Gene

KWMTBOMO11320

Pre Gene Modal

BGIBMGA001982

Annotation

PREDICTED:_peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine_amidase_[Bombyx_mori]

Location

Bomo_Chr19(-):3171422-3181290      View in EpiBrowser

Full name

Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase      

Alternative Name

Peptide:N-glycanase
N-glycanase 1

Location in the cell

Nuclear   Reliability : 2.028

CDS

ATGGAGGACACAGTGCATTTAGCTTTAGTCGAGCAGAGTATTCGGAACACAGATAAATTCATTATAGCTCTCTACGAACTCTTGGAACGCATCACTCGTATTCTGGAGAATCCGCATGATTACGAATTGAGGAGTATTAAAAAAAATGTTTTCAAAGATTTATCGAAACTGGACTCTTTTAATGAATACATGAAGTATATCGGATTCAAATCGGTAGACAACGAATTTACATATCCAAAGGAGTTAAGTTTTAGCAAATTAAGGATGGCTCAAGTGGCCATCGAAAGGAAACTACATTTTTGCTGTGGATCCGTGCCTATTAGGCCGATCCCAGTAAATTCTACAGACGTTAGAGAACAACCTAAAGCATCCCCAGTTCATTCATTGCAAACTAAAAACAGATTCCTACTCAAAATTCAAGATCTATTCAATGGTATGCAAGTTTATGAGGATGAAGATTTATTAGCACATGCCCGAGACCAAATACCCTTAGTGACATTGCAGCTTATGGCTTTAGACCGAGTTAGGGAACAACAGAAGAAAATTAAGATGGGGGAAATAAAAGCAAACGATCTACCGTTTGATACAGCTTTGCTAATGGAGCTCCTGGACTGGTTCAAGCATGATTTCTTTAAATGGGTCGACAAGCCTGACTGTGATTTATGCGGAGAACGAACCGTCAATCATGAAAACGCTATCATGACGATAGAAGGTGAAACTTGTAGAGTTGAGCTCTACCAGTGCACCGTATGTGAAGGAGGAACCGCGATGTTCCCGCGCTACAATAACCTGCGTACATTGCTGCGCACGAGGAGTGGTCGGTGCGGCGAATGGGCCAATTGCTTTACGCTGCTGTGTCGTGCTTTGGGCTACGACACCAGATACGTTTATGACGTCACCGATCACGTGTGGTGCGAGGTGTTCGACTACGACTCGAACCAGTGGCTGCACGTGGACCCGTGCGAGGCGAAGCTGAACACGCCCCTCATGTACTCGCACGGCTGGGGCAAACGCCTCTCTTACGTCATCGCCTTCTCGAGGGACGACCTGCAGGACGTGACGTGGCGGTACACCACCAACCATAAAGAGGTGTTGAAATACCGCTACCTGTGCACGGAAACAGAATTGATCTCCACGATAATGACGCTGCGGAAGCACAAACAGCGCGATGTAACGGAGGCCAGGCGACGCTATCTAGCCAAGCGCACTCTCGAGGAACTAGTGCAGATGATGGTGGAAAGGAAACCGAGCGACTACGAGTCCCACGGGCGTATATCCGGGTCGCGCAAGTGGCGGATGCAGCGCGGGGAGCTGGGCGGTGCGGCGGGCGGGGCGGTGGGGGGGCACACGTTCGTGCTGGGGCGCGCGGGCACGCACGTGATGCGCTACTACTGCGGCGCCGACCGGTGGAGGCGGGCGTGCGACGGCGCCGAGACCGCGCCCCTGCCCGGCTGGCACGCGGCCGTGCTGCACGCCGCCAACGTGTTCAGGAAAGTCGAGCCGGACTGGCTGCAGTCCTACATAGCTAGAGAAGAGGGCGAGGACTTCGGTTCGATCTCGTGGGCGTTCGCGGCTAGTGAGGAGCTGACGTGCGCCAGTTTATCGATAAAGGTCCGCACGGCGCTGTACGAGAGCGGCCGCATCGACTGGACGGTGAAGTTCGACGATGAAAACCCTACCACGGTCACGTTGAGCGATAAACCAACGAAGTTCGCACGAAAGTTTCGGAAGGTCATCATCAAAGCCGAGCTGTCGGGCGGCGACGGCCCCGTGCGCTGGCAACACGCCCAGCTGTTCCGGCAACACACGTACTCCAAGAGAAGCTCCTTTATCGTCACAATCGTGGTCCAGTAA

