Gene

KWMTBOMO11276  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA002763

Annotation

PREDICTED:_DNA_replication_licensing_factor_MCM4_[Amyelois_transitella]

Location

Bomo_Chr19(+):2354756-2367788      View in EpiBrowser

Full name

DNA helicase       + More
DNA replication licensing factor MCM4      

Alternative Name

Protein disc proliferation abnormal

Location in the cell

Mitochondrial   Reliability : 2.158

CDS

ATGCCGGCGGGGCGCACCCCGGCCACGGTGAGCGTGGTGGCGCACGGCGCGCTGGTGGAGGCGGCGGGCGCGGGGCGGCGCGTGCGCGTGACGGGCGTGTTCCGCGCCGCGCCCGCCGCGCTGCACCCGCGCCGCGCCACGCTGCGCGCGCTGCACCGCACGCACCTCGACGCGCTGCACTACCGCACCGAGCACGAGAGCAGGCTGCACGACATCGACGACGGCAAAGAGCATCGCTTCCCACCTGAACGCGTCGAGTTATTCAAAACTCTGGCGTCGCAGCCGGATTGCTATGAACGTCTGGCTCGGGCCATTGCGCCTTCGGTGTATGAAAATTTGGATATCAAAAAAGGAGTGCTACTGCAGTTACTCGGAGGGACCAAAAAGAACTTTAATGCCGCTGGTCGGACACATTTTAGAGCTGAAATCAACATATTGCTATGCGGAGACCCTGGTACGTCCAAGTCTCAACTGCTTCGCTGGGTGTACACGTTGGTGCCGAGAGCTCAATACACGTCTGGCCGAGGCTCCTCTGCGGTCGGACTCACTGCTTACGTCACCAAGGACCCTGATACCAGGCAACTTGTATTGCAAACCGGTGCTTTGGTTCTAGCAGACAACGGTATATGCTGTATTGATGAGTTTGACAAAATGAACGATTCCACACGGAGCGTCCTCCACGAGGTGATGGAGCAACAGACACTGTCAATAGCCAAAGCCGGTATCATCTGTCAACTGAACGCTCGCACCTCGATCCTGGCGGCCGCCAACCCCGCCGAGTCGCAGTGGAACAAGAACAAAACGATCGTTGAAAATGTTCAACTACCGCACACGCTCATGTCCAGGTTCGACTTAATATTCCTCGTGCTGGATCCACAAGACGAAGTGTTCGACAGACGTCTGGCCTCGCACCTGGTGTCCCTGTACTACAAAGACCCGAACGATCCTCAAGATGACGAGGATGCTATTGATATAAGTCTGATGCGGGACTACATAGCCTTCGCGAAGGAGCACGTGCAGCCCACGCTCAGCGAGACGGCGCAGCAGCGGCTCATAGACGCCTACGTCGACATGAGGCGTGTGGGCAGCGGCCGAGGTCAGATATCGGCGTATCCGAGGCAGCTCGAATCTCTCATCAGGTTGGCGGAAGCGCACGCCAGGGTTCGACTTTCTTCCGTTGTTGAACTGATAGACGTAGACGAAGCGGCGAGACTTCACCGCGAGGCACTGAAGCAGTCGGCCACGGACCCGGCCTCCGGGCGCATCGACGTGGGCATCCTGACGTGCGGGCTGGGCGCCGCGGCCCGCAAGCGCCGGGCGGACCTGCGCGCCGCGCTCTCCGCTCTCATCCAGCCGCTGCACAAGCCGCTCACCCTCACCCACGCCAAGCTCCTGCACGACATCAACGCCGCCTCGCAAATAACTGTAACAAGAGAGCAGTTAGACGAAGCACTGAGGGACCTACAAGACGAAGGGAAAGTTGTAGTCGTAAGCCATACTCACATCAGATTATGTTGA

