Gene

KWMTBOMO12355

Pre Gene Modal

BGIBMGA012391

Annotation

PREDICTED:_chondroitin_sulfate_synthase_1_[Bombyx_mori]

Location

Bomo_Chr21(+):1007317-1010113      View in EpiBrowser

Full name

Hexosyltransferase       + More
Chondroitin sulfate synthase 1      

Alternative Name

Chondroitin glucuronyltransferase 1
Chondroitin synthase 1
Glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase 1
N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase 1
N-acetylgalactosaminyltransferase 1

Location in the cell

Cytoplasmic   Reliability : 3.141

CDS

ATGGAGATTATTAATGCACCGTCTCGTCAACGAGGACGTGTGATTGACTTCAACGAGCTTCTGTACGGATACACCAGGCTCCAGCCGGTGCACGGGGCCGATCACATCTTGGATCTGCTGTTGAAGTACAGGAGATACAGAGGAAGGAAAATGACAGCGGCAGTTAGACGACATGCTTATTTGCAGCAGTCATTTACAGGTATGGAGATAAGAGAGTTACCAATGGGAGATCCGCCTCCATTTGAAGATCCAGCCATACTAGAAAAGACTGAGAATGGATTATATCCGAACGACTACGAGGACTTTGACGAGTCCAGTGCTCAACAGAGCAGCTTTTTGGACTTCGGTAAATTAAAAGTCAAGGAAGCTCTCGAAACTGGTCTTCAGAAGATCCAGAACAACTTACCCAAGGTGTTGAAGTGGAATAGTGATGATTCTGAAGAGTACCCGGTGTATGACAGAAAAATACACTTCGTTCTGCCCCTATCTGGTAGACAAGAGACATTCGGTAGATTCATGAAGAACTACGAAGATATAGTTTTAAAAAGCAGTCAAGCTGCCTCTCTAATTGTCGTAGCTTATTTAGATAGCAAACATCCATTGGATTATAGAAACACAGAGTTACTAATTAATTATTATATCGAGATGTACGGGAAAGACATCCAAATAGTTAAAATGGGGTCGAATATGTTTTCACGGGGAGCCGCCTTGACCGAGGGACTCAAGCTCTGCGATAGCGACGACCTTGTCTTCTTCATTGACGTGGACATGATGTTCAACCACGAGAGCCTTCGTCGAATAAGAATCAACACAATAAAATATAACCAAGTTTATTTCCCAATAGTGTTTAGTGAGTACAATCCAGATGTAGTGAACGGTGATGATTATAATAAAGTTAAAGGCGAAACTCTGTTTGTGGACACTGAATTGGAGAAGTTTATTGATGACGAAGAAACAGTGAGAACGGACAGAGAATTGGCGAATTTGAAGTACAGCCGGGAAATAACTGATGATACGGGCTATTTCAGGCAGTACGGTTTTGGTATTTTAGCTATTTATAAACGTGACTTCGAACGGATAGGGGGATTCGATTTGAACATTAAGGGTTGGGGTCTAGAAGACGTGCAGCTGTTTGAAACGCTAATTAAATCAAATTTAACGGTATACAGGATAGCTGACGAGACTCTAGTCCATATATTCCATTCGGTTGACTGTGATAAAAATTTAGAGAAGAGCCAGTTCAAGATGTGTCTGGGAACGAAGGCATCGACTTACGGCAGTGAAAAGCACATGGCGTATTATATGCTAAACCACCCTGATGTCCTGTGGCCGCCACAAGATAAACCGGAAGCGGAAGCGGAAGTGGTGGAAGAGAAACCTAAGGAAAAGAACGAGAAAGGTGCCAGGTAG

