Gene

KWMTBOMO12241  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA014194

Annotation

PREDICTED:_vacuolar_protein_sorting-associated_protein_35_isoform_X1_[Bombyx_mori]

Location

Bomo_Chr20(+):10299210-10306897      View in EpiBrowser

Full name

Vacuolar protein sorting-associated protein 35      

Alternative Name

Maternal-embryonic 3
Vesicle protein sorting 35

Location in the cell

Cytoplasmic   Reliability : 1.607 Nuclear   Reliability : 1.021

CDS

ATGAAGATCATCAAGCTGCCCTGCGACCACTACAAGAACATACTGGTGCTGATTAAGTTGTCCAATTACGCGCCGCTGATACGACATCTCAGCTACCAGGGTCGGGTGATGATAGCCGTCCACCTCATCAACGACGTGCTGGAGACCAACACCGCAATATCCACTCCGCAAGATGTCGAAGCTGTCCTCGGTCTCTTGGATGTGCTGGTGAAGGATCAGCCGGATCAGCCGCCTTCTGAAAGCGACGTTGAAGACTTCGTGGAAGAACAGGGCTTGTTGGCCAGACTTATTCATCATTTCAAATCGGACTCCGCCGACCAGCAGTATCTGATAACCAGCACAGCGAGGAAGATCCTTCAGGGCGGCGGAGCGAGGAGGATCCAGCACACGTTCCCGCCGTTGGTGTTCCACGCGTACCGTCTGGCGTTCGTTTATAAGGACCTCAAAGACCAGGACGAGATGTGGGAGAAGAAATGCCAGAAGATATTCCAGTTCTGTCACCAGACCATCACCATGCTCGTGAAGGCGGAACTGGCTGAACTCCCTCTAAGGTTGTACCTCCAGGGAGCTTTGGCGATAAGCGAAATAGGATTTGCCAATCATGAAACGATAGCTTACGAGTTCCTGTCGCAAGCTTTCTCGCTGTATGAAGACGAGATATCCGACAGCAAAGCGCAACTGGCCGCAATAACATTGATAATAGCCACCTTCGAGCAGATCAATTGTTTTGGCGCTGAGAACGCGGAGCCGATGCGGACCCAGTGCGCGCTGGCGGCCAGCAAGCTGCTGAAGAAGCCGGACCAGAGCAGGGCCGTGGCGCTCTGCTCGCATCTCTTCTGGAAGGGGACCAGGGACGGCCGCCCGGTGTGGGCGCTGAACGACGCGTCCCGCGCGCTGGACTGCCTGAAGAAGGCGGCCCGCGTGGCCCAGCAGTGCATGGACGGCGGCGTGCAGGCGCAGCTGCTGGCCGAGCTGCTGGGCCGATACGCGCTGCTGCGGGAGCGCGGCAACCAGAGTCTCACGCCCAGCCTCATCGACGCGATAATACAGAAAATACGCGAGGAGCTGGCCAACTTAGATCAGTCCGAAGAAGTGGAGCAGATCACGAAACACTTCCACAACACTTTGCAGCATCTGAAGAACAGGATCGAGTCTCCGGACCCCGAGAGTCTGGGCTACGAGGGTCTCACGCTGTCCTAA

Protein

MKIIKLPCDHYKNILVLIKLSNYAPLIRHLSYQGRVMIAVHLINDVLETNTAISTPQDVEAVLGLLDVLVKDQPDQPPSESDVEDFVEEQGLLARLIHHFKSDSADQQYLITSTARKILQGGGARRIQHTFPPLVFHAYRLAFVYKDLKDQDEMWEKKCQKIFQFCHQTITMLVKAELAELPLRLYLQGALAISEIGFANHETIAYEFLSQAFSLYEDEISDSKAQLAAITLIIATFEQINCFGAENAEPMRTQCALAASKLLKKPDQSRAVALCSHLFWKGTRDGRPVWALNDASRALDCLKKAARVAQQCMDGGVQAQLLAELLGRYALLRERGNQSLTPSLIDAIIQKIREELANLDQSEEVEQITKHFHNTLQHLKNRIESPDPESLGYEGLTLS

Description

Plays a role in vesicular protein sorting.
Acts as component of the retromer cargo-selective complex (CSC). The CSC is believed to be the core functional component of retromer or respective retromer complex variants acting to prevent missorting of selected transmembrane cargo proteins into the lysosomal degradation pathway. The recruitment of the CSC to the endosomal membrane involves RAB7A and SNX3. The CSC seems to associate with the cytoplasmic domain of cargo proteins predominantly via VPS35; however, these interactions seem to be of low affinity and retromer SNX proteins may also contribute to cargo selectivity thus questioning the classical function of the CSC. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX3-retromer mediates the retrograde transport of WLS distinct from the SNX-BAR retromer pathway. The SNX27-retromer is believed to be involved in endosome-to-plasma membrane trafficking and recycling of a broad spectrum of cargo proteins. The CSC seems to act as recruitment hub for other proteins, such as the WASH complex and TBC1D5 (Probable). Required for retrograde transport of lysosomal enzyme receptor IGF2R and SLC11A2. Required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). Required for endosomal localization of WASHC2 and mediates the association of the CSC with the WASH complex (By similarity).

