Gene

KWMTBOMO10934

Annotation

PREDICTED:_mitochondrial_tRNA-specific_2-thiouridylase_1_[Bombyx_mori]

Location

Bomo_Chr18(-):7458942-7460822      View in EpiBrowser

Full name

Mitochondrial tRNA-specific 2-thiouridylase 1      

Location in the cell

Cytoplasmic   Reliability : 2.418

CDS

ATGTTTCCTCTTGCCAATTTATTAAAAACTGAAGTGAGAGAAATTGCTAAAAAGGAAGGTCTTCTAAATGTAGCGAATAAAAAAGATAGCACAGGAATATGCTTCATAGGTAAAAAAAAGTTTCAAGATTTTATTGCAGAATACATTGATACAAAAAAAGGACAATTCATAGATATAGATACTGGAAGGATTGTTGGTGTACACGAAGGTTTGCACAAGTGGACAGTAGGTCAAAGATGTTGCCTGCCCAATTGTAGAGATGCATATTTTGTATTGAAGAAAGACTTGGCAACGAATAACATTTATGTGGTTGCAGGAACAAAACATTCAGCATTGTGGAACAATATTTGCATCACTATGCCACCTCACTGGATTAGGAGAGAACCTCTTGAATTAACAGAAAATGGGGTGCTTAATTGTTACTTTAGGTTCCAGCATACAAAACCTTTAGCTCCATGCAAAACTGTGAAAAATTCCGAGGGACTTACTATCATCTTAAAAAATAATTTAAGAGCAATTACTGAAGGACAATATGCAGTACTCTATAAAGATGGAGAATGTCTTGGTAGTGCCAAAATGAAAAACATTTGTCACAATTTTGTTTTTTAA

Protein

MFPLANLLKTEVREIAKKEGLLNVANKKDSTGICFIGKKKFQDFIAEYIDTKKGQFIDIDTGRIVGVHEGLHKWTVGQRCCLPNCRDAYFVLKKDLATNNIYVVAGTKHSALWNNICITMPPHWIRREPLELTENGVLNCYFRFQHTKPLAPCKTVKNSEGLTIILKNNLRAITEGQYAVLYKDGECLGSAKMKNICHNFVF

Description

Catalyzes the 2-thiolation of uridine at the wobble position (U34) of mitochondrial tRNA(Lys), tRNA(Glu) and tRNA(Gln). Required for the formation of 5-taurinomethyl-2-thiouridine (tm5s2U) of mitochondrial tRNA(Lys), tRNA(Glu), and tRNA(Gln) at the wobble position. ATP is required to activate the C2 atom of the wobble base.

Catalytic Activity

5-taurinomethyluridine(34) in tRNA + AH2 + ATP + S-sulfanyl-L-cysteinyl-[protein] = 5-taurinomethyl-2-thiouridine(34) in tRNA + A + AMP + diphosphate + H(+) + L-cysteinyl-[protein]

Miscellaneous

During the reaction, ATP is used to activate the C2 atom of U34 by adenylation. After this, the persulfide sulfur on the catalytic cysteine is transferred to the C2 atom of the wobble base (U34) of mitochondrial tRNA(Lys), tRNA(Glu) and tRNA(Gln). The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards the activated C2 atom on U34. Subsequently, a transient disulfide bond is formed between the two active site cysteine residues (By similarity).

Similarity

Belongs to the MnmA/TRMU family.

Keywords

ATP-binding   Complete proteome   Disulfide bond   Mitochondrion   Nucleotide-binding   Reference proteome   RNA-binding   Transferase   tRNA processing   tRNA-binding  

Feature

chain  Mitochondrial tRNA-specific 2-thiouridylase 1

Uniprot

A0A2W1BRV0   A0A2H1VJ37   A0A2A4K4S1   A0A3S2NRE3   A0A194PGN6   A0A194RQD2   + More
D6WSD8   A0A0N8CG99   A0A0P5T4C4   A0A0P6JLT6   A0A0P6B286   A0A0P5JAY7   A0A151XHF7   A0A154PQR8   A0A0L0CFM8   A0A195DJZ1   A0A2P8YGY5   A0A1I8P8S0   A0A1I8P8N8   A0A195C187   A0A195C2E6   A0A034WMB2   A0A151I1A0   N6TZ07   B4PXK2   A0A0K8VZ24   E9HGG5   A0A026VY94   Q9W5B6   A0A1Y1MTA8   A0A3L8D5W4   Q9U1L1   B4R2L6   A0A1J1IHL9   B3P9D2   A0A1I8MS83   B4I993   Q16EN5   Q170F2   K7J2F8   A0A232F4W9   A0A034WJT0   A0A2J7QWJ3   B0WBE3   A0A3R7PHY2   W8BN71   A0A1B6EMU6   A0A0K8TY76   A0A182GR64   W8C305   A0A2A3EAR4   A0A2M4CJ95   E2AFC4   A0A2T7NUH3   A0A1A9YMF3   E2C8U0   A0A0L7R3U6   A0A0V0GCD0   A0A1B3PDJ2   A0A1B6M3Q8   A0A0P4WJK1   A0A1B0D5K8   A0A182FV46   A0A1B6CLL6   A0A2M4CIT6   A0A1B0BDJ6   B3MYE9   A0A1B6DM22   A0A1B6C1U3   A0A3B0KYD4   A0A088AQD9   B4GTW3   A0A336KEP6   A0A224XLT6   B4L3S8   B5DNY9   A0A182TGC5   A0A0C9RVE2   Q7PZK6   A0A1S4H7Z4   A0A182HHP2   E9IA08   A0A182KAV0   A0A182L5A7   A0A0M5JA76   A0A182VK43   A0A1B0G8P8   A0A182XJN7   A0A1A9WVN2   A0A182QHX9   A0A182WE34   A0A2R7X028   B4JKU9   A0A182Y9K8   A0A1A9ZKS9   A0A182SUK1   B4N1Q7   A0A182N2Q0   A0A1W4VS81   A0A1B6C755  

