Gene

KWMTBOMO10291

Annotation

DNA_polymerase_accessory_subunit_[Bombyx_mori]

Location

Bomo_Chr17(+):7100125-7105735      View in EpiBrowser

Full name

Glutamyl-tRNA(Gln) amidotransferase subunit C, mitochondrial       + More
DNA polymerase subunit gamma-2, mitochondrial      

Alternative Name

Mitochondrial DNA polymerase accessory subunit
MtPolB
PolG-beta

Location in the cell

Mitochondrial   Reliability : 1.174 Nuclear   Reliability : 1.766

CDS

ATGAAAACTGAAATTCTTAAAGTTTTAAACCTGAAAAAATTCTTTCAAATGATTAATAATAATGCAACAAAAATTGATTTTACTTTATTGAAACCTAGTCAGGTATTAATGAAAAATATCCATGTGAATTGGCTCCGGTTCACATTAGGCAAAACACAGAAACACATGCCAGTTTATTTGAATAGAAAAACATTTGAGTTATGTGATAAGAATATACCATTTGGTTTTATAACAATTGATAAACAATCTACCGAAATACTAACTCACAACCTGAGAAGTGTTTATCCAGAAGAAACTTCTAAATTAGCACTAAACATAATTATACCCCAAAAAGACATAATGAAAATTTTTATTCAATGGCAACGATATAGGAAATACTGGTGGAGTTCTATAACCACAACACCCAGCTTGTTTAGCATAAATGACATGAAGTTGGAAAATAATGAAGCCAATGTTGATATATTAGCAAAATTTCCTTTTGGATCATTTCCTGTTGAAAGAATTACTATTACATGCGATGATAATGAAAGTAACAATTATCGCCTCAGTTGTACCATGTCATTAGAAAATGCACTATTTATAATACTCCTTGATGGGTTAAGTAATTACTCAAAAGAAGAATATTTGAGACTTCACCGAAAAATCACACCATATAAGATTGCCTTTGGTTTCAATTTTGAAGATAACAAAAATAAGGAACGATTGAGTAAACTCGTTCAAGTATTGTTCAACAAATTGGAGGGAAACCAAATTGCTGCATTGTTACCCTCAATCCCAATGAGTCTAGATTCTCAGATCAAAGAAAATCTTCAAATGGGCGTAACCTATACTGCTATCGTCGATGAAACAACCTTAGAAAATGGTATATTCCAATTACTGAACAGCAGCACAATGTTGAAGGAACAAGTTCACGTTGCTGACTTCCATGAATATGCGTCAAGTTTATTCAACTACTAA

Protein

MKTEILKVLNLKKFFQMINNNATKIDFTLLKPSQVLMKNIHVNWLRFTLGKTQKHMPVYLNRKTFELCDKNIPFGFITIDKQSTEILTHNLRSVYPEETSKLALNIIIPQKDIMKIFIQWQRYRKYWWSSITTTPSLFSINDMKLENNEANVDILAKFPFGSFPVERITITCDDNESNNYRLSCTMSLENALFIILLDGLSNYSKEEYLRLHRKITPYKIAFGFNFEDNKNKERLSKLVQVLFNKLEGNQIAALLPSIPMSLDSQIKENLQMGVTYTAIVDETTLENGIFQLLNSSTMLKEQVHVADFHEYASSLFNY

Description

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).
Mitochondrial polymerase processivity subunit. Stimulates the polymerase and exonuclease activities, and increases the processivity of the enzyme. Binds to ss-DNA (By similarity).

Catalytic Activity

ATP + H2O + L-glutamine + L-glutamyl-tRNA(Gln) = ADP + H(+) + L-glutamate + L-glutaminyl-tRNA(Gln) + phosphate
a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1)

Subunit

Subunit of the heterotrimeric GatCAB amidotransferase (AdT) complex, composed of A, B and C subunits.
Heterotrimer composed of a catalytic subunit and a homodimer of accessory subunits.

Similarity

Belongs to the GatC family.

Keywords

Direct protein sequencing   DNA replication   DNA-binding   DNA-directed DNA polymerase   Mitochondrion   Nucleotidyltransferase   Transferase   Transit peptide  