Protein

MEIINAPSRQRGRVIDFNELLYGYTRLQPVHGADHILDLLLKYRRYRGRKMTAAVRRHAYLQQSFTGMEIRELPMGDPPPFEDPAILEKTENGLYPNDYEDFDESSAQQSSFLDFGKLKVKEALETGLQKIQNNLPKVLKWNSDDSEEYPVYDRKIHFVLPLSGRQETFGRFMKNYEDIVLKSSQAASLIVVAYLDSKHPLDYRNTELLINYYIEMYGKDIQIVKMGSNMFSRGAALTEGLKLCDSDDLVFFIDVDMMFNHESLRRIRINTIKYNQVYFPIVFSEYNPDVVNGDDYNKVKGETLFVDTELEKFIDDEETVRTDRELANLKYSREITDDTGYFRQYGFGILAIYKRDFERIGGFDLNIKGWGLEDVQLFETLIKSNLTVYRIADETLVHIFHSVDCDKNLEKSQFKMCLGTKASTYGSEKHMAYYMLNHPDVLWPPQDKPEAEAEVVEEKPKEKNEKGAR

Description

Has both beta-1,3-glucuronic acid and beta-1,4-N-acetylgalactosamine transferase activity. Transfers glucuronic acid (GlcUA) from UDP-GlcUA and N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of the elongating chondroitin polymer.
Has both beta-1,3-glucuronic acid and beta-1,4-N-acetylgalactosamine transferase activity. Transfers glucuronic acid (GlcUA) from UDP-GlcUA and N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of the elongating chondroitin polymer. Involved in the negative control of osteogenesis likely through the modulation of NOTCH signaling.

Catalytic Activity

3-O-(beta-D-GlcA-(1->3)-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-seryl-[protein] + UDP-N-acetyl-alpha-D-galactosamine = 3-O-(beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-seryl-[protein] + H(+) + UDP
3-O-(beta-D-GlcA-(1->3)-[beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)](n)-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-seryl-[protein] + UDP-N-acetyl-alpha-D-galactosamine = 3-O-([beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)](n+1)-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-seryl-[protein] + H(+) + UDP
3-O-(beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-seryl-[protein] + UDP-alpha-D-glucuronate = 3-O-(beta-D-GlcA-(1->3)-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-seryl-[protein] + H(+) + UDP
3-O-([beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)](n)-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-seryl-[protein] + UDP-alpha-D-glucuronate = 3-O-(beta-D-GlcA-(1->3)-[beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)](n)-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-seryl-[protein] + H(+) + UDP

Subunit

Binds CHPF.

Similarity

Belongs to the chondroitin N-acetylgalactosaminyltransferase family.

Keywords

Complete proteome   Glycoprotein   Golgi apparatus   Membrane   Metal-binding   Reference proteome   Secreted   Signal-anchor   Transferase   Transmembrane   Transmembrane helix   Disease mutation   Polymorphism  

Feature

chain  Hexosyltransferase
sequence variant  In TPBS.

Uniprot

H9JS81   A0A1E1W9F5   A0A0L7LT67   A0A3S2NLT0   A0A2A4JUH5   A0A2W1BGU7   + More
A0A194RA34   A0A2H1X317   A0A212F2Z6   A0A194PUH9   A0A2J7Q327   A0A2R7VP29   A0A067QLV6   A0A1B6K3F6   T1HKH9   A0A1Y1K292   A0A2P8Y5F4   A0A154PEY4   F4WTX6   A0A2J7Q328   A0A3M7PVV5   A0A0T6B2E9   A0A151JUT0   A0A151WW89   A0A3L8E3L7   A0A026VXM4   A0A158P0Y9   A0A087ZN64   A0A1S3JT82   U4UJU7   A0A0P4WQS3   A0A195AUR8   A0A151J1R6   A0A151ILV9   G3STR9   A0A3P4M7Q2   E0VDG3   A0A2Y9QQD1   V4B4G2   B0X8P8   N6U1C6   E2C9U3   A0A182H1K5   Q6ZQ11   B4DLD0   Q175Z7   A0A182KC49   A0A1S4FDT8   S9YF80   A0A0K8S8T2   H9F9J0   A0A1W4WDF8   A0A383ZW95   A0A212CSS3   L8IAE5   A0A340W9T5   A0A2K5F8I5   H2NPC0   E1ZW55   F7ALM7   A0A182MEV0   A0A1B6EH51   A0A2R9C2N3   A0A2J8VXU2   A0A2Y9GDN9   A0A2K6TUG0   A0A2K5F8K8   G3VMU5   A0A2R9C262   A0A2K6EVH1   K7C1E9   K7D3N2   A0A2I3H8Z7   Q86X52   A0A210QB45   A0A2K5RAV2   F7HY89   A0A383ZVN5   G3R0R0   W5PI31   A0A2I3GNC5   A0A1D5RD54   A0A1U7U9G8   F1SR91   A0A2K5ICG6   A0A2U4C7Z0   A0A0D9RWB0   A0A2K6R1Q3   A0A287BB21   A0A2K5YES0   A0A2Y9QGI2   K1R4W5   A0A2K6LE64   A0A2K6AQ72   A0A2K6R1L7   A0A287AWP7   A0A3Q7T9R5   G7MWB6   H0WNJ9   A0A341D052  