EC Number

2.8.1.14

Pubmed

28756777    26354079    18362917    19820115    26108605    29403074    + More
25348373    23537049    17994087    17550304    21292972    24508170    10731132    12537572    12537569    28004739    30249741    25315136    17510324    20075255    28648823    24495485    26483478    20798317    15632085    12364791    21282665    20966253    25244985   

EMBL

KZ149920    PZC77768.1    ODYU01002848    SOQ40838.1    NWSH01000165    PCG78908.1    + More
RSAL01000123    RVE46635.1    KQ459604    KPI92556.1    KQ459765    KPJ20073.1    KQ971352    EFA07642.2    GDIP01134655    JAL69059.1    GDIP01131221    LRGB01001005    JAL72493.1    KZS14165.1    GDIQ01014631    JAN80106.1    GDIP01022388    JAM81327.1    GDIQ01227664    JAK24061.1    KQ982130    KYQ59844.1    KQ435036    KZC14077.1    JRES01000455    KNC31046.1    KQ980800    KYN12809.1    PYGN01000601    PSN43522.1    KQ978379    KYM94385.1    KYM94386.1    GAKP01004024    JAC54928.1    KQ976599    KYM79517.1    APGK01045809    APGK01045810    KB741039    ENN74530.1    CM000162    EDX00855.1    GDHF01014660    GDHF01008215    JAI37654.1    JAI44099.1    GL732641    EFX69171.1    KK107597    EZA48605.1    AE014298    AY051773    GEZM01024758    JAV87725.1    QOIP01000013    RLU15403.1    AL109630    CAB65878.1    CM000366    EDX16816.1    CVRI01000052    CRK99759.1    CH954183    EDV45428.1    CH480825    EDW43774.1    CH478641    EAT32694.1    EAT32696.1    EJY58120.1    CH477475    EAT40320.1    NNAY01000959    OXU25725.1    GAKP01004023    JAC54929.1    NEVH01009430    PNF32963.1    DS231879    EDS42382.1    QCYY01002846    ROT67123.1    GAMC01006303    JAC00253.1    GECZ01030538    JAS39231.1    GDHF01032880    JAI19434.1    JXUM01081860    KQ563273    KXJ74144.1    GAMC01006299    JAC00257.1    KZ288317    PBC28299.1    GGFL01001053    MBW65231.1    GL439108    EFN67817.1    PZQS01000009    PVD24838.1    GL453694    EFN75657.1    KQ414661    KOC65560.1    GECL01000630    JAP05494.1    KU659999    AOG17797.1    GEBQ01009447    JAT30530.1    GDRN01067963    GDRN01067961    GDRN01067960    GDRN01067959    JAI64279.1    AJVK01025409    GEDC01026878    GEDC01023117    JAS10420.1    JAS14181.1    GGFL01001078    MBW65256.1    JXJN01012503    CH902632    EDV32643.1    GEDC01010574    JAS26724.1    GEDC01029830    GEDC01015228    JAS07468.1    JAS22070.1    OUUW01000018    SPP89108.1    CH479190    EDW25983.1    UFQS01000328    UFQT01000328    SSX02921.1    SSX23288.1    GFTR01005638    JAW10788.1    CH933810    EDW07206.2    CH379065    EDY73241.1    GBYB01012870    JAG82637.1    AAAB01008986    EAA00244.3    APCN01002431    GL761885    EFZ22595.1    CP012528    ALC48161.1    CCAG010000299    AXCN02001081    KK856153    PTY25041.1    CH916370    EDW00202.1    CH963925    EDW78296.1    GEDC01027975    JAS09323.1   

