SGID Silkworm Genome Informatics Database
Gene
KWMTBOMO11590  Validated by peptides from experiments
Pre Gene Modal
BGIBMGA004019
Annotation
X-ray_repair_cross-complementing_protein_5_[Operophtera_brumata]
Full name
X-ray repair cross-complementing protein 5       + More
ATP-dependent DNA helicase 2 subunit 1      
Alternative Name
5'-deoxyribose-5-phosphate lyase Ku70
ATP-dependent DNA helicase 2 subunit 1
ATP-dependent DNA helicase II 70 kDa subunit
DNA repair protein XRCC6
Ku autoantigen protein p70 homolog
ATP-dependent DNA helicase II subunit Ku70
Location in the cell
Nuclear   Reliability : 2.971
 

Sequence

CDS
ATGGATTCTGATATTGAAGTAGAAGAGTGCGAAGAGTTTTCTTATAGAGGAAACGCTGGAACTATAATTCTTATCAATGTATACGATCCCCTATCGTGTAAATTCCCACAAATTGCGCATGTTGCAACGTGTCAGGCACTCAAACATTATCTACGTACATCTACTTCGCATAATGTAGGTGTTGTCTTATATGGTATTGATGATCCAACTTCGAACATTAAAAACGTATTAGAAGTTATGCCATTGGCGCCACCTAACATGGATGACTATAAAAAACTAAAAAACATCAACATTCTAGCTCTCAAACAAGCAAAAGAATTAAGATTGTCAGATGCGTTATGGTACTGCAATAAAATGTTTAATAGTTGTACAAAAACACTATCCTCTCAAACTATTATTCTATTGTCAAGACTTGATACACCACCTCTGTCTGAGGATGAAACACCAACATTTGATAGAATTGTTGAATTGAATAATTCTGAGATAATTCTCAAATTAATTAATTTGTCTGACTCTGAATATGAAATTCATCAATTTTATAAAGATTTGCTATTTGAGGTAAATAAAAACTCATTGCCAAAATCAGTTTGGAAAATTGAAGATGTTACAAAGTTAATTCTAAATGAATGTGACCGTCATTTAGCGACCGCTCAGCTTATTTTTGAAATTGGGAATAATGTATCAATTGGTATTTCTATCTATAAACTATTGAAAAGCAACATAGAACCAAAGAAAGTGTATTTAAGTAATGAAACAAATGCTGTTGTAACTAGTGACACTAAAACGACTAAAGTTTTGGTAAAACCTGATACTGATATGGATATTGATAGGGTTGAAGAACAAGAAATGCCTTTACTTAAGTCTGAGCTGCTTCACTGTCAAGAATATGGCAATGAGATTATAGCATTCACTGATAATGAATTTAAAACTATAAATAACCCATTTGGTCCAGCGAAACTAAAACTGTTGGGATTCAAACCAGAAACTTTCCTCTGTAAAGAGAAATGGTTTTTGAAAAATTGTTCTTTCTTATTCCCTAATGAAAAATCAATAGAAGGTTCTACAACTGCTCTCAAGGCAATGCACCAAGCATGTATTGAAACAAAAACTGTAGCTATTTGTGTGATTTGTTCTAGAATAAATTCTAAACCTAACATAGTGGCTCTTTCACCCTGTACTCGGCCCCTTGGTTTAGATATAGATATTGGATTTGATGTCATACCATTACCATTTTCAGAAAATGTAAGGGATCTCTCAAGTCTTTTTTCAGATGAAATAGCAGATACGTCAGACACACAAAAAGATATGCTCAAAAATATTATTAATAATGTAAAGTTTAATTACACTCCAAGCATGTTTGAAAATCCTAAGTTGCAATCATTGTATAGAGTTATTGAAGCAAAAGCATTAAAACAAGATGACATAGAGCCTTTTGTTGATACCACAAAACCACTAAATAAAATATTTAATGAGATTGATGGGGAACAATTTTATGAACTATTTGGTCCATTCGGAGTAACCGCAGTCAAAAGAAATAGTGAAACAAAACCAGCTAATACCAAACTACAAAAGACTGCTGATGTCAATATTGATGTATTAGATCAAAGAGTTAAAGACAGAAAAGTAAATATGTATACTGTACCCCAACTTAAAGATATACTGAAACATAAGAAAGCAACAGAAGCTCTGACAGGTTTAAATAAAAGTAATCTAGTTGATTTAGTTTACAAATATTGTAGTTAA
Protein
MDSDIEVEECEEFSYRGNAGTIILINVYDPLSCKFPQIAHVATCQALKHYLRTSTSHNVGVVLYGIDDPTSNIKNVLEVMPLAPPNMDDYKKLKNINILALKQAKELRLSDALWYCNKMFNSCTKTLSSQTIILLSRLDTPPLSEDETPTFDRIVELNNSEIILKLINLSDSEYEIHQFYKDLLFEVNKNSLPKSVWKIEDVTKLILNECDRHLATAQLIFEIGNNVSIGISIYKLLKSNIEPKKVYLSNETNAVVTSDTKTTKVLVKPDTDMDIDRVEEQEMPLLKSELLHCQEYGNEIIAFTDNEFKTINNPFGPAKLKLLGFKPETFLCKEKWFLKNCSFLFPNEKSIEGSTTALKAMHQACIETKTVAICVICSRINSKPNIVALSPCTRPLGLDIDIGFDVIPLPFSENVRDLSSLFSDEIADTSDTQKDMLKNIINNVKFNYTPSMFENPKLQSLYRVIEAKALKQDDIEPFVDTTKPLNKIFNEIDGEQFYELFGPFGVTAVKRNSETKPANTKLQKTADVNIDVLDQRVKDRKVNMYTVPQLKDILKHKKATEALTGLNKSNLVDLVYKYCS

