Gene

KWMTBOMO12199  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA014177

Annotation

PREDICTED:_26S_protease_regulatory_subunit_8_[Amyelois_transitella]

Location

Bomo_Chr20(+):9290690-9292033      View in EpiBrowser

Full name

26S proteasome regulatory subunit 8       + More
Ribosome biogenesis protein WDR12 homolog      
Putative rRNA methyltransferase      

Alternative Name

Protein 18-56
2'-O-ribose RNA methyltransferase SPB1 homolog

Location in the cell

Cytoplasmic   Reliability : 3.818

CDS

ATGACTCTCACGACTAAAATGGAAGTGGACACGGTGAAGGGTGAGGGTTTTCGCCCGTACTACATAACCAAGATTGAAGAGTTACAGCTCATTGTCGCCGAAAAATCTCAAAATCTTCGTCGTCTTCAAGCCCAGCGTAATGAACTCAACGCTAAAGTTCGTATGCTACGCGAGGAGCTTCAGCTCTTGCAAGAACAGGGATCGTACGTCGGAGAAGTGGTTAAACCAATGGATAAAAAGAAGGTCCTCGTAAAAGTTCATCCTGAAGGTAAATTCGTCGTCGATTTAGACAAGAACGTTGATATTAACGATGTCACGGCCAATTGTCGTGTCGCTCTTCGCAACGAAAGCTATACCTTACACAAAATACTACCCAACAAAGTCGATCCTCTTGTGTCGCTCATGATGGTCGAGAAAGTGCCGGACTCTACTTACGAAATGGTAGGTGGTCTAGACAAACAAATCAAGGAGATCAAAGAGGTGATTGAGCTGCCTGTGAAGCACCCAGAGCTGTTTGATGCCCTTGGGATTGCGCAACCAAAAGGAGTCTTACTGTATGGGCCTCCGGGCACTGGAAAGACATTATTAGCTCGTGCTGTCGCTCACCACACTGAGTGTACTTTCATACGTGTTTCTGGATCAGAATTGGTACAAAAATTTATTGGAGAAGGCAGCAGAATGGTGCGAGAGCTCTTCGTAATGGCCAGAGAACATGCTCCTTCTATCATTTTCATGGACGAAATAGACTCTATTGGGTCTTCCAGGATTGAGTCAGGAAGTGGGGGCGACTCTGAAGTTCAGAGAACCATGTTGGAGCTGCTGAATCAACTTGATGGTTTTGAAGCTACGAAAAACATCAAAGTCATTATGGCAACTAATAGAATTGATATCCTTGACCCTGCTTTGCTCCGACCCGGTCGTATTGATAGAAAAATTGAGTTCCCTCCGCCAAATGAGGAGGCCCGTTTGGACATCCTCAAAATTCACTCGAGAAAAATGAATCTGACTCGAGGAATCAATTTACGTAAGATTGCAGAGTTGATGCCAGGAGCTTCGGGTGCTGAAGTTAAGGGCGTTTGTACTGAGGCTGGAATGTATGCTCTGCGTGAGAGACGAGTGCACGTAACTCAGGAGGACTTTGAAATGGCTGTCGCTAAAGTCATGCAGAAGGACTCTGAAAAGAATATGTCCATCAAGAAACTTTGGAAGTGA

Protein

MTLTTKMEVDTVKGEGFRPYYITKIEELQLIVAEKSQNLRRLQAQRNELNAKVRMLREELQLLQEQGSYVGEVVKPMDKKKVLVKVHPEGKFVVDLDKNVDINDVTANCRVALRNESYTLHKILPNKVDPLVSLMMVEKVPDSTYEMVGGLDKQIKEIKEVIELPVKHPELFDALGIAQPKGVLLYGPPGTGKTLLARAVAHHTECTFIRVSGSELVQKFIGEGSRMVRELFVMAREHAPSIIFMDEIDSIGSSRIESGSGGDSEVQRTMLELLNQLDGFEATKNIKVIMATNRIDILDPALLRPGRIDRKIEFPPPNEEARLDILKIHSRKMNLTRGINLRKIAELMPGASGAEVKGVCTEAGMYALRERRVHVTQEDFEMAVAKVMQKDSEKNMSIKKLWK

Description

The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex (By similarity).
Required for maturation of ribosomal RNAs and formation of the large ribosomal subunit.
Probable methyltransferase involved in the maturation of rRNA and in the biogenesis of ribosomal subunits.

