Gene

KWMTBOMO10930

Pre Gene Modal

BGIBMGA008342

Annotation

PREDICTED:_NEDD8-activating_enzyme_E1_regulatory_subunit_[Papilio_machaon]

Location

Bomo_Chr18(-):7410022-7411782      View in EpiBrowser

Full name

NEDD8-activating enzyme E1 regulatory subunit      

Alternative Name

APP-BP1
Amyloid protein-binding protein 1

Location in the cell

Cytoplasmic   Reliability : 3.488

CDS

ATGGCGTCTCCATCTCCTAAATCACCAGAACAAAGCGAGAAGAATAAACAGTACGACCGGCAACTTCGTTTGTGGGGTGATCACGGACAAAGTGCTCTTGAAAGCGGACACATATGTTTAATTAATGCTACTGCCTTGGGTACTGAAATATTGAAATCGATAGTTCTTCCAGGAATTGGTGCAATTACTATAGTTGATGATTGCACAGTTAGTGATGAAGATATAGGCAGTAATTTCTTTTTGGAAATGTCTAGCAAAGGTCTTAACAGAGGATCCGAAGCGTTAAGGCTTGTTTTAGAATTAAATCCAGCAGTACAAGGTCATGCAGTCCAAGAACCACCTGACCAGATACTTGATGAGAACCCAGACTTTTTTAAATCATTCAGTTTAGTGATAGCTACTACAATAAGTGAAAAAGTAACGCAAAGTTTATCTCAACACCTGTGGGATATAAATGTTCCTTTTATACTTTGCCAATCAGTTGGATTCTTAGGGTCTTTAAGAATTCAGATTCGGGAACATGCTGTTACTGAAACTCATCCTGATAATGAACAAACAGATCTGCGCCTAGATGAACCATTTATGTCATTGTTAGAACATCTGGAAAGTATTGATATTGATGCTCTTGATTTGAAAGATCATGGTCACATTCCTTGGATTATAATTCTTTATAAAGCTGTTCAAAAGTGGAAAATTAACAATAACAATAAATGGCCTGTAACGCGAAATGATAAAGAAGATATAAAAAATATAATAAGGGATTTCATTAGAAAAGATGAAAATGGAATACCAATTAATGAAGAGAATTTTGAGGAAGCAATAAGAGCTGTTAACACTGCTTTGATGCCTACATTTTTGCCTAAGAATATCTCAAAACTTATGTATAATAGTGCTGCCACAAATTTAACTAAGGATAGTTCATCATTTTGGATTATGTGCTCTGCCTTAAGAGCATTTGTTGAGGCTGAAGGGAAGGGAAAACTTCCAGTGAAAGGAATCTTACCTGATATGACGGCCTCTACAGAACATTATGTAAAACTTCAAAACATTTACAAGATGCAAGCTGCAAATGATGCAGAAATAGTCTATAGAAAAGTTCGACAGATAGTCACTCAATTGCATTGTGATAGTATTAGTGAGGCAGATGTTAAACTATTCTGCAGACATGCTCATGATTTATATTTGGTGAAGGGGTCTTGTGTGGCAACAGAACTTCAACTGGGAAATTCTGTTGCATCCTATATAGCCACATATTTAGAGGAGCCAGATGTCATGATGGTTCACTATATTATGCTTCGGGCAGCTGGTATGTTTAGATCAGAGCACTGCCGAGCTCCCGGTGATTGGGAACCAGAAGGTGACATTGCTAAGCTAAAAACTTGTGTAAATAAACTTTTAGGTGATATATCTTGTTCTCCATTTCCTAAAGATGATCATATCCACGAAATGTGTCGCTATGGGGGAGCCGAGATACATAGTGTGTCTGCCTTTTTAGGTGGATGTGTTGCACATGAAGCCATTAAAATTATAACCAAGCAATATAAACCTGTAAATAATACATTTATTTATGATGGAGCTTCTACAAATACGGCAACATTTGAATTCTAA

