Gene

KWMTBOMO12176  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA004227

Annotation

flap_endonuclease-1_[Bombyx_mori]

Location

Bomo_Chr20(+):8802810-8808463      View in EpiBrowser

Full name

Flap endonuclease 1      

Alternative Name

Flap structure-specific endonuclease 1

Location in the cell

Cytoplasmic   Reliability : 3.031

CDS

ATGGGTATTCTAGGTTTATCTAAGTTAATTGCTGACATAGCACCTCATGCTGTCAAAGAAATGGAAATCAAAAATTACTTTGGAAGAAAAATAGCTATTGATGCTTCTATGAGCTTATATCAGTTCCTTATCGCCGTAAGAAGTGAAGGAGCCCAACTAGTCTCTGTAGACGGAGAGACTACATCACATCTGATGGGTACATTTTACCGTACTATAAGACTGGTAGAAAATGGGATTAAACCAGTGTATGTCTTTGACGGGAAACCTCCAGACATGAAGGCTCATCAGTTGAATAAGAGAGCAGAAAGGAGAGAGGAAGCAGAGAAAGAATTGCAAAAAGCTACTGAAGCCGGTGACACAGCTTCAGTGGACAAGTTCAACAGACGTTTGGTTAAAGTAACAAAACAACACAGTGAAGAGGCTAAACAACTCCTCAAATTGATGGGTGTCCCGGTTGTTGATGCACCCTGTGAGGCTGAAGCCCAGTGTGCAGCTCTTGTAAAATCCGGTAAAGTTTTTGCAGCAGCCACAGAAGATATGGATGCCCTAACCTTTGGTGCACCAGTGCTATTGAGACACTTAACGTTTTCTGAAGCAAGGAAAATGCCCGTGCAAGAATTCCATTTAAATAATGTACTACAGGGATTGGAATTGAAACAGAATGAGTTTATTGACCTCTGCATACTCCTTGGCTGTGATTACTGTGGTTCCATTAGAGGGGTTGGCCCAAAACGAGCCATCGACCTCATAAGACAACATCGGACACTGGATGAAGTACTCAAGAATATAGACACTGAGAAGTACCAACCTCCCACTGACTGGGATTACGAGCGAGCTAGGAGTCTGTTCATGGAACCTGAAGTCGCTGACCCTAAAGACATTGAGCTGAAATGGACTGATCCCGATGAAGAGGGCCTCGTGAAGTTTCTGTGTGGGGACAGACAGTTCAATGAAGAACGAGTGAGGAATGGAGCTAAGAAGTTAATTAAAGCCAGGACGGGCACCACACAGGCCAGATTAGATGGGTTCTTCACGTTAACGACAACGCCTAATTCGAAACGTAAAGCTGAAGAGGACAAGAAGAATTCAGCAAAGAAGAAAACTAAGACCGGAGGTGGAGGACGCGGTCGGAAACCGAAATGA

Protein

MGILGLSKLIADIAPHAVKEMEIKNYFGRKIAIDASMSLYQFLIAVRSEGAQLVSVDGETTSHLMGTFYRTIRLVENGIKPVYVFDGKPPDMKAHQLNKRAERREEAEKELQKATEAGDTASVDKFNRRLVKVTKQHSEEAKQLLKLMGVPVVDAPCEAEAQCAALVKSGKVFAAATEDMDALTFGAPVLLRHLTFSEARKMPVQEFHLNNVLQGLELKQNEFIDLCILLGCDYCGSIRGVGPKRAIDLIRQHRTLDEVLKNIDTEKYQPPTDWDYERARSLFMEPEVADPKDIELKWTDPDEEGLVKFLCGDRQFNEERVRNGAKKLIKARTGTTQARLDGFFTLTTTPNSKRKAEEDKKNSAKKKTKTGGGGRGRKPK

Description

Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.

Cofactor

Mg(2+)