Catalytic Activity

a ribonucleotide in rRNA + S-adenosyl-L-methionine = a 2'-O-methylribonucleotide in rRNA + H(+) + S-adenosyl-L-homocysteine

Subunit

Component of the 19S proteasome regulatory particle complex. The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. Interacts with Rpn6 (PubMed:22187461). Interacts with imd and Usp2 isoform A (PubMed:25027767).

Similarity

Belongs to the AAA ATPase family.
Belongs to the WD repeat WDR12/YTM1 family.
Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family. SPB1 subfamily.

Keywords

ATP-binding   Cytoplasm   Nucleotide-binding   Nucleus   Proteasome   Complete proteome   Reference proteome  

Feature

chain  26S proteasome regulatory subunit 8

Uniprot

H9JXB2   A0A0L7KH57   P54814   S4NX72   I4DJY4   A0A2W1BBS8   + More
A0A2A4JDW3   A0A2H1WC68   A0A212FEY7   A0A0N0PB72   A0A023ETT7   A0A2A3ED22   A0A0B4J2N0   K7ITC8   Q16FL0   A0A232FNN7   A0A1L8DT48   T1E2P4   A0A0J7KCP0   E2B8P1   A0A1B0CCF5   A0A195DMW1   Q29I61   A0A195AWC2   A0A3L8DY13   A0A151WPJ5   A0A0K8UJX8   A0A0C9Q3V3   A0A0A1XDG7   W8BRU4   U5EMB1   A0A195EQ66   B3N0G6   B4N1I1   B0WSW1   A0A1I8MMH1   D6X1A1   A0A1I8Q7I4   A0A1L8EGU9   A0A1W4VWN2   A0A3B0KZD1   A0A1Y1K263   A0A0L0C3P0   V5GYP1   A0A1Q3FCW5   A0A1W4X9B7   A0A0C9R7B5   B4R3Q8   J3JW63   B4PZ23   B4I6G1   B3NYC7   B4JLM1   O18413   A0A182J2H3   B4M1V5   A0A0M4F8W0   A0A0K8TL55   A0A224XKX0   B4L8N1   A0A0P4VQP7   A0A023FAX2   A0A1B6HHN4   A0A069DYX0   R4G4V6   A0A182XLL4   A0A067RB45   A0A1B6M2L2   W5JVB2   A0A182JPN1   A0A2M3Z5A1   A0A1A9X8R8   A0A1A9VRK4   A0A1B0C6W9   A0A310SM55   A0A1A9ZGL4   A0A2M4ABB2   A0A182FCH3   D3TS21   A0A2M4BQC4   A0A3B0KRZ2   A0A2C9GRS5   A0A182TQ69   A0A084VQX0   Q7PTA8   A0A0A9YN60   A0A182LX01   A0A182NKR7   F4W633   A0A182T287   A0A182YMM9   A0A0K8TIN3   A0A182P251   A0A1I9WLJ8   A0A2J7QSC5   E0VCG2   A0A182URA9   A0A1S3D1I0   A0A182QK28   T1J8E5  

EC Number

2.1.1.-

Pubmed

19121390    26227816    8606008    23622113    22651552    26354079    + More
28756777    22118469    24945155    26483478    20075255    17510324    28648823    24330624    20798317    15632085    17994087    30249741    25830018    24495485    25315136    18362917    19820115    28004739    26108605    22516182    17550304    9469929    9738875    10731132    12537572    12537569    22187461    25027767    26369729    27129103    25474469    26334808    24845553    20920257    23761445    20353571    24438588    12364791    14747013    17210077    25401762    26823975    21719571    25244985    27538518    20566863   

