Gene

KWMTBOMO10816  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA008442

Annotation

PREDICTED:_pyruvate_carboxylase?_mitochondrial_isoform_X1_[Bombyx_mori]

Location

Bomo_Chr18(+):3648845-3686034      View in EpiBrowser

Full name

Pyruvate carboxylase      

Location in the cell

Cytoplasmic   Reliability : 1.677 Mitochondrial   Reliability : 1.974

CDS

ATGCAAATACTAAAGGCAAGATTCGCGTTACGCGCCACAGCGTCGCAATTCCAGGCATGGAGCTCGGCCAAAACTCGGACAGCTGCTACGGACTCGAAGATAGTGGAATACAAACCAATCAGAAGCGTTCTTGTTGCGAATCGCGGCGAAATCGCCATACGTGTGTTCCGAGCTTGCACGGAACTAGGCATCCGTTCTGTGGCTATTTACAGCGAACAAGACAAACTGCAAATGCACAGACAAAAAGCCGACGAATCGTATATAGTCGGAAAAGGTCTGCCACCAGTCGAGGCCTACCTCAGCATCCCGGAGATCATCAGGGTTGCGAAGGAGAACGACGTTGACGCCGTACATCCCGGCTACGGTCTTTTATCTGAAAGGGCCGATTTCGCCCAGGCCATCATTAATGCCGGTCTGCGGTTCATCGGGCCCTCGCCGAAGGTCGTGCAGCAGATGGGAGACAAAGTAGCCGCCAGAAAAGCTGCCATCGAAGCAAAGGTGCCGATTGTTCCCGGAACCGATGGTCCGGTCACAACGAAAGAGGAGGCTGTGGAGTTCTGCAAAAGTCACGGTCTTCCGGTTATCTTAAAGGCTGCGTACGGAGGCGGCGGTCGTGGTATGAGGGTGGTCCGTAAGATGGAAGAAGTGCCTTCGGCTTTCGAACGAGCTTCTTCAGAAGCTCTCGGCGCTTTCGGCAACGGTTCCATGTTTATCGAACGATTCATCGAACGACCCCGGCACATCGAAGTGCAGTTGTTGGGCGACAAAGCCGGCAACGTTGTGCATTTATACGAACGGGATTGTTCAGTACAGAGGAGACATCAGAAAGTCGTCGAAATTGCTCCTGCACCGAAACTGGACCCTAAGATTCGTCAGAAAATGGTCGAGTGCGCCACACATCTGGCGCGGCACGTCGGCTACGAGAACGCCGGCACGGTCGAGTTCCTGCTCGACGACAAGGGGAACTTCTACTTCATCGAGGTCAACGCCAGATTGCAAGTAGAGCATACCATAACAGAAGAAGTCACAGGTATCGACCTAGTACAGTCACAGATACGTATCGCCGAGGGGATGACCCTGCCCGAACTAGGGTTGACGCAGGATAAAATACAAGCACAGGGATACGCGATACAATGTCGTGTTACTACCGAAGATCCGGCCAATAACTTCCAGCCCAGTACGGGCAGGATCGAGGTGTTCAGATCCGGTGAAGGGATGGGAATCCGTCTCGACTCAGCCTCCACGTACGCCGGCGCCGTCATCTCTCCGTACTACGACTCTTTACTTGTCAAAGTGATCTCTCACGCTCACGACCTACCCTCGTCGGCGGCCAAAATGAATAGAGCCCTCCGCGAGTTCCGCATCCGAGGCGTCAAGACCAACATCCCCTTCTTACTCAACGTGCTCGAGAACCAGAAATTCCTAAACGGTTCGGTCGACACGTACTTCATAGACGAAAATCCGCAACTGTTCATGTTTAAAGCCTCACAGAACAGAGCGCAGAAGATCCTCAACTATCTTGGCTATGTGCTGGTCAACGGGCCGGCCACTCCTCTTGCCACCAAAATACCCCCATCTGACGTCAAGCCCTACATACCTCAAGTGCCGCTGGCCGTCCAACCGCCGAAGGGCTACAAACAAATACTCGACGAGGGTGGCCCCGAGGCCTTCGCCAAAGCGGTCCGGCAGCACAAGGGTCTACTCCTGATGGACACGACGTTCCGGGACGCGCACCAGTCGCTGCTGGCCACCAGGGTGCGGACCCACGACCTGCTCGCCGTCTCGCCCTACGTAGCGCACAACTTCAACAACCTGTACTCGCTCGAGAACTGGGGAGGAGCTACATTCGACGTGGCCTTGAGATTCCTCCACGAGTGTCCGTGGGAGCGTCTCCACGACATGAGGCAGCTGATCCCGAACATTCCGTTCCAGATGTTGCTCCGCGGCGCCAACGCGGTCGGTTACACCAACTATCCCGACAACGTTGTTTTCAAGTTCTGCGACATGGCCGTCCAAGCAGGAATGGACATCTTCCGAGTTTTCGATTCGCTGAACTACCTGCCGAACTTGGTACTGGGCATGGAGGCAGCCGCGAAGGCAGGCGGAGTCGTCGAGGCCGCCATCTCATACACGGGCGACGTTTCGGACCCCAGCAAAACTAAATACGACCTGAAGTATTACACGAAGCTGGCCGACGATCTGGTGAAGGCCGGAACTCACGTGCTCGCTATCAAAGACATGGCCGGACTGCTGAAGCCCCAGGCTGCGAAGTTGCTGATAGGCGCCATCCGCGCGGCGCACCCGCACGTCCCGCTGCACGTGCACACGCACGACACGAGCGGCGCGGGCGTGGCCGCCATGCTGGCGTGCGCGGAGGCGGGCGCGGACGTGGTGGACGTCGCCGTGGACTCCGTGTCCGGACTCACCTCGCAGCCCAGCATGGGCGCTCTGGTGGCTTCCTTGCAGGGCACCCCCTTGGACACTAAGATACCGCTACAAAAGGTGTCAGAGTACTCAGCCTATTGGGAGCAAGCTCGTACCCTATACGGACCCTTCGAATGTACGGCCACAATGAAATCTGGCAACGCTGATGTGTACCTCAATGAGATCCCCGGAGGACAGTACACCAATCTACAATTCCAAGCTTTCTCCTTAGGACTGGGAAGCCAATTCGAAGAGGTCAAGAAAGCTTACAGAGAAGCAAATCTCCTCTTGGGTGATATAATCAAAGTTACTCCATCGTCGAAGGTCGTGGGAGATCTTGCACAGTTCATGGTACAGAACAAGCTGACGGCTGCCGATATCAACGCTAGAGCTGAGGAGCTCTCCTTCCCGAAGTCGGTGGTGGAGTACCTGCAGGGAGCCATCGGGATACCGTATGGAGGATTCCCTGAACCATTGAGGTCGAAAGTTCTGAAAGACATGCCGAGAATCGAAGGACGTCCCGGAAAAGAGTTGCCTTCTCTGAACTTCGATAAACTAAAGGGTGAACTGAAAGAATCTTTCCCAGACATAAACGACGAGGACGTGATGTCGGCGGCGATGTACCCGCAGGTCGCGAGCGACTTCTTCCGTTTCCGGGACAAGTACGGGCCCGTCAAACATCTGGACACCAAGACCTTCCTGGTCGGCCCCGCGGTCGGCGACACCATAGAGGTTAAGATAGAGCGCGGCAAGACCCTGGACATCAGGACGGTGGCGGTGTCGGAGGACATGACGGCCGCGGGAGAGCGTGAGGTCTTCTTCGAGCTTAACGGACAACTGCGGTCAGTCTTCATCAGAGACGAAAACGCCAGCAAGGAAATGAAGATCCACCCGAAGGCGGTGAAAGGCGATAAGAATCAAGTGGGTGCCCCGATGCCCGGAACCGTTCTCACTATCAAAGTGAAGGAAGGCGACAAAGTCGAGAAGGGGCAGCCGATAGCCGTGCTCTCCGCTATGAAAATGGAAATGATCGTTCAAGCCCCCAAAGCTGGTACCGTCAAGACCGTCTCGATTACAAACGGACAAAAACTAGAAGGCGACGATTTGATCTGCACTATCGAATAA

