Gene

KWMTBOMO11275

Pre Gene Modal

BGIBMGA002762

Annotation

PREDICTED:_DNA_replication_licensing_factor_MCM4_[Amyelois_transitella]

Location

Bomo_Chr19(+):2341796-2354480      View in EpiBrowser

Full name

DNA helicase       + More
DNA replication licensing factor MCM4      
DNA replication licensing factor mcm4-A      
DNA replication licensing factor mcm4      
DNA replication licensing factor mcm4-B      

Alternative Name

Protein disc proliferation abnormal
CDC21 homolog-A
Minichromosome maintenance protein 4-A
P1-CDC21-A
p98
Minichromosome maintenance protein 4
CDC21 homolog-B
Minichromosome maintenance protein 4-B
P1-CDC21-B

Location in the cell

Nuclear   Reliability : 2.169

CDS

ATGTCTACACCATCTAAACGCCCGTCGTCGCGTGCATCATCGGAAGCATCGACTCCACGAAGAACGCGATCCTCACAACAAGTTATCGTTCCGGAGACTCCGCAGCGTTCAACCGGCACACCTTCGAAAGATGGCACTCCAAGTACACCACGAGCAGCTCCCGGTCCATCTCGGACACCCCGTACACCTCGTGAAGCTCCAGGAACATCACCTGCTCGAGCTCTTGCATCCAGTCCCATAGGCACAGATATAGACATGAGCTCTCCTCTTAATTACGGCACTCCAAGTTCTCTAAGCACTCCTCGCTCGCTCATGAGAGGCATCATGACCCCAGCACGACAGAGGGCTGACCTCCGAGGTCACCATATAGCTCCCAATGTACCGGCACCCACACCTACACGGAGAGTGGAGGGTTCAGAGGAGGCTTCGAGTGAGCCGCAGCTGGTCGTGTGGGGGACGGACGTGGCCATCGCAGAATGCAGGGAGAAATTCATCAAGTTCATCCAGAGATACGTGGAGCCAACTGCTGTCACCAACGAACCTCTTTACGAGCAAAAACTTGAGGAGATTCACACATTGGAAGAGCCATTCTTAGATGTGGACTGTGAACATGTCAGAGTTTTCGATTCCAAACTACACAGACAACTGGTCTCTTATCCCCAAGAGGTTGTGCCGGCATTCGACGCAGCAGTCAACGAGCTGTTCTTCGAGAAGTACCCGGCGGCCGTGCTCGAGCACCAGATCCAGGTCCGGCCGTACAACGCCCCGCCGCGACACATGAGAGACCTCAATCCGGAAGACATAGACCAGCTGGTGACTATATCGGGCATGGTGATACGCACGTCCGGCATCGTGCCGGAGATGCGGGAGGCGTTCTTCAAGTGCGCGGTGTGCGGCGCCGCGGCGGGCGGGGAGCTGCAGCGCGGCCGCGTGCCCGAGCCCGCACACTGCGCGCACTGCAACACGGCGCACTGCTTCCAGCTCATACACAACCGCTCGCACTTCAGCGACAAGCAGCTCGTCAAGCTGCAGGAGGCACCTGGTGACTGGTTTTTTTATTTTATTTTTATTGCTTAG

Protein

MSTPSKRPSSRASSEASTPRRTRSSQQVIVPETPQRSTGTPSKDGTPSTPRAAPGPSRTPRTPREAPGTSPARALASSPIGTDIDMSSPLNYGTPSSLSTPRSLMRGIMTPARQRADLRGHHIAPNVPAPTPTRRVEGSEEASSEPQLVVWGTDVAIAECREKFIKFIQRYVEPTAVTNEPLYEQKLEEIHTLEEPFLDVDCEHVRVFDSKLHRQLVSYPQEVVPAFDAAVNELFFEKYPAAVLEHQIQVRPYNAPPRHMRDLNPEDIDQLVTISGMVIRTSGIVPEMREAFFKCAVCGAAAGGELQRGRVPEPAHCAHCNTAHCFQLIHNRSHFSDKQLVKLQEAPGDWFFYFIFIA

Description

Acts as component of the Mcm2-7 complex (Mcm complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the Mcm2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation. Essential role in mitotic DNA replication but not in endoreplication.
Acts as component of the mcm2-7 complex (mcm complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the mcm2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.