EMBL

BABH01035721    JTDY01009801    KOB62647.1    U43728    GAIX01012277    JAA80283.1    + More
AK401602    KQ459461    KQ459053    BAM18224.1    KPJ00626.1    KPJ04220.1    KZ150467    PZC70777.1    NWSH01007661    NWSH01001818    PCG62874.1    PCG70029.1    ODYU01007671    SOQ50660.1    AGBW02008886    OWR52294.1    KQ461113    KPJ08909.1    JXUM01134081    GAPW01001764    KQ568069    JAC11834.1    KXJ69155.1    KZ288296    PBC28921.1    AAZX01003296    CH478417    CH478078    EAT33024.1    EAT34058.1    NNAY01000006    OXU32129.1    GFDF01004540    JAV09544.1    GALA01001110    JAA93742.1    LBMM01009368    KMQ88183.1    GL446361    EFN87947.1    AJWK01006689    KQ980724    KYN14161.1    CH379063    EAL32792.1    KQ976731    KYM76335.1    QOIP01000002    RLU25344.1    KQ982892    KYQ49615.1    GDHF01025320    JAI26994.1    GBYB01008738    JAG78505.1    GBXI01004858    JAD09434.1    GAMC01010588    GAMC01010587    GAMC01010584    JAB95971.1    GANO01001109    JAB58762.1    KQ982021    KYN30425.1    CH902640    EDV38370.1    CH963925    EDW78220.1    DS232077    EDS34089.1    KQ971372    EFA09518.2    GFDG01000852    JAV17947.1    OUUW01000021    SPP89438.1    GEZM01094987    JAV55559.1    JRES01000948    KNC26905.1    GALX01002908    JAB65558.1    GFDL01009621    JAV25424.1    GBYB01012214    JAG81981.1    CM000366    EDX18526.1    BT127481    AEE62443.1    CM000162    EDX03084.1    CH480823    EDW56367.1    CH954180    EDV47606.1    CH916370    EDW00474.1    U97538    AF043734    AE014298    AY051732    CH940651    EDW65659.1    CP012528    ALC48532.1    GDAI01002529    JAI15074.1    GFTR01005958    JAW10468.1    CH933815    EDW08006.1    GDKW01001253    JAI55342.1    GBBI01000533    JAC18179.1    GECU01033500    JAS74206.1    GBGD01001655    JAC87234.1    ACPB03019847    GAHY01001045    JAA76465.1    KK852575    KDR20973.1    GEBQ01009806    JAT30171.1    ADMH02000008    ETN68121.1    GGFM01002941    MBW23692.1    JXJN01027157    KQ759878    OAD62242.1    GGFK01004754    MBW38075.1    CCAG010005004    EZ424223    ADD20499.1    GGFJ01005990    MBW55131.1    SPP89439.1    APCN01000179    ATLV01015338    KE525006    KFB40364.1    AAAB01008807    EAA04200.3    GBHO01010543    GDHC01021934    JAG33061.1    JAP96694.1    AXCM01002371    GL887707    EGI70168.1    GBRD01000560    JAG65261.1    KU932382    APA34018.1    NEVH01011877    PNF31473.1    DS235053    EEB11068.1    AXCN02000542    JH431954   

Proteomes

UP000005204    UP000037510    UP000053268    UP000218220    UP000007151    UP000053240    + More
UP000069940    UP000249989    UP000242457    UP000005203    UP000002358    UP000008820    UP000215335    UP000036403    UP000008237    UP000092461    UP000078492    UP000001819    UP000078540    UP000279307    UP000075809    UP000078541    UP000007801    UP000007798    UP000002320    UP000095301    UP000007266    UP000095300    UP000192221    UP000268350    UP000037069    UP000192223    UP000000304    UP000002282    UP000001292    UP000008711    UP000001070    UP000000803    UP000075880    UP000008792    UP000092553    UP000009192    UP000015103    UP000076407    UP000027135    UP000000673    UP000075881    UP000092443    UP000078200    UP000092460    UP000092445    UP000069272    UP000092444    UP000075840    UP000075902    UP000030765    UP000007062    UP000075883    UP000075884    UP000007755    UP000075901    UP000076408    UP000075885    UP000235965    UP000009046    UP000075903    UP000079169    UP000075886   

PRIDE

H9JXB2    P54814    O18413   

Pfam

PF17862   AAA_lid_3        + More
PF00004   AAA
PF16450   Prot_ATP_ID_OB
PF16178   Anoct_dimer
PF04547   Anoctamin
PF00400   WD40
PF08154   NLE
PF01728   FtsJ
PF07780   Spb1_C
PF11861   DUF3381
PF01423   LSM
PF13869   NUDIX_2

Interpro

IPR003593   AAA+_ATPase        + More
IPR041569   AAA_lid_3       
IPR032501   Prot_ATP_ID_OB       
IPR005937   26S_Psome_P45-like       
IPR003959   ATPase_AAA_core       
IPR027417   P-loop_NTPase       
IPR003960   ATPase_AAA_CS       
IPR032394   Anoct_dimer       
IPR007632   Anoctamin       
IPR036322   WD40_repeat_dom_sf       
IPR019775   WD40_repeat_CS       
IPR028599   WDR12/Ytm1       
IPR012972   NLE       
IPR020472   G-protein_beta_WD-40_rep       
IPR001680   WD40_repeat       
IPR017986   WD40_repeat_dom       
IPR015943   WD40/YVTN_repeat-like_dom_sf       
IPR028589   Spb1-like       
IPR024576   rRNA_MeTfrase_Spb1_DUF3381       
IPR015507   rRNA-MeTfrase_E       
IPR002877   rRNA_MeTrfase_FtsJ_dom       
IPR012920   rRNA_MeTfrase_Spb1_C       
IPR029063   SAM-dependent_MTases       
IPR034102   Sm_D1       
IPR001163   LSM_dom_euk/arc       
IPR010920   LSM_dom_sf       
IPR016706   Cleav_polyA_spec_factor_su5       
IPR015797   NUDIX_hydrolase-like_dom_sf       
IPR000086   NUDIX_hydrolase_dom       