Protein

MASPSPKSPEQSEKNKQYDRQLRLWGDHGQSALESGHICLINATALGTEILKSIVLPGIGAITIVDDCTVSDEDIGSNFFLEMSSKGLNRGSEALRLVLELNPAVQGHAVQEPPDQILDENPDFFKSFSLVIATTISEKVTQSLSQHLWDINVPFILCQSVGFLGSLRIQIREHAVTETHPDNEQTDLRLDEPFMSLLEHLESIDIDALDLKDHGHIPWIIILYKAVQKWKINNNNKWPVTRNDKEDIKNIIRDFIRKDENGIPINEENFEEAIRAVNTALMPTFLPKNISKLMYNSAATNLTKDSSSFWIMCSALRAFVEAEGKGKLPVKGILPDMTASTEHYVKLQNIYKMQAANDAEIVYRKVRQIVTQLHCDSISEADVKLFCRHAHDLYLVKGSCVATELQLGNSVASYIATYLEEPDVMMVHYIMLRAAGMFRSEHCRAPGDWEPEGDIAKLKTCVNKLLGDISCSPFPKDDHIHEMCRYGGAEIHSVSAFLGGCVAHEAIKIITKQYKPVNNTFIYDGASTNTATFEF

Description

Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M.
Regulatory subunit of the dimeric uba3-nae1 E1 enzyme. E1 activates nedd8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a nedd8-uba3 thioester and free AMP. E1 finally transfers nedd8 to the catalytic cysteine of ube2m (By similarity).

Subunit

Heterodimer of uba3 and nae1. The complex binds nedd8 and ube2m (By similarity).

Similarity

Belongs to the ubiquitin-activating E1 family. ULA1 subfamily.

Keywords

Ubl conjugation pathway   Alternative splicing   Complete proteome   Reference proteome  

Feature

chain  NEDD8-activating enzyme E1 regulatory subunit
splice variant  In isoform 2.

Uniprot

H9JFP5   A0A2A4K5E5   A0A2H1WS32   A0A2W1C107   A0A194RQD7   A0A194PGP9   + More
A0A212FCA1   A0A0L7KU08   A0A026WUW0   A0A1B6EM87   A0A2J7REN5   E9IAZ2   A0A067RIK4   K7IP66   A0A154P9P5   E2AY86   A0A1B6BZM3   A0A0C9QMW4   A0A232FA34   A0A151XDN3   E2B689   A0A151IL80   A0A0M8ZUW6   A0A151IYU0   A0A146LYI4   A0A158P3I5   E0W0X0   A0A151JXD6   A0A0L7RDR6   A0A0V0G5L6   A0A224XN02   A0A2A3E8Y8   A0A0P4VKL2   T1I595   R4G5L8   A0A1S3CZ93   J9K171   A0A131Z5N2   L7MAB5   D6WGG4   A0A2S2PFJ2   A0A224YJJ3   A0A131XH62   A0A131XSV5   A0A3P9JD45   A0A0P4VXZ9   A0A147BSN8   A0A3P9L0Q9   H2LLC6   A0A1E1X2X7   A0A3B5B099   A0A182GEJ2   A0A1W4ZWL0   F4WB76   A0A3Q3R0L6   A0A3Q3KPK7   A0A1E1XSX6   G3MPB9   A0A1W5A5M5   A0A336MK03   A0A3B3DS61   A0A3B4WD32   A0A336LIK4   A0A023GN81   A0A3Q2ZBP2   A0A2K6VB61   J7M297   A0A3S2PRV1   A0A3Q1I6M6   A0A2U9BBW2   A0A1Q3FA90   B0X7Y7   A0A084WKK0   A0A3Q1C1Y7   A0A2M4CYD2   Q7Q726   Q6NTW6   A0A1L8GEL9   A0A1J1IPP2   Q170Z9   A0A182LJF3   A0A3P8TPC3   Q7SXP2   B0XB59   A0A182MK24   A0A182XUF7   A0A3Q2PTC9   A0A1S3KTD2   A0A2R5LCJ3   F1QR83   I3J4P7   B7ZV51   B0BME5   H2V4P7   A0A3P9AZQ1   A0A3Q2WBR0   Q16N42   A0A3B4F5T3   A0A3P8ULS9  

