Gene

KWMTBOMO11484

Pre Gene Modal

BGIBMGA001905

Annotation

PREDICTED:_peptidyl-alpha-hydroxyglycine_alpha-amidating_lyase_2-like_[Papilio_xuthus]

Location

Bomo_Chr19(+):6746017-6748353      View in EpiBrowser

Full name

Peptidyl-alpha-hydroxyglycine alpha-amidating lyase 2      

Alternative Name

Peptidylamidoglycolate lyase 2

Location in the cell

Extracellular   Reliability : 2.077

CDS

ATGTTGTTCGTTTTACTGTACGCCGTAATTCTTAATGGCATCAATTGCGAGCCGGAAGCGGTTAGGGATAACTTTGACTACTTCAACTATGGCGCGAACGATGATGTTTTGAAAAACTTAGAATCTCAATTGTCGAAAGATGAAGTGGTCTTGAGACCTCAAGAGGTCAAAGACTGGCCACAGCAATCTTTGAATGTCGGACAAATAACAGCAGTATCCATTAATTCTTTGGGACAGCCCGTGATATTTCACAGGGCAGATCGAGTATGGGACGAAAATACTTTCAACGAATCCAATGCTTATCAAAACTTCGACAAGGGACCTATAGTTGAAGATACAATTCTTGTTCTTGACCCTGGTAGCGGCTCCGTCCTACATAGCTGGGGAGCTTATATTTTTTATATGCCTCATGGCCTTACCCTCGATCATCATGATAACGTGTGGGTAACTGACGTCGCAAAACATCAAGTATATAAGTATACGCCAAGTAACCACAGATATCCGACCCTGACTATTGGCGAACCCTTCACAGCTGGTCTCCCATTCAGGCATCGTGTTTTATTTTGCATGCCTACATCAGTAGCAATTGCTAGCACCGGAGAAATTTTCGTAGCTGACGGATACTGTAATAATCAAATAGTAAAATTCAATGCCGCTGGGACTCTATTGCTGACGATTCCAGCTTATTCCGACACCTGGTCATTGAATTTGCCTCACAGTGTCACTTTGCTTGAACATTTGGATCTGGTTTGCGTGGCCGATAGAGAAAATATGAGGATCGTGTGCCCGAAGGCTGGATTGAAAAGTTACGCGGATCCGTTAGAGCCACCTACGATAATTGAAGATCCCACTTTGGGCAGGGTGTTTGCAGTGACTTCTCATGGTGATACTATTTACGCTGTGAACGGTCCCACGTCACAGAATATTGCTGTCCGAGGCTTTACTGTTAACGCTGTTTACGGAAACATTTTGGATACTTGGGAACCGACCACTGGATTCACGAACCCACACTCGCTGGCGGTTACAAGGAACGGATCACATCTCTACGTTTCTGAAATTGGACCGAACAAAATCTGGAAGTTTGAATTGACCGACGTATACGATAAGAAATAG

Protein

MLFVLLYAVILNGINCEPEAVRDNFDYFNYGANDDVLKNLESQLSKDEVVLRPQEVKDWPQQSLNVGQITAVSINSLGQPVIFHRADRVWDENTFNESNAYQNFDKGPIVEDTILVLDPGSGSVLHSWGAYIFYMPHGLTLDHHDNVWVTDVAKHQVYKYTPSNHRYPTLTIGEPFTAGLPFRHRVLFCMPTSVAIASTGEIFVADGYCNNQIVKFNAAGTLLLTIPAYSDTWSLNLPHSVTLLEHLDLVCVADRENMRIVCPKAGLKSYADPLEPPTIIEDPTLGRVFAVTSHGDTIYAVNGPTSQNIAVRGFTVNAVYGNILDTWEPTTGFTNPHSLAVTRNGSHLYVSEIGPNKIWKFELTDVYDKK

Description

Probable lyase that catalyzes an essential reaction in C-terminal alpha-amidation of peptides. Mediates the dismutation of the unstable peptidyl(2-hydroxyglycine) intermediate to glyoxylate and the corresponding desglycine peptide amide. C-terminal amidation of peptides such as neuropeptides is essential for full biological activity.

