Gene

KWMTBOMO09898

Pre Gene Modal

BGIBMGA013165

Annotation

PREDICTED:_cell_cycle_checkpoint_protein_RAD1_[Amyelois_transitella]

Location

Bomo_Chr16(+):11474974-11482973      View in EpiBrowser

Full name

Cell cycle checkpoint protein RAD1      

Alternative Name

DNA repair exonuclease rad1 homolog
Rad1-like DNA damage checkpoint protein

Location in the cell

Cytoplasmic   Reliability : 1.75 Extracellular   Reliability : 1.799

CDS

ATGGCTGAAGATTCATCTCAATACCATTTCACGGCTAGCATGGACTCCGGAAAGACTCTGTGTAGTTTATTAAAAGCAATACAGTTTCAAGAGTCAGCAGTGTTCTGTGCTCTACCTGAAGGTTTGAAGTTAACAGTGGAGGAAGGCAAGTGTGTTCAAGCCTCAGCTTATATACCGGCTGACAACTTCACAGAATACCATGTTAGGGATGATGTAGATGTCATATTTAAGATCAGCTTAGCAGTTTTAGCAGAATGCTTAAACATATTTGGCTCGGGTGAAGAGGCCAGCTTGAAAATGTATTACAAATGTGAAGGATCCCCGCTGTTACTTGTCTTACAGCCTCAGTCGTTTCGTAACGTCATGACGGACTGCGAGATATGGAGCCAGACCCCAGATTCGATGCTGGAGCTTCGCAGTCCCGAGGAGACGGACGTGGCGAAGCTCGTGGTCCGCGCCGGAGCCCTACTCGCAGTACTGGGGGACGTCGAGAGGAGTGCCGATGTCTTGGAGTTGAGGTTGCAGCCTGAACCGCCACACTTCACCATCGTTACGTACGGAATGCAGGATCGATCCTGTATCGAAGTGCCTGAGTGCTCGGACACACTGCAGAGCTTCAGCTGTGAACGAGAACTGACCCTCAAATATCAACTCCACCACCTCAGGCTCTGCATGAAAGCTCTAGCTATTTCAAATAAGGTCGTATTAAGATGCAGCTCCACGGGCCTGTTATTGCTTCAGCTGAAGTTAGAAAGGGACGACAACAAACACATGTTCTCTGAATTCTATATAGTGCCTCTACTTGACGACTGA

Protein

MAEDSSQYHFTASMDSGKTLCSLLKAIQFQESAVFCALPEGLKLTVEEGKCVQASAYIPADNFTEYHVRDDVDVIFKISLAVLAECLNIFGSGEEASLKMYYKCEGSPLLLVLQPQSFRNVMTDCEIWSQTPDSMLELRSPEETDVAKLVVRAGALLAVLGDVERSADVLELRLQPEPPHFTIVTYGMQDRSCIEVPECSDTLQSFSCERELTLKYQLHHLRLCMKALAISNKVVLRCSSTGLLLLQLKLERDDNKHMFSEFYIVPLLDD

Description

Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. Isoform 1 possesses 3'->5' double stranded DNA exonuclease activity (By similarity).

Catalytic Activity

Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.

Subunit

Component of the toroidal 9-1-1 (RAD9-RAD1-HUS1) complex, composed of RAD9A, RAD1 and HUS1. The 9-1-1 complex associates with LIG1, POLB, FEN1, RAD17, HDAC1, RPA1 and RPA2. The 9-1-1 complex associates with the RAD17-RFC complex. RAD1 interacts with POLB, FEN1, HUS1, HUS1B, RAD9A and RAD9B. Interacts with DNAJC7 (By similarity).

Similarity

Belongs to the rad1 family.

Keywords

Alternative splicing   Complete proteome   DNA damage   DNA repair   Exonuclease   Hydrolase   Nuclease   Nucleus   Reference proteome  

Feature

chain  Cell cycle checkpoint protein RAD1
splice variant  In isoform 2.