Summary

Description
Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription (By similarity).
Single-stranded DNA-dependent ATP-dependent helicase. Involved in non-homologous end joining (NHEJ) DNA double strand break repair. DNA-binding is sequence-independent but has a high affinity to nicks in double-stranded DNA and to the ends of duplex DNA. Binds to naturally occurring chromosomal ends, and therefore provides chromosomal end protection. Required also for telomere recombination to repair telomeric ends in the absence of telomerase. KU70, of the KU70/KU80 heterodimer, binds to the stem loop of TLC1, the RNA component of telomerase. Involved in telomere maintenance. Interacts with telomeric repeats and subtelomeric sequences thereby controlling telomere length and protecting against subtelomeric rearrangement. Maintains telomeric chromatin, which is involved in silencing the expression of genes located at the telomere. Required for mating-type switching (By similarity).
Catalytic Activity
ATP + H2O = ADP + H(+) + phosphate
Subunit
Heterodimer composed of XRCC5/Ku80 and XRCC6/Ku70. The dimer associates in a DNA-dependent manner with PRKDC to form the DNA-dependent protein kinase complex DNA-PK, and with the LIG4-XRCC4 complex to form the core of the non-homologous end joining (NHEJ) complex. Additional components of the NHEJ complex include NHEJ1/XLF and PAXX.
Heterodimer of a 70 kDa and a 80 kDa subunit.
Similarity
Belongs to the ku70 family.
Keywords
Activator   ATP-binding   Chromosome   Complete proteome   DNA damage   DNA recombination   DNA repair   DNA-binding   Helicase   Hydrolase   Lyase   Multifunctional enzyme   Nucleotide-binding   Nucleus   Phosphoprotein   Reference proteome   Transcription   Transcription regulation  
Feature
chain  X-ray repair cross-complementing protein 5
EC Number
3.6.4.12
EMBL
BABH01021571    NWSH01001566    PCG70842.1    JTDY01000700    KOB76169.1    GAIX01003873    + More
JAA88687.1    ODYU01013106    SOQ59792.1    GDQN01011174    JAT79880.1    KQ461073    KPJ09611.1    KQ458556    KPJ05822.1    KQ459185    KPJ03302.1    FX985843    BBA93730.1    HACA01020452    CDW37813.1    PZQS01000003    PVD33438.1    KB201304    ESO97963.1    CVRI01000057    CRL02262.1    NEDP02005356    OWF41790.1    KJ152435    AIL94177.1    KK852727    KDR17626.1    NEVH01020945    PNF20398.1    AAAB01008859    EAA08137.5    EGK96800.1    AAMC01009104    AAMC01009105    AAMC01009106    AAMC01009107    AAMC01009108    AAMC01009109    BC075271    AAH75271.1    APCN01000256    KJ401341    AIA61437.1    AB020608    BAA76953.1    AMQN01000816    KB296161    ELU12174.1    