A2K6B8E2   A0A2I2ZVZ4   A0A0D9RBZ0   A0A2J8WDD9   B2RB57   F6TZ29   A0A1W2W0C6   A0A0P5J8H3   A0A287CXQ2   A0A250YKQ9   K7DKQ6   A0A2K5XUN0   A0A2K5MAK2   A0A2K6T6D4   A0A2K6FZ84  

PDB

4GSL     E-value=6.06266e-119,     Score=1096

KEGG

101745625    K08337 ubiquitin-like modifier-activating enzyme ATG7 | (RefSeq) ubiquitin-like modifier-activating

PATHWAY

04136    Autophagy - other - Bombyx mori (domestic silkworm)
04140    Autophagy - animal - Bombyx mori (domestic silkworm)

GO

GO:0005737   GO:0008641   GO:0006914   GO:0035096   GO:0009267   GO:0048477   GO:0006497   GO:0008340   GO:0019779   GO:0032446   GO:0016236   GO:0050688   GO:0016239   GO:0019778   GO:0000422   GO:0016567   GO:0000045   GO:1903706   GO:0006501   GO:0060548   GO:0010506   GO:0000407   GO:0043066   GO:0016021   GO:0044805   GO:0034727   GO:0006995   GO:0042802   GO:0097632   GO:0005829   GO:0039521   GO:0075044   GO:0051607   GO:0031401   GO:0008134   GO:0045732   GO:0043065   GO:0071455   GO:0042803   GO:0061025   GO:0071315   GO:0061684   GO:0043312   GO:0042752   GO:1904813   GO:0030424   GO:1902617   GO:0007568   GO:0010508   GO:0048511   GO:0005576   GO:0005930   GO:0015031   GO:1903204   GO:0006464   GO:0009749   GO:0034774   GO:0004839   GO:0090298   GO:0045071   GO:0000423   GO:0007507   GO:0006412   GO:0008270   GO:0045211   GO:0005975   GO:0004553  

PANTHER

PTHR10953:SF3  

Subcellular location

Cytoplasm   Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme.   With evidence from 4 publications.
Preautophagosomal structure   Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme.   With evidence from 4 publications.

Length:

681

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.881919999999997

Exp number, first 60 AAs:

0.04433

Total prob of N-in:

0.03369

outside

1  -  681

 

 

Pi

265.9087

Theta

184.799073

Tajima's D

1.333377

CLR

0.297095

CSRT

0.746862656867157

Interpretation

Uncertain