Protein

MEDTVHLALVEQSIRNTDKFIIALYELLERITRILENPHDYELRSIKKNVFKDLSKLDSFNEYMKYIGFKSVDNEFTYPKELSFSKLRMAQVAIERKLHFCCGSVPIRPIPVNSTDVREQPKASPVHSLQTKNRFLLKIQDLFNGMQVYEDEDLLAHARDQIPLVTLQLMALDRVREQQKKIKMGEIKANDLPFDTALLMELLDWFKHDFFKWVDKPDCDLCGERTVNHENAIMTIEGETCRVELYQCTVCEGGTAMFPRYNNLRTLLRTRSGRCGEWANCFTLLCRALGYDTRYVYDVTDHVWCEVFDYDSNQWLHVDPCEAKLNTPLMYSHGWGKRLSYVIAFSRDDLQDVTWRYTTNHKEVLKYRYLCTETELISTIMTLRKHKQRDVTEARRRYLAKRTLEELVQMMVERKPSDYESHGRISGSRKWRMQRGELGGAAGGAVGGHTFVLGRAGTHVMRYYCGADRWRRACDGAETAPLPGWHAAVLHAANVFRKVEPDWLQSYIAREEGEDFGSISWAFAASEELTCASLSIKVRTALYESGRIDWTVKFDDENPTTVTLSDKPTKFARKFRKVIIKAELSGGDGPVRWQHAQLFRQHTYSKRSSFIVTIVVQ

Description

Specifically deglycosylates the denatured form of N-linked glycoproteins in the cytoplasm and assists their proteasome-mediated degradation. Cleaves the beta-aspartyl-glucosamine (GlcNAc) of the glycan and the amide side chain of Asn, converting Asn to Asp. Prefers proteins containing high-mannose over those bearing complex type oligosaccharides. Can recognize misfolded proteins in the endoplasmic reticulum that are exported to the cytosol to be destroyed and deglycosylate them, while it has no activity toward native proteins. Deglycosylation is a prerequisite for subsequent proteasome-mediated degradation of some, but not all, misfolded glycoproteins (By similarity).

Catalytic Activity

Hydrolysis of an N(4)-(acetyl-beta-D-glucosaminyl)asparagine residue in which the glucosamine residue may be further glycosylated, to yield a (substituted) N-acetyl-beta-D-glucosaminylamine and a peptide containing an aspartate residue.

Cofactor

Zn(2+)

Similarity

Belongs to the serpin family.
Belongs to the transglutaminase-like superfamily. PNGase family.

Keywords

Complete proteome   Cytoplasm   Hydrolase   Metal-binding   Phosphoprotein   Reference proteome   Zinc  

Feature

chain  Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase

Uniprot

H9IXJ9   A0A2A4JM22   A0A212F0C5   A0A2H1W1F1   A0A2W1BNP0   A0A1E1WPZ4   + More
A0A0L7L9N5   A0A194QX43   A0A194QEW6   A0A1Y1M5T0   A0A1Y1M1G6   A0A139WEQ5   A0A1W4WH01   A0A067QFL0   A0A1Q3FIL6   B0WT33   A0A1L8DU46   V5GPK1   B4MYN5   B4KSC7   A0A2J7R3K4   A0A0M4E457   A0A1S4G2F7   A0A2J7R3J7   Q16G66   B4MDY1   A0A2J7R3M4   Q7KRR5   Q28YQ7   A0A1I8Q858   A0A1J1INN8   B3N3G8   B4GIZ8   B4II25   B4QCQ6   A0A093GVI2   A0A3B0JXM6   A0A182GLV7   B4J9V1   B4NYW0   A0A1B0CRQ6   W8APL2   A0A3P9PDU0   A0A087XNK1   A0A3B3VUN1   G3WDQ9   A0A3B3WEZ1   A0A3Q2PTR9   U5EUG5   G3WDQ8   A0A3P9PDR4   H9GEH0   M4AR69   A0A3B3WEH7   H2SWE9   A0A1W4V2C7   A0A1A9YE51   F4WHB9   F7D099   A0A3P9KYM2   A0A034V3S8   H2LXI6   A0A0K8VS08   A0A315VXC2   T1IJ72   A0A1A8ITM1   A0A1A9V9X0   A0A2R5LEY2   A0A0L7R362   A0A0K8TMU0   A0A146ZNH6   A0A1S3JJE2   A0A3N0YI91   A0A1W4UNC6   A0A1I8MV56   A0A2K6EN32   A0A1S3K0I9   A0A158NB52   A0A3P9H9I3   G3NZ69   A0A146V9E4   A0A093QPG4   H0WKM6   A0A369SB05   A0A195D6P1   B3MJ81   B3RRV6   A0A1Z5KUR9   A0A099YVZ1   A0A0L0CPF8   A0A0F8C8M8   A0A3Q3VZD1   A0A224Z3V3   W5UQD7   A7SSF4   A0A131XD48   A0A091FNN0   Q5ZJM3   F7AI61   A0A093KCM9  