Catalytic Activity

ATP + H2O = ADP + H(+) + phosphate

Subunit

Component of the Mcm2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order Mcm2-Mcm6-Mcm4-Mcm7-Mcm3-Mcm5 (Probable).
Component of the mcm2-7 complex (RLF-M). The complex forms a toroidal hexameric ring with the proposed subunit order mcm2-mcm6-mcm4-mcm7-mcm3-mcm5 (Probable). The heterodimer of mmcm3/mcm5 interacts with mcm4, mmcm6, mcm7 and weakly with mcm2.
Component of the mcm2-7 complex (RLF-M). The complex forms a toroidal hexameric ring with the proposed subunit order mcm2-mcm6-mcm4-mcm7-mcm3-mcm5 (Probable). The heterodimer of mmcm3/mcm5 interacts with mcm4, mmcm6, mcm7 and weakly with mcm2. Begins to associate with zmcm6 at the neurula stage (By similarity).
Component of the mcm2-7 complex (RLF-M). The complex forms a toroidal hexameric ring with the proposed subunit order mcm2-mcm6-mcm4-mcm7-mcm3-mcm5 (Probable). The heterodimer of mmcm3/mcm5 interacts with mcm4, mmcm6, mcm7 and weakly with mcm2. Begins to associate with zmcm6 at the neurula stage.

Similarity

Belongs to the MCM family.

Keywords

ATP-binding   Complete proteome   DNA replication   DNA-binding   Helicase   Hydrolase   Nucleotide-binding   Nucleus   Phosphoprotein   Reference proteome   Cell cycle   Metal-binding   Zinc   Zinc-finger  

Feature

chain  DNA replication licensing factor MCM4

Uniprot

A0A212FER1   A0A2H1WYC1   A0A2A4K0L3   A0A194QX82   A0A194QF49   A0A182H304   + More
Q16TZ0   Q5BI95   A0A1Q3EVE7   A0A0K8WM27   A0A034W864   B0WVG5   A0A1I8QAN5   A0A1I8N9V2   A0A1A9USP5   A0A0L0C837   B3N9Y3   B4P1Q4   B3MGV3   A0A0A1XAF5   A0A182VI32   A0A182TDY8   W8C9Q3   A0A0J9TVC7   B4HQV7   Q7PPI5   A0A182XEH1   A0A182KNP9   A0A182HQL4   A0A182ILA0   A0A0B4LEV2   Q26454   B4KQ97   A0A1W4VDK0   A0A182N1Y6   A0A182T370   B4LLC4   A0A0C9QKK7   A0A182WM22   A0A1W4XPI5   A0A182RE53   V5KX69   A0A182PTV7   K7INC3   A0A0M3QUM2   A0A182M1N7   Q28YI1   A0A2P8XCP1   A0A067R0Z3   B4MIN6   A0A084WE11   A0A1B0G5U6   B4K2W4   W5JWV7   A0A3B0J6N2   A0A182JVZ5   A0A1B6KRG0   A0A1B6F0W3   A0A1Y1KIS5   T1P9F1   B4H8K8   A0A182YR51   B4J9Z6   A0A182FLN8   A0A232EYL2   D6WSI2   A0A182Q843   N6THZ9   A0A0J7L4H7   A0A2J7PJ85   A0A026WMZ8   A0A336K914   A0A3R7PR63   A0A1J1HY95   A0A2I4CZX7   A0A3Q2ZSX3   A0A1W5A084   A0A3B3RBL6   A0A1S3LXE0   Q5XK83   A0A1L8FSY2   A0A3Q1HY34   A0A060W8M7   Q6GL41   E2A9C4   E2C0X5   V4A2X0   A0A2P4SU88   A0A146YY99   C0HAN5   A0A3Q2E9N7   A0A2U9BJR0   V8P321   A0A2D4MKN7   A0A3P9AIQ8   A0A3B4V4A7   A0A1L8FYX1   P30664   A0A091R783  

EC Number

3.6.4.12

Pubmed

22118469    26354079    26483478    17510324    25348373    25315136    + More
26108605    17994087    17550304    25830018    24495485    22936249    12364791    14747013    17210077    20966253    10731132    12537568    12537572    12537573    12537574    16110336    17569856    17569867    7489728    16798881    17372656    18327897    20122406    20075255    15632085    29403074    24845553    24438588    20920257    23761445    28004739    25244985    28648823    18362917    19820115    23537049    24508170    30249741    29240929    9214647    9214646    16204181    27762356    24755649    20798317    23254933    20433749    24297900    25069045    8605878    8901561    1454522    9512418    9851868    10779356    16369567   