Proteomes

UP000218220    UP000283053    UP000053268    UP000053240    UP000007266    UP000076858    + More
UP000075809    UP000076502    UP000037069    UP000078492    UP000245037    UP000095300    UP000078542    UP000078540    UP000019118    UP000002282    UP000000305    UP000053097    UP000000803    UP000279307    UP000000304    UP000183832    UP000008711    UP000095301    UP000001292    UP000008820    UP000002358    UP000215335    UP000235965    UP000002320    UP000283509    UP000069940    UP000249989    UP000242457    UP000000311    UP000245119    UP000092443    UP000008237    UP000053825    UP000092462    UP000069272    UP000092460    UP000007801    UP000268350    UP000005203    UP000008744    UP000009192    UP000001819    UP000075902    UP000007062    UP000075840    UP000075881    UP000075882    UP000092553    UP000075903    UP000092444    UP000076407    UP000091820    UP000075886    UP000075920    UP000001070    UP000076408    UP000092445    UP000075901    UP000007798    UP000075884    UP000192221   

PRIDE

Q9W5B6    Q9U1L1   

Pfam

PF00085   Thioredoxin

Interpro

IPR023382   Adenine_a_hdrlase_dom        + More
IPR004506   tRNA-specific_2-thiouridylase       
IPR014729   Rossmann-like_a/b/a_fold       
IPR036249   Thioredoxin-like_sf       
IPR041862   ERp44_PDI_b'       
IPR041870   ERp44_PDI_b       
IPR013766   Thioredoxin_domain       

SUPFAM

SSF52833   SSF52833       

Gene 3D

2.30.30.280     3.40.50.620    

CDD

cd01998  tRNA_Me_trans
cd03072  PDI_b'_ERp44
cd03070  PDI_b_ERp44

ProteinModelPortal

A0A2W1BRV0   A0A2H1VJ37   A0A2A4K4S1   A0A3S2NRE3   A0A194PGN6   A0A194RQD2   + More
D6WSD8   A0A0N8CG99   A0A0P5T4C4   A0A0P6JLT6   A0A0P6B286   A0A0P5JAY7   A0A151XHF7   A0A154PQR8   A0A0L0CFM8   A0A195DJZ1   A0A2P8YGY5   A0A1I8P8S0   A0A1I8P8N8   A0A195C187   A0A195C2E6   A0A034WMB2   A0A151I1A0   N6TZ07   B4PXK2   A0A0K8VZ24   E9HGG5   A0A026VY94   Q9W5B6   A0A1Y1MTA8   A0A3L8D5W4   Q9U1L1   B4R2L6   A0A1J1IHL9   B3P9D2   A0A1I8MS83   B4I993   Q16EN5   Q170F2   K7J2F8   A0A232F4W9   A0A034WJT0   A0A2J7QWJ3   B0WBE3   A0A3R7PHY2   W8BN71   A0A1B6EMU6   A0A0K8TY76   A0A182GR64   W8C305   A0A2A3EAR4   A0A2M4CJ95   E2AFC4   A0A2T7NUH3   A0A1A9YMF3   E2C8U0   A0A0L7R3U6   A0A0V0GCD0   A0A1B3PDJ2   A0A1B6M3Q8   A0A0P4WJK1   A0A1B0D5K8   A0A182FV46   A0A1B6CLL6   A0A2M4CIT6   A0A1B0BDJ6   B3MYE9   A0A1B6DM22   A0A1B6C1U3   A0A3B0KYD4   A0A088AQD9   B4GTW3   A0A336KEP6   A0A224XLT6   B4L3S8   B5DNY9   A0A182TGC5   A0A0C9RVE2   Q7PZK6   A0A1S4H7Z4   A0A182HHP2   E9IA08   A0A182KAV0   A0A182L5A7   A0A0M5JA76   A0A182VK43   A0A1B0G8P8   A0A182XJN7   A0A1A9WVN2   A0A182QHX9   A0A182WE34   A0A2R7X028   B4JKU9   A0A182Y9K8   A0A1A9ZKS9   A0A182SUK1   B4N1Q7   A0A182N2Q0   A0A1W4VS81   A0A1B6C755  

PDB

2DEU     E-value=2.31008e-27,     Score=300

KEGG

101746009    K21027 tRNA-5-taurinomethyluridine 2-sulfurtransferase [EC:2.8.1.14] | (RefSeq) mitochondrial tRNA-s

GO

GO:0008033   GO:0016783   GO:0005739   GO:0002143   GO:0000049   GO:0005524   GO:0008168   GO:0045454   GO:0005623   GO:0006396   GO:0003723   GO:0016075   GO:0005839   GO:0051603   GO:0051028   GO:0005622   GO:0006810   GO:0003824  

Subcellular location

Mitochondrion  

Length:

202

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.00617

Exp number, first 60 AAs:

0

Total prob of N-in:

0.01975

outside

1  -  202

 

 

Pi

157.366853

Theta

185.060735

Tajima's D

-0.648471

CLR

132.840239

CSRT

0.210939453027349

Interpretation

Uncertain