Gene

KWMTBOMO11573

Pre Gene Modal

BGIBMGA004028

Annotation

PREDICTED:_testis-specific_serine/threonine-protein_kinase_3-like_isoform_X1_[Papilio_machaon]

Location

Bomo_Chr19(-):8713432-8716387      View in EpiBrowser

Full name

Testis-specific serine/threonine-protein kinase 4      

Location in the cell

Cytoplasmic   Reliability : 2.652

CDS

ATGCCGGGTCCAGATGTTCGCACTCATGACAATGTTCTCGGCGAGATGTCAGCAGAGCCTACGGCTACGGTACATAGCGGTGTGGAAGCCAAAACTGAAAGGAAACTGACGGTTCTTGAAAGCCACGGTTACATGCTTGGCAGAACAATTGGGTCCGGTTCCTATGCAACTGTTAAGGTGGCCTCTAGCGACAGACATAATTGTCAAGTTGCTATTAAAATAATCAGTAAATTTCAAGCCCCTGGTGATTATTTAAAAAAGTTCTTACCTAGAGAAATCGAAGTAGTTAAAGGCTTAAAGCATGAAAACTTAATACGTTTTCTTCAAGCAATAGAAACCACACATAGGGTTTACATAGTAATGGAATACGCCGAGAACGGGAGCCTGTTAGACATTATACGAAAAGATCAGCACATCGATGAGACCCGTGGACGTCGCTGGTTCAAGCAGCTGGTCGAAGCTGTCGATTACTGTCACGAACGTGGTGTTGTTCATAGGGACATCAAATGCGAAAATCTCCTGATGGATCACGGCTTGAACATAAAACTTTCCGATTTTGGCTTTGCTCGAGGGCATATGAAGCCGAAAAACGGAGTTTTCGCCTTAAGCGAGACATTTTGTGGGAGTTACGCATATGCGTCACCCGAAATTTTAAAAGGCGTTCCGTACAGGCCACAGGATTCTGATGTTTGGAGTATGGGAGTAGTATTATATGCCATCGTCTACGGACGTCTCCCGTTCGACGACACCAATTACACACAATTACTCAAGCAAGTTCAAAATAAAGTATCATTTCCCCGTGAACCTAAAGTGTCTGCGGAGTGTAGGAAACTTATAACTAGGATACTAGCCCCATTAAAAATGCGAGTAAAGATCCCTCAGATTCTAGCGGATCCATGGCTCAACCCCAACCAGCAATCTAAACATGACGAAACGGATTTACAAGAACACGCGAAAACCAGCAAAGAAGAGATAAAAAGTGTCAACATCGGAACCGTAACGTACGATAGAAAATTGGAAGAAGTTAAATAA

Protein

MPGPDVRTHDNVLGEMSAEPTATVHSGVEAKTERKLTVLESHGYMLGRTIGSGSYATVKVASSDRHNCQVAIKIISKFQAPGDYLKKFLPREIEVVKGLKHENLIRFLQAIETTHRVYIVMEYAENGSLLDIIRKDQHIDETRGRRWFKQLVEAVDYCHERGVVHRDIKCENLLMDHGLNIKLSDFGFARGHMKPKNGVFALSETFCGSYAYASPEILKGVPYRPQDSDVWSMGVVLYAIVYGRLPFDDTNYTQLLKQVQNKVSFPREPKVSAECRKLITRILAPLKMRVKIPQILADPWLNPNQQSKHDETDLQEHAKTSKEEIKSVNIGTVTYDRKLEEVK

Description

Isoform 1: Serine/threonine kinase which is involved in male germ cell development and in mature sperm function (PubMed:17927909, PubMed:23599433, PubMed:23054012, PubMed:25361759, PubMed:26940607). May be involved in the Cre/Creb signaling pathway (PubMed:26940607). Phosphorylates CREB1 on 'Ser-133' in vitro and can stimulate Cre/Creb pathway in cells (By similarity). Phosphorylates CREM on 'Ser-116' in vitro (PubMed:26940607). Phosphorylates ODF2 on 'Ser-95' (PubMed:26961893).
Isoform 2: Catalytically inactive.
Isoform 3: Catalytically inactive.
Isoform 4: Catalytically inactive.

