Gene

KWMTBOMO10296  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA007265

Annotation

PREDICTED:_translation_factor_GUF1_homolog?_mitochondrial_isoform_X1_[Papilio_machaon]

Location

Bomo_Chr17(-):7128694-7137214      View in EpiBrowser

Full name

Translation factor GUF1 homolog, mitochondrial       + More
Translation factor waclaw, mitochondrial      
Translation factor GUF1, mitochondrial      

Alternative Name

Elongation factor 4 homolog
GTPase GUF1 homolog
Ribosomal back-translocase
GTPase GUF1

Location in the cell

Cytoplasmic   Reliability : 1.838 Mitochondrial   Reliability : 1.474

CDS

ATGGTTACTAATATAAATTTTATAAAATACTTGTATTACAATTCTGTTAAATATCATCAAAGGGTCTTAATGAGACGTATGAGTTCAGACTCTAGGAAATATGTAGATGTGGAAAAAATAAGGAACTTTAGCATTGTAGCTCACGTCGATCATGGCAAGAGCACTTTGGCTGACAGACTTCTTGAGTTAACCGGTGTAAAGAAACCGGGCGATGAACAATCTCAAGTTTTGGACCAACTACAGGTTGAAAAAGAAAGAGGAATTACAGTGAAGGCAGTCACGGCTACTCTAGACTACACATATAACAATGAGACGTACCTACTCAATTTAATTGACACTCCTGGACATGTGGATTTCTCCAATGAAGTAGTGAGAAGTGTAACAGCATGTCAAGGAGTCATTATGCTGGTGGATGCTAATGAAGGTATTCAAGCACAAACAGTAGCTGTTCATTCATTAGCTAAGAGAAACAATTTAGTAATAATACCAACACTAAACAAAGTGGATCTTCCAAGAGCTAACCCTGACAAAGTGAAAGAACAGTTACAATCAGTATTCAAAATAGACCCTAATACAGTTTTAAGCATATCAGCAAAAAAAGGTTGGGGTGTGCGAGAGCTACTAGAAGCTGTAATAATGAGGATCCCACCACCAAAAGTTGTCTTAGATGATCATTTCCGTGCTCACATAATAGACACATGGCATGATAAATATAGAGGGGTTTTGTGTTTGGCATATATTACTTCAGGGACAGTTAAAGTTGGACAAGCAGTGAAATGGCAATCTGATTCGAAATTACAAACAGTGAAGCACTTGGCACTGTTGAGACCTACTGAGGAGCCCATTGATGAAGCTATTGCAGGTCAAGTTGTAATGGTAGGTTGTGGACCAAAAGGCGGTGGTTCTGTTGGGGATCAATTAGTAGCTAAAGAATTAGCTAATGCTAGTGAAAGAATATCTGCACCTGCAGTGAAGCACATGGTCTACGCTGGTATATTTCCTGCAGACCAATCACAGCATATATTACTGAGTGATGCAATCAAGAAATTAGCTTTAAATGATTCGGCTGTTTCAGTTACGATAGATTCAAGTCCAGCTCTAGGTCAAGGATGGCGTATTGGATTTCTGGGCTTGTTGCACTTAGATGTTTTTACACAACGTTTATTGCAAGAACACAAAGCCGAGGCAATTCTCACAGCACCTTCAGTGCCCTATAAAGTTAAAATAAAAGGAGCCAAACTTATAAAACATTACAAGACTGATGAAATTTTAATCACAAATCCTTTACAATTACCGGACCCAAACAATGTAGTAGAATATTTCGAACCATTTGTTAAAGGTACAATAATTACACCTGTAGAATACATAGGCGCGGTGACATCCCTGTGCATAGACAGACGGGGCACACCCCTACCGTCCAGCGCAGTCGACGACACGATGACCATGTTACAGTTTATCCTACCGCTATCCGAAATCGTCGTCGATTTTCACGATACATTAAAAAGCATAACTTCAGGATTCGCTAGCTTTGATTATGAAGATGACGGCTTTCACTCCAGCTCTTTGGTCCGAATGGATATTTTAATCAATGGTATGCTTGTTGATGAATTATCAACAATAGTGCACGCTAGTCGACTAGAGTATAATGCGCGGAGATTAGCTGAGAAATTAAAAGAAATGATCCCTAGGCAAATGGTGCAGGTTGCCATACAAGCAGTAGTGGGGGGAAAAGTATTCGCACGAGAAACCATCAAAGCCTACAGAAAAGACGTCACCGCTAAATTGTACGGCGGCGACGTTACTCGAAGAAAGAAATTATTAAAGCAACAAGCCGAGGGTAAGAAAAAAATGCGCAGCGTAGCGAACATCAAAATTCCGAGAGATACGTTTATAGACGTGTTGAAAAAGTAG

