Gene

KWMTBOMO12481

Pre Gene Modal

BGIBMGA001472

Annotation

PREDICTED:_bumetanide-sensitive_sodium-(potassium)-chloride_cotransporter-like_[Bombyx_mori]

Location

Bomo_Chr21(-):4733263-4766662      View in EpiBrowser

Full name

Bumetanide-sensitive sodium-(potassium)-chloride cotransporter      

Alternative Name

Na-K-CL symporter

Location in the cell

PlasmaMembrane   Reliability : 4.82

CDS

ATGGAAGATAATAGATTTGTCGTTTCAACCGTGGAGGGGGAATGTAAGAAGAATGGTATCCACATGGGAGCTAATATCATATCTCGTCCACTACGGTCTTCCTTGGAGACTGTCGAGCGAGGAGTTACCAACGCGCAGCCGGACACCTGGCTCCACGATGCCGGCTGGAGGAGAAAACGCTCTCTGGCACAGCTGACAAGGGAAGCGCTCCCCAGAATGGAAAACTACAGAAATTCGAAGAGAGCCCTGAAAAGACCTAGTTTAGGTGAACTACACGGTGACCACCTAATAACTGAAGAGGAAGAGAAAACGCAGAATCCGAGAGACACAAAATCACCGACACCAGCCGGTGGAATCAAATTAGGATGGATACAAGGTGTGTTGATACCATGTCTCCTGAACATCTGGGGCGTGATGCTGTTCCTCCGTATCTCGTGGGTGGTGTCGCAGGCCGGCATTGGCTGGTCCCTGGTCATCATTGCACTATCAGCTGTTGTGTGCGTCATCACCACGCTATCTATGAGCGCAATTTGCACCAATGGAGAAGTAAAAGGAGGCGGCATTTACTACATAATATCAAGATCTCTCGGCCCAGAATTCGGGGCTTCTGTGGGCATAATATTTGCTTTTGCCAACGCCGTGGCCGCGAGCATGAACACCATCGGTTTCTGTGACTCCATGAACCACCTCCTCAAGAGCTTAGACCTGCAAATAATCGACAACAGCTACAACGACGTGAGGATCATCGGCGCCATAGCCTTATTCGTGATGTGCGTCATCTGCGCTGTCGGAATGGACTGGGAGAGTAAAGCGCAGAATTTCTTGATCGCGATTATAGTGGGCGCAATCGTCGACTTCGTTGTTGGTGCGGTCATGGGTCCGAAGAGTAACTTGGAGGTTGCAGAAGGATTTGTCGGCTTAAGTACATCAACGTTCGTAGAAAACTTCAATTCTGACTTCAAATACAGTGAGGGCATGGAGCAGAACTTCTTCAGTGTCTTCGCCATATTCTTTCCATCCGTGACCGGAATACAGGCAGGCGCGAATATATCTGGCGATCTGAAGGACCCCGCATCCGCCATACCAAAAGGCACGTTATTAGCGCTCCTAATATCTATGGTGAGCTACGCTATGATGGTTTTATTCACCGGAGCCGCAGCCCTCCGTGACGCGAGCGGAAATATCACTGACCTGGTCATCAGCAACGGCACAGTTACAAATTACAGTGCGATCATGCAACTGATGTCGGCGTGGGGCCCCTTCATCTACGCGGGCTGCTGGGCGGCGACTCTGTCCACGGCGCTCACCAACCTGCTGTCGGTGCCGCGACTCATCCAGGCGCTCGGCGTCGACCGCATCTACCCAGGACTCATCTTCTTCTCGAAACCGTACGGACGACACGGAGAAGCCTACAGAGGATACGTGCTCACCTTCTTAGTGTCCTTACTGTTTCTACTTATTGCGAAATTAAATGCGATCGCGCCATTGATCTCCAATTTCTACCTGGCATCATATGCGCTGATAAACTTTTGCACGTTTCACGCGGCGCTGGTGCGGCCTCTTGGCTGGCGACCTACATTCAAATACTACAATGTTTGGGTGTCCCTGGCCGGTTTCCTGATGTGTGTCGGGATCATGTTGCTCATCAGCTGGATCATGTCGCTCGTGACCATTGCAATATTCTTCACGCTGTATCTCATCGTGCACTACCGTAATCCCGATGTGAACTGGGGCAGTAGCACGCAAGCTCAGATGTACAAGACGGCGTTGTCCAGTGCCCACAACCTGGCCCGCACTGGGGAGCACGTGAAGAACTACTGGCCGCAACTGCTGGTGCTGGGGGGACGCGCCCACGCTCGTCCGCCGCTCGTAGACCTGGGTAGCCTCATCACCAAAGCCGGCTCTCTTATGATCATTGGAGATATTTCGAAGGAAAAACTGTCATACAAGGTGTGCTCAGCGCGAGCTCGTGCAGATAACGAATGGCTGCAAGAGAGGAAAGTGCGCGCCTTCTGTTCGCTAGTGCACGGATTCAACTTTGAACAAGGAGCACGAGCCCTGATACAGGCGACCGGCGTCGGCAAACTGGCGCCCAACGTGTTACTCATGGGCTACAAGTCAGATTGGACAACAGCTTCGGCCGAAGACCTCGTTGCTTATTTCAATGTGTTACACACGGCGTTCGAGAACCGTCTGGCCGTCGCTATAGTCCGTGTGCGCGGCGGGCTGGACTACGGCGCGGCGGGGGGTGGGGAAGAGGGCGCGGGGTCGCTGACCGTCACGTCGAGCGGTTCGGGCGAGCTACACGTGCGCCGCTCAGACGCGCTCATCATGCACGCGGACTCAGACCTCGACATCCACACCGACTCCTCCGCCAAGAACAATCTTTCCAATATTTTAACACTGTCCACTTCGCGATCGTTCACAATAACTGATGCCAAAGACATAAAAGATAAGAAAAAGAAAGACAAAAGGCCCACCGACATGCATCGTCAGATCATCTACAACGCGGCGAACGGAATTGAACTGTCTAAAGATCAATTAACTCAGATGTCAATATTTAAACGGAAACAGGAATCCGGGACGGTGGACGTGTGGTGGCTATACGACGACGGCGGCCTCACAATCCTGCTCCCGTACATCATCTCGCAACGATCCGCCTGGGGGAACTGCAAACTTCGTATATTCGCACTCGCTAACCGACTCAACGAAATGGAGCTCGAAGAAAGAAATATGGCCAATTTACTGTCGAAGTTCCGGATTGACTATTCGTCGTTGACAATGGTCCAAGACATAACGGAACCTCCGCAAGCAGAGACCAAAGCGCTCTTCGATGAGACCATAAAGAAATTTACAAGTGATTCTGCTGCCCCAGAGTGTCGTATTAGCGAGACGGAGTTGACGACGCTATCCGGTAAGACGAATAGGCAGCTGCGACTGAGGGAGCTTTTACTCGCCAACTCAAGGGACTCGAGGCTCATCGTTATGTCGCTACCCATGCCCAGAAAGGGCTCCGTGTCAGCGCCGCTGTACATGGCGTGGCTAGAGATGATGAGTCGTGACCTGCCGCCGATGCTCTTCGTACGAGGGAACCACACTTCAGTGCTCACGTTCTATTCTTGA

