Pre Gene Modal
BGIBMGA009182
Annotation
putative_NADH_dehydrogenase_[Danaus_plexippus]
Full name
Queuine tRNA-ribosyltransferase catalytic subunit 1
Alternative Name
Guanine insertion enzyme
tRNA-guanine transglycosylase
tRNA-guanine transglycosylase
Location in the cell
Mitochondrial Reliability : 2.457
Sequence
CDS
ATGGGTGGTCACGGACATGGGCCCCCGTACACAGTGCCTCACTACTCCCAGTTTACTGTCAAAGGTATCCCACAACTGGATGAGCTTGAAAAGGCCTTAGCTGTTAAAGGACTTAAAGATCCATGGATCAGAAATGAGGCTTGGAGATATCACCCTGGATTTGGCACTCGCTGGCAGAGAGCCAGAAAATTCTTCTTTAGAGGATTTCCCATTGGACTGGGACTCACAGTAATCACAGTGGCTCTGGATAAATTTATGGGAGGGGGTGAACATGGGCATGGTCACGGGCACGAGGGAGAGCATTGA
Protein
MGGHGHGPPYTVPHYSQFTVKGIPQLDELEKALAVKGLKDPWIRNEAWRYHPGFGTRWQRARKFFFRGFPIGLGLTVITVALDKFMGGGEHGHGHGHEGEH
Summary
Description
Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product.
Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form the product. After dissociation, two additional enzymatic reactions on the tRNA convert PreQ1 to queuine (Q), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).
Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form the product. After dissociation, two additional enzymatic reactions on the tRNA convert PreQ1 to queuine (Q), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).
Catalytic Activity
7-aminomethyl-7-carbaguanine + guanosine(34) in tRNA = 7-aminomethyl-7-carbaguanosine(34) in tRNA + guanine
guanosine(34) in tRNA + queuine = guanine + queuosine(34) in tRNA
guanosine(34) in tRNA + queuine = guanine + queuosine(34) in tRNA
Cofactor
Zn(2+)
Subunit
Heterodimer of a catalytic subunit and an accessory subunit.
Similarity
Belongs to the queuine tRNA-ribosyltransferase family.
Uniprot
H9JI34
A0A437AUS2
A0A212FIG3
S4PGR4
A0A0L7KWG6
A0A2A4IZY4
+ More
A0A0N1IPA4 A0A2W1BAY6 A0A0N1PFF7 I4DJV3 A0A1E1WKQ2 A0A0L0BPF2 A0A023ECZ1 Q173P6 B3NMY9 A0A1W4VRW6 A0A1A9WIG7 W8CAP2 B4JWH2 A0A1A9Y4G9 Q6XI14 B4KS35 A0A0K8VVB1 A0A182JK70 B4I7T3 Q9W2E8 A0A0C9RCQ4 A0A034WSU9 A0A0J9RHS8 A0A182Y1K0 A0A182MYS8 A0A182WF03 A0A1A9ZZB1 A0A182JY89 A0A182UVF4 B3MIX7 A0A2R7VNV4 A0A084WID1 A0A182PJE9 A0A182KSD6 A0A182QLA6 A0A182RFF1 A0A182STG2 T1E7X6 A0A2M4ABB8 A0A2M4CRK4 A0A3F2Z221 A0A2C9GR74 A0A182TNS7 Q7PV97 A0A182LST2 A0A2M3Z7G4 B4MNH2 B4LPM9 A0A2M4C3Y5 A0A1B0FC36 A0A0K8TNQ9 A0A067RBD5 N6U8I6 A0A1I8MBK2 Q292B1 B4G9Y4 A0A3B0IZM2 A0A1I8PXW6 D6WQQ4 A0A0M4E564 T1GLA8 A0A1L8EFA2 A0A1L8EET6 A0A1J1HPJ3 A0A2J7R4H6 B0X345 A0A1Q3FL85 V5I822 A0A2P6L0V8 A0A182FBA4 A0A293M2J7 A0A2S2NW96 A0A1L8D9P9 A0A3R7PP15 A0A336MCG7 A0A336N127 A0A0P5UYT2 A0A2P8YX72 A0A1B0CM22 A0A0P5GHL8 R4WTB8 A0A1W0X323 A0A151WFS8 A0A2G8L0N8 A0A026W8V6 A0A195FXN7 A0A151J0Z6 A0A1B0GPS8 U5ERX7 A0A1B6LA68 A0A2R5LDB2 A0A1S4EL69 A0A0P5XLI6