Catalytic Activity

a [peptide]-C-terminal (2S)-2-hydroxyglycine = a [peptide]-C-terminal amide + glyoxylate

Cofactor

Zn(2+)

Biophysicochemical Properties

70 uM for peptidyl-alpha-hydroxyglycine

Similarity

Belongs to the peptidyl-alpha-hydroxyglycine alpha-amidating lyase family.

Keywords

Complete proteome   Disulfide bond   Glycoprotein   Lyase   Metal-binding   Reference proteome   Repeat   Secreted   Signal   Zinc  

Feature

chain  Peptidyl-alpha-hydroxyglycine alpha-amidating lyase 2

Uniprot

H9IXC2   A0A194QW59   A0A194QJL1   A0A212F5R2   A0A2H1WAV2   A0A0L7L3L2   + More
D6WS07   U4UDG4   B4NML5   A0A2J7R7W5   A0A1W4UAC0   A0A182GBJ2   A0A023ERJ6   A0A084WEC6   B3NPQ6   B4P9R3   B4QAX4   B4I8R6   A0A023ESQ7   A0A0B4LG96   Q9W1L5   B4GHU2   A0A182G884   B0WEV2   A0A182NJC7   Q28ZF6   B4KTG3   E0VKF2   A0A1S4G1Z5   A0A023ESU1   B3MFI8   A0A182J0Y5   A0A336N1C7   B4J4G0   A0A336LKU6   A0A034VYR7   A0A3B0J1Z2   A0A2S2QFS7   A0A182MTV0   A0A1Q3FRB9   A0A1Q3FRD4   A0A1Q3FRT6   A0A182FH11   A0A182XMK3   A0A1Q3FRB8   A0A182S982   A0A182TZY5   A0A1Q3FR96   A0A1Q3FRK1   A0A0M4E5T7   Q16GA4   A0A182I2D1   W8AQC3   B4LN29   A0A182WLC9   Q7Q6K9   A0A1B6FIQ8   A0A2M4AY97   A0A182R4J3   A0A182QE76   A0A182PW29   A0A182YEF7   A0A2H8TZW8   A0A1B6I9N2   J9JSH4   A0A0A1WDN3   A0A0A1WE63   W5JAE0   U5ER94   A0A067QWN9   A0A2S2ND71   A0A1B6CDU4   A0A1Y1LW31   A0A1I8MBU3   A0A1B0GBQ4   A0A1I8MBT9   A0A1A9VLB9   A0A1A9YJE4   A0A1B0BZV2   A0A1I8Q792   A0A1A9WQJ0   A0A1A9Z564   A0A0T6BF45   Q171A0   A0A0A9WBR7   N6TA58   A0A026WU18   A0A2P8YNJ4   A0A1W4WYG3   A0A1B6MB47   A0A348G613   A0A1D2N973   A0A226EN09   E2AW67   A0A0P5LX19   A0A0N8AQ75   A0A0P5KUR2   E9HAK8   A0A0P5SHW4   A0A0J7N5A8  

EC Number

4.3.2.5

Pubmed

19121390    26354079    22118469    26227816    18362917    19820115    + More
23537049    17994087    26483478    24945155    24438588    17550304    22936249    10731132    12537568    12537572    12537573    12537574    16110336    17569856    17569867    12537569    15198673    15632085    20566863    25348373    17510324    24495485    12364791    14747013    17210077    25244985    25830018    20920257    23761445    24845553    28004739    25315136    25401762    26823975    24508170    30249741    29403074    27289101    20798317    21292972   