EC Number

2.4.1.-

Pubmed

19121390    26227816    28756777    26354079    22118469    24845553    + More
28004739    29403074    21719571    30375419    30249741    24508170    21347285    23537049    20566863    23254933    20798317    26483478    14621295    17510324    23149746    26823975    25319552    22751099    17495919    22722832    21709235    11514575    10231032    12975309    15489334    12716890    12907687    21129727    25944712    21129728    28812685    25243066    22398555    20809919    17431167    25362486    22992520    30723633    22002653   

EMBL

BABH01033107    BABH01033108    BABH01033109    BABH01033110    BABH01033111    BABH01033112    + More
BABH01033113    GDQN01007563    JAT83491.1    JTDY01000138    KOB78645.1    RSAL01000284    RVE42988.1    NWSH01000557    PCG75675.1    KZ150229    PZC72020.1    KQ460473    KPJ14487.1    ODYU01013060    SOQ59721.1    AGBW02010645    OWR48109.1    KQ459591    KPI97061.1    NEVH01019070    PNF22983.1    KK854011    PTY09284.1    KK853171    KDR10328.1    GECU01001731    JAT05976.1    ACPB03002001    ACPB03002002    GEZM01096747    JAV54588.1    PYGN01000906    PSN39485.1    KQ434890    KZC10413.1    GL888345    EGI62333.1    PNF22984.1    REGN01008568    RNA03237.1    LJIG01016119    KRT81558.1    KQ981732    KYN36557.1    KQ982691    KYQ52153.1    QOIP01000001    RLU27340.1    KK107602    EZA48558.1    ADTU01005907    ADTU01005908    ADTU01005909    KB632237    ERL90461.1    GDRN01070254    JAI63913.1    KQ976738    KYM75765.1    KQ980514    KYN15733.1    KQ977086    KYN05859.1    CYRY02007166    VCW76315.1    DS235075    EEB11419.1    KB199650    ESP05353.1    DS232496    EDS42640.1    APGK01043593    APGK01043594    KB741015    ENN75350.1    GL453902    EFN75280.1    JXUM01023219    KQ560655    KXJ81415.1    AK129255    AK296943    BAG59492.1    CH477391    EAT41961.1    KB016893    EPY82795.1    GBRD01016277    GDHC01021462    GDHC01014811    JAG49549.1    JAP97166.1    JAQ03818.1    JU327543    AFE71299.1    MKHE01000013    OWK09057.1    JH881576    ELR53460.1    ABGA01296818    ABGA01296819    ABGA01296820    ABGA01296821    ABGA01296822    GL434776    EFN74564.1    AXCM01001757    GEDC01000030    JAS37268.1    AJFE02100904    AJFE02100905    AJFE02100906    NDHI03003407    PNJ62351.1    AEFK01007197    AEFK01007198    AEFK01007199    AEFK01007200    AEFK01007201    AEFK01007202    AEFK01007203    GABC01005910    GABC01005909    GABC01005908    GABC01005907    GABC01005906    GABC01005905    GABC01005904    GABF01004070    NBAG03000304    JAA05428.1    JAA18075.1    PNI43251.1    GABD01003944    GABD01003943    GABD01003942    GABD01003941    GABD01003940    GABE01010434    GABE01010433    GABE01010432    GABE01010431    GABE01010430    JAA29156.1    JAA34309.1    ADFV01131171    ADFV01131172    ADFV01131173    ADFV01131174    ADFV01131175    ADFV01131176    ADFV01131177    ADFV01131178    ADFV01131179    AB071402    AB023207    AY358529    BC046247    NEDP02004357    OWF45955.1    GAMT01009289    GAMT01009288    GAMP01007101    JAB02572.1    JAB45654.1    CABD030098189    CABD030098190    CABD030098191    AMGL01040772    AMGL01040773    AMGL01040774    JSUE03039225    JSUE03039226    AEMK02000004    AQIB01128694    AQIB01128695    JH817566    EKC36260.1    DQIR01115652    DQIR01199911    HDA71128.1    CM001259    EHH27640.1    AAQR03057961    AAQR03057962    AAQR03057963    AAQR03057964   