A0A0N1IPA4 A0A2W1BAY6 A0A0N1PFF7 I4DJV3 A0A1E1WKQ2 A0A0L0BPF2 A0A023ECZ1 Q173P6 B3NMY9 A0A1W4VRW6 A0A1A9WIG7 W8CAP2 B4JWH2 A0A1A9Y4G9 Q6XI14 B4KS35 A0A0K8VVB1 A0A182JK70 B4I7T3 Q9W2E8 A0A0C9RCQ4 A0A034WSU9 A0A0J9RHS8 A0A182Y1K0 A0A182MYS8 A0A182WF03 A0A1A9ZZB1 A0A182JY89 A0A182UVF4 B3MIX7 A0A2R7VNV4 A0A084WID1 A0A182PJE9 A0A182KSD6 A0A182QLA6 A0A182RFF1 A0A182STG2 T1E7X6 A0A2M4ABB8 A0A2M4CRK4 A0A3F2Z221 A0A2C9GR74 A0A182TNS7 Q7PV97 A0A182LST2 A0A2M3Z7G4 B4MNH2 B4LPM9 A0A2M4C3Y5 A0A1B0FC36 A0A0K8TNQ9 A0A067RBD5 N6U8I6 A0A1I8MBK2 Q292B1 B4G9Y4 A0A3B0IZM2 A0A1I8PXW6 D6WQQ4 A0A0M4E564 T1GLA8 A0A1L8EFA2 A0A1L8EET6 A0A1J1HPJ3 A0A2J7R4H6 B0X345 A0A1Q3FL85 V5I822 A0A2P6L0V8 A0A182FBA4 A0A293M2J7 A0A2S2NW96 A0A1L8D9P9 A0A3R7PP15 A0A336MCG7 A0A336N127 A0A0P5UYT2 A0A2P8YX72 A0A1B0CM22 A0A0P5GHL8 R4WTB8 A0A1W0X323 A0A151WFS8 A0A2G8L0N8 A0A026W8V6 A0A195FXN7 A0A151J0Z6 A0A1B0GPS8 U5ERX7 A0A1B6LA68 A0A2R5LDB2 A0A1S4EL69 A0A0P5XLI6
EC Number
2.4.2.29
Pubmed
19121390
22118469
23622113
26227816
26354079
28756777
+ More
22651552 26108605 24945155 26483478 17510324 17994087 18057021 24495485 14525923 17550304 10731132 12537568 12537572 12537573 12537574 16110336 17569856 17569867 26109357 26109356 25348373 22936249 25244985 24438588 20966253 12364791 14747013 17210077 26369729 24845553 23537049 25315136 15632085 23185243 18362917 19820115 29403074 23691247 29023486 24508170 30249741
22651552 26108605 24945155 26483478 17510324 17994087 18057021 24495485 14525923 17550304 10731132 12537568 12537572 12537573 12537574 16110336 17569856 17569867 26109357 26109356 25348373 22936249 25244985 24438588 20966253 12364791 14747013 17210077 26369729 24845553 23537049 25315136 15632085 23185243 18362917 19820115 29403074 23691247 29023486 24508170 30249741
EMBL
BABH01040444
RSAL01000415
RVE41825.1
AGBW02008387
OWR53522.1
GAIX01006095
+ More
JAA86465.1 JTDY01004905 KOB67607.1 NWSH01004480 PCG65056.1 KQ460480 KPJ14341.1 KZ150386 PZC71064.1 KQ458842 KPJ04893.1 AK401571 BAM18193.1 GDQN01003482 JAT87572.1 JRES01001654 KNC21109.1 JXUM01050108 GAPW01006325 KQ561634 JAC07273.1 KXJ77935.1 CH477418 EAT41291.1 CH954179 EDV55483.1 KQS62305.1 GAMC01005571 JAC00985.1 CH916375 EDV98310.1 AY232018 CM000158 AAR10041.1 EDW91214.1 CH933808 EDW10471.1 KRG05273.1 GDHF01020460 GDHF01009510 JAI31854.1 JAI42804.1 CH480824 