Protein

MQILKARFALRATASQFQAWSSAKTRTAATDSKIVEYKPIRSVLVANRGEIAIRVFRACTELGIRSVAIYSEQDKLQMHRQKADESYIVGKGLPPVEAYLSIPEIIRVAKENDVDAVHPGYGLLSERADFAQAIINAGLRFIGPSPKVVQQMGDKVAARKAAIEAKVPIVPGTDGPVTTKEEAVEFCKSHGLPVILKAAYGGGGRGMRVVRKMEEVPSAFERASSEALGAFGNGSMFIERFIERPRHIEVQLLGDKAGNVVHLYERDCSVQRRHQKVVEIAPAPKLDPKIRQKMVECATHLARHVGYENAGTVEFLLDDKGNFYFIEVNARLQVEHTITEEVTGIDLVQSQIRIAEGMTLPELGLTQDKIQAQGYAIQCRVTTEDPANNFQPSTGRIEVFRSGEGMGIRLDSASTYAGAVISPYYDSLLVKVISHAHDLPSSAAKMNRALREFRIRGVKTNIPFLLNVLENQKFLNGSVDTYFIDENPQLFMFKASQNRAQKILNYLGYVLVNGPATPLATKIPPSDVKPYIPQVPLAVQPPKGYKQILDEGGPEAFAKAVRQHKGLLLMDTTFRDAHQSLLATRVRTHDLLAVSPYVAHNFNNLYSLENWGGATFDVALRFLHECPWERLHDMRQLIPNIPFQMLLRGANAVGYTNYPDNVVFKFCDMAVQAGMDIFRVFDSLNYLPNLVLGMEAAAKAGGVVEAAISYTGDVSDPSKTKYDLKYYTKLADDLVKAGTHVLAIKDMAGLLKPQAAKLLIGAIRAAHPHVPLHVHTHDTSGAGVAAMLACAEAGADVVDVAVDSVSGLTSQPSMGALVASLQGTPLDTKIPLQKVSEYSAYWEQARTLYGPFECTATMKSGNADVYLNEIPGGQYTNLQFQAFSLGLGSQFEEVKKAYREANLLLGDIIKVTPSSKVVGDLAQFMVQNKLTAADINARAEELSFPKSVVEYLQGAIGIPYGGFPEPLRSKVLKDMPRIEGRPGKELPSLNFDKLKGELKESFPDINDEDVMSAAMYPQVASDFFRFRDKYGPVKHLDTKTFLVGPAVGDTIEVKIERGKTLDIRTVAVSEDMTAAGEREVFFELNGQLRSVFIRDENASKEMKIHPKAVKGDKNQVGAPMPGTVLTIKVKEGDKVEKGQPIAVLSAMKMEMIVQAPKAGTVKTVSITNGQKLEGDDLICTIE

Description

Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.

Catalytic Activity

ATP + hydrogencarbonate + pyruvate = ADP + H(+) + oxaloacetate + phosphate

Cofactor

biotin

Uniprot

H9JFZ5   A0A212F7J4   A0A2A4K9L4   Q16V52   A0A084VZT4   A0A182G288   + More
Q7PMT9   A0A182NNB0   T1PNK0   Q16921   A0A182JB76   A0A1I8P066   A0A182P607   A0A034W4Y6   A0A0K8UXS3   A0A0K8USW6   A0A182WZW3   A0A182HXH2   A0A182R2R7   A0A182LI87   A7UUW7   A0A182MW77   T1PEF4   A0A182QSS0   A0A182YI47   B0W649   A0A182WAR7   A0A1I8P013   A0A1Q3FF39   A0A034W6F9   A0A0K8UP63   A0A1Q3FF69   A0A1Q3FEX2   A0A182TWU7   A0A0K8VFZ2   A0A1A9Z4H1   A0A1A9XPM5   A0A1A9UHC7   A0A1B0C5Z1   A0A087ZNH6   U5EW27   A0A2M4A2A8   A0A2M4BAS8   A0A2M4CTA7   A0A2M4CT64   A0A2M3YZ34   A0A1A9WWP3   A0A1L8DZ66   A0A0Q9WPE9   A0A2M4A2K2   A0A1W4UYU7   A0A195FNV1   A0A0Q5W9Y5   A0A1J1I8R4   A0A1L8DZ31   A0A2M4A2E8   A0A182FLU4   A0A0J9R931   A0A0R1DMG5   A0A0P8YBT1   Q7KN97   K7JBS6   A0A2M4CTK9   E2AWL4   N6V934   A0A232ERT7   A0A195DEG4   B4MKA9   A0A1W4ULX3   B3N6U9   A0A1J1ICE1   A0A026WMM7   A0A1B6DKU0   B4INM2   A0A0J9R952   A0A151XFU1   B3MGP4   Q0E9E2   B4NWM0   A0A3B0J5G8   B4H585   Q28XA7   A0A195BJA7   A0A1B0CVI4   A0A195CUW6   A0A3B0J1E1   E2BVP9   A0A0Q9W3Y3   A0A023F5Q0   A0A0Q9X9X3   A0A158NQ65   A0A2A3E744   A0A0C9QPW7   J9JSI7   A0A0T6BBM3   T1HWN2   B4LNV0   A0A224XGV8   A0A139WJ24   A0A034W1D1  