Feature

chain  DNA polymerase subunit gamma-2, mitochondrial

Uniprot

Q2F675   A0A2H1W0U7   A0A2A4JMW4   A0A3S2NEA5   A0A212FDL1   A0A1Y1NBC5   + More
A0A139WDR4   A0A2H8TIA5   B7PY83   A0A0P4VSM6   R4G8F3   A0A1B6DQ67   A0A131XXJ1   N6SUA8   A0A1W4WIE1   J9K8P9   A0A2J7R6A9   A0A2S2NRR2   A0A182SDB3   A0A1B6LUF5   A0A0L7QRI6   E2AC12   A0A182YK52   A0A1B6K230   A0A088A9B7   A0A195FEE8   E2BXH0   A0A0J7L7V2   A0A3Q0JI74   F4WPW1   A0A0A9Y702   A0A151WRA3   A0A195EG40   A0A182J9N4   A0A195CKT4   A0A182PPW3   A0A182V6H7   A0A147BP98   A0A2A3EFC9   A0A182UBM4   A0A3F2Z1W2   A0A158NHY6   A0A195AWB4   U5EQI8   A0A0C9RMJ2   U6D8E4   R0K8U3   U3INA9   A0A0K8TSI6   R7VWR2   A0A099ZZN2   A0A182MAF8   A0A2K6GFA6   Q4RLF8   Q5TWB1   A0A3L8DA34   A0A093P3L8   A0A026WXB8   A0A087V4Y9   A0A093PKY5   A0A3Q2ZLM8   A0A091GB79   A0A1V4KVU4   B7ZRE8   Q9W6G7   A0A091IZ60   A0A182RCF6   A0A1S3GY49   A0A182W545   A0A093HWV4   A0A087RIA8   A0A182IDP9   W4ZHE9   F6VZT2   A0A093GPR1   A0A3P4KE08   A0A2Y9L284   A0A2I0M747   A0A091WDZ2   H3B5K7   H9G4Q9   A0A091V8J6   A0A1W4VXT9   A0A091LW17   A0A3B3ZLC2   H0Z2L8   F1NM24   M3YTH8   H3C366   F7EFE8   A0A1J1IQ12   A0A091L4V3  

EC Number

6.3.5.-
2.7.7.7

Pubmed

22118469    28004739    18362917    19820115    27129103    23537049    + More
20798317    25244985    21719571    25401762    26823975    29652888    21347285    23749191    26369729    23371554    15496914    12364791    14747013    17210077    30249741    24508170    27762356    10330144    20431018    9215903    21881562    25463417    20360741    15592404    18464734   

EMBL

DQ311197    ABD36142.1    ODYU01005610    SOQ46633.1    NWSH01001074    PCG72740.1    + More
RSAL01000168    RVE45239.1    AGBW02009056    OWR51787.1    GEZM01007320    JAV95242.1    KQ971358    KYB25981.1    GFXV01001945    MBW13750.1    ABJB010238919    ABJB010580419    DS818309    EEC11555.1    GDKW01000457    JAI56138.1    ACPB03015962    GAHY01001072    JAA76438.1    GEDC01009545    JAS27753.1    GEFM01005390    JAP70406.1    APGK01057078    KB741277    KB631617    ENN71284.1    ERL84696.1    ABLF02005752    ABLF02005754    NEVH01006828    PNF36375.1    GGMR01007149    MBY19768.1    GEBQ01012686    JAT27291.1    KQ414777    KOC61237.1    GL438389    EFN69030.1    GECU01035072    GECU01002199    JAS72634.1    JAT05508.1    KQ981636    KYN38778.1    GL451254    EFN79618.1    LBMM01000249    KMR03015.1    GL888255    EGI63790.1    GBHO01018294    GBRD01006368    GDHC01007407    JAG25310.1    JAG59453.1    JAQ11222.1    KQ982813    KYQ50331.1    KQ978957    KYN27203.1    KQ977622    KYN01318.1    GEGO01003239    JAR92165.1    KZ288259    PBC30493.1    ADTU01016112    KQ976725    KYM76538.1    GANO01004226    JAB55645.1    GBYB01009560    JAG79327.1    HAAF01007052    CCP78876.1    KB742588    EOB06681.1    ADON01023570    GDAI01000271    JAI17332.1    KB375583    EMC84364.1    KL870339    KGL87779.1    AXCM01000617    CAAE01015021    CAG10774.1    AAAB01008811    EAL41674.4    QOIP01000011    RLU17196.1    KL225043    KFW66857.1    KK107078    EZA60391.1    KL480131    KFO07681.1    KL668963    KFW74935.1    KL447899    KFO78404.1    LSYS01001520    OPJ88536.1    BC170142    BC170144    CM004482    AAI70142.1    OCT63175.1    AF124606    KK501252    KFP13647.1    KL206555    KFV83442.1    KL226380    KFM13212.1    APCN01004765    AAGJ04105563    AAMC01124950    AAMC01124951    KL216287    KFV69017.1    CYRY02000492    VCW50019.1    AKCR02000032    PKK25504.1    KK735345    KFR13784.1    AFYH01051037    AFYH01051038    AFYH01051039    AFYH01051040    AAWZ02021918    AAWZ02021919    KL410718    KFQ98717.1    KK511781    KFP63351.1    ABQF01037353    AADN05000628    AEYP01025757    AEYP01025758    CVRI01000057    CRL02311.1    KL298691    KFP50872.1   