Pubmed

19121390    28756777    26354079    22118469    26227816    24508170    + More
30249741    21282665    24845553    20075255    20798317    28648823    26823975    21347285    20566863    27129103    26830274    25576852    18362917    19820115    28797301    28049606    17554307    29652888    28503490    26483478    21719571    29209593    22216098    29451363    20920257    24438588    12364791    14747013    17210077    27762356    17510324    20966253    23594743    25186727    20431018    21551351    24487278   

EMBL

BABH01001984    NWSH01000165    PCG78902.1    ODYU01010245    SOQ55224.1    KZ149920    + More
PZC77763.1    KQ459765    KPJ20078.1    KQ459604    KPI92561.1    AGBW02009208    OWR51362.1    JTDY01005869    KOB66534.1    KK107088    QOIP01000008    EZA59802.1    RLU19506.1    GECZ01030746    JAS39023.1    NEVH01004959    PNF39299.1    GL762091    EFZ22238.1    KK852482    KDR22868.1    KQ434842    KZC08114.1    GL443762    EFN61621.1    GEDC01030586    JAS06712.1    GBYB01004869    GBYB01004870    JAG74636.1    JAG74637.1    NNAY01000633    OXU27293.1    KQ982268    KYQ58463.1    GL445930    EFN88782.1    KQ977133    KYN05489.1    KQ435840    KOX71457.1    KQ980740    KYN13719.1    GDHC01006400    JAQ12229.1    ADTU01008083    DS235862    EEB19276.1    KQ981604    KYN39717.1    KQ414612    KOC69132.1    GECL01002764    JAP03360.1    GFTR01007025    JAW09401.1    KZ288344    PBC27666.1    GDKW01000968    JAI55627.1    ACPB03000705    GAHY01000420    JAA77090.1    ABLF02040187    GEDV01002269    JAP86288.1    GACK01004956    JAA60078.1    KQ971321    EFA00552.1    GGMR01015590    MBY28209.1    GFPF01005979    MAA17125.1    GEFH01003153    JAP65428.1    GEFM01006488    JAP69308.1    GDRN01104620    JAI57881.1    GEGO01001620    JAR93784.1    GFAC01005563    JAT93625.1    JXUM01242700    KQ635604    KXJ62460.1    GL888058    EGI68549.1    GFAA01001042    JAU02393.1    JO843720    AEO35337.1    UFQS01000673    UFQT01000673    SSX06031.1    SSX26388.1    UFQS01004372    UFQT01004372    SSX16351.1    SSX35668.1    GBBM01000815    JAC34603.1    AB665323    BAM36364.1    CM012439    RVE74384.1    CP026247    AWP01443.1    GFDL01010606    JAV24439.1    DS232468    EDS42206.1    ATLV01024123    KE525349    KFB50744.1    GGFL01006097    MBW70275.1    AAAB01008960    EAA11771.2    BC068838    CM004473    OCT82328.1    CVRI01000057    CRL02213.1    CH477462    EAT40545.1    BC050171    BC055513    BC151887    DS232614    EDS44112.1    AXCM01007885    GGLE01003117    MBY07243.1    BX511136    AERX01013865    BC171459    AAI71459.1    AAMC01025501    BC158396    AAI58397.1    CH477841    EAT35744.1   