EC Number

6.4.1.1

Pubmed

19121390    22118469    17510324    24438588    26483478    12364791    + More
14747013    17210077    25315136    9066123    25348373    20966253    25244985    17994087    22936249    17550304    10731132    12537568    12537572    12537573    12537574    16110336    17569856    17569867    26109357    26109356    20075255    20798317    15632085    28648823    24508170    30249741    18057021    23185243    25474469    21347285    18362917    19820115   

EMBL

BABH01001672    BABH01001673    AGBW02009869    OWR49688.1    NWSH01000022    PCG80656.1    + More
CH477603    EAT38418.1    ATLV01018957    ATLV01018958    KE525256    KFB43478.1    JXUM01039418    KQ561203    KXJ79368.1    AAAB01008968    EAA13216.3    KA649508    AFP64137.1    L36530    AAB64306.1    GAKP01009588    GAKP01009585    GAKP01009583    JAC49367.1    GDHF01021169    JAI31145.1    GDHF01022701    JAI29613.1    APCN01005304    EDO63513.1    AXCM01000074    KA646500    AFP61129.1    AXCN02001904    DS231847    EDS36396.1    GFDL01008894    JAV26151.1    GAKP01009587    GAKP01009584    JAC49368.1    GDHF01023825    JAI28489.1    GFDL01008883    JAV26162.1    GFDL01008956    JAV26089.1    GDHF01014508    JAI37806.1    JXJN01026353    JXJN01026354    GANO01001610    JAB58261.1    GGFK01001625    MBW34946.1    GGFJ01000950    MBW50091.1    GGFL01004331    MBW68509.1    GGFL01004332    MBW68510.1    GGFM01000750    MBW21501.1    GFDF01002365    JAV11719.1    CH963846    KRF97599.1    GGFK01001619    MBW34940.1    KQ981382    KYN42091.1    CH954177    KQS70326.1    CVRI01000043    CRK96106.1    GFDF01002366    JAV11718.1    GGFK01001604    MBW34925.1    CM002911    KMY92593.1    CM000157    KRJ98535.1    CH902619    KPU76449.1    AF132152    AE013599    AAD34740.1    AAF58885.1    AAZX01015613    AAZX01016383    GGFL01004333    MBW68511.1    GL443346    EFN62178.1    CM000071    ENO01921.1    NNAY01002557    OXU21052.1    KQ980934    KYN11295.1    EDW72548.1    EDV58198.1    CRK96105.1    KK107154    QOIP01000001    EZA56916.1    RLU26312.1    GEDC01010999    JAS26299.1    CH676472    EDW49405.1    KMY92592.1    KQ982182    KYQ59246.1    EDV36802.1    KPU76448.1    AAG22289.2    EDW89565.1    OUUW01000001    SPP74993.1    CH479210    EDW32921.1    EAL26409.1    KRT03085.1    KQ976464    KYM84477.1    AJWK01030606    KQ977279    KYN04312.1    SPP74994.1    GL450924    EFN80232.1    CH940648    KRF79786.1    GBBI01002213    JAC16499.1    CH933808    KRG04949.1    ADTU01022847    ADTU01022848    KZ288358    PBC27046.1    GBYB01005689    JAG75456.1    ABLF02015508    ABLF02015509    ABLF02015512    ABLF02015513    ABLF02015516    ABLF02036200    ABLF02036209    ABLF02036211    ABLF02036212    ABLF02036215    LJIG01002196    KRT84744.1    ACPB03018676    EDW61119.1    KRF79787.1    GFTR01008614    JAW07812.1    KQ971338    KYB27787.1    GAKP01009586    JAC49366.1   

Proteomes

UP000005204    UP000007151    UP000218220    UP000008820    UP000030765    UP000069940    + More
UP000249989    UP000007062    UP000075884    UP000095301    UP000075880    UP000095300    UP000075885    UP000076407    UP000075840    UP000075900    UP000075882    UP000075883    UP000075886    UP000076408    UP000002320    UP000075920    UP000075902    UP000092445    UP000092443    UP000078200    UP000092460    UP000005203    UP000091820    UP000007798    UP000192221    UP000078541    UP000008711    UP000183832    UP000069272    UP000002282    UP000007801    UP000000803    UP000002358    UP000000311    UP000001819    UP000215335    UP000078492    UP000053097    UP000279307    UP000001292    UP000075809    UP000268350    UP000008744    UP000078540    UP000092461    UP000078542    UP000008237    UP000008792    UP000009192    UP000005205    UP000242457    UP000007819    UP000015103    UP000007266   