Proteomes

UP000218220    UP000283053    UP000007151    UP000007266    UP000001555    UP000015103    + More
UP000019118    UP000030742    UP000192223    UP000007819    UP000235965    UP000075901    UP000053825    UP000000311    UP000076408    UP000005203    UP000078541    UP000008237    UP000036403    UP000079169    UP000007755    UP000075809    UP000078492    UP000075880    UP000078542    UP000075885    UP000075903    UP000242457    UP000075902    UP000076407    UP000005205    UP000078540    UP000016666    UP000053858    UP000075883    UP000233160    UP000007303    UP000007062    UP000279307    UP000054081    UP000053097    UP000053258    UP000264820    UP000053760    UP000190648    UP000186698    UP000053119    UP000075900    UP000081671    UP000075920    UP000053584    UP000053286    UP000075840    UP000007110    UP000008143    UP000053875    UP000248482    UP000053872    UP000053605    UP000008672    UP000001646    UP000053283    UP000192221    UP000261520    UP000007754    UP000000539    UP000000715    UP000002279    UP000183832   

PRIDE

Q2F675    F1NM24   

Pfam

PF03129   HGTP_anticodon        + More
PF02686   Glu-tRNAGln
PF00476   DNA_pol_A
PF18136   DNApol_Exo

Interpro

IPR027030   POLG2        + More
IPR027031   Gly-tRNA_synthase/POLG2       
IPR036621   Anticodon-bd_dom_sf       
IPR004154   Anticodon-bd       
IPR003837   Asp/Glu-ADT_csu       
IPR036113   Asp/Glu-ADT_sf_sub_c       
IPR042064   POLG2_C       
IPR033731   GlyRS-like_core       
IPR001098   DNA-dir_DNA_pol_A_palm_dom       
IPR012337   RNaseH-like_sf       
IPR002297   DNA-dir_DNA_pol_A_mt       
IPR041336   DNApol_Exo       
IPR019760   DNA-dir_DNA_pol_A_CS       

SUPFAM

SSF141000   SSF141000        + More
SSF53098   SSF53098       

Gene 3D

3.40.50.800     1.10.20.60    

CDD

cd02426  Pol_gamma_b_Cterm
cd00774  GlyRS-like_core

ProteinModelPortal

Q2F675   A0A2H1W0U7   A0A2A4JMW4   A0A3S2NEA5   A0A212FDL1   A0A1Y1NBC5   + More
A0A139WDR4   A0A2H8TIA5   B7PY83   A0A0P4VSM6   R4G8F3   A0A1B6DQ67   A0A131XXJ1   N6SUA8   A0A1W4WIE1   J9K8P9   A0A2J7R6A9   A0A2S2NRR2   A0A182SDB3   A0A1B6LUF5   A0A0L7QRI6   E2AC12   A0A182YK52   A0A1B6K230   A0A088A9B7   A0A195FEE8   E2BXH0   A0A0J7L7V2   A0A3Q0JI74   F4WPW1   A0A0A9Y702   A0A151WRA3   A0A195EG40   A0A182J9N4   A0A195CKT4   A0A182PPW3   A0A182V6H7   A0A147BP98   A0A2A3EFC9   A0A182UBM4   A0A3F2Z1W2   A0A158NHY6   A0A195AWB4   U5EQI8   A0A0C9RMJ2   U6D8E4   R0K8U3   U3INA9   A0A0K8TSI6   R7VWR2   A0A099ZZN2   A0A182MAF8   A0A2K6GFA6   Q4RLF8   Q5TWB1   A0A3L8DA34   A0A093P3L8   A0A026WXB8   A0A087V4Y9   A0A093PKY5   A0A3Q2ZLM8   A0A091GB79   A0A1V4KVU4   B7ZRE8   Q9W6G7   A0A091IZ60   A0A182RCF6   A0A1S3GY49   A0A182W545   A0A093HWV4   A0A087RIA8   A0A182IDP9   W4ZHE9   F6VZT2   A0A093GPR1   A0A3P4KE08   A0A2Y9L284   A0A2I0M747   A0A091WDZ2   H3B5K7   H9G4Q9   A0A091V8J6   A0A1W4VXT9   A0A091LW17   A0A3B3ZLC2   H0Z2L8   F1NM24   M3YTH8   H3C366   F7EFE8   A0A1J1IQ12   A0A091L4V3  

PDB

5C53     E-value=2.24423e-12,     Score=174

KEGG

692827    K02333 DNA polymerase gamma 2 | (RefSeq) DNA polymerase accessory subunit

GO

GO:0006260   GO:0005739   GO:0006281   GO:0032543   GO:0005524   GO:0050567   GO:0006450   GO:0030956   GO:0070681   GO:0006264   GO:0003887   GO:0005737   GO:0070584   GO:0032042   GO:0022904   GO:0042802   GO:0042645   GO:0001701   GO:0003677   GO:0005760   GO:0016021  

PANTHER

PTHR10745   PTHR10745:SF8   PTHR15004   PTHR10267  

Subcellular location

Mitochondrion  

Length:

318

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.0178

Exp number, first 60 AAs:

0.0013

Total prob of N-in:

0.02702

outside

1  -  318

 

 

Pi

140.317075

Theta

155.728878

Tajima's D

0.183354

CLR

0.133843

CSRT

0.421678916054197

Interpretation

Uncertain