Gene

KWMTBOMO12291

Pre Gene Modal

BGIBMGA009157

Annotation

PREDICTED:_ubiquitin-conjugating_enzyme_E2_T-like_[Papilio_polytes]

Location

Bomo_Chr20(+):11597135-11598228      View in EpiBrowser

Full name

Ubiquitin-conjugating enzyme E2 T      

Alternative Name

Cell proliferation-inducing gene 50 protein
E2 ubiquitin-conjugating enzyme T
Ubiquitin carrier protein T
Ubiquitin-protein ligase T

Location in the cell

Mitochondrial   Reliability : 1.4

CDS

ATGCTATCAAGTGCTAGAGCTGCTCGCATCGCGCGTGAGGTGAAGAATTTTGAAATGAAGCCACCTTGGGGGATTAGTTGTTTTCCGTTAAAAGACGATCAGTATGACAACCTGTCCGTTATTCTTCTTGGACCAAAGGGATCTCCTTACGAGAAAGGAACGTTCAAATTAACTGTGTCTATCCCGGAAAGATATCCGTTCGAGCCTCCTTTAGTTAAATTTATAACACCAGTTTATCATCCTAATATTGACAATGGAGGAAGAATATGTATGGACCTGTTAAAAATGCCACCAAAAGGTACTTGGTTACCGACGATCACACTGGAAACGTTACTTGTTTCATTACAATCACTCCTCGCGAACCCAAACCCTGATGACCCACTGATGATGGATATCGCTTATGAGTATAAATACAACATGGATACTTTTATTCAGAATGCTAAACAACAAACTGAGAAATATGCTGTGTGA

Protein

MLSSARAARIAREVKNFEMKPPWGISCFPLKDDQYDNLSVILLGPKGSPYEKGTFKLTVSIPERYPFEPPLVKFITPVYHPNIDNGGRICMDLLKMPPKGTWLPTITLETLLVSLQSLLANPNPDDPLMMDIAYEYKYNMDTFIQNAKQQTEKYAV

Description

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in mitomycin-C (MMC)-induced DNA repair. Acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway (PubMed:16916645, PubMed:17938197, PubMed:19111657, PubMed:19589784, PubMed:28437106). Also mediates monoubiquitination of FANCL and FANCI (PubMed:16916645, PubMed:17938197, PubMed:19111657, PubMed:19589784). May contribute to ubiquitination and degradation of BRCA1 (PubMed:19887602). In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11'-, 'Lys-27'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination (PubMed:20061386).

Catalytic Activity

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.

Subunit

Directly interacts with FANCL (PubMed:16916645, PubMed:17938197, PubMed:19111657, PubMed:21775430, PubMed:24389026). Interacts with BRCA1 (PubMed:19887602).

Similarity

Belongs to the ubiquitin-conjugating enzyme family.

Keywords

3D-structure   ATP-binding   Complete proteome   Disease mutation   DNA damage   DNA repair   Fanconi anemia   Isopeptide bond   Nucleotide-binding   Nucleus   Phosphoprotein   Reference proteome   Transferase   Ubl conjugation   Ubl conjugation pathway  

Feature

chain  Ubiquitin-conjugating enzyme E2 T
sequence variant  In FANCT; abolishes FANCD2 monoubiquitination; abolishes interaction with FANCL; dbSNP:rs774357609.

Uniprot

H9JI09   A0A1E1W591   A0A2H1WVW4   A0A2W1BXK9   A0A0N0PB08   S4P9M7   + More
A0A212FCY5   A0A1S2ZNM2   A0A2K5RKL7   A0A0L7LHY2   A0A2K5N119   H0VGI7   M3YIB2   M3VUS9   A0A2K5DBL8   G9KWA0   A0A3Q7MW60   A0A2Y9GG91   A0A2U3Z1R0   A0A2U3VED0   U3EFD1   F7IHR0   H2Q0X0   E2RGY5   A0A3Q7T9J5   A0A2R9B0J5   H2N457   A0A024R9A9   Q9NPD8   A0A0D9RNT6   A0A096NB93   A0A2K6QDU6   A0A2K5I644   A0A2K5XBP1   A0A2K6LV72   G7NWE1   A0A2K6EC41   H9EWW6   G1S8Z3   A0A2K6S7G1   A0A2K6LV87   U6CUU1   G1LZJ5   G7MEY1   K7G1F3   A0A091RFE2   D2GZM2   A0A2Y9K4D8   A0A2K6LV95   H0XUT4   F1S5B2   G3QQW9   A0A091D094   G1SYX6   A0A1U7UQG7   G3WJY5   I3MFF5   A0A3Q7UJB5   A0A1S3FAC4   A0A2K6GBM8   K4FYB0   I0YJL6   A0A2Y9FNY7   A0A0A0A8M1   A0A093TNA1   A0A2Y9DIJ4   A0A094L9D6   A0A1S3IFL0   A7RL90   A0A2Y9M6H5   A0A1V4KX15   A0A226NHS8   V9L5S3   A0A2I0LZT0   A0A2U3VA14   G3TEE8   G5B384   A0A091EU64   A0A151N9Q8   A0A1U7SFP4   A0A093H712   A0A226NS10   A0A384BL62   A0A2U4C6R1   G1N182   L5KWK3   R0KSE2   A0A3P9LT27   U3J9J1   A0A341BRS9   A0A3P9JRY0   F6TGJ9   A0A3B4GEL3   A0A3P8P508   A0A3P9DQ64   A0A2P4T4Y4   A0A087R707   L8I9K1   I3K351   A0A3Q0S6K7  