Protein

MEDNRFVVSTVEGECKKNGIHMGANIISRPLRSSLETVERGVTNAQPDTWLHDAGWRRKRSLAQLTREALPRMENYRNSKRALKRPSLGELHGDHLITEEEEKTQNPRDTKSPTPAGGIKLGWIQGVLIPCLLNIWGVMLFLRISWVVSQAGIGWSLVIIALSAVVCVITTLSMSAICTNGEVKGGGIYYIISRSLGPEFGASVGIIFAFANAVAASMNTIGFCDSMNHLLKSLDLQIIDNSYNDVRIIGAIALFVMCVICAVGMDWESKAQNFLIAIIVGAIVDFVVGAVMGPKSNLEVAEGFVGLSTSTFVENFNSDFKYSEGMEQNFFSVFAIFFPSVTGIQAGANISGDLKDPASAIPKGTLLALLISMVSYAMMVLFTGAAALRDASGNITDLVISNGTVTNYSAIMQLMSAWGPFIYAGCWAATLSTALTNLLSVPRLIQALGVDRIYPGLIFFSKPYGRHGEAYRGYVLTFLVSLLFLLIAKLNAIAPLISNFYLASYALINFCTFHAALVRPLGWRPTFKYYNVWVSLAGFLMCVGIMLLISWIMSLVTIAIFFTLYLIVHYRNPDVNWGSSTQAQMYKTALSSAHNLARTGEHVKNYWPQLLVLGGRAHARPPLVDLGSLITKAGSLMIIGDISKEKLSYKVCSARARADNEWLQERKVRAFCSLVHGFNFEQGARALIQATGVGKLAPNVLLMGYKSDWTTASAEDLVAYFNVLHTAFENRLAVAIVRVRGGLDYGAAGGGEEGAGSLTVTSSGSGELHVRRSDALIMHADSDLDIHTDSSAKNNLSNILTLSTSRSFTITDAKDIKDKKKKDKRPTDMHRQIIYNAANGIELSKDQLTQMSIFKRKQESGTVDVWWLYDDGGLTILLPYIISQRSAWGNCKLRIFALANRLNEMELEERNMANLLSKFRIDYSSLTMVQDITEPPQAETKALFDETIKKFTSDSAAPECRISETELTTLSGKTNRQLRLRELLLANSRDSRLIVMSLPMPRKGSVSAPLYMAWLEMMSRDLPPMLFVRGNHTSVLTFYS