Subunit

Component of the heterotrimeric retromer cargo-selective complex (CSC), also described as vacuolar protein sorting subcomplex (VPS) formed by VPS26 (VPS26A or VPS26B), VPS29 and VPS35. The CSC has a highly elongated structure with VPS26 and VPS29 binding independently at opposite distal ends of VPS35 as central platform (PubMed:21040701, PubMed:20875039, PubMed:21920005). The CSC is believed to associate with variable sorting nexins to form functionally distinct retromer complex variants. The originally described retromer complex (also called SNX-BAR retromer) is a pentamer containing the CSC and a heterodimeric membrane-deforming subcomplex formed between SNX1 or SNX2 and SNX5 or SNX6 (also called SNX-BAR subcomplex); the affinity between the respective CSC and SNX-BAR subcomplexes is low. The CSC associates with SNX3 to form a SNX3-retromer complex. The CSC associates with SNX27, the WASH complex and the SNX-BAR subcomplex to form the SNX27-retromer complex (Probable). Interacts with VPS26A, VPS29, VPS26B and LRRK2 (PubMed:16190980, PubMed:21040701, PubMed:20875039, PubMed:21920005, PubMed:23395371). Interacts with SNX1, SNX2, IGF2R, SNX3, GOLPH3, SLC11A2, WASHC2, FKBP15, WASHC1, EHD1. Interacts with MAGEL2; leading to recruitment of the TRIM27:MAGEL2 E3 ubiquitin ligase complex retromer-containing endosomes (By similarity).

Similarity

Belongs to the VPS35 family.

Keywords

Complete proteome   Cytoplasm   Direct protein sequencing   Endosome   Membrane   Phosphoprotein   Protein transport   Reference proteome   Transport  

Feature

chain  Vacuolar protein sorting-associated protein 35

Uniprot

A0A194QFH4   A0A1E1WPM0   A0A2A4J3P7   H9JXC9   A0A0N0PAV2   A0A2Z5U7Y2   + More
A0A2W1BHY5   A0A1B6BWC4   A0A1Y1L5K3   A0A1Y1L104   A0A154PEM6   A0A026X171   A0A3L8DR35   A0A1B6JB37   A0A0L7RH01   A0A0J7KZE6   A0A0N0BFA1   A0A1B6LFS4   F4WW33   A0A310SSW8   A0A195B1Z9   A0A158NLJ4   A0A195CRL7   A0A195E754   A0A2J7RBJ3   A0A195EYX3   A0A151XKC0   A0A0C9R1A1   A0A0C9RRG2   A0A0C9Q717   E2BGH6   A0A067REE6   A0A0T6B773   A0A087ZYP1   E2B0Q3   A0A2A3EEH7   K7ITP4   A0A093ESR9   C0PU95   A0A091L8E6   A0A2K6D3M2   A0A2K5WX53   A0A2I3T974   A0A1W4WZP8   I7GPC4   A0A2U3Z435   B5DFC1   K9KEJ7   V9K8I6   A4QP42   Q1ED30   A0A3Q0DXW8   A0A3P9AEM0   A0A2I0M2I5   A0A3L7HZ98   Q6ZM34   A0A061IC10   A0A3P8YVD1   A0A3B4G4Z7   A0A3P8PEY6   Q3TKU6   A0A3B3B7F9   A0A3S2N731   W5UH12   A0A2D0Q7L1   A0A091J4C6   A0A091FL01   Q3TRJ1   Q9EQH3   A0A1U7S401   A0A2I3MLH5   A0A093HKA9   A0A218UWD5   A0A1V4KPU8   R0JL21   A0A3L8S0K6   A0A093PHN3   A0A091UDZ5   A0A091JUI9   A0A099ZZN4   A0A093NWN7   A0A091RD27   A0A087QX31   H0ZF25   A0A060VUG2   W5LGY1   A0A099YWH3   A0A091NKN6   A0A091F1P6   A0A093CT46   A0A3B4G2N2   U3JBP3   A0A093QUD4   A0A1U7R5B8   A0A091TA01   A0A091V0H4   A0A2Y9M0G7   A0A341BYZ5   A0A2U4B467   A0A3Q3C0N9  