EC Number

3.5.1.52

Pubmed

19121390    22118469    28756777    26227816    26354079    28004739    + More
18362917    19820115    24845553    17994087    17510324    18057021    10831608    10731132    12537572    12537569    18327897    15632085    22936249    26483478    17550304    24495485    21709235    21881562    23542700    21551351    21719571    17495919    17554307    25348373    29703783    26369729    26383154    25315136    21347285    30042472    18719581    28528879    26108605    25835551    28797301    23127152    17615350    28049606    15642098   

EMBL

BABH01018274    BABH01018275    NWSH01001016    PCG73107.1    AGBW02011140    OWR47151.1    + More
ODYU01005752    SOQ46925.1    KZ150054    PZC74350.1    GDQN01002002    JAT89052.1    JTDY01002074    KOB72172.1    KQ461073    KPJ09530.1    KQ459053    KPJ04083.1    GEZM01043172    JAV79256.1    GEZM01043176    JAV79251.1    KQ971354    KYB26414.1    KK853579    KDR06555.1    GFDL01007615    JAV27430.1    DS232080    EDS34176.1    GFDF01004121    JAV09963.1    GALX01002422    JAB66044.1    CH963894    EDW77224.1    CH933808    EDW08409.1    NEVH01007819    PNF35419.1    CP012524    ALC40741.1    PNF35421.1    CH478322    EAT33229.1    CH940662    EDW58746.1    KRF78452.1    PNF35420.1    AF250926    AE013599    AY119239    BT001557    CM000071    CVRI01000054    CRL00097.1    CH954177    EDV59850.1    CH479183    EDW36416.1    CH480841    EDW49551.1    CM000362    CM002911    EDX05845.1    KMY91682.1    KL216727    KFV71082.1    OUUW01000001    SPP75838.1    JXUM01073164    JXUM01073165    KQ562759    KXJ75179.1    CH916367    EDW01515.1    CM000157    EDW89811.1    AJWK01025119    AJWK01025120    GAMC01018588    JAB87967.1    AYCK01007384    AEFK01202071    AEFK01202072    AEFK01202073    AEFK01202074    GANO01001483    JAB58388.1    AAWZ02026560    GL888158    EGI66396.1    GAKP01022529    GAKP01022527    JAC36425.1    GDHF01010652    JAI41662.1    NHOQ01001491    PWA24111.1    JH430232    HAED01014246    SBR00691.1    GGLE01003882    MBY08008.1    KQ414663    KOC65312.1    GDAI01001944    JAI15659.1    GCES01018575    JAR67748.1    RJVU01042534    ROL45873.1    ADTU01010884    GCES01072341    JAR13982.1    KL426509    KFW90446.1    AAQR03005117    AAQR03005118    AAQR03005119    AAQR03005120    AAQR03005121    AAQR03005122    NOWV01000034    RDD43711.1    KQ976760    KYN08531.1    CH902619    EDV38175.1    DS985243    EDV26937.1    GFJQ02008127    JAV98842.1    KL886993    KGL74389.1    JRES01000204    KNC33319.1    KQ041387    KKF27325.1    GFPF01010455    MAA21601.1    JT415651    AHH41778.1    DS469776    EDO33371.1    GEFH01005080    JAP63501.1    KL447195    KFO70734.1    AJ720411    KL206987    KFV88029.1   

Proteomes

UP000005204    UP000218220    UP000007151    UP000037510    UP000053240    UP000053268    + More
UP000007266    UP000192223    UP000027135    UP000002320    UP000007798    UP000009192    UP000235965    UP000092553    UP000008820    UP000008792    UP000000803    UP000001819    UP000095300    UP000183832    UP000008711    UP000008744    UP000001292    UP000000304    UP000053875    UP000268350    UP000069940    UP000249989    UP000001070    UP000002282    UP000092461    UP000242638    UP000028760    UP000261500    UP000007648    UP000261480    UP000265000    UP000001646    UP000002852    UP000005226    UP000192221    UP000092443    UP000007755    UP000002280    UP000265180    UP000001038    UP000078200    UP000053825    UP000085678    UP000095301    UP000233160    UP000005205    UP000265200    UP000007635    UP000005225    UP000253843    UP000078542    UP000007801    UP000009022    UP000053641    UP000037069    UP000261620    UP000221080    UP000001593    UP000053760    UP000000539    UP000008225    UP000053584   