Subunit

Interacts with PCNA. Three molecules of 101741812 bind to one PCNA trimer with each molecule binding to one PCNA monomer. PCNA stimulates the nuclease activity without altering cleavage specificity.
Interacts with PCNA. Three molecules of Fen1 bind to one PCNA trimer with each molecule binding to one PCNA monomer. PCNA stimulates the nuclease activity without altering cleavage specificity.
Interacts with PCNA. Three molecules of Fen1_1 bind to one PCNA trimer with each molecule binding to one PCNA monomer. PCNA stimulates the nuclease activity without altering cleavage specificity.
Interacts with PCNA. Three molecules of FEN1 bind to one PCNA trimer with each molecule binding to one PCNA monomer. PCNA stimulates the nuclease activity without altering cleavage specificity.
Interacts with PCNA. Three molecules of 100160968 bind to one PCNA trimer with each molecule binding to one PCNA monomer. PCNA stimulates the nuclease activity without altering cleavage specificity.
Interacts with PCNA. Three molecules of 109401579 bind to one PCNA trimer with each molecule binding to one PCNA monomer. PCNA stimulates the nuclease activity without altering cleavage specificity.
Interacts with PCNA. Three molecules of LOC108744458 bind to one PCNA trimer with each molecule binding to one PCNA monomer. PCNA stimulates the nuclease activity without altering cleavage specificity.
Interacts with PCNA. Three molecules of CSON014361 bind to one PCNA trimer with each molecule binding to one PCNA monomer. PCNA stimulates the nuclease activity without altering cleavage specificity.
Interacts with PCNA. Three molecules of CSON009372 bind to one PCNA trimer with each molecule binding to one PCNA monomer. PCNA stimulates the nuclease activity without altering cleavage specificity.
Interacts with PCNA. Three molecules of 109542723 bind to one PCNA trimer with each molecule binding to one PCNA monomer. PCNA stimulates the nuclease activity without altering cleavage specificity.
Interacts with PCNA. Three molecules of HaOG214678 bind to one PCNA trimer with each molecule binding to one PCNA monomer. PCNA stimulates the nuclease activity without altering cleavage specificity.
Interacts with PCNA. Three molecules of LOC412308 bind to one PCNA trimer with each molecule binding to one PCNA monomer. PCNA stimulates the nuclease activity without altering cleavage specificity.
Interacts with PCNA. Three molecules of 100114897 bind to one PCNA trimer with each molecule binding to one PCNA monomer. PCNA stimulates the nuclease activity without altering cleavage specificity.
Interacts with PCNA. Three molecules of putative Flap endonuclease 1 bind to one PCNA trimer with each molecule binding to one PCNA monomer. PCNA stimulates the nuclease activity without altering cleavage specificity.
Interacts with PCNA. Three molecules of 101891979 bind to one PCNA trimer with each molecule binding to one PCNA monomer. PCNA stimulates the nuclease activity without altering cleavage specificity.
Interacts with PCNA. Three molecules of 8232021 bind to one PCNA trimer with each molecule binding to one PCNA monomer. PCNA stimulates the nuclease activity without altering cleavage specificity.
Interacts with PCNA. Three molecules of Fen1_0 bind to one PCNA trimer with each molecule binding to one PCNA monomer. PCNA stimulates the nuclease activity without altering cleavage specificity.
Interacts with PCNA. Three molecules of DsimGD25512 bind to one PCNA trimer with each molecule binding to one PCNA monomer. PCNA stimulates the nuclease activity without altering cleavage specificity.
Interacts with PCNA. Three molecules of LOC108090516 bind to one PCNA trimer with each molecule binding to one PCNA monomer. PCNA stimulates the nuclease activity without altering cleavage specificity.

Similarity

Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.

Keywords

Complete proteome   DNA damage   DNA repair   DNA replication   Endonuclease   Exonuclease   Hydrolase   Magnesium   Metal-binding   Mitochondrion   Nuclease   Nucleus   Phosphoprotein   Reference proteome  

Feature

chain  Flap endonuclease 1

Uniprot

H9J3Y8   E4W6M7   A0A2A4IZZ0   A0A2H1V8V8   S4PEV2   A0A194QFL0   + More
A0A212FCF5   A0A0A9VS25   A0A2J7PL47   A0A1Y1NBN2   A0A2J7PL71   A0A2H8U0C0   A0A2S2QJB4   Q178M1   D6WSD4   J9JPN9   A0A182GK55   T1I6X2   A0A1W4XSG4   A0A023F5C2   A0A067REI0   F4WVU6   A0A195F9L4   A0A1Q3FVN5   E2B3F3   A0A158NF76   U5EXV5   A0A195BT57   A0A336KU20   A0A151IMV1   B4LM90   A0A1I8NVM8   A0A0J7P3N5   A0A182WF49   A0A151XIE0   A0A182MFJ3   A0A336LGJ2   B4J6M4   A0A310SKE1   J3JZD2   A0A026WW32   A0A182QGV0   A0A2W1B8V7   A0A2M4ANS6   A0A087ZNQ8   W5JNU7   A0A0L7R2F5   E2AQ57   A0A182RFB4   A0A182MXX3   A0A084WBF5   A0A182HNL3   A0A182FTC7   A0A2A3EUD0   A0A182TUI8   K7J3I0   A0A2M4ANW6   A0A182VA17   A0A182LPJ9   A0A182XCB2   Q7Q323   A0A154P9X1   A0A2M4BQW6   A0A1J1IWP0   B4KNM1   A0A151IR65   A0A1I8MCS0   A0A182K239   A0A182P9D7   W8B4E0   E0VP82   A0A182JC21   A0A0P4W461   A0A232FFN0   A0A3B0JM56   A0A034VMU5   A0A0C9QKM1   B5DUR8   B4GIM3   B4P5U9   V4A3E8   B3MDA3   A0A0K8U070   Q7K7A9   A0A0M8ZV55   B3NP61   A0A0J9REG4   A7RRJ0   B4QIG6   A0A1A9XP19   A0A1A9ZGI0   A0A1A9VLL7   B4HTA1   A0A2M4BR82   A0A182YDD2   D3TQJ5   A0A1W4UZQ2   A0A0P5FZQ9   A0A1B6LSG2   A0A0P5V9E9  