Catalytic Activity

ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein]
ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein]

Cofactor

Mg(2+)

Subunit

Homodimer (PubMed:17927909, PubMed:23054012). Interacts with HSP90; this interaction stabilizes and activates TSSK4 (PubMed:23599433). Interacts with ODF2 (via C-terminus); this interaction promotes ODF2 phosphorylation on 'Ser-95' (PubMed:25361759, PubMed:26961893). May interact with CREM (PubMed:26940607). Interacts with CREB1; this interaction facilitates CREB1 phosphorylation on 'Ser-133' (By similarity).

Similarity

Belongs to the protein kinase superfamily.
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.

Keywords

Alternative splicing   ATP-binding   Cell projection   Cilium   Complete proteome   Cytoplasmic vesicle   Developmental protein   Differentiation   Flagellum   Kinase   Magnesium   Metal-binding   Nucleotide-binding   Phosphoprotein   Reference proteome   Spermatogenesis   Transferase   Ubl conjugation  

Feature

chain  Testis-specific serine/threonine-protein kinase 4
splice variant  In isoform 2, isoform 3 and isoform 4.

Uniprot

H9J3E1   A0A2H1VJQ2   A0A2A4K5D8   A0A3S2NYC8   A0A194QVR3   A0A194QEE1   + More
A0A212EX65   A0A0F6MU06   E0VRV9   A0A310SJI7   A0A0L7QR24   A0A2A3EMQ4   A0A088AC91   A0A154P123   A0A026X0D2   A0A1Y1L4T1   A0A195EXC5   D6WSN9   A0A151XGT1   A0A195E324   E9IJK0   A0A1Y1L9I8   F4X0Y5   A0A158P1E7   A0A151I0H2   N6UEK6   U4U5E8   A0A1S3CVF1   A0A0J7KQH3   A0A1B6LUN4   A0A1S3D7J2   A0A1B6ERU0   A0A0N0BEA6   K7J3K5   A0A2S2Q637   J9JT13   A0A1B6JW78   T1J6S0   A0A1B0BA31   A0A1A9YDX7   B4P0N4   A0A1A9W2K4   A0A336LIK9   B4KGE2   Q8T4D4   B3N5G4   B4Q4J5   B4I1S5   B4MUV4   A0A0L0CHJ8   A0A1I8N8X0   B4LR54   A0A0K8WAN1   B3MKH2   A0A2R3VH58   A0A034VZM4   A0A0K8W0U9   A0A0K8W4R4   W8CA29   B4JBT0   A0A0K8VCZ5   A0A1W4UXS9   A0A3B0KD15   W8BTY9   C3XTU7   A0A0A1XFU3   A0A0M5IW08   A0A1I8NS29   A0A1S3H1Q0   W4XJ48   A0A232F7V7   T1JN33   N6WBR0   A0A1Q3FI56   B0W2Z6   K1RPQ0   V4A461   A0A023EQI3   A0A2G8LFK9   A0A182G7Q1   A0A210Q8P5   A0A3L5TRX0   A0A182GJF7   A7SRN4   T2M655   A0A3L7GQZ7   Q9D411   A0A3Q7NWT9   F6RLA2   A0A3M6UB54   A0A1A6HWV7   A0A2U3W2C4   A0A2Y9HNY4   A0A2U3Y539   A0A2B4RMH1   A0A212CTI1   A0A1S3FW99   L5M272  

EC Number

2.7.11.1

Pubmed

19121390    26354079    22118469    20566863    24508170    30249741    + More
28004739    18362917    19820115    21282665    21719571    21347285    23537049    20075255    17994087    17550304    10731132    12537568    12537572    12537573    12537574    16110336    17569856    17569867    26109357    26109356    22936249    26108605    25315136    25348373    24495485    18563158    25830018    28648823    15632085    22992520    23254933    24945155    29023486    26483478    28812685    26977809    17615350    24065732    29704459    17927909    20729278    16141072    19468303    23599433    23054012    25361759    26940607    26961893    19393038    30382153   