Pubmed

26354079    19121390    28756777    28004739    24508170    30249741    + More
21719571    21347285    20798317    24845553    20075255    16136131    17194215    15489334    24402279    25069045    23371554    29704459    23594743    23929341    10349636    11042159    11076861    11217851    12466851    16141073    29451363    23127152    12040188    17947660    19131326    21183079    8678978    11112353    16190980    21040701    20875039    21920005    23395371    30282656    20360741    24755649    25329095   

EMBL

KQ459053    KPJ04237.1    GDQN01002197    JAT88857.1    NWSH01003436    PCG66308.1    + More
BABH01035847    BABH01035848    BABH01035849    KQ461157    KPJ08096.1    AP017506    BBB06811.1    KZ150152    PZC72827.1    GEDC01031715    JAS05583.1    GEZM01067994    JAV67275.1    GEZM01067995    JAV67274.1    KQ434878    KZC09884.1    KK107063    EZA61104.1    QOIP01000005    RLU22742.1    GECU01011358    GECU01008054    JAS96348.1    JAS99652.1    KQ414596    KOC70113.1    LBMM01001796    KMQ95736.1    KQ435804    KOX73031.1    GEBQ01017448    GEBQ01009775    JAT22529.1    JAT30202.1    GL888404    EGI61622.1    KQ759820    OAD62720.1    KQ976662    KYM78315.1    ADTU01019551    KQ977349    KYN03351.1    KQ979579    KYN20669.1    NEVH01005896    PNF38206.1    KQ981914    KYN33107.1    KQ982038    KYQ60787.1    GBYB01009924    JAG79691.1    GBYB01009923    GBYB01009926    JAG79690.1    JAG79693.1    GBYB01009928    JAG79695.1    GL448189    EFN85169.1    KK852686    KDR18449.1    LJIG01009391    KRT83174.1    GL444666    EFN60725.1    KZ288269    PBC29874.1    KK615337    KFV48357.1    BT072172    ACN58635.1    KL302788    KFP51548.1    AQIA01042781    AQIA01042782    AACZ04030068    AB174124    BAE91186.1    BC169004    AAI69004.1    JL641145    AEQ00924.1    JW861487    AFO94004.1    BC139644    AAI39645.1    BC117573    AAI17574.1    AKCR02000046    PKK23890.1    RAZU01000127    RLQ71266.1    AL928906    KE672984    ERE78825.1    AK166821    BAE39047.1    CM012439    RVE74984.1    JT410194    AHH38962.1    KL218569    KFP06661.1    KL447078    KFO69631.1    AK150682    AK162726    AK167421    AK168880    AK170473    CH466525    BAE29762.1    BAE37038.1    BAE39509.1    BAE40698.1    BAE41818.1    EDL11004.1    U47024    AF226323    BC005469    BC006637    AHZZ02018658    AHZZ02018659    AHZZ02018660    AHZZ02018661    AHZZ02018662    KL206526    KFV83103.1    MUZQ01000107    OWK58124.1    LSYS01002285    OPJ86464.1    KB743571    EOA97990.1    QUSF01000110    RLV91534.1    KL669378    KFW75941.1    KK430718    KFQ88944.1    KK501485    KFP15261.1    KL870252    KGL87266.1    KL224832    KFW64537.1    KK813735    KFQ37386.1    KL225962    KFM05785.1    ABQF01034926    FR904258    CDQ55990.1    KL885851    KGL73293.1    KL384598    KFP89464.1    KK719362    KFO63161.1    KL465352    KFV16064.1    AGTO01010909    KL433035    KFW92131.1    KK442340    KFQ70600.1    KL410289    KFQ95770.1   

Proteomes

UP000053268    UP000218220    UP000005204    UP000053240    UP000076502    UP000053097    + More
UP000279307    UP000053825    UP000036403    UP000053105    UP000007755    UP000078540    UP000005205    UP000078542    UP000078492    UP000235965    UP000078541    UP000075809    UP000008237    UP000027135    UP000005203    UP000000311    UP000242457    UP000002358    UP000233120    UP000233100    UP000002277    UP000192223    UP000245341    UP000189704    UP000265140    UP000053872    UP000273346    UP000000437    UP000030759    UP000261460    UP000265100    UP000261560    UP000221080    UP000054308    UP000053760    UP000000589    UP000189705    UP000028761    UP000053584    UP000197619    UP000190648    UP000276834    UP000053258    UP000053119    UP000053858    UP000054081    UP000053286    UP000007754    UP000193380    UP000018467    UP000053641    UP000052976    UP000016665    UP000189706    UP000053283    UP000248483    UP000252040    UP000245320    UP000264840   