Proteomes

UP000005204    UP000037510    UP000283053    UP000218220    UP000053240    UP000007151    + More
UP000053268    UP000235965    UP000027135    UP000015103    UP000245037    UP000076502    UP000007755    UP000276133    UP000078541    UP000075809    UP000279307    UP000053097    UP000005205    UP000005203    UP000085678    UP000030742    UP000078540    UP000078492    UP000078542    UP000007646    UP000009046    UP000248480    UP000030746    UP000002320    UP000019118    UP000008237    UP000069940    UP000249989    UP000000589    UP000008820    UP000075881    UP000192223    UP000261681    UP000265300    UP000233020    UP000001595    UP000000311    UP000002280    UP000075883    UP000240080    UP000248481    UP000233220    UP000007648    UP000233160    UP000001073    UP000005640    UP000242188    UP000233040    UP000008225    UP000001519    UP000002356    UP000006718    UP000189704    UP000008227    UP000233080    UP000245320    UP000029965    UP000233200    UP000233140    UP000248483    UP000005408    UP000233180    UP000233120    UP000286640    UP000005225    UP000252040   

PRIDE

H9JS81    Q6ZQ11    B4DLD0    Q86X52    F1SR91   

Pfam

PF05679   CHGN        + More
PF02434   Fringe
PF00059   Lectin_C
PF00082   Peptidase_S8
PF01483   P_proprotein
PF06936   Selenoprotein_S
PF14843   GF_recep_IV
PF08686   PLAC

Interpro

IPR029044   Nucleotide-diphossugar_trans        + More
IPR008428   Chond_GalNAc       
IPR003378   Fringe-like       
IPR016186   C-type_lectin-like/link_sf       
IPR001304   C-type_lectin-like       
IPR018378   C-type_lectin_CS       
IPR016187   CTDL_fold       
IPR032675   LRR_dom_sf       
IPR034182   Kexin/furin       
IPR023828   Peptidase_S8_Ser-AS       
IPR002884   P_dom       
IPR009703   Selenoprotein_S       
IPR009030   Growth_fac_rcpt_cys_sf       
IPR008979   Galactose-bd-like_sf       
IPR036852   Peptidase_S8/S53_dom_sf       
IPR006212   Furin_repeat       
IPR010909   PLAC       
IPR032778   GF_recep_IV       
IPR000209   Peptidase_S8/S53_dom       

SUPFAM

SSF53448   SSF53448        + More
SSF56436   SSF56436       
SSF57184   SSF57184       
SSF49785   SSF49785       
SSF52743   SSF52743       