EMBL

BABH01021512    ODYU01002920    SOQ41021.1    NWSH01000111    PCG79447.1    RSAL01000096    + More
RVE47718.1    KQ461073    KPJ09623.1    KQ459185    KPJ03315.1    AGBW02011827    OWR46075.1    JX173070    AFO67947.1    AAZO01004819    DS235562    EEB16115.1    KQ764369    OAD54532.1    KQ414784    KOC61080.1    KZ288210    PBC32970.1    KQ434796    KZC05533.1    KK107046    QOIP01000004    EZA61755.1    RLU24081.1    GEZM01065018    JAV68594.1    KQ981928    KYN32878.1    KQ971354    EFA07134.2    KQ982138    KYQ59606.1    KQ979709    KYN19481.1    GL763868    EFZ19239.1    GEZM01065017    JAV68595.1    GL888505    EGI59887.1    ADTU01006395    ADTU01006396    ADTU01006397    KQ976633    KYM78875.1    APGK01025210    APGK01025211    APGK01025212    KB740577    ENN80185.1    KB631756    ERL85821.1    LBMM01004365    KMQ92509.1    GEBQ01012583    JAT27394.1    GECZ01029075    JAS40694.1    KQ435834    KOX71604.1    GGMS01003994    MBY73197.1    ABLF02020202    ABLF02020204    GECU01004270    JAT03437.1    JH431885    JXJN01010706    CM000157    EDW87929.1    UFQT01000014    SSX17686.1    CH933807    EDW12138.1    AE014134    AY089244    AAF52349.2    AAL89982.1    CH954177    EDV59043.2    CM000361    CM002910    EDX03922.1    KMY88478.1    CH480820    EDW54482.1    CH963857    EDW76299.1    JRES01000477    KNC30954.1    CH940649    EDW63518.1    GDHF01004409    JAI47905.1    CH902620    EDV31525.1    MF101663    AVQ54901.1    GAKP01011632    GAKP01011631    JAC47320.1    GDHF01007547    JAI44767.1    GDHF01006223    JAI46091.1    GAMC01006131    JAC00425.1    CH916368    EDW03015.1    GDHF01015593    JAI36721.1    OUUW01000010    SPP86130.1    GAMC01006132    JAC00424.1    GG666464    EEN68321.1    GBXI01004486    JAD09806.1    CP012523    ALC38361.1    AAGJ04033422    NNAY01000782    OXU26538.1    AFFK01014481    CH379058    ENO01619.2    GFDL01007843    JAV27202.1    DS231829    EDS30355.1    JH818967    EKC36381.1    KB202544    ESO89780.1    GAPW01002253    JAC11345.1    MRZV01000095    PIK59027.1    JXUM01046754    JXUM01046755    KQ561490    KXJ78402.1    NEDP02004580    OWF45113.1    KV587688    OPL32671.1    JXUM01012826    JXUM01012827    JXUM01012828    DS469765    EDO33625.1    HAAD01001357    CDG67589.1    RAZU01001829    RLQ56116.1    AY461664    EF127819    EF127820    EF127821    EF127822    DQ397204    DQ397205    DQ397206    AK006144    AK016890    AC174678    CH466535    ABD59200.1    ABD59201.1    ABO33082.1    ABO33083.1    ABO33084.1    ABO33085.1    EDL36264.1    EDL36265.1    RCHS01001883    RMX50890.1    LZPO01007973    OBS82943.1    LSMT01000391    PFX18811.1    MKHE01000012    OWK09307.1    KB105273    ELK32461.1   

Proteomes

UP000005204    UP000218220    UP000283053    UP000053240    UP000053268    UP000007151    + More
UP000009046    UP000053825    UP000242457    UP000005203    UP000076502    UP000053097    UP000279307    UP000078541    UP000007266    UP000075809    UP000078492    UP000007755    UP000005205    UP000078540    UP000019118    UP000030742    UP000079169    UP000036403    UP000053105    UP000002358    UP000007819    UP000092460    UP000092443    UP000002282    UP000091820    UP000009192    UP000000803    UP000008711    UP000000304    UP000001292    UP000007798    UP000037069    UP000095301    UP000008792    UP000007801    UP000001070    UP000192221    UP000268350    UP000001554    UP000092553    UP000095300    UP000085678    UP000007110    UP000215335    UP000001819    UP000002320    UP000005408    UP000030746    UP000230750    UP000069940    UP000249989    UP000242188    UP000001593    UP000273346    UP000000589    UP000286641    UP000009136    UP000275408    UP000092124    UP000245340    UP000248481    UP000245341    UP000225706    UP000081671   

PRIDE

H9J3E1    Q8T4D4    Q9D411   

Pfam

PF00069   Pkinase        + More
PF00226   DnaJ

Interpro

IPR000719   Prot_kinase_dom        + More
IPR011009   Kinase-like_dom_sf       
IPR008271   Ser/Thr_kinase_AS       
IPR017441   Protein_kinase_ATP_BS       
IPR001623   DnaJ_domain       
IPR018253   DnaJ_domain_CS       
IPR036869   J_dom_sf       