Protein

MVTNINFIKYLYYNSVKYHQRVLMRRMSSDSRKYVDVEKIRNFSIVAHVDHGKSTLADRLLELTGVKKPGDEQSQVLDQLQVEKERGITVKAVTATLDYTYNNETYLLNLIDTPGHVDFSNEVVRSVTACQGVIMLVDANEGIQAQTVAVHSLAKRNNLVIIPTLNKVDLPRANPDKVKEQLQSVFKIDPNTVLSISAKKGWGVRELLEAVIMRIPPPKVVLDDHFRAHIIDTWHDKYRGVLCLAYITSGTVKVGQAVKWQSDSKLQTVKHLALLRPTEEPIDEAIAGQVVMVGCGPKGGGSVGDQLVAKELANASERISAPAVKHMVYAGIFPADQSQHILLSDAIKKLALNDSAVSVTIDSSPALGQGWRIGFLGLLHLDVFTQRLLQEHKAEAILTAPSVPYKVKIKGAKLIKHYKTDEILITNPLQLPDPNNVVEYFEPFVKGTIITPVEYIGAVTSLCIDRRGTPLPSSAVDDTMTMLQFILPLSEIVVDFHDTLKSITSGFASFDYEDDGFHSSSLVRMDILINGMLVDELSTIVHASRLEYNARRLAEKLKEMIPRQMVQVAIQAVVGGKVFARETIKAYRKDVTAKLYGGDVTRRKKLLKQQAEGKKKMRSVANIKIPRDTFIDVLKK

Description

Promotes mitochondrial protein synthesis. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Binds to mitochondrial ribosomes in a GTP-dependent manner.

Catalytic Activity

GTP + H2O = GDP + H(+) + phosphate

Miscellaneous

This protein may be expected to contain an N-terminal transit peptide but none has been predicted.

Similarity

Belongs to the GTP-binding elongation factor family. LepA subfamily.
Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. LepA subfamily.

Keywords

Complete proteome   GTP-binding   Hydrolase   Membrane   Mitochondrion   Mitochondrion inner membrane   Nucleotide-binding   Protein biosynthesis   Reference proteome   Transit peptide  

Feature

chain  Translation factor GUF1 homolog, mitochondrial

Uniprot

H9JCM0   A0A2H1W0S4   A0A2A4JLQ2   A0A3S2P9K5   A0A194QT37   A0A194QCR0   + More
A0A212FDE4   B0WXB8   A0A1Q3FCB3   A0A1Y1L517   Q0IFX5   W5JUN6   A0A182GJD8   A0A182T5N1   A0A182XGW6   A0A182RWS6   A0A182KZ19   A0A182PCG3   A0A182KGD9   Q7PMF6   A0A182W8T0   A0A1S4H2L6   B4JNG0   A0A182M5S3   A0A182TJP4   A0A182IZJ7   A0A182HRB1   A0A0M4F967   A0A182UQQ6   A0A182F0T2   B4NCH5   B4L5D7   A0A182NP40   A0A0A1X703   X4YDX6   Q9VRH6   Q86P01   A0A0K8VQR5   A0A182Q138   A0A1I8MJZ7   B4MAZ7   B5DKL9   B4R3R6   B4HD31   B3NYD8   B3MRW0   B4I6H2   B4PZ34   A0A1B0FCK3   A0A1A9YNV1   A0A3B0JCJ5   A0A1B0AEB4   A0A1I8PR26   A0A1B0APL7   W8BL04   A0A182Y6B8   A0A1A9X1G1   A0A1J1I7Z7   A0A1A9UL95   U4UHZ1   A0A026VTH6   A0A034WD04   A0A1B0DJF5   N6TUM5   A0A195FYN7   A0A0P4VQT4   T1HN81   N6TX65   A0A0N8BER3   E9GF27   A0A0C9RII1   A0A0P5QE36   A0A069DVY7   A0A151IYU8   A0A0P4XS62   A0A0P6GQK2   A0A023F4I2   A0A0P5J3B2   A0A0J7NRZ9   E2A9H9   A0A0P4ZJX9   E9JD47   E2BW13   A0A0P5RFK9   A0A0P4WS90   A0A151I492   A0A158NXT4   A0A0N8ABL3   A0A0P5XBV6   H3AGD0   A0A0P6AZP7   F4WLU1   A0A164RWH3   I3KFH1   A0A1S3RR63   A0A060XKC1   H3AGD1   A0A3P9BCX5   A0A310SDA6   A0A2D0RF47  