Proteomes

UP000005204    UP000218220    UP000053240    UP000053268    UP000007151    UP000037510    + More
UP000053097    UP000279307    UP000235965    UP000027135    UP000002358    UP000076502    UP000000311    UP000215335    UP000075809    UP000008237    UP000078542    UP000053105    UP000078492    UP000005205    UP000009046    UP000078541    UP000053825    UP000242457    UP000015103    UP000079169    UP000007819    UP000007266    UP000265200    UP000265180    UP000001038    UP000261400    UP000069940    UP000249989    UP000192224    UP000007755    UP000261600    UP000261640    UP000261560    UP000261360    UP000264800    UP000265040    UP000246464    UP000002320    UP000030765    UP000257160    UP000007062    UP000186698    UP000183832    UP000008820    UP000075882    UP000265080    UP000000437    UP000075883    UP000076407    UP000265000    UP000087266    UP000005207    UP000008143    UP000005226    UP000265160    UP000264840    UP000261460    UP000265120   

PRIDE

H9JFP5   

Pfam

PF00899   ThiF

Interpro

IPR030667   APP-BP1        + More
IPR000594   ThiF_NAD_FAD-bd       
IPR035985   Ubiquitin-activating_enz       

SUPFAM

SSF69572   SSF69572       

ProteinModelPortal

H9JFP5   A0A2A4K5E5   A0A2H1WS32   A0A2W1C107   A0A194RQD7   A0A194PGP9   + More
A0A212FCA1   A0A0L7KU08   A0A026WUW0   A0A1B6EM87   A0A2J7REN5   E9IAZ2   A0A067RIK4   K7IP66   A0A154P9P5   E2AY86   A0A1B6BZM3   A0A0C9QMW4   A0A232FA34   A0A151XDN3   E2B689   A0A151IL80   A0A0M8ZUW6   A0A151IYU0   A0A146LYI4   A0A158P3I5   E0W0X0   A0A151JXD6   A0A0L7RDR6   A0A0V0G5L6   A0A224XN02   A0A2A3E8Y8   A0A0P4VKL2   T1I595   R4G5L8   A0A1S3CZ93   J9K171   A0A131Z5N2   L7MAB5   D6WGG4   A0A2S2PFJ2   A0A224YJJ3   A0A131XH62   A0A131XSV5   A0A3P9JD45   A0A0P4VXZ9   A0A147BSN8   A0A3P9L0Q9   H2LLC6   A0A1E1X2X7   A0A3B5B099   A0A182GEJ2   A0A1W4ZWL0   F4WB76   A0A3Q3R0L6   A0A3Q3KPK7   A0A1E1XSX6   G3MPB9   A0A1W5A5M5   A0A336MK03   A0A3B3DS61   A0A3B4WD32   A0A336LIK4   A0A023GN81   A0A3Q2ZBP2   A0A2K6VB61   J7M297   A0A3S2PRV1   A0A3Q1I6M6   A0A2U9BBW2   A0A1Q3FA90   B0X7Y7   A0A084WKK0   A0A3Q1C1Y7   A0A2M4CYD2   Q7Q726   Q6NTW6   A0A1L8GEL9   A0A1J1IPP2   Q170Z9   A0A182LJF3   A0A3P8TPC3   Q7SXP2   B0XB59   A0A182MK24   A0A182XUF7   A0A3Q2PTC9   A0A1S3KTD2   A0A2R5LCJ3   F1QR83   I3J4P7   B7ZV51   B0BME5   H2V4P7   A0A3P9AZQ1   A0A3Q2WBR0   Q16N42   A0A3B4F5T3   A0A3P8ULS9  

PDB

1YOV     E-value=5.8486e-144,     Score=1311

KEGG

101736100    K04532 amyloid beta precursor protein binding protein 1 | (RefSeq) NEDD8-activating enzyme E1 regula

GO

GO:0019781   GO:0045116   GO:0005737   GO:0032446   GO:0008641   GO:0004298   GO:0005839   GO:0051603   GO:0051028   GO:0005622   GO:0006810   GO:0003824  

Length:

535

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.02674

Exp number, first 60 AAs:

0.00433

Total prob of N-in:

0.00070

outside

1  -  535

 

 

Pi

118.877245

Theta

200.533811

Tajima's D

-1.526547

CLR

2983.017664

CSRT

0.0607469626518674

Interpretation

Uncertain