Catalytic Activity

H2O + O-phospho-L-tyrosyl-[protein] = L-tyrosyl-[protein] + phosphate

Similarity

Belongs to the SLC12A transporter family.

Keywords

Chloride   Glycoprotein   Ion transport   Membrane   Potassium   Potassium transport   Sodium   Sodium transport   Symport   Transmembrane   Transmembrane helix   Transport  

Feature

chain  Bumetanide-sensitive sodium-(potassium)-chloride cotransporter

Uniprot

H9IW41   A0A097A1K3   Q25479   A0A2A4JL60   A0A212ETB9   A0A194Q284   + More
A0A437BI80   A0A0N1IFG1   A0A1Y1N8X4   A0A1B0EWP1   A0A139WN29   A0A1B6EM82   A0A2A4JMK0   A0A1B6JQ16   N6TV98   U4U4I0   A0A1L8DDI6   A0A1L8DDK5   A0A0P4VIR1   E0VSF3   A0A224XAK8   A0A336LTI1   A0A1Q3FSA3   A0A023EWB6   Q16UI3   A0A1S4FNN9   A0A0L7R0A0   A0A182G664   A0A0A9YLQ8   A0A146L8H8   B0WKT5   A0A067QMC3   E1ZW64   A0A232F830   A0A087ZNH9   B4JHV1   A0A0M8ZS04   A0A1W4UJR9   B4KD91   B4LZB6   A0A0C9PJL4   B3LZ40   A0A154PSG8   B3P2E4   Q9VNC7   A0A0M5IZZ9   Q296F6   B4PVA9   A0A1I8P469   B4NL26   B4GF84   A0A151INH0   A0A1W4UYF5   E2BVQ8   A0A2A3E799   A0A151XGF5   A0A1A9Z8G9   A0A182JS22   A0A182VQN7   Q9VNC6   A0A195DV50   W8BU02   A0A1A9VB44   A0A026W772   A0A1A9YIQ2   A0A1Y0AWN1   A0A034VJ51   A0A1J1ID56   A0A1L8EG66   A0A1L8EBT6   A0A1L8EG76   A0A1L8EB04   A0A0K8UVZ0   A0A1I8M5P1   T1PDT6   A0A3B0JTJ3   A0A182JN28   A0A158ND36   A0A2C9GV90   A0A151I295   A0A1A9X1V7   A0A182M535   A0A1L8EBF9   A0A195F7T2   A0A0L0CL19   Q16UI2   A0A1S4FNU9   A0A182YK80   B4I413   A0A084W3D0   A0A0P9C794   A0A182Q954   Q5TXE0   A0A182LCP0   A0A182PIC3   A0A2M3ZF49   A0A2M3YXG8   A0A2M3ZF84   B0WKT4  

Pubmed

19121390    7550244    22118469    26354079    28004739    18362917    + More
19820115    23537049    27129103    20566863    24945155    17510324    26483478    25401762    26823975    24845553    20798317    28648823    17994087    10731132    12537568    12537572    12537573    12537574    16110336    17569856    17569867    26109357    26109356    15632085    17550304    24495485    24508170    28341416    25348373    25315136    21347285    26108605    25244985    24438588    12364791    14747013    17210077    20966253   