PRIDE

H9JXC9    B5DFC1    Q3TKU6    Q9EQH3   

Pfam

PF03635   Vps35        + More
PF09747   DUF2052

Interpro

IPR005378   Vps35        + More
IPR040233   DUF2052       
IPR016024   ARM-type_fold       

SUPFAM

SSF48371   SSF48371       

ProteinModelPortal

A0A194QFH4   A0A1E1WPM0   A0A2A4J3P7   H9JXC9   A0A0N0PAV2   A0A2Z5U7Y2   + More
A0A2W1BHY5   A0A1B6BWC4   A0A1Y1L5K3   A0A1Y1L104   A0A154PEM6   A0A026X171   A0A3L8DR35   A0A1B6JB37   A0A0L7RH01   A0A0J7KZE6   A0A0N0BFA1   A0A1B6LFS4   F4WW33   A0A310SSW8   A0A195B1Z9   A0A158NLJ4   A0A195CRL7   A0A195E754   A0A2J7RBJ3   A0A195EYX3   A0A151XKC0   A0A0C9R1A1   A0A0C9RRG2   A0A0C9Q717   E2BGH6   A0A067REE6   A0A0T6B773   A0A087ZYP1   E2B0Q3   A0A2A3EEH7   K7ITP4   A0A093ESR9   C0PU95   A0A091L8E6   A0A2K6D3M2   A0A2K5WX53   A0A2I3T974   A0A1W4WZP8   I7GPC4   A0A2U3Z435   B5DFC1   K9KEJ7   V9K8I6   A4QP42   Q1ED30   A0A3Q0DXW8   A0A3P9AEM0   A0A2I0M2I5   A0A3L7HZ98   Q6ZM34   A0A061IC10   A0A3P8YVD1   A0A3B4G4Z7   A0A3P8PEY6   Q3TKU6   A0A3B3B7F9   A0A3S2N731   W5UH12   A0A2D0Q7L1   A0A091J4C6   A0A091FL01   Q3TRJ1   Q9EQH3   A0A1U7S401   A0A2I3MLH5   A0A093HKA9   A0A218UWD5   A0A1V4KPU8   R0JL21   A0A3L8S0K6   A0A093PHN3   A0A091UDZ5   A0A091JUI9   A0A099ZZN4   A0A093NWN7   A0A091RD27   A0A087QX31   H0ZF25   A0A060VUG2   W5LGY1   A0A099YWH3   A0A091NKN6   A0A091F1P6   A0A093CT46   A0A3B4G2N2   U3JBP3   A0A093QUD4   A0A1U7R5B8   A0A091TA01   A0A091V0H4   A0A2Y9M0G7   A0A341BYZ5   A0A2U4B467   A0A3Q3C0N9  

PDB

5OSI     E-value=2.9695e-99,     Score=924

KEGG

101737683    K18468 vacuolar protein sorting-associated protein 35 | (RefSeq) vacuolar protein sorting-associated

PATHWAY

04144    Endocytosis - Bombyx mori (domestic silkworm)

GO

GO:0005829   GO:0042147   GO:0030906   GO:0015031   GO:0016021   GO:0006886   GO:0005770   GO:0030904   GO:0010628   GO:0005769   GO:0097422   GO:0010008   GO:0005764   GO:2000331   GO:0031748   GO:1901215   GO:0099639   GO:0050728   GO:1902823   GO:0005739   GO:0099074   GO:0045056   GO:1903364   GO:0036010   GO:0043653   GO:0031647   GO:0090141   GO:0005886   GO:0060161   GO:1902950   GO:0099073   GO:0010629   GO:1990126   GO:0098978   GO:1903828   GO:0006624   GO:0032463   GO:0007040   GO:0098793   GO:0099003   GO:0048471   GO:1905166   GO:0014069   GO:1903181   GO:0043005   GO:0090263   GO:0098887   GO:0007416   GO:0061357   GO:0005768   GO:0050882   GO:0010821   GO:0033365   GO:0098794   GO:0043025   GO:0060548   GO:0005737   GO:0090326   GO:0031648   GO:0032268   GO:0008565   GO:0006428   GO:0004812   GO:0000049   GO:0005975   GO:0004553  

PANTHER

PTHR11099  

Subcellular location

Cytoplasm  
Membrane  
Endosome  
Early endosome  
Late endosome  

Length:

399

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.536520000000001

Exp number, first 60 AAs:

0.52703

Total prob of N-in:

0.03572

outside

1  -  399

 

 

Pi

188.535129

Theta

139.584759

Tajima's D

1.481

CLR

0.136577

CSRT

0.783560821958902

Interpretation

Uncertain