EMBL

AGBW02008904    OWR52231.1    ODYU01012011    SOQ58075.1    NWSH01000348    PCG77182.1    + More
KQ461073    KPJ09565.1    KQ459053    KPJ04044.1    JXUM01024334    JXUM01024335    JXUM01087698    JXUM01087699    KQ563653    KQ560687    KXJ73554.1    KXJ81271.1    CH477637    EAT37976.1    BT021329    AAX33477.1    GFDL01015765    JAV19280.1    GDHF01000205    JAI52109.1    GAKP01007211    JAC51741.1    DS232126    EDS35571.1    JRES01000780    KNC28392.1    CH954177    EDV59679.1    CM000157    EDW89190.1    CH902619    EDV37871.1    GBXI01006639    JAD07653.1    GAMC01001959    JAC04597.1    CM002911    KMY92000.1    CH480816    EDW46767.1    AAAB01008948    EAA10355.3    APCN01004657    AE013599    AHN55958.1    S80255    CH933808    EDW09225.1    CH940648    EDW61876.1    GBYB01015313    JAG85080.1    KF471133    AHA42533.1    CP012524    ALC40873.1    AXCM01003001    CM000071    EAL25984.1    PYGN01002995    PSN29765.1    KK852792    KDR16461.1    CH963719    EDW71975.1    ATLV01023101    KE525340    KFB48455.1    CCAG010007472    CH920114    EDV90433.1    ADMH02000108    ETN67810.1    OUUW01000001    SPP75703.1    GEBQ01025966    JAT14011.1    GECZ01025975    JAS43794.1    GEZM01085192    JAV60140.1    KA645406    AFP60035.1    CH479223    EDW35043.1    CH916367    EDW02583.1    NNAY01001603    OXU23451.1    KQ971352    EFA06648.2    AXCN02000977    APGK01025879    KB740605    ENN80029.1    LBMM01000833    KMQ97399.1    NEVH01024950    PNF16400.1    KK107151    QOIP01000003    EZA57318.1    RLU25202.1    UFQS01000188    UFQT01000188    SSX00981.1    SSX21361.1    QCYY01001161    ROT80053.1    CVRI01000030    CRK92530.1    U44049    BC083031    AAC60225.1    AAH83031.1    CM004477    OCT74714.1    FR904442    CDQ63477.1    BC074670    AAH74670.1    GL437781    EFN69966.1    GL451850    EFN78411.1    KB201262    ESO98208.1    PPHD01022692    POI27685.1    GCES01027262    JAR59061.1    BT059391    ACN11104.1    CP026249    AWP03956.1    AZIM01000957    ETE68700.1    IACM01111057    LAB33628.1    CM004476    OCT76788.1    U29178    U46131    BC072870    Z15033    KK714268    KFQ35316.1   

Proteomes

UP000007151    UP000218220    UP000053240    UP000053268    UP000069940    UP000249989    + More
UP000008820    UP000002320    UP000095300    UP000095301    UP000078200    UP000037069    UP000008711    UP000002282    UP000007801    UP000075903    UP000075902    UP000001292    UP000007062    UP000076407    UP000075882    UP000075840    UP000075880    UP000000803    UP000009192    UP000192221    UP000075884    UP000075901    UP000008792    UP000075920    UP000192223    UP000075900    UP000075885    UP000002358    UP000092553    UP000075883    UP000001819    UP000245037    UP000027135    UP000007798    UP000030765    UP000092444    UP000001070    UP000000673    UP000268350    UP000075881    UP000008744    UP000076408    UP000069272    UP000215335    UP000007266    UP000075886    UP000019118    UP000036403    UP000235965    UP000053097    UP000279307    UP000283509    UP000183832    UP000192220    UP000264800    UP000192224    UP000261540    UP000087266    UP000186698    UP000265040    UP000193380    UP000008143    UP000000311    UP000008237    UP000030746    UP000265020    UP000246464    UP000265140    UP000261420   