EDW56658.1 AE013599 BT031040 AAF46744.1 ABV53870.1 ABV53871.1 ABV82422.1 ACZ94512.1 ACZ94513.1 GBYB01010902 GBYB01010903 JAG80669.1 JAG80670.1 GAKP01000311 GAKP01000310 JAC58641.1 CM002911 KMY95603.1 KMY95604.1 CH902619 EDV37043.1 KPU76579.1 KK854009 PTY09232.1 ATLV01023929 KE525347 KFB49975.1 AXCN02001027 GAMD01003263 JAA98327.1 GGFK01004587 MBW37908.1 GGFL01003788 MBW67966.1 APCN01003304 AAAB01008986 EAA00203.3 AXCM01001727 GGFM01003694 MBW24445.1 CH963848 EDW73661.1 CH940648 EDW60267.1 KRF79330.1 GGFJ01010863 MBW60004.1 CCAG010005008 GDAI01001576 JAI16027.1 KK852570 KDR21146.1 APGK01035812 KB740928 KB631763 ENN77980.1 ERL85964.1 CM000071 EAL24951.1 KRT01722.1 KRT01723.1 KRT01724.1 CH479181 EDW31736.1 OUUW01000001 SPP73567.1 KQ971354 EFA07439.1 CP012524 ALC41354.1 CAQQ02026328 GFDG01001566 JAV17233.1 GFDG01001567 JAV17232.1 CVRI01000006 CRK88398.1 NEVH01007419 PNF35715.1 DS232308 EDS39547.1 GFDL01006773 JAV28272.1 GALX01005440 JAB63026.1 MWRG01002822 PRD32211.1 GFWV01010403 MAA35132.1 GGMR01008831 MBY21450.1 GFDF01010893 JAV03191.1 QCYY01001421 ROT78173.1 UFQS01000917 UFQT01000917 SSX07647.1 SSX27985.1 UFQT01003723 SSX35229.1 GDIP01106866 JAL96848.1 PYGN01000306 PSN48825.1 AJWK01018073 GDIQ01240918 JAK10807.1 AK417967 BAN21182.1 MTYJ01000020 OQV21885.1 KQ983203 KYQ46697.1 MRZV01000272 PIK53836.1 KK107324 QOIP01000006 EZA52527.1 RLU22031.1 KQ981193 KYN45198.1 KQ980575 KYN15469.1 AJVK01034701 GANO01003518 JAB56353.1 GEBQ01019573 JAT20404.1 GGLE01003340 MBY07466.1 GDIP01070321 JAM33394.1
JAA86465.1 JTDY01004905 KOB67607.1 NWSH01004480 PCG65056.1 KQ460480 KPJ14341.1 KZ150386 PZC71064.1 KQ458842 KPJ04893.1 AK401571 BAM18193.1 GDQN01003482 JAT87572.1 JRES01001654 KNC21109.1 JXUM01050108 GAPW01006325 KQ561634 JAC07273.1 KXJ77935.1 CH477418 EAT41291.1 CH954179 EDV55483.1 KQS62305.1 GAMC01005571 JAC00985.1 CH916375 EDV98310.1 AY232018 CM000158 AAR10041.1 EDW91214.1 CH933808 EDW10471.1 KRG05273.1 GDHF01020460 GDHF01009510 JAI31854.1 JAI42804.1 CH480824 EDW56658.1 AE013599 BT031040 AAF46744.1 ABV53870.1 ABV53871.1 ABV82422.1 ACZ94512.1 ACZ94513.1 GBYB01010902 GBYB01010903 JAG80669.1 JAG80670.1 GAKP01000311 GAKP01000310 JAC58641.1 CM002911 KMY95603.1 KMY95604.1 CH902619 EDV37043.1 KPU76579.1 KK854009 PTY09232.1 ATLV01023929 KE525347 KFB49975.1 AXCN02001027 GAMD01003263 JAA98327.1 GGFK01004587 MBW37908.1 GGFL01003788 MBW67966.1 APCN01003304 AAAB01008986 EAA00203.3 AXCM01001727 GGFM01003694 MBW24445.1 CH963848 EDW73661.1 CH940648 EDW60267.1 KRF79330.1 GGFJ01010863 