PRIDE

H9IXJ9    Q7KRR5   

Pfam

PF04721   PAW        + More
PF01841   Transglut_core
PF09409   PUB
PF00079   Serpin
PF03835   Rad4

Interpro

IPR002931   Transglutaminase-like        + More
IPR008979   Galactose-bd-like_sf       
IPR038680   PAW_sf       
IPR038765   Papain-like_cys_pep_sf       
IPR006588   Peptide_N_glycanase_PAW_dom       
IPR036339   PUB-like_dom_sf       
IPR018997   PUB_domain       
IPR036186   Serpin_sf       
IPR042178   Serpin_sf_1       
IPR000215   Serpin_fam       
IPR023796   Serpin_dom       
IPR023795   Serpin_CS       
IPR042185   Serpin_sf_2       
IPR018325   Rad4/PNGase_transGLS-fold       

SUPFAM

SSF54001   SSF54001        + More
SSF49785   SSF49785       
SSF143503   SSF143503       
SSF56574   SSF56574       

Gene 3D

2.60.120.1020     2.30.39.10     3.30.497.10    

ProteinModelPortal

H9IXJ9   A0A2A4JM22   A0A212F0C5   A0A2H1W1F1   A0A2W1BNP0   A0A1E1WPZ4   + More
A0A0L7L9N5   A0A194QX43   A0A194QEW6   A0A1Y1M5T0   A0A1Y1M1G6   A0A139WEQ5   A0A1W4WH01   A0A067QFL0   A0A1Q3FIL6   B0WT33   A0A1L8DU46   V5GPK1   B4MYN5   B4KSC7   A0A2J7R3K4   A0A0M4E457   A0A1S4G2F7   A0A2J7R3J7   Q16G66   B4MDY1   A0A2J7R3M4   Q7KRR5   Q28YQ7   A0A1I8Q858   A0A1J1INN8   B3N3G8   B4GIZ8   B4II25   B4QCQ6   A0A093GVI2   A0A3B0JXM6   A0A182GLV7   B4J9V1   B4NYW0   A0A1B0CRQ6   W8APL2   A0A3P9PDU0   A0A087XNK1   A0A3B3VUN1   G3WDQ9   A0A3B3WEZ1   A0A3Q2PTR9   U5EUG5   G3WDQ8   A0A3P9PDR4   H9GEH0   M4AR69   A0A3B3WEH7   H2SWE9   A0A1W4V2C7   A0A1A9YE51   F4WHB9   F7D099   A0A3P9KYM2   A0A034V3S8   H2LXI6   A0A0K8VS08   A0A315VXC2   T1IJ72   A0A1A8ITM1   A0A1A9V9X0   A0A2R5LEY2   A0A0L7R362   A0A0K8TMU0   A0A146ZNH6   A0A1S3JJE2   A0A3N0YI91   A0A1W4UNC6   A0A1I8MV56   A0A2K6EN32   A0A1S3K0I9   A0A158NB52   A0A3P9H9I3   G3NZ69   A0A146V9E4   A0A093QPG4   H0WKM6   A0A369SB05   A0A195D6P1   B3MJ81   B3RRV6   A0A1Z5KUR9   A0A099YVZ1   A0A0L0CPF8   A0A0F8C8M8   A0A3Q3VZD1   A0A224Z3V3   W5UQD7   A7SSF4   A0A131XD48   A0A091FNN0   Q5ZJM3   F7AI61   A0A093KCM9  

PDB

2F4O     E-value=1.58346e-51,     Score=514

KEGG

101738189    K01456 peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase [EC:3.5.1.52] | (RefSeq) peptide-N(

PATHWAY

04141    Protein processing in endoplasmic reticulum - Bombyx mori (domestic silkworm)

GO

GO:0006516   GO:0005737   GO:0005615   GO:0006517   GO:0006515   GO:0000224   GO:0005634   GO:0005829   GO:0007495   GO:0030513   GO:0030246   GO:0046872   GO:0008270   GO:0005515   GO:0042555   GO:0006260   GO:0006811   GO:0005622   GO:0006810   GO:0003824  

PANTHER

PTHR11461  

Subcellular location

Cytoplasm  

Length:

617

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.13835

Exp number, first 60 AAs:

0.00015

Total prob of N-in:

0.00048

outside

1  -  617

 

 

Pi

216.875494

Theta

156.580092

Tajima's D

1.774806

CLR

0.350544

CSRT

0.848307584620769

Interpretation

Uncertain