Gene 3D

3.90.550.10     3.10.100.10     2.60.120.260     3.40.50.200     3.80.10.10    

CDD

cd00064  FU
cd04059  Peptidases_S8_Protein_converta

ProteinModelPortal

H9JS81   A0A1E1W9F5   A0A0L7LT67   A0A3S2NLT0   A0A2A4JUH5   A0A2W1BGU7   + More
A0A194RA34   A0A2H1X317   A0A212F2Z6   A0A194PUH9   A0A2J7Q327   A0A2R7VP29   A0A067QLV6   A0A1B6K3F6   T1HKH9   A0A1Y1K292   A0A2P8Y5F4   A0A154PEY4   F4WTX6   A0A2J7Q328   A0A3M7PVV5   A0A0T6B2E9   A0A151JUT0   A0A151WW89   A0A3L8E3L7   A0A026VXM4   A0A158P0Y9   A0A087ZN64   A0A1S3JT82   U4UJU7   A0A0P4WQS3   A0A195AUR8   A0A151J1R6   A0A151ILV9   G3STR9   A0A3P4M7Q2   E0VDG3   A0A2Y9QQD1   V4B4G2   B0X8P8   N6U1C6   E2C9U3   A0A182H1K5   Q6ZQ11   B4DLD0   Q175Z7   A0A182KC49   A0A1S4FDT8   S9YF80   A0A0K8S8T2   H9F9J0   A0A1W4WDF8   A0A383ZW95   A0A212CSS3   L8IAE5   A0A340W9T5   A0A2K5F8I5   H2NPC0   E1ZW55   F7ALM7   A0A182MEV0   A0A1B6EH51   A0A2R9C2N3   A0A2J8VXU2   A0A2Y9GDN9   A0A2K6TUG0   A0A2K5F8K8   G3VMU5   A0A2R9C262   A0A2K6EVH1   K7C1E9   K7D3N2   A0A2I3H8Z7   Q86X52   A0A210QB45   A0A2K5RAV2   F7HY89   A0A383ZVN5   G3R0R0   W5PI31   A0A2I3GNC5   A0A1D5RD54   A0A1U7U9G8   F1SR91   A0A2K5ICG6   A0A2U4C7Z0   A0A0D9RWB0   A0A2K6R1Q3   A0A287BB21   A0A2K5YES0   A0A2Y9QGI2   K1R4W5   A0A2K6LE64   A0A2K6AQ72   A0A2K6R1L7   A0A287AWP7   A0A3Q7T9R5   G7MWB6   H0WNJ9   A0A341D052  

PDB

4EEO     E-value=0.0219878,     Score=89

KEGG

101736840    K13499 chondroitin sulfate synthase [EC:2.4.1.175 2.4.1.226] | (RefSeq) chondroitin sulfate synthase

PATHWAY

00532    Glycosaminoglycan biosynthesis - chondroitin sulfate / dermatan sulfate - Bombyx mori (domestic silk
01100    Metabolic pathways - Bombyx mori (domestic silkworm)

GO

GO:0032580   GO:0008376   GO:0016021   GO:0016740   GO:0060349   GO:0005576   GO:0030206   GO:0051923   GO:0047238   GO:0030279   GO:0009954   GO:0002063   GO:0045880   GO:0030246   GO:0050510   GO:0016757   GO:0051216   GO:0031667   GO:0046872   GO:0006886   GO:0004252   GO:0030176   GO:0000139   GO:0016020   GO:0008270   GO:0005509   GO:0005524   GO:0003909   GO:0051103   GO:0000049   GO:0005975   GO:0004553  

Subcellular location

  
Golgi apparatus  
Golgi stack membrane  
Secreted  

Length:

469

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.00702999999999999

Exp number, first 60 AAs:

0.00354

Total prob of N-in:

0.00578

outside

1  -  469

 

 

Pi

71.541114

Theta

141.692279

Tajima's D

-1.362199

CLR

415.174814

CSRT

0.0788460576971151

Interpretation

Uncertain