EMBL

BABH01017977    KQ461073    KPJ09712.1    KQ458575    KPJ05717.1    AGBW02010153    + More
OWR49064.1    ODYU01007425    SOQ50188.1    JTDY01003185    KOB70005.1    KQ971351    EFA06405.1    KB632085    ERL88641.1    CH964282    EDW85604.1    NEVH01006723    PNF36923.1    JXUM01052604    KQ561742    KXJ77670.1    GAPW01001778    JAC11820.1    ATLV01023201    KE525341    KFB48570.1    CH954179    EDV56847.1    CM000158    EDW92371.1    CM000362    CM002911    EDX08411.1    KMY96086.1    CH480824    EDW56991.1    GAPW01001777    JAC11821.1    AE013599    AHN56571.1    AY007228    AY119165    CH479183    EDW36062.1    JXUM01008139    KQ560254    KXJ83535.1    DS231911    EDS45865.1    CM000071    EAL25657.1    CH933808    EDW08524.1    DS235243    EEB13867.1    GAPW01001240    JAC12358.1    CH902619    EDV36673.1    UFQS01001876    UFQT01001876    SSX12635.1    SSX32078.1    CH916367    EDW01642.1    UFQS01000040    UFQT01000040    SSW98204.1    SSX18590.1    GAKP01010487    GAKP01010486    JAC48466.1    OUUW01000001    SPP72982.1    GGMS01006839    MBY76042.1    AXCM01019187    GFDL01004875    JAV30170.1    GFDL01004865    JAV30180.1    GFDL01004902    JAV30143.1    GFDL01004854    JAV30191.1    GFDL01004874    JAV30171.1    GFDL01004848    JAV30197.1    CP012524    ALC41716.1    CH478302    EAT33269.1    APCN01000146    GAMC01015575    GAMC01015574    GAMC01015573    GAMC01015572    JAB90980.1    CH940648    EDW60033.1    AAAB01008960    EAA11890.4    GECZ01019681    JAS50088.1    GGFK01012237    MBW45558.1    AXCN02000448    GFXV01008038    MBW19843.1    GECU01024061    JAS83645.1    ABLF02036142    ABLF02036149    GBXI01017290    JAC97001.1    GBXI01017095    JAC97196.1    ADMH02002052    ETN59749.1    GANO01002906    JAB56965.1    KK853156    KDR10480.1    GGMR01002448    MBY15067.1    GEDC01025672    JAS11626.1    GEZM01045591    JAV77732.1    CCAG010005864    JXJN01023308    JXJN01023309    LJIG01001067    KRT85873.1    CH477462    EAT40544.1    GBHO01038390    GDHC01012169    JAG05214.1    JAQ06460.1    APGK01045474    APGK01045475    KB741037    ENN74603.1    KK107105    QOIP01000011    EZA59478.1    RLU16802.1    PYGN01000467    PSN45830.1    GEBQ01006822    JAT33155.1    FX985552    BBF97886.1    LJIJ01000138    ODN01798.1    LNIX01000003    OXA58670.1    GL443249    EFN62324.1    GDIQ01188313    JAK63412.1    GDIQ01253659    JAJ98065.1    GDIQ01186760    JAK64965.1    GL732612    EFX71247.1    GDIP01139550    JAL64164.1    LBMM01009775    KMQ87880.1   

Proteomes

UP000005204    UP000053240    UP000053268    UP000007151    UP000037510    UP000007266    + More
UP000030742    UP000007798    UP000235965    UP000192221    UP000069940    UP000249989    UP000030765    UP000008711    UP000002282    UP000000304    UP000001292    UP000000803    UP000008744    UP000002320    UP000075884    UP000001819    UP000009192    UP000009046    UP000007801    UP000075880    UP000001070    UP000268350    UP000075883    UP000069272    UP000076407    UP000075901    UP000075902    UP000092553    UP000008820    UP000075840    UP000008792    UP000075920    UP000007062    UP000075900    UP000075886    UP000075885    UP000076408    UP000007819    UP000000673    UP000027135    UP000095301    UP000092444    UP000078200    UP000092443    UP000092460    UP000095300    UP000091820    UP000092445    UP000019118    UP000053097    UP000279307    UP000245037    UP000192223    UP000094527    UP000198287    UP000000311    UP000000305    UP000036403   