EC Number

3.6.5.n1
3.6.5.-

Pubmed

19121390    26354079    22118469    28004739    17510324    20920257    + More
23761445    26483478    20966253    12364791    17994087    25830018    9520435    10731132    12537572    25315136    15632085    17550304    24495485    25244985    23537049    24508170    30249741    25348373    27129103    21292972    26334808    25474469    20798317    21282665    21347285    9215903    21719571    25186727    24755649   

EMBL

BABH01034693    ODYU01005610    SOQ46627.1    NWSH01001074    PCG72749.1    RSAL01000168    + More
RVE45234.1    KQ461155    KPJ08165.1    KQ459232    KPJ02770.1    AGBW02009056    OWR51785.1    DS232161    GFDL01009848    JAV25197.1    GEZM01068259    JAV67065.1    CH477286    ADMH02000444    ETN66459.1    JXUM01067886    KQ562472    KXJ75822.1    AAAB01008980    EAA13916.4    CH916371    EDV92253.1    AXCM01000552    APCN01001664    CP012528    ALC49037.1    CH964239    EDW82534.1    CH933811    EDW06396.1    GBXI01009457    GBXI01007405    JAD04835.1    JAD06887.1    BT150439    AHV85501.1    AF017777    AE014298    BT003552    AAO39556.1    GDHF01026205    GDHF01023022    GDHF01020170    GDHF01011116    GDHF01010385    GDHF01001301    JAI26109.1    JAI29292.1    JAI32144.1    JAI41198.1    JAI41929.1    JAI51013.1    AXCN02001967    CH940655    EDW66406.2    CH379063    EDY72069.2    CM000366    EDX18534.1    CH479636    EDW35356.1    CH954180    EDV47617.2    CH902622    EDV34515.2    CH480823    EDW56378.1    CM000162    EDX03095.2    CCAG010004527    CCAG010004528    OUUW01000003    SPP77812.1    JXJN01001483    GAMC01012594    GAMC01012590    JAB93961.1    CVRI01000043    CRK96439.1    KB632308    ERL91963.1    KK107941    QOIP01000004    EZA47093.1    RLU23968.1    GAKP01006927    JAC52025.1    AJVK01015784    APGK01051610    KB741197    ENN72980.1    KQ981204    KYN44944.1    GDKW01001741    JAI54854.1    ACPB03015116    ENN72981.1    GDIQ01184955    JAK66770.1    GL732541    EFX81974.1    GBYB01008055    JAG77822.1    GDIQ01136766    JAL14960.1    GBGD01000893    JAC87996.1    KQ980761    KYN13602.1    GDIP01238823    JAI84578.1    GDIQ01042559    JAN52178.1    GBBI01002620    JAC16092.1    GDIQ01205261    JAK46464.1    LBMM01002135    KMQ95220.1    GL437912    EFN69903.1    GDIP01211684    JAJ11718.1    GL771866    EFZ09158.1    GL451091    EFN80045.1    GDIQ01101444    JAL50282.1    GDRN01066268    GDRN01066267    GDRN01066266    JAI64571.1    KQ976462    KYM84642.1    ADTU01003520    ADTU01003521    GDIP01163433    JAJ59969.1    GDIP01074452    JAM29263.1    AFYH01140933    AFYH01140934    AFYH01140935    AFYH01140936    GDIP01023074    JAM80641.1    GL888216    EGI64823.1    LRGB01002121    KZS09009.1    AERX01026919    AERX01026920    FR905222    CDQ77330.1    KQ761108    OAD58203.1   

Proteomes

UP000005204    UP000218220    UP000283053    UP000053240    UP000053268    UP000007151    + More
UP000002320    UP000008820    UP000000673    UP000069940    UP000249989    UP000075901    UP000076407    UP000075900    UP000075882    UP000075885    UP000075881    UP000007062    UP000075920    UP000001070    UP000075883    UP000075902    UP000075880    UP000075840    UP000092553    UP000075903    UP000069272    UP000007798    UP000009192    UP000075884    UP000000803    UP000075886    UP000095301    UP000008792    UP000001819    UP000000304    UP000008744    UP000008711    UP000007801    UP000001292    UP000002282    UP000092444    UP000092443    UP000268350    UP000092445    UP000095300    UP000092460    UP000076408    UP000091820    UP000183832    UP000078200    UP000030742    UP000053097    UP000279307    UP000092462    UP000019118    UP000078541    UP000015103    UP000000305    UP000078492    UP000036403    UP000000311    UP000008237    UP000078540    UP000005205    UP000008672    UP000007755    UP000076858    UP000005207    UP000087266    UP000193380    UP000265160    UP000221080   