MBW60004.1 CCAG010005008 GDAI01001576 JAI16027.1 KK852570 KDR21146.1 APGK01035812 KB740928 KB631763 ENN77980.1 ERL85964.1 CM000071 EAL24951.1 KRT01722.1 KRT01723.1 KRT01724.1 CH479181 EDW31736.1 OUUW01000001 SPP73567.1 KQ971354 EFA07439.1 CP012524 ALC41354.1 CAQQ02026328 GFDG01001566 JAV17233.1 GFDG01001567 JAV17232.1 CVRI01000006 CRK88398.1 NEVH01007419 PNF35715.1 DS232308 EDS39547.1 GFDL01006773 JAV28272.1 GALX01005440 JAB63026.1 MWRG01002822 PRD32211.1 GFWV01010403 MAA35132.1 GGMR01008831 MBY21450.1 GFDF01010893 JAV03191.1 QCYY01001421 ROT78173.1 UFQS01000917 UFQT01000917 SSX07647.1 SSX27985.1 UFQT01003723 SSX35229.1 GDIP01106866 JAL96848.1 PYGN01000306 PSN48825.1 AJWK01018073 GDIQ01240918 JAK10807.1 AK417967 BAN21182.1 MTYJ01000020 OQV21885.1 KQ983203 KYQ46697.1 MRZV01000272 PIK53836.1 KK107324 QOIP01000006 EZA52527.1 RLU22031.1 KQ981193 KYN45198.1 KQ980575 KYN15469.1 AJVK01034701 GANO01003518 JAB56353.1 GEBQ01019573 JAT20404.1 GGLE01003340 MBY07466.1 GDIP01070321 JAM33394.1
Proteomes
UP000005204
UP000283053
UP000007151
UP000037510
UP000218220
UP000053240
+ More
UP000053268 UP000037069 UP000069940 UP000249989 UP000008820 UP000008711 UP000192221 UP000091820 UP000001070 UP000092443 UP000002282 UP000009192 UP000075880 UP000001292 UP000000803 UP000076408 UP000075884 UP000075920 UP000092445 UP000075881 UP000075903 UP000007801 UP000030765 UP000075885 UP000075882 UP000075886 UP000075900 UP000075901 UP000076407 UP000075840 UP000075902 UP000007062 UP000075883 UP000007798 UP000008792 UP000092444 UP000027135 UP000019118 UP000030742 UP000095301 UP000001819 UP000008744 UP000268350 UP000095300 UP000007266 UP000092553 UP000015102 UP000183832 UP000235965 UP000002320 UP000069272 UP000283509 UP000245037 UP000092461 UP000075809 UP000230750 UP000053097 UP000279307 UP000078541 UP000078492 UP000092462 UP000079169
UP000053268 UP000037069 UP000069940 UP000249989 UP000008820 UP000008711 UP000192221 UP000091820 UP000001070 UP000092443 UP000002282 UP000009192 UP000075880 UP000001292 UP000000803 UP000076408 UP000075884 UP000075920 UP000092445 UP000075881 UP000075903 UP000007801 UP000030765 UP000075885 UP000075882 UP000075886 UP000075900 UP000075901 UP000076407 UP000075840 UP000075902 UP000007062 UP000075883 UP000007798 UP000008792 UP000092444 UP000027135 UP000019118 UP000030742 UP000095301 UP000001819 UP000008744 UP000268350 UP000095300 UP000007266 UP000092553 UP000015102 UP000183832 UP000235965 UP000002320 UP000069272 UP000283509 UP000245037 UP000092461 UP000075809 UP000230750 UP000053097 UP000279307 UP000078541 UP000078492 UP000092462 UP000079169