SUPFAM

SSF52540   SSF52540        + More
SSF50978   SSF50978       
SSF53335   SSF53335       
SSF50182   SSF50182       
SSF55811   SSF55811       

Gene 3D

2.130.10.10    

CDD

cd01724  Sm_D1

ProteinModelPortal

H9JXB2   A0A0L7KH57   P54814   S4NX72   I4DJY4   A0A2W1BBS8   + More
A0A2A4JDW3   A0A2H1WC68   A0A212FEY7   A0A0N0PB72   A0A023ETT7   A0A2A3ED22   A0A0B4J2N0   K7ITC8   Q16FL0   A0A232FNN7   A0A1L8DT48   T1E2P4   A0A0J7KCP0   E2B8P1   A0A1B0CCF5   A0A195DMW1   Q29I61   A0A195AWC2   A0A3L8DY13   A0A151WPJ5   A0A0K8UJX8   A0A0C9Q3V3   A0A0A1XDG7   W8BRU4   U5EMB1   A0A195EQ66   B3N0G6   B4N1I1   B0WSW1   A0A1I8MMH1   D6X1A1   A0A1I8Q7I4   A0A1L8EGU9   A0A1W4VWN2   A0A3B0KZD1   A0A1Y1K263   A0A0L0C3P0   V5GYP1   A0A1Q3FCW5   A0A1W4X9B7   A0A0C9R7B5   B4R3Q8   J3JW63   B4PZ23   B4I6G1   B3NYC7   B4JLM1   O18413   A0A182J2H3   B4M1V5   A0A0M4F8W0   A0A0K8TL55   A0A224XKX0   B4L8N1   A0A0P4VQP7   A0A023FAX2   A0A1B6HHN4   A0A069DYX0   R4G4V6   A0A182XLL4   A0A067RB45   A0A1B6M2L2   W5JVB2   A0A182JPN1   A0A2M3Z5A1   A0A1A9X8R8   A0A1A9VRK4   A0A1B0C6W9   A0A310SM55   A0A1A9ZGL4   A0A2M4ABB2   A0A182FCH3   D3TS21   A0A2M4BQC4   A0A3B0KRZ2   A0A2C9GRS5   A0A182TQ69   A0A084VQX0   Q7PTA8   A0A0A9YN60   A0A182LX01   A0A182NKR7   F4W633   A0A182T287   A0A182YMM9   A0A0K8TIN3   A0A182P251   A0A1I9WLJ8   A0A2J7QSC5   E0VCG2   A0A182URA9   A0A1S3D1I0   A0A182QK28   T1J8E5  

PDB

6EPF     E-value=0,     Score=1670

KEGG

101747034    K03066 26S proteasome regulatory subunit T6 | (RefSeq) 26S protease regulatory subunit 8

PATHWAY

03050    Proteasome - Bombyx mori (domestic silkworm)

GO

GO:0005737   GO:0036402   GO:0030163   GO:0005524   GO:0008233   GO:0000502   GO:0005634   GO:0008540   GO:0043161   GO:0031595   GO:0045899   GO:0017025   GO:0050804   GO:0098794   GO:0031597   GO:0005838   GO:0007623   GO:0005829   GO:0005654   GO:0008063   GO:0016887   GO:0016787   GO:0046983   GO:0016021   GO:0030687   GO:0005730   GO:0000463   GO:0000466   GO:0043021   GO:0000453   GO:0008649   GO:0000387   GO:0008568   GO:0003729   GO:0005849   GO:0006378   GO:0016788   GO:0007165   GO:0005525   GO:0008033   GO:0003676  

Subcellular location

Cytoplasm  
Nucleus  
Nucleolus  
Nucleoplasm  
  

Length:

403

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.00161

Exp number, first 60 AAs:

0

Total prob of N-in:

0.02541

outside

1  -  403

 

 

Pi

142.590596

Theta

148.21052

Tajima's D

-0.418101

CLR

0.594908

CSRT

0.259587020648968

Interpretation

Uncertain