Protein

MPAGRTPATVSVVAHGALVEAAGAGRRVRVTGVFRAAPAALHPRRATLRALHRTHLDALHYRTEHESRLHDIDDGKEHRFPPERVELFKTLASQPDCYERLARAIAPSVYENLDIKKGVLLQLLGGTKKNFNAAGRTHFRAEINILLCGDPGTSKSQLLRWVYTLVPRAQYTSGRGSSAVGLTAYVTKDPDTRQLVLQTGALVLADNGICCIDEFDKMNDSTRSVLHEVMEQQTLSIAKAGIICQLNARTSILAAANPAESQWNKNKTIVENVQLPHTLMSRFDLIFLVLDPQDEVFDRRLASHLVSLYYKDPNDPQDDEDAIDISLMRDYIAFAKEHVQPTLSETAQQRLIDAYVDMRRVGSGRGQISAYPRQLESLIRLAEAHARVRLSSVVELIDVDEAARLHREALKQSATDPASGRIDVGILTCGLGAAARKRRADLRAALSALIQPLHKPLTLTHAKLLHDINAASQITVTREQLDEALRDLQDEGKVVVVSHTHIRLC

Description

Acts as component of the Mcm2-7 complex (Mcm complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the Mcm2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation. Essential role in mitotic DNA replication but not in endoreplication.

Catalytic Activity

ATP + H2O = ADP + H(+) + phosphate

Subunit

Component of the Mcm2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order Mcm2-Mcm6-Mcm4-Mcm7-Mcm3-Mcm5 (Probable).

Similarity

Belongs to the MCM family.

Keywords

ATP-binding   Complete proteome   DNA replication   DNA-binding   Helicase   Hydrolase   Nucleotide-binding   Nucleus   Phosphoprotein   Reference proteome  

Feature

chain  DNA replication licensing factor MCM4

Uniprot

S4P9E2   A0A2A4K0L3   A0A2H1WYC1   A0A194QF49   A0A212FER1   D6WSI2   + More
A0A1B6LKZ4   A0A182ILA0   A0A084WE11   B3N9Y3   B4P1Q4   N6THZ9   A0A182N1Y6   A0A0J9TVC7   B4HQV7   A0A1B0CFB1   A0A0B4LEV2   Q26454   F4X340   A0A195AZD9   A0A336K914   A0A1W4VDK0   E2A9C4   A0A151WQE4   A0A182Q843   A0A158NYJ2   A0A195F6A0   A0A195CWG0   A0A151IXH1   A0A1A9USP5   B0WVG5   A0A182XEH1   A0A182KNP9   A0A182PTV7   A0A182HQL4   A0A182VI32   A0A1Q3EVE7   A0A182JVZ5   A0A182TDY8   Q7PPI5   A0A182M1N7   A0A1W4XPI5   T1EAS0   B4H8K8   A0A182YR51   A0A2J7PJ85   Q28YI1   B4KQ97   A0A182T370   A0A3B0J6N2   A0A182WM22   E2C0X5   A0A3R7PR63   A0A026WMZ8   T1P9F1   V5KX69   A0A182RE53   A0A0M3QUM2   A0A1I8N9V2   B3MGV3   B4MIN6   Q16G49   E9IUU4   A0A1I8QAN5   B4LLC4   A0A0L0C837   A0A0J7L4H7   B4J9Z6   Q16TZ0   A0A067R0Z3   A0A1B6CYY5   W5JWV7   A0A1J1HY95   A0A034W864   A0A0K8WM27   A0A0A1XAF5   A0A232EYL2   A0A182FLN8   A0A131XFL7   K7INC3   W8C9Q3   A0A131YVH2   A0A2L2XZ57   L7M3G2   A0A2P8XCP1   A0A224YV10   A0A1Y1KFA9   A0A293M9W0   E0VGB6   A0A1Y1KIS5   A0A2P2I4F0   A0A293MRR8   A0A182H304   A0A0K2SW79   V5GWV7   A0A0C9QKK7   A0A1E1X8P0   A0A1Z5LEC0   G3MI99   A0A087SWJ6  

EC Number

3.6.4.12

Pubmed

23622113    26354079    22118469    18362917    19820115    24438588    + More
17994087    17550304    23537049    22936249    10731132    12537568    12537572    12537573    12537574    16110336    17569856    17569867    7489728    16798881    17372656    18327897    20122406    21719571    20798317    21347285    20966253    12364791    14747013    17210077    25244985    15632085    24508170    30249741    25315136    17510324    21282665    26108605    24845553    20920257    23761445    25348373    25830018    28648823    28049606    20075255    24495485    26830274    26561354    25576852    29403074    28797301    28004739    20566863    26483478    25765539    28503490    28528879    22216098   