EC Number

3.1.-.-

Pubmed

19121390    21612397    23622113    26354079    22118469    25401762    + More
26823975    28004739    17510324    18362917    19820115    26483478    25474469    24845553    21719571    20798317    21347285    17994087    22516182    23537049    24508170    30249741    28756777    20920257    23761445    24438588    20075255    20966253    12364791    25315136    24495485    20566863    28648823    25348373    15632085    23254933    10731132    12537572    10731137    22936249    17615350    25244985    20353571   

EMBL

BABH01040234    FJ844436    ACY92094.1    NWSH01004790    PCG64683.1    ODYU01001261    + More
SOQ37231.1    GAIX01004327    JAA88233.1    KQ459053    KPJ04199.1    AGBW02009194    OWR51426.1    GBHO01045055    GBRD01011951    GDHC01020956    JAF98548.1    JAG53873.1    JAP97672.1    NEVH01024527    PNF17057.1    GEZM01007136    JAV95354.1    PNF17059.1    GFXV01008178    MBW19983.1    GGMS01008407    MBY77610.1    CH477362    KQ971352    EFA06383.1    ABLF02010587    JXUM01069301    KQ562544    KXJ75657.1    ACPB03005124    GBBI01002398    JAC16314.1    KK852513    KDR22281.1    GL888398    EGI61676.1    KQ981720    KYN37295.1    GFDL01003374    JAV31671.1    GL445323    EFN89831.1    ADTU01013894    GANO01000755    JAB59116.1    KQ976417    KYM89616.1    UFQS01000794    UFQT01000794    SSX06945.1    SSX27289.1    KQ977019    KYN06276.1    CH940648    LBMM01000187    KMR04549.1    KQ982080    KYQ60174.1    AXCM01000772    UFQS01003718    UFQT01003718    SSX15877.1    SSX35220.1    CH916367    KQ765071    OAD54145.1    APGK01052259    BT128613    KB741211    KB632263    AEE63570.1    ENN72811.1    ERL90946.1    KK107109    QOIP01000008    EZA59309.1    RLU19537.1    AXCN02002114    KZ150433    PZC70885.1    GGFK01009125    MBW42446.1    ADMH02000540    ETN66057.1    KQ414666    KOC65019.1    GL441701    EFN64391.1    ATLV01022351    KE525331    KFB47549.1    APCN01001943    KZ288186    PBC34739.1    GGFK01009126    MBW42447.1    AAAB01008966    KQ434839    KZC08018.1    GGFJ01006334    MBW55475.1    CVRI01000063    CRL04575.1    CH933808    KQ981145    KYN09135.1    GAMC01010575    JAB95980.1    AAZO01004118    DS235357    EEB15188.1    GDRN01086084    JAI61239.1    NNAY01000337    OXU29107.1    OUUW01000001    SPP74629.1    GAKP01016084    JAC42868.1    GBYB01001137    JAG70904.1    CH674335    CH479183    CM000158    KB201262    ESO98353.1    CH902619    GDHF01032604    GDHF01026005    JAI19710.1    JAI26309.1    AE013599    AL031863    BT100031    KQ435876    KOX70179.1    CH954179    CM002911    KMY94377.1    DS469531    CM000362    CH480816    GGFJ01006456    MBW55597.1    EZ423697    GDIQ01253391    JAJ98333.1    GEBQ01013403    JAT26574.1    GDIP01102844    JAM00871.1   

Proteomes

UP000005204    UP000218220    UP000053268    UP000007151    UP000235965    UP000008820    + More
UP000007266    UP000007819    UP000069940    UP000249989    UP000015103    UP000192223    UP000027135    UP000007755    UP000078541    UP000008237    UP000005205    UP000078540    UP000078542    UP000008792    UP000095300    UP000036403    UP000075920    UP000075809    UP000075883    UP000001070    UP000019118    UP000030742    UP000053097    UP000279307    UP000075886    UP000005203    UP000000673    UP000053825    UP000000311    UP000075900    UP000075884    UP000030765    UP000075840    UP000069272    UP000242457    UP000075902    UP000002358    UP000075903    UP000075882    UP000076407    UP000007062    UP000076502    UP000183832    UP000009192    UP000078492    UP000095301    UP000075881    UP000075885    UP000009046    UP000075880    UP000215335    UP000268350    UP000001819    UP000008744    UP000002282    UP000030746    UP000007801    UP000000803    UP000053105    UP000008711    UP000001593    UP000000304    UP000092443    UP000092445    UP000078200    UP000001292    UP000076408    UP000092444    UP000192221   