EC Number

2.3.2.23

Pubmed

19121390    28756777    26354079    23622113    22118469    26227816    + More
21993624    17975172    23236062    25243066    16136131    16341006    22722832    11181995    10931946    11042152    14702039    16710414    15489334    16916645    17938197    19111657    18669648    19887602    19589784    20061386    21269460    21775430    21722982    23186163    26046368    28112733    22496338    24389026    28437106    25362486    22002653    17431167    25319552    20010809    17381049    30723633    21709235    23056606    22630137    26383154    17615350    24402279    23371554    21993625    22293439    24621616    24813606    20838655    23258410    17554307    23749191    18464734    25186727    22751099   

EMBL

BABH01035375    GDQN01011427    GDQN01008909    JAT79627.1    JAT82145.1    ODYU01011460    + More
SOQ57203.1    KZ149926    PZC77550.1    KQ461072    KPJ09825.1    GAIX01006767    JAA85793.1    AGBW02009135    OWR51577.1    JTDY01001114    KOB74831.1    AAKN02017947    AEYP01041155    AANG04001043    JP020581    AES09179.1    GAMR01009641    JAB24291.1    AACZ04071412    GABC01009151    GABF01006910    GABD01006468    GABE01008854    NBAG03000011    JAA02187.1    JAA15235.1    JAA26632.1    JAA35885.1    PNJ00663.1    AAEX03004980    AAEX03004981    AJFE02080327    AJFE02080328    AJFE02080329    ABGA01298331    NDHI03003439    PNJ49951.1    CH471067    EAW91415.1    AB032931    AF160215    AF161499    AK000504    AY542309    AL356953    BC004152    BC019284    AQIB01112748    AQIB01112749    AHZZ02017365    AQIA01001381    CM001276    EHH50653.1    JSUE03003024    JU323119    JU475284    AFE66875.1    AFH32088.1    ADFV01023368    HAAF01002420    CCP74246.1    ACTA01113567    CM001253    EHH15678.1    AGCU01037467    AGCU01037468    AGCU01037469    KK815917    KFQ38221.1    GL192393    EFB26772.1    AAQR03089361    AAQR03089362    AEMK02000073    DQIR01035870    HCZ91345.1    CABD030008325    CABD030008327    KN123586    KFO24402.1    AAGW02025277    AEFK01157657    AEFK01157658    AGTP01062255    JX052919    AFK11147.1    AGSI01000023    EIE18585.1    KL871087    KGL90292.1    KL446871    KFW95659.1    KL353698    KFZ60644.1    DS469517    EDO47887.1    LSYS01001493    OPJ88956.1    MCFN01000042    OXB67245.1    JW874368    AFP06885.1    AKCR02000055    PKK22937.1    JH168168    EHB03745.1    KK718915    KFO59859.1    AKHW03003787    KYO33225.1    KL205940    KFV77441.1    AWGT02001417    OXB70575.1    KB030502    ELK15834.1    KB744057    EOA96188.1    ADON01119723    PPHD01008799    POI31397.1    KL226151    KFM09261.1    JH881618    ELR53140.1    AERX01019465   