EMBL

GAIX01003789    JAA88771.1    NWSH01000348    PCG77182.1    ODYU01012011    SOQ58075.1    + More
KQ459053    KPJ04044.1    AGBW02008904    OWR52231.1    KQ971352    EFA06648.2    GEBQ01015602    JAT24375.1    ATLV01023101    KE525340    KFB48455.1    CH954177    EDV59679.1    CM000157    EDW89190.1    APGK01025879    KB740605    ENN80029.1    CM002911    KMY92000.1    CH480816    EDW46767.1    AJWK01009886    AE013599    AHN55958.1    S80255    GL888608    EGI59140.1    KQ976701    KYM77324.1    UFQS01000188    UFQT01000188    SSX00981.1    SSX21361.1    GL437781    EFN69966.1    KQ982843    KYQ50028.1    AXCN02000977    ADTU01003928    KQ981768    KYN35911.1    KQ977220    KYN04872.1    KQ980806    KYN12745.1    DS232126    EDS35571.1    APCN01004657    GFDL01015765    JAV19280.1    AAAB01008948    EAA10355.3    AXCM01003001    GAMD01000167    JAB01424.1    CH479223    EDW35043.1    NEVH01024950    PNF16400.1    CM000071    EAL25984.1    CH933808    EDW09225.1    OUUW01000001    SPP75703.1    GL451850    EFN78411.1    QCYY01001161    ROT80053.1    KK107151    QOIP01000003    EZA57318.1    RLU25202.1    KA645406    AFP60035.1    KF471133    AHA42533.1    CP012524    ALC40873.1    CH902619    EDV37871.1    CH963719    EDW71975.1    CH478333    EAT33214.1    GL766050    EFZ15651.1    CH940648    EDW61876.1    JRES01000780    KNC28392.1    LBMM01000833    KMQ97399.1    CH916367    EDW02583.1    CH477637    EAT37976.1    KK852792    KDR16461.1    GEDC01018606    JAS18692.1    ADMH02000108    ETN67810.1    CVRI01000030    CRK92530.1    GAKP01007211    JAC51741.1    GDHF01000205    JAI52109.1    GBXI01006639    JAD07653.1    NNAY01001603    OXU23451.1    GEFH01003616    JAP64965.1    GAMC01001959    JAC04597.1    GEDV01005348    JAP83209.1    IAAA01006367    IAAA01006368    LAA01234.1    GACK01007361    JAA57673.1    PYGN01002995    PSN29765.1    GFPF01007295    MAA18441.1    GEZM01085193    JAV60139.1    GFWV01012142    MAA36871.1    DS235135    EEB12422.1    GEZM01085192    JAV60140.1    IACF01003276    LAB68899.1    GFWV01018806    MAA43534.1    JXUM01024334    JXUM01024335    JXUM01087698    JXUM01087699    KQ563653    KQ560687    KXJ73554.1    KXJ81271.1    HACA01000667    CDW18028.1    GANP01009558    JAB74910.1    GBYB01015313    JAG85080.1    GFAC01003558    JAT95630.1    GFJQ02001265    JAW05705.1    JO841600    AEO33217.1    KK112270    KFM57235.1   

Proteomes

UP000218220    UP000053268    UP000007151    UP000007266    UP000075880    UP000030765    + More
UP000008711    UP000002282    UP000019118    UP000075884    UP000001292    UP000092461    UP000000803    UP000007755    UP000078540    UP000192221    UP000000311    UP000075809    UP000075886    UP000005205    UP000078541    UP000078542    UP000078492    UP000078200    UP000002320    UP000076407    UP000075882    UP000075885    UP000075840    UP000075903    UP000075881    UP000075902    UP000007062    UP000075883    UP000192223    UP000008744    UP000076408    UP000235965    UP000001819    UP000009192    UP000075901    UP000268350    UP000075920    UP000008237    UP000283509    UP000053097    UP000279307    UP000075900    UP000092553    UP000095301    UP000007801    UP000007798    UP000008820    UP000095300    UP000008792    UP000037069    UP000036403    UP000001070    UP000027135    UP000000673    UP000183832    UP000215335    UP000069272    UP000002358    UP000245037    UP000009046    UP000069940    UP000249989    UP000054359   