PRIDE

H9JFZ5    Q7KN97    Q0E9E2   

Pfam

PF00364   Biotin_lipoyl        + More
PF00289   Biotin_carb_N
PF00682   HMGL-like
PF02786   CPSase_L_D2
PF02785   Biotin_carb_C
PF02436   PYC_OADA

Interpro

IPR011764   Biotin_carboxylation_dom        + More
IPR003379   Carboxylase_cons_dom       
IPR005481   BC-like_N       
IPR005482   Biotin_COase_C       
IPR005930   Pyruv_COase       
IPR016185   PreATP-grasp_dom_sf       
IPR013785   Aldolase_TIM       
IPR011053   Single_hybrid_motif       
IPR000891   PYR_CT       
IPR011054   Rudment_hybrid_motif       
IPR001882   Biotin_BS       
IPR011761   ATP-grasp       
IPR005479   CbamoylP_synth_lsu-like_ATP-bd       
IPR000089   Biotin_lipoyl       

SUPFAM

SSF51230   SSF51230        + More
SSF52440   SSF52440       
SSF51246   SSF51246       

Gene 3D

3.20.20.70    

ProteinModelPortal

H9JFZ5   A0A212F7J4   A0A2A4K9L4   Q16V52   A0A084VZT4   A0A182G288   + More
Q7PMT9   A0A182NNB0   T1PNK0   Q16921   A0A182JB76   A0A1I8P066   A0A182P607   A0A034W4Y6   A0A0K8UXS3   A0A0K8USW6   A0A182WZW3   A0A182HXH2   A0A182R2R7   A0A182LI87   A7UUW7   A0A182MW77   T1PEF4   A0A182QSS0   A0A182YI47   B0W649   A0A182WAR7   A0A1I8P013   A0A1Q3FF39   A0A034W6F9   A0A0K8UP63   A0A1Q3FF69   A0A1Q3FEX2   A0A182TWU7   A0A0K8VFZ2   A0A1A9Z4H1   A0A1A9XPM5   A0A1A9UHC7   A0A1B0C5Z1   A0A087ZNH6   U5EW27   A0A2M4A2A8   A0A2M4BAS8   A0A2M4CTA7   A0A2M4CT64   A0A2M3YZ34   A0A1A9WWP3   A0A1L8DZ66   A0A0Q9WPE9   A0A2M4A2K2   A0A1W4UYU7   A0A195FNV1   A0A0Q5W9Y5   A0A1J1I8R4   A0A1L8DZ31   A0A2M4A2E8   A0A182FLU4   A0A0J9R931   A0A0R1DMG5   A0A0P8YBT1   Q7KN97   K7JBS6   A0A2M4CTK9   E2AWL4   N6V934   A0A232ERT7   A0A195DEG4   B4MKA9   A0A1W4ULX3   B3N6U9   A0A1J1ICE1   A0A026WMM7   A0A1B6DKU0   B4INM2   A0A0J9R952   A0A151XFU1   B3MGP4   Q0E9E2   B4NWM0   A0A3B0J5G8   B4H585   Q28XA7   A0A195BJA7   A0A1B0CVI4   A0A195CUW6   A0A3B0J1E1   E2BVP9   A0A0Q9W3Y3   A0A023F5Q0   A0A0Q9X9X3   A0A158NQ65   A0A2A3E744   A0A0C9QPW7   J9JSI7   A0A0T6BBM3   T1HWN2   B4LNV0   A0A224XGV8   A0A139WJ24   A0A034W1D1  

PDB

3BG5     E-value=0,     Score=2710

KEGG

101737640    K01958 pyruvate carboxylase [EC:6.4.1.1] | (RefSeq) pyruvate carboxylase, mitochondrial

PATHWAY

00020    Citrate cycle (TCA cycle) - Bombyx mori (domestic silkworm)
00620    Pyruvate metabolism - Bombyx mori (domestic silkworm)
01100    Metabolic pathways - Bombyx mori (domestic silkworm)
01200    Carbon metabolism - Bombyx mori (domestic silkworm)
01230    Biosynthesis of amino acids - Bombyx mori (domestic silkworm)

GO

GO:0006090   GO:0009374   GO:0046872   GO:0006094   GO:0004736   GO:0005524   GO:0005739   GO:0005759   GO:0005737   GO:0003824   GO:0003677   GO:0004075  

Length:

1182

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.00245

Exp number, first 60 AAs:

0.0002

Total prob of N-in:

0.00011

outside

1  -  1182

 

 

Pi

230.831632

Theta

167.394498

Tajima's D

0.94483

CLR

0.757147

CSRT

0.641217939103045

Interpretation

Uncertain