PRIDE

H9JCM0    B0WXB8    Q0IFX5    X4YDX6    Q9VRH6    Q86P01    + More

Pfam

PF00009   GTP_EFTU        + More
PF00679   EFG_C
PF06421   LepA_C
PF00060   Lig_chan
PF09767   DUF2053
PF03144   GTP_EFTU_D2

Interpro

IPR009000   Transl_B-barrel_sf        + More
IPR006297   EF-4       
IPR035647   EFG_III/V       
IPR027417   P-loop_NTPase       
IPR013842   LepA_CTD       
IPR035654   LepA_IV       
IPR000795   TF_GTP-bd_dom       
IPR038363   LepA_C_sf       
IPR000640   EFG_V-like       
IPR005225   Small_GTP-bd_dom       
IPR031157   G_TR_CS       
IPR019164   TMEM147       
IPR001320   Iontro_rcpt       
IPR004161   EFTu-like_2       

SUPFAM

SSF52540   SSF52540        + More
SSF54980   SSF54980       
SSF50447   SSF50447       

Gene 3D

3.30.70.2570    

CDD

cd03709  lepA_C

ProteinModelPortal

H9JCM0   A0A2H1W0S4   A0A2A4JLQ2   A0A3S2P9K5   A0A194QT37   A0A194QCR0   + More
A0A212FDE4   B0WXB8   A0A1Q3FCB3   A0A1Y1L517   Q0IFX5   W5JUN6   A0A182GJD8   A0A182T5N1   A0A182XGW6   A0A182RWS6   A0A182KZ19   A0A182PCG3   A0A182KGD9   Q7PMF6   A0A182W8T0   A0A1S4H2L6   B4JNG0   A0A182M5S3   A0A182TJP4   A0A182IZJ7   A0A182HRB1   A0A0M4F967   A0A182UQQ6   A0A182F0T2   B4NCH5   B4L5D7   A0A182NP40   A0A0A1X703   X4YDX6   Q9VRH6   Q86P01   A0A0K8VQR5   A0A182Q138   A0A1I8MJZ7   B4MAZ7   B5DKL9   B4R3R6   B4HD31   B3NYD8   B3MRW0   B4I6H2   B4PZ34   A0A1B0FCK3   A0A1A9YNV1   A0A3B0JCJ5   A0A1B0AEB4   A0A1I8PR26   A0A1B0APL7   W8BL04   A0A182Y6B8   A0A1A9X1G1   A0A1J1I7Z7   A0A1A9UL95   U4UHZ1   A0A026VTH6   A0A034WD04   A0A1B0DJF5   N6TUM5   A0A195FYN7   A0A0P4VQT4   T1HN81   N6TX65   A0A0N8BER3   E9GF27   A0A0C9RII1   A0A0P5QE36   A0A069DVY7   A0A151IYU8   A0A0P4XS62   A0A0P6GQK2   A0A023F4I2   A0A0P5J3B2   A0A0J7NRZ9   E2A9H9   A0A0P4ZJX9   E9JD47   E2BW13   A0A0P5RFK9   A0A0P4WS90   A0A151I492   A0A158NXT4   A0A0N8ABL3   A0A0P5XBV6   H3AGD0   A0A0P6AZP7   F4WLU1   A0A164RWH3   I3KFH1   A0A1S3RR63   A0A060XKC1   H3AGD1   A0A3P9BCX5   A0A310SDA6   A0A2D0RF47  

PDB

2YWH     E-value=1.50925e-143,     Score=1308

KEGG

101735786    K21594 translation factor GUF1, mitochondrial [EC:3.6.5.-] | (RefSeq) translation factor GUF1 homolo

GO

GO:0043022   GO:0045727   GO:0003924   GO:0006412   GO:0005743   GO:0005759   GO:0005525   GO:0005739   GO:0016021   GO:0004970   GO:0046923   GO:0015935   GO:0004812   GO:0006418   GO:0003723   GO:0046872   GO:0003824  

Subcellular location

Mitochondrion inner membrane  

Length:

636

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.07931

Exp number, first 60 AAs:

0.00185

Total prob of N-in:

0.00155

outside

1  -  636

 

 

Pi

228.115229

Theta

165.22659

Tajima's D

0.988699

CLR

0.261872

CSRT

0.653917304134793

Interpretation

Uncertain