PRIDE

H9IXC2    B3NPQ6    Q9W1L5   

Pfam

PF01436   NHL

Interpro

IPR001258   NHL_repeat        + More
IPR000720   PHM/PAL       
IPR011042   6-blade_b-propeller_TolB-like       
IPR013017   NHL_repeat_subgr       

Gene 3D

2.120.10.30    

ProteinModelPortal

H9IXC2   A0A194QW59   A0A194QJL1   A0A212F5R2   A0A2H1WAV2   A0A0L7L3L2   + More
D6WS07   U4UDG4   B4NML5   A0A2J7R7W5   A0A1W4UAC0   A0A182GBJ2   A0A023ERJ6   A0A084WEC6   B3NPQ6   B4P9R3   B4QAX4   B4I8R6   A0A023ESQ7   A0A0B4LG96   Q9W1L5   B4GHU2   A0A182G884   B0WEV2   A0A182NJC7   Q28ZF6   B4KTG3   E0VKF2   A0A1S4G1Z5   A0A023ESU1   B3MFI8   A0A182J0Y5   A0A336N1C7   B4J4G0   A0A336LKU6   A0A034VYR7   A0A3B0J1Z2   A0A2S2QFS7   A0A182MTV0   A0A1Q3FRB9   A0A1Q3FRD4   A0A1Q3FRT6   A0A182FH11   A0A182XMK3   A0A1Q3FRB8   A0A182S982   A0A182TZY5   A0A1Q3FR96   A0A1Q3FRK1   A0A0M4E5T7   Q16GA4   A0A182I2D1   W8AQC3   B4LN29   A0A182WLC9   Q7Q6K9   A0A1B6FIQ8   A0A2M4AY97   A0A182R4J3   A0A182QE76   A0A182PW29   A0A182YEF7   A0A2H8TZW8   A0A1B6I9N2   J9JSH4   A0A0A1WDN3   A0A0A1WE63   W5JAE0   U5ER94   A0A067QWN9   A0A2S2ND71   A0A1B6CDU4   A0A1Y1LW31   A0A1I8MBU3   A0A1B0GBQ4   A0A1I8MBT9   A0A1A9VLB9   A0A1A9YJE4   A0A1B0BZV2   A0A1I8Q792   A0A1A9WQJ0   A0A1A9Z564   A0A0T6BF45   Q171A0   A0A0A9WBR7   N6TA58   A0A026WU18   A0A2P8YNJ4   A0A1W4WYG3   A0A1B6MB47   A0A348G613   A0A1D2N973   A0A226EN09   E2AW67   A0A0P5LX19   A0A0N8AQ75   A0A0P5KUR2   E9HAK8   A0A0P5SHW4   A0A0J7N5A8  

PDB

5WM0     E-value=6.27381e-61,     Score=593

KEGG

101742958    K18200 peptidylamidoglycolate lyase [EC:4.3.2.5] | (RefSeq) peptidyl-alpha-hydroxyglycine alpha-amid

GO

GO:0006518   GO:0003824   GO:0016020   GO:0016829   GO:0004497   GO:0004598   GO:0001519   GO:0005576   GO:0004504   GO:0016491   GO:0044237   GO:0046872   GO:0016021   GO:0005515   GO:0042765   GO:0016010   GO:0016740   GO:0003707   GO:0006418  

Subcellular location

Secreted  

SignalP

Position:   1 - 16,         Likelihood:  0.979757

 

 

Length:

370

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.08981

Exp number, first 60 AAs:

0.01025

Total prob of N-in:

0.00252

outside

1  -  370

 

 

Pi

210.958163

Theta

180.002528

Tajima's D

0.998119

CLR

0.00904

CSRT

0.659567021648918

Interpretation

Uncertain