PRIDE

Q5BI95    Q26454    Q5XK83    Q6GL41    P30664   

Pfam

PF00493   MCM        + More
PF17207   MCM_OB
PF17855   MCM_lid
PF14551   MCM_N
PF01448   ELM2
PF02944   BESS

Interpro

IPR008047   MCM_4        + More
IPR018525   MCM_CS       
IPR027925   MCM_N       
IPR041562   MCM_lid       
IPR027417   P-loop_NTPase       
IPR033762   MCM_OB       
IPR031327   MCM       
IPR001208   MCM_dom       
IPR012340   NA-bd_OB-fold       
IPR003593   AAA+_ATPase       
IPR001005   SANT/Myb       
IPR017884   SANT_dom       
IPR009057   Homeobox-like_sf       
IPR000949   ELM2_dom       
IPR004210   BESS_motif       
IPR006578   MADF-dom       

SUPFAM

SSF52540   SSF52540        + More
SSF50249   SSF50249       
SSF46689   SSF46689       

ProteinModelPortal

A0A212FER1   A0A2H1WYC1   A0A2A4K0L3   A0A194QX82   A0A194QF49   A0A182H304   + More
Q16TZ0   Q5BI95   A0A1Q3EVE7   A0A0K8WM27   A0A034W864   B0WVG5   A0A1I8QAN5   A0A1I8N9V2   A0A1A9USP5   A0A0L0C837   B3N9Y3   B4P1Q4   B3MGV3   A0A0A1XAF5   A0A182VI32   A0A182TDY8   W8C9Q3   A0A0J9TVC7   B4HQV7   Q7PPI5   A0A182XEH1   A0A182KNP9   A0A182HQL4   A0A182ILA0   A0A0B4LEV2   Q26454   B4KQ97   A0A1W4VDK0   A0A182N1Y6   A0A182T370   B4LLC4   A0A0C9QKK7   A0A182WM22   A0A1W4XPI5   A0A182RE53   V5KX69   A0A182PTV7   K7INC3   A0A0M3QUM2   A0A182M1N7   Q28YI1   A0A2P8XCP1   A0A067R0Z3   B4MIN6   A0A084WE11   A0A1B0G5U6   B4K2W4   W5JWV7   A0A3B0J6N2   A0A182JVZ5   A0A1B6KRG0   A0A1B6F0W3   A0A1Y1KIS5   T1P9F1   B4H8K8   A0A182YR51   B4J9Z6   A0A182FLN8   A0A232EYL2   D6WSI2   A0A182Q843   N6THZ9   A0A0J7L4H7   A0A2J7PJ85   A0A026WMZ8   A0A336K914   A0A3R7PR63   A0A1J1HY95   A0A2I4CZX7   A0A3Q2ZSX3   A0A1W5A084   A0A3B3RBL6   A0A1S3LXE0   Q5XK83   A0A1L8FSY2   A0A3Q1HY34   A0A060W8M7   Q6GL41   E2A9C4   E2C0X5   V4A2X0   A0A2P4SU88   A0A146YY99   C0HAN5   A0A3Q2E9N7   A0A2U9BJR0   V8P321   A0A2D4MKN7   A0A3P9AIQ8   A0A3B4V4A7   A0A1L8FYX1   P30664   A0A091R783  

PDB

6HV9     E-value=4.83071e-32,     Score=344

KEGG

105841783    no KO assigned | (RefSeq) DNA replication licensing factor MCM4-like

GO

GO:0042555   GO:0005524   GO:0003678   GO:0003677   GO:0005634   GO:0006270   GO:0043138   GO:0007052   GO:0003697   GO:0006268   GO:0006271   GO:0006267   GO:0000727   GO:0003688   GO:1902975   GO:0006260   GO:0004386   GO:0003682   GO:0001878   GO:0007049   GO:0046872   GO:0030174   GO:0000785   GO:0015935   GO:0006811   GO:0005622   GO:0006810   GO:0003824  

PANTHER

PTHR11630:SF66   PTHR11630  

Subcellular location

Nucleus  

Length:

358

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.00126

Exp number, first 60 AAs:

0

Total prob of N-in:

0.01388

outside

1  -  358

 

 

Pi

288.645183

Theta

171.762322

Tajima's D

1.794012

CLR

0.411089

CSRT

0.846207689615519

Interpretation

Uncertain