PRIDE

Q26454   

Pfam

PF00493   MCM        + More
PF17207   MCM_OB
PF17855   MCM_lid
PF14551   MCM_N
PF01448   ELM2

Interpro

IPR041562   MCM_lid        + More
IPR033762   MCM_OB       
IPR027417   P-loop_NTPase       
IPR018525   MCM_CS       
IPR008047   MCM_4       
IPR012340   NA-bd_OB-fold       
IPR031327   MCM       
IPR001208   MCM_dom       
IPR003593   AAA+_ATPase       
IPR027925   MCM_N       
IPR001005   SANT/Myb       
IPR017884   SANT_dom       
IPR009057   Homeobox-like_sf       
IPR000949   ELM2_dom       

SUPFAM

SSF52540   SSF52540        + More
SSF50249   SSF50249       
SSF46689   SSF46689       

ProteinModelPortal

S4P9E2   A0A2A4K0L3   A0A2H1WYC1   A0A194QF49   A0A212FER1   D6WSI2   + More
A0A1B6LKZ4   A0A182ILA0   A0A084WE11   B3N9Y3   B4P1Q4   N6THZ9   A0A182N1Y6   A0A0J9TVC7   B4HQV7   A0A1B0CFB1   A0A0B4LEV2   Q26454   F4X340   A0A195AZD9   A0A336K914   A0A1W4VDK0   E2A9C4   A0A151WQE4   A0A182Q843   A0A158NYJ2   A0A195F6A0   A0A195CWG0   A0A151IXH1   A0A1A9USP5   B0WVG5   A0A182XEH1   A0A182KNP9   A0A182PTV7   A0A182HQL4   A0A182VI32   A0A1Q3EVE7   A0A182JVZ5   A0A182TDY8   Q7PPI5   A0A182M1N7   A0A1W4XPI5   T1EAS0   B4H8K8   A0A182YR51   A0A2J7PJ85   Q28YI1   B4KQ97   A0A182T370   A0A3B0J6N2   A0A182WM22   E2C0X5   A0A3R7PR63   A0A026WMZ8   T1P9F1   V5KX69   A0A182RE53   A0A0M3QUM2   A0A1I8N9V2   B3MGV3   B4MIN6   Q16G49   E9IUU4   A0A1I8QAN5   B4LLC4   A0A0L0C837   A0A0J7L4H7   B4J9Z6   Q16TZ0   A0A067R0Z3   A0A1B6CYY5   W5JWV7   A0A1J1HY95   A0A034W864   A0A0K8WM27   A0A0A1XAF5   A0A232EYL2   A0A182FLN8   A0A131XFL7   K7INC3   W8C9Q3   A0A131YVH2   A0A2L2XZ57   L7M3G2   A0A2P8XCP1   A0A224YV10   A0A1Y1KFA9   A0A293M9W0   E0VGB6   A0A1Y1KIS5   A0A2P2I4F0   A0A293MRR8   A0A182H304   A0A0K2SW79   V5GWV7   A0A0C9QKK7   A0A1E1X8P0   A0A1Z5LEC0   G3MI99   A0A087SWJ6  

PDB

5U8S     E-value=2.16682e-120,     Score=1107

KEGG

101746848    K02212 DNA replication licensing factor MCM4 [EC:3.6.4.12] | (RefSeq) DNA replication licensing fact

PATHWAY

03030    DNA replication - Bombyx mori (domestic silkworm)

GO

GO:0042555   GO:0005524   GO:0003678   GO:0003677   GO:0006270   GO:0005634   GO:0000727   GO:1902975   GO:0003688   GO:0003697   GO:0006267   GO:0006271   GO:0006268   GO:0043138   GO:0007052   GO:0006260   GO:0004386   GO:0006811   GO:0005622   GO:0006810   GO:0003824  

PANTHER

PTHR11630:SF66   PTHR11630  

Subcellular location

Nucleus  

Length:

505

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.06923

Exp number, first 60 AAs:

0.06128

Total prob of N-in:

0.00530

outside

1  -  505

 

 

Pi

191.708944

Theta

160.675191

Tajima's D

0.694578

CLR

120.805828

CSRT

0.571121443927804

Interpretation

Uncertain