EMBL

BABH01005908    BABH01005909    BABH01005910    BABH01005911    BABH01005912    BABH01005913    + More
KM111607    AIS39469.1    U17344    NWSH01001068    PCG72815.1    AGBW02012606    OWR44727.1    KQ459564    KPI99651.1    RSAL01000055    RVE49975.1    KQ461068    KPJ09892.1    GEZM01009755    JAV94333.1    AJWK01006428    KQ971312    KYB29205.1    GECZ01030739    GECZ01015000    JAS39030.1    JAS54769.1    PCG72814.1    GECU01006426    JAT01281.1    APGK01010025    APGK01010026    APGK01010027    APGK01053218    APGK01053219    APGK01053220    KB741223    KB738096    ENN72321.1    ENN82770.1    KB630198    KB631707    ERL83470.1    ERL85501.1    GFDF01009551    JAV04533.1    GFDF01009552    JAV04532.1    GDKW01001818    JAI54777.1    DS235750    EEB16309.1    GFTR01008462    JAW07964.1    UFQT01000100    SSX19929.1    GFDL01004689    JAV30356.1    GAPW01000083    JAC13515.1    CH477621    EAT38198.1    KQ414671    KOC64262.1    JXUM01000651    JXUM01000652    JXUM01000653    JXUM01000654    JXUM01000655    JXUM01000656    JXUM01000657    JXUM01000658    JXUM01000659    JXUM01000660    KQ560109    KXJ84476.1    GBHO01013124    JAG30480.1    GDHC01015659    JAQ02970.1    DS231976    EDS30058.1    KK853171    KDR10319.1    GL434776    EFN74573.1    NNAY01000772    OXU26603.1    CH916369    EDV92859.1    KQ435936    KOX68349.1    CH933806    EDW14873.1    CH940650    EDW68151.1    GBYB01001143    JAG70910.1    CH902617    EDV41914.1    KQ435131    KZC14865.1    CH954181    EDV47894.1    AE014297    AY122102    AAF52017.2    AAM52614.1    CP012526    ALC46644.1    CM000070    EAL28501.1    CM000160    EDW95778.1    CH964272    EDW84229.1    CH479182    EDW34269.1    KQ976933    KYN07006.1    GL450951    EFN80216.1    KZ288358    PBC27049.1    KQ982173    KYQ59378.1    AAF52018.2    KQ980341    KYN16434.1    GAMC01013964    GAMC01013961    JAB92594.1    KK107364    EZA51905.1    KY921816    ART29405.1    GAKP01016429    GAKP01016423    JAC42529.1    CVRI01000047    CRK98147.1    GFDG01001165    JAV17634.1    GFDG01002723    JAV16076.1    GFDG01001155    JAV17644.1    GFDG01002849    JAV15950.1    GDHF01026214    GDHF01021482    GDHF01008314    JAI26100.1    JAI30832.1    JAI44000.1    KA646937    AFP61566.1    OUUW01000008    SPP84373.1    ADTU01012293    ADTU01012294    KQ976547    KYM80928.1    AXCM01001650    GFDG01002850    JAV15949.1    KQ981744    KYN36441.1    JRES01000249    KNC32985.1    EAT38199.1    CH480821    EDW54956.1    ATLV01019917    ATLV01019918    KE525285    KFB44724.1    KPU79431.1    AXCN02000174    AAAB01008794    EAL42059.4    GGFM01006391    MBW27142.1    GGFM01000175    MBW20926.1    GGFM01006394    MBW27145.1    EDS30057.1   

Proteomes

UP000005204    UP000218220    UP000007151    UP000053268    UP000283053    UP000053240    + More
UP000092461    UP000007266    UP000019118    UP000030742    UP000009046    UP000008820    UP000053825    UP000069940    UP000249989    UP000002320    UP000027135    UP000000311    UP000215335    UP000005203    UP000001070    UP000053105    UP000192221    UP000009192    UP000008792    UP000007801    UP000076502    UP000008711    UP000000803    UP000092553    UP000001819    UP000002282    UP000095300    UP000007798    UP000008744    UP000078542    UP000008237    UP000242457    UP000075809    UP000092445    UP000075881    UP000075920    UP000078492    UP000078200    UP000053097    UP000092443    UP000183832    UP000095301    UP000268350    UP000075880    UP000005205    UP000075884    UP000078540    UP000091820    UP000075883    UP000078541    UP000037069    UP000076408    UP000001292    UP000030765    UP000075886    UP000007062    UP000075882    UP000075885   

PRIDE

H9IW41    Q25479    Q9VNC7    Q9VNC6   

Pfam

PF00324   AA_permease        + More
PF03522   SLC12
PF00102   Y_phosphatase
PF08403   AA_permease_N