CM004472    OCT85332.1    GG666573    EEN53576.1    JXUM01098668    JXUM01098669    JXUM01098670    JXUM01098671    JXUM01098672    KQ564439    KXJ72268.1    AXCN02001469    BC077445    AAH77445.1    GDRN01084100    GDRN01084099    JAI61554.1    JH817518    EKC34975.1    AXCM01000275    KB481965    EMP41869.1    GDUN01000253    JAN95666.1    IACF01001655    LAB67344.1    AMQM01005815    KB097106    ESN99570.1    AB016529    KL217504    KFO96069.1    GANP01007026    JAB77442.1    ADBJ01000037    EFA79065.1    AADN05000523    GEFM01003488    JAP72308.1    KK943949    KFQ52863.1    LMAW01002623    KQK78918.1    HAAD01005146    CDG71378.1    IAAA01016318    IAAA01016319    LAA04412.1    KB376364    EMC89970.1    AKCR02000009    PKK29805.1    GFDF01003125    JAV10959.1    DS985265    EDV19898.1    UFQS01000590    UFQT01000590    SSX05181.1    SSX25542.1    ADON01129801    NOWV01000091    RDD40807.1    REGN01000530    RNA41208.1    KL379146    KFP86069.1    GFDL01012939    JAV22106.1    AKHW03002956    KYO36240.1    JH431836    GBEX01004021    JAI10539.1    GEBQ01032262    JAT07715.1    IACJ01046988    LAA40961.1    IACK01100981    IACK01100982    LAA82779.1    KK799808    KFQ27941.1    IACI01064708    LAA27229.1    AEFK01203879    AEFK01203880    AEFK01203881    JW866936    AFO99453.1    IACN01113340    LAB64905.1    GEDV01007804    JAP80753.1    L41356    KZ505643    PKU48975.1    AGTO01000068    IACN01113339    LAB64902.1    LSMT01000143    PFX25750.1   
Pfam
PF02735   Ku        + More
PF03731   Ku_N
PF03730   Ku_C
PF12949   HeH
PF02037   SAP
PF10283   zf-CCHH
Interpro
IPR016194   SPOC-like_C_dom_sf        + More
IPR006165   Ku70       
IPR005161   Ku_N       
IPR006164   Ku70/Ku80_beta-barrel_dom       
IPR027388   Ku70_bridge/pillars_dom_sf       
IPR036465   vWFA_dom_sf       
IPR005160   Ku_C       
IPR025856   HeH/LEM_domain       
IPR036361   SAP_dom_sf       
IPR002035   VWF_A       
IPR003034   SAP_dom       
IPR019406   APLF_PBZ       
IPR036779   LysM_dom_sf       
IPR018392   LysM_dom       
SUPFAM
SSF53300   SSF53300        + More
SSF100939   SSF100939       
SSF68906   SSF68906       
PDB
1JEY     E-value=1.1881e-30,     Score=334

Ontologies

Topology

Subcellular location
Nucleus  
Chromosome  
Length:
580
Number of predicted TMHs:
0
Exp number of AAs in TMHs:
0.0577000000000002
Exp number, first 60 AAs:
0.0532900000000001
Total prob of N-in:
0.00365
outside
1  -  580
 
 

Population Genetic Test Statistics

Pi
118.559613
Theta
162.005104
Tajima's D
-0.610671
CLR
0.253096
CSRT
0.215689215539223
Interpretation
Uncertain
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