Proteomes

UP000005204    UP000053240    UP000007151    UP000079721    UP000233040    UP000037510    + More
UP000233060    UP000005447    UP000000715    UP000011712    UP000233020    UP000286641    UP000248481    UP000245341    UP000245340    UP000008225    UP000002277    UP000002254    UP000286640    UP000240080    UP000001595    UP000005640    UP000029965    UP000028761    UP000233200    UP000233080    UP000233140    UP000233180    UP000009130    UP000233100    UP000233120    UP000006718    UP000001073    UP000233220    UP000008912    UP000007267    UP000248482    UP000005225    UP000008227    UP000001519    UP000028990    UP000001811    UP000189704    UP000007648    UP000005215    UP000286642    UP000081671    UP000233160    UP000007264    UP000248484    UP000053858    UP000248480    UP000085678    UP000001593    UP000248483    UP000190648    UP000198323    UP000053872    UP000245320    UP000007646    UP000006813    UP000052976    UP000050525    UP000189705    UP000053584    UP000198419    UP000261680    UP000291021    UP000001645    UP000010552    UP000265180    UP000016666    UP000252040    UP000265200    UP000002279    UP000261460    UP000265100    UP000265160    UP000053286    UP000005207    UP000261340   

PRIDE

H9JI09    A0A024R9A9    Q9NPD8    F1S5B2   

Pfam

PF00179   UQ_con

Interpro

IPR016135   UBQ-conjugating_enzyme/RWD        + More
IPR000608   UBQ-conjugat_E2       
IPR023313   UBQ-conjugating_AS       

SUPFAM

SSF54495   SSF54495       

Gene 3D

3.10.110.10    

CDD

cd00195  UBCc

ProteinModelPortal

H9JI09   A0A1E1W591   A0A2H1WVW4   A0A2W1BXK9   A0A0N0PB08   S4P9M7   + More
A0A212FCY5   A0A1S2ZNM2   A0A2K5RKL7   A0A0L7LHY2   A0A2K5N119   H0VGI7   M3YIB2   M3VUS9   A0A2K5DBL8   G9KWA0   A0A3Q7MW60   A0A2Y9GG91   A0A2U3Z1R0   A0A2U3VED0   U3EFD1   F7IHR0   H2Q0X0   E2RGY5   A0A3Q7T9J5   A0A2R9B0J5   H2N457   A0A024R9A9   Q9NPD8   A0A0D9RNT6   A0A096NB93   A0A2K6QDU6   A0A2K5I644   A0A2K5XBP1   A0A2K6LV72   G7NWE1   A0A2K6EC41   H9EWW6   G1S8Z3   A0A2K6S7G1   A0A2K6LV87   U6CUU1   G1LZJ5   G7MEY1   K7G1F3   A0A091RFE2   D2GZM2   A0A2Y9K4D8   A0A2K6LV95   H0XUT4   F1S5B2   G3QQW9   A0A091D094   G1SYX6   A0A1U7UQG7   G3WJY5   I3MFF5   A0A3Q7UJB5   A0A1S3FAC4   A0A2K6GBM8   K4FYB0   I0YJL6   A0A2Y9FNY7   A0A0A0A8M1   A0A093TNA1   A0A2Y9DIJ4   A0A094L9D6   A0A1S3IFL0   A7RL90   A0A2Y9M6H5   A0A1V4KX15   A0A226NHS8   V9L5S3   A0A2I0LZT0   A0A2U3VA14   G3TEE8   G5B384   A0A091EU64   A0A151N9Q8   A0A1U7SFP4   A0A093H712   A0A226NS10   A0A384BL62   A0A2U4C6R1   G1N182   L5KWK3   R0KSE2   A0A3P9LT27   U3J9J1   A0A341BRS9   A0A3P9JRY0   F6TGJ9   A0A3B4GEL3   A0A3P8P508   A0A3P9DQ64   A0A2P4T4Y4   A0A087R707   L8I9K1   I3K351   A0A3Q0S6K7  

PDB

4CCG     E-value=1.04163e-41,     Score=422

KEGG

101739500    K13960 ubiquitin-conjugating enzyme E2 T [EC:2.3.2.23] | (RefSeq) ubiquitin-conjugating enzyme E2 T-

PATHWAY

03460    Fanconi anemia pathway - Bombyx mori (domestic silkworm)

GO

GO:0016740   GO:0005524   GO:0085020   GO:0070534   GO:0005730   GO:0031625   GO:0061631   GO:0035519   GO:0070936   GO:0006513   GO:0003682   GO:0006281   GO:0051865   GO:0070979   GO:0044314   GO:0005634   GO:0006974   GO:0004842   GO:0005654   GO:0036297   GO:0016567  

Subcellular location

Nucleus  

Length:

156

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.26003

Exp number, first 60 AAs:

0

Total prob of N-in:

0.23007

outside

1  -  156

 

 

Pi

31.99505

Theta

30.698008

Tajima's D

-0.029126

CLR

0

CSRT

0.363681815909205

Interpretation

Uncertain