Interpro

IPR004842   SLC12A_fam        + More
IPR004841   AA-permease/SLC12A_dom       
IPR018491   SLC12_C       
IPR002443   SLC12A1/SLC12A2       
IPR016130   Tyr_Pase_AS       
IPR003595   Tyr_Pase_cat       
IPR029021   Prot-tyrosine_phosphatase-like       
IPR000242   PTPase_domain       
IPR020846   MFS_dom       
IPR000387   TYR_PHOSPHATASE_dom       
IPR013612   AA_permease_N       
IPR023298   ATPase_P-typ_TM_dom_sf       

SUPFAM

SSF52799   SSF52799        + More
SSF81665   SSF81665       

Gene 3D

3.90.190.10    

CDD

cd06174  MFS

ProteinModelPortal

H9IW41   A0A097A1K3   Q25479   A0A2A4JL60   A0A212ETB9   A0A194Q284   + More
A0A437BI80   A0A0N1IFG1   A0A1Y1N8X4   A0A1B0EWP1   A0A139WN29   A0A1B6EM82   A0A2A4JMK0   A0A1B6JQ16   N6TV98   U4U4I0   A0A1L8DDI6   A0A1L8DDK5   A0A0P4VIR1   E0VSF3   A0A224XAK8   A0A336LTI1   A0A1Q3FSA3   A0A023EWB6   Q16UI3   A0A1S4FNN9   A0A0L7R0A0   A0A182G664   A0A0A9YLQ8   A0A146L8H8   B0WKT5   A0A067QMC3   E1ZW64   A0A232F830   A0A087ZNH9   B4JHV1   A0A0M8ZS04   A0A1W4UJR9   B4KD91   B4LZB6   A0A0C9PJL4   B3LZ40   A0A154PSG8   B3P2E4   Q9VNC7   A0A0M5IZZ9   Q296F6   B4PVA9   A0A1I8P469   B4NL26   B4GF84   A0A151INH0   A0A1W4UYF5   E2BVQ8   A0A2A3E799   A0A151XGF5   A0A1A9Z8G9   A0A182JS22   A0A182VQN7   Q9VNC6   A0A195DV50   W8BU02   A0A1A9VB44   A0A026W772   A0A1A9YIQ2   A0A1Y0AWN1   A0A034VJ51   A0A1J1ID56   A0A1L8EG66   A0A1L8EBT6   A0A1L8EG76   A0A1L8EB04   A0A0K8UVZ0   A0A1I8M5P1   T1PDT6   A0A3B0JTJ3   A0A182JN28   A0A158ND36   A0A2C9GV90   A0A151I295   A0A1A9X1V7   A0A182M535   A0A1L8EBF9   A0A195F7T2   A0A0L0CL19   Q16UI2   A0A1S4FNU9   A0A182YK80   B4I413   A0A084W3D0   A0A0P9C794   A0A182Q954   Q5TXE0   A0A182LCP0   A0A182PIC3   A0A2M3ZF49   A0A2M3YXG8   A0A2M3ZF84   B0WKT4  

KEGG

101744951    K10951 solute carrier family 12 (sodium/potassium/chloride transporter), member 2 | (RefSeq) bumetan

GO

GO:0015377   GO:0016021   GO:0006814   GO:0006813   GO:1990573   GO:0055075   GO:0006884   GO:0008511   GO:0055064   GO:0015081   GO:0015378   GO:1902476   GO:0004725   GO:0035725   GO:0015379   GO:0055078   GO:0005623   GO:0005215   GO:0006811   GO:0055085   GO:0003333   GO:0015171   GO:0016020   GO:0006810   GO:0045211   GO:0005975   GO:0004553  

Subcellular location

Membrane  

Length:

1040

Number of predicted TMHs:

11

Exp number of AAs in TMHs:

247.3163

Exp number, first 60 AAs:

0

Total prob of N-in:

0.87625

inside

1  -  120

TMhelix

121  -  142

outside

143  -  156

TMhelix

157  -  179

inside

180  -  199

TMhelix

200  -  222

outside

223  -  247

TMhelix

248  -  267

inside

268  -  273

TMhelix

274  -  293

outside

294  -  329

TMhelix

330  -  352

inside

353  -  364

TMhelix

365  -  387

outside

388  -  416

TMhelix

417  -  439

inside

440  -  474

TMhelix

475  -  497

outside

498  -  500

TMhelix

501  -  523

inside

524  -  529

TMhelix

530  -  552

outside

553  -  1040

 

 

Pi

224.916833

Theta

165.54689

Tajima's D

1.180486

CLR

0.291198

CSRT

0.703614819259037

Interpretation

Uncertain