PRIDE

H9J3Y8    E4W6M7    Q178M1    B4J6M4    Q7Q323    B4KNM1    + More
Q7K7A9    D3TQJ5   

Pfam

PF00752   XPG_N        + More
PF00867   XPG_I

Interpro

IPR006085   XPG_DNA_repair_N        + More
IPR023426   Flap_endonuc       
IPR036279   5-3_exonuclease_C_sf       
IPR029060   PIN-like_dom_sf       
IPR006086   XPG-I_dom       
IPR019974   XPG_CS       
IPR006084   XPG/Rad2       
IPR008918   HhH2       
IPR002421   5-3_exonuclease_N       

SUPFAM

SSF88723   SSF88723        + More
SSF47807   SSF47807       

CDD

cd09867  PIN_FEN1

ProteinModelPortal

H9J3Y8   E4W6M7   A0A2A4IZZ0   A0A2H1V8V8   S4PEV2   A0A194QFL0   + More
A0A212FCF5   A0A0A9VS25   A0A2J7PL47   A0A1Y1NBN2   A0A2J7PL71   A0A2H8U0C0   A0A2S2QJB4   Q178M1   D6WSD4   J9JPN9   A0A182GK55   T1I6X2   A0A1W4XSG4   A0A023F5C2   A0A067REI0   F4WVU6   A0A195F9L4   A0A1Q3FVN5   E2B3F3   A0A158NF76   U5EXV5   A0A195BT57   A0A336KU20   A0A151IMV1   B4LM90   A0A1I8NVM8   A0A0J7P3N5   A0A182WF49   A0A151XIE0   A0A182MFJ3   A0A336LGJ2   B4J6M4   A0A310SKE1   J3JZD2   A0A026WW32   A0A182QGV0   A0A2W1B8V7   A0A2M4ANS6   A0A087ZNQ8   W5JNU7   A0A0L7R2F5   E2AQ57   A0A182RFB4   A0A182MXX3   A0A084WBF5   A0A182HNL3   A0A182FTC7   A0A2A3EUD0   A0A182TUI8   K7J3I0   A0A2M4ANW6   A0A182VA17   A0A182LPJ9   A0A182XCB2   Q7Q323   A0A154P9X1   A0A2M4BQW6   A0A1J1IWP0   B4KNM1   A0A151IR65   A0A1I8MCS0   A0A182K239   A0A182P9D7   W8B4E0   E0VP82   A0A182JC21   A0A0P4W461   A0A232FFN0   A0A3B0JM56   A0A034VMU5   A0A0C9QKM1   B5DUR8   B4GIM3   B4P5U9   V4A3E8   B3MDA3   A0A0K8U070   Q7K7A9   A0A0M8ZV55   B3NP61   A0A0J9REG4   A7RRJ0   B4QIG6   A0A1A9XP19   A0A1A9ZGI0   A0A1A9VLL7   B4HTA1   A0A2M4BR82   A0A182YDD2   D3TQJ5   A0A1W4UZQ2   A0A0P5FZQ9   A0A1B6LSG2   A0A0P5V9E9  

PDB

1UL1     E-value=8.13901e-128,     Score=1170

KEGG

101741812    K04799 flap endonuclease-1 [EC:3.-.-.-] | (RefSeq) flap endonuclease 1

PATHWAY

03030    DNA replication - Bombyx mori (domestic silkworm)
03410    Base excision repair - Bombyx mori (domestic silkworm)
03450    Non-homologous end-joining - Bombyx mori (domestic silkworm)

GO

GO:0005739   GO:0005654   GO:0043137   GO:0000287   GO:0005730   GO:0003677   GO:0006260   GO:0006284   GO:0017108   GO:0008409   GO:0006281   GO:0004519   GO:0030145   GO:0004523   GO:0048256   GO:0005634   GO:0004527   GO:0004518   GO:0003824   GO:0016788   GO:0007165   GO:0005525   GO:0008033   GO:0003676   GO:0016787  

PANTHER

PTHR11081  

Subcellular location

Mitochondrion   Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.   With evidence from 1 publications.
Nucleus   Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.   With evidence from 1 publications.
Nucleolus   Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.   With evidence from 1 publications.
Nucleoplasm   Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.   With evidence from 1 publications.

Length:

380

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.00591

Exp number, first 60 AAs:

0.00383

Total prob of N-in:

0.00183

outside

1  -  380

 

 

Pi

194.472224

Theta

161.164295

Tajima's D

0.85523

CLR

0.152995

CSRT

0.626218689065547

Interpretation

Uncertain