Interpro
SUPFAM
SSF51713
SSF51713
Gene 3D
ProteinModelPortal
H9JI34
A0A437AUS2
A0A212FIG3
S4PGR4
A0A0L7KWG6
A0A2A4IZY4
+ More
A0A0N1IPA4 A0A2W1BAY6 A0A0N1PFF7 I4DJV3 A0A1E1WKQ2 A0A0L0BPF2 A0A023ECZ1 Q173P6 B3NMY9 A0A1W4VRW6 A0A1A9WIG7 W8CAP2 B4JWH2 A0A1A9Y4G9 Q6XI14 B4KS35 A0A0K8VVB1 A0A182JK70 B4I7T3 Q9W2E8 A0A0C9RCQ4 A0A034WSU9 A0A0J9RHS8 A0A182Y1K0 A0A182MYS8 A0A182WF03 A0A1A9ZZB1 A0A182JY89 A0A182UVF4 B3MIX7 A0A2R7VNV4 A0A084WID1 A0A182PJE9 A0A182KSD6 A0A182QLA6 A0A182RFF1 A0A182STG2 T1E7X6 A0A2M4ABB8 A0A2M4CRK4 A0A3F2Z221 A0A2C9GR74 A0A182TNS7 Q7PV97 A0A182LST2 A0A2M3Z7G4 B4MNH2 B4LPM9 A0A2M4C3Y5 A0A1B0FC36 A0A0K8TNQ9 A0A067RBD5 N6U8I6 A0A1I8MBK2 Q292B1 B4G9Y4 A0A3B0IZM2 A0A1I8PXW6 D6WQQ4 A0A0M4E564 T1GLA8 A0A1L8EFA2 A0A1L8EET6 A0A1J1HPJ3 A0A2J7R4H6 B0X345 A0A1Q3FL85 V5I822 A0A2P6L0V8 A0A182FBA4 A0A293M2J7 A0A2S2NW96 A0A1L8D9P9 A0A3R7PP15 A0A336MCG7 A0A336N127 A0A0P5UYT2 A0A2P8YX72 A0A1B0CM22 A0A0P5GHL8 R4WTB8 A0A1W0X323 A0A151WFS8 A0A2G8L0N8 A0A026W8V6 A0A195FXN7 A0A151J0Z6 A0A1B0GPS8 U5ERX7 A0A1B6LA68 A0A2R5LDB2 A0A1S4EL69 A0A0P5XLI6
A0A0N1IPA4 A0A2W1BAY6 A0A0N1PFF7 I4DJV3 A0A1E1WKQ2 A0A0L0BPF2 A0A023ECZ1 Q173P6 B3NMY9 A0A1W4VRW6 A0A1A9WIG7 W8CAP2 B4JWH2 A0A1A9Y4G9 Q6XI14 B4KS35 A0A0K8VVB1 A0A182JK70 B4I7T3 Q9W2E8 A0A0C9RCQ4 A0A034WSU9 A0A0J9RHS8 A0A182Y1K0 A0A182MYS8 A0A182WF03 A0A1A9ZZB1 A0A182JY89 A0A182UVF4 B3MIX7 A0A2R7VNV4 A0A084WID1 A0A182PJE9 A0A182KSD6 A0A182QLA6 A0A182RFF1 A0A182STG2 T1E7X6 A0A2M4ABB8 A0A2M4CRK4 A0A3F2Z221 A0A2C9GR74 A0A182TNS7 Q7PV97 A0A182LST2 A0A2M3Z7G4 B4MNH2 B4LPM9 A0A2M4C3Y5 A0A1B0FC36 A0A0K8TNQ9 A0A067RBD5 N6U8I6 A0A1I8MBK2 Q292B1 B4G9Y4 A0A3B0IZM2 A0A1I8PXW6 D6WQQ4 A0A0M4E564 T1GLA8 A0A1L8EFA2 A0A1L8EET6 A0A1J1HPJ3 A0A2J7R4H6 B0X345 A0A1Q3FL85 V5I822 A0A2P6L0V8 A0A182FBA4 A0A293M2J7 A0A2S2NW96 A0A1L8D9P9 A0A3R7PP15 A0A336MCG7 A0A336N127 A0A0P5UYT2 A0A2P8YX72 A0A1B0CM22 A0A0P5GHL8 R4WTB8 A0A1W0X323 A0A151WFS8 A0A2G8L0N8 A0A026W8V6 A0A195FXN7 A0A151J0Z6 A0A1B0GPS8 U5ERX7 A0A1B6LA68 A0A2R5LDB2 A0A1S4EL69 A0A0P5XLI6
PDB
5XTI
E-value=7.10158e-09,
Score=137
Ontologies
PATHWAY
GO
Topology
Subcellular location
Cytoplasm
Length:
101
Number of predicted TMHs:
1
Exp number of AAs in TMHs:
18.39408
Exp number, first 60 AAs:
0.00088
Total prob of N-in:
0.92590
inside
1 - 63
TMhelix
64 - 82
outside
83 - 101
Population Genetic Test Statistics
Pi
32.991531
Theta
23.965834
Tajima's D
1.342225
CLR
0.352429
CSRT
0.75661216939153
Interpretation
Uncertain