SUPFAM

SSF56112   SSF56112        + More
SSF46565   SSF46565       

Gene 3D

1.10.287.110    

CDD

cd06257  DnaJ

ProteinModelPortal

H9J3E1   A0A2H1VJQ2   A0A2A4K5D8   A0A3S2NYC8   A0A194QVR3   A0A194QEE1   + More
A0A212EX65   A0A0F6MU06   E0VRV9   A0A310SJI7   A0A0L7QR24   A0A2A3EMQ4   A0A088AC91   A0A154P123   A0A026X0D2   A0A1Y1L4T1   A0A195EXC5   D6WSN9   A0A151XGT1   A0A195E324   E9IJK0   A0A1Y1L9I8   F4X0Y5   A0A158P1E7   A0A151I0H2   N6UEK6   U4U5E8   A0A1S3CVF1   A0A0J7KQH3   A0A1B6LUN4   A0A1S3D7J2   A0A1B6ERU0   A0A0N0BEA6   K7J3K5   A0A2S2Q637   J9JT13   A0A1B6JW78   T1J6S0   A0A1B0BA31   A0A1A9YDX7   B4P0N4   A0A1A9W2K4   A0A336LIK9   B4KGE2   Q8T4D4   B3N5G4   B4Q4J5   B4I1S5   B4MUV4   A0A0L0CHJ8   A0A1I8N8X0   B4LR54   A0A0K8WAN1   B3MKH2   A0A2R3VH58   A0A034VZM4   A0A0K8W0U9   A0A0K8W4R4   W8CA29   B4JBT0   A0A0K8VCZ5   A0A1W4UXS9   A0A3B0KD15   W8BTY9   C3XTU7   A0A0A1XFU3   A0A0M5IW08   A0A1I8NS29   A0A1S3H1Q0   W4XJ48   A0A232F7V7   T1JN33   N6WBR0   A0A1Q3FI56   B0W2Z6   K1RPQ0   V4A461   A0A023EQI3   A0A2G8LFK9   A0A182G7Q1   A0A210Q8P5   A0A3L5TRX0   A0A182GJF7   A7SRN4   T2M655   A0A3L7GQZ7   Q9D411   A0A3Q7NWT9   F6RLA2   A0A3M6UB54   A0A1A6HWV7   A0A2U3W2C4   A0A2Y9HNY4   A0A2U3Y539   A0A2B4RMH1   A0A212CTI1   A0A1S3FW99   L5M272  

PDB

4D28     E-value=1.32499e-49,     Score=495

KEGG

101746605    K08811 testis-specific serine kinase [EC:2.7.11.1] | (RefSeq) testis-specific serine/threonine-prote

GO

GO:0005524   GO:0004674   GO:0004683   GO:0004672   GO:0005737   GO:0006468   GO:0005634   GO:0035556   GO:0016021   GO:0044877   GO:0042803   GO:0009566   GO:0036126   GO:0031514   GO:0001669   GO:0032793   GO:0018105   GO:0097722   GO:1990443   GO:0000287   GO:0007286   GO:0007275   GO:0004713   GO:0004888   GO:0007166   GO:0003707   GO:0006418   GO:0003824  

Subcellular location

Cytoplasmic vesicle   In spermatozoa, present in the sperm head and in the flagellum.   With evidence from 5 publications.
Secretory vesicle   In spermatozoa, present in the sperm head and in the flagellum.   With evidence from 5 publications.
Acrosome   In spermatozoa, present in the sperm head and in the flagellum.   With evidence from 5 publications.
Cell projection   In spermatozoa, present in the sperm head and in the flagellum.   With evidence from 5 publications.
Cilium   In spermatozoa, present in the sperm head and in the flagellum.   With evidence from 5 publications.
Flagellum   In spermatozoa, present in the sperm head and in the flagellum.   With evidence from 5 publications.

Length:

343

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.24118

Exp number, first 60 AAs:

0.00231

Total prob of N-in:

0.01617

outside

1  -  343

 

 

Pi

167.113316

Theta

150.298436

Tajima's D

0.381829

CLR

0.228538

CSRT

0.474326283685816

Interpretation

Uncertain