Uniprot

A0A1E1W2F6   A0A2H1VY32   A0A2A4J6D1   S4NMZ8   A0A437ARY1   A0A212F278   + More
A0A0N1IDA7   A0A194PIQ4   D6X3Z8   A0A1W4WCV2   A0A3Q4AAP1   A0A154P6B5   A0A3Q1IQ40   A0A3P8WWP6   A0A0L8FK96   A0A226PI72   C1JXM2   A7T5Z1   A0A210Q0J5   A0A088AEC1   A0A3B4WYG8   A0A1D5PSK5   A0A1S3K1Z2   A0A1B6DL23   A0A3B5KJE7   A0A3B4BJ66   A0A3Q1GS54   G3PVY0   A0A3Q1B7P3   A0A3P8TH84   A0A2A3ERR4   A0A2U9CFL8   A0A2U9CKW4   A0A3B4UWK8   A0A232FJV2   C3XV97   A0A3Q3FXG3   A0A3P9HTH5   H2LMW7   A0A3P9K7N5   A0A3B4ZIR0   A0A2J7Q1I2   A0A0F8CAP2   W8B5C0   A0A3M0LBQ3   U3JHD7   A0A2Z5U607   A0A034W1M4   A0A444U5D4   A7SU20   H0YUP7   A0A3L8S8T6   A0A1V4KDN1   I3JLK1   A0A3Q3MMV4   R7V578   A0A3Q3CVX6   A0A3Q3K8P5   K7IZC6   A0A2I0LR64   A0A0B6ZTE3   Q9QWZ1   A0A0B6ZRK9   A0A1A7WEU2   A0A067QQH9   A0A3P8NSE4   A0A3P9CW37   A0A3B3YYM5   A0A087XSC6   A0A3B3TWE6   A0A2I4BAI1   H2YAI1   A0A3Q2EA67   G3HKF5   A9ULD8   A0A3P9PVL8   A0A099Z1L3   A0A3L7I509   A0A250YEE3   A0A1U8C7T6   D3ZC52   A0A2I0UBD2   A0A091LYM9   C1BZ21   A0A3B4FK40   A0A218UVJ5   Q7ZWD7   A0A3Q3ARC2   Q6P2T4   A0A147A229   A0A3B4BVV1   A0A1A8RBS4   A0A060YHT7   A0A3Q2Q9K7   A0A1A8QW88   A0A1S3FCS8   A0A093QFT4  

EC Number

3.1.11.2

Pubmed

23622113    22118469    26354079    18362917    19820115    24487278    + More
24621616    17615350    28812685    15592404    21551351    25463417    28648823    18563158    17554307    25835551    24495485    26760975    25348373    20360741    30282656    25186727    23254933    20075255    23371554    9716408    9828137    9878245    9660799    9705507    16141072    15489334    24845553    21804562    20431018    29704459    28087693    15057822    15632090    20433749    25069045    23594743    24755649   

EMBL

GDQN01011450    GDQN01009898    GDQN01005752    GDQN01004168    GDQN01001002    JAT79604.1    + More
JAT81156.1    JAT85302.1    JAT86886.1    JAT90052.1    ODYU01005159    SOQ45750.1    NWSH01002689    PCG67677.1    GAIX01014161    JAA78399.1    RSAL01001726    RVE40723.1    AGBW02010783    OWR47837.1    KQ461178    KPJ07761.1    KQ459602    KPI93202.1    KQ971379    EEZ97345.1    KQ434827    KZC07469.1    KQ429968    KOF64876.1    AWGT02000073    OXB79159.1    FJ705223    ACO52484.1    DS471358    EDO28618.1    NEDP02005302    OWF42256.1    AC192388    GEDC01010914    JAS26384.1    KZ288192    PBC34397.1    CP026258    AWP15391.1    AWP15392.1    NNAY01000112    OXU30863.1    GG666468    EEN68023.1    NEVH01019409    PNF22435.1    KQ042382    KKF15334.1    GAMC01010215    JAB96340.1    QRBI01000102    RMC16607.1    AGTO01011146    FX985771    BBA93658.1    GAKP01009481    GAKP01009480    JAC49471.1    SCEB01215283    RXM30402.1    DS469806    EDO32797.1    ABQF01020732    QUSF01000044    RLV98173.1    LSYS01003582    OPJ82463.1    AERX01026660    AMQN01000734    KB295062    ELU13679.1    AKCR02000130    PKK19919.1    HACG01024120    CEK70985.1    AF074718    AF073523    AF076842    AJ004976    AF038841    AF058394    AF084514    AK146533    AK148098    AK150466    AK168588    BC048693    HACG01024122    CEK70987.1    HADW01002845    HADX01011989    SBP04245.1    KK853042    KDR12148.1    AYCK01007596    JH000458    EGV92308.1    AAMC01097948    BC157217    AAI57218.1    KL888260    KGL75611.1    RAZU01000096    RLQ73298.1    GFFW01002790    JAV41998.1    AC128789    CH474058    EDL82989.1    KZ505910    PKU43354.1    KK515829    KFP64708.1    BT079850    ACO14274.1    MUZQ01000116    OWK57793.1    BC049464    AAH49464.1    CR848814    BC064305    AAH64305.1    GCES01013712    JAR72611.1    HAEI01006551    HAEH01015810    SBS03525.1    FR908876    CDQ88690.1    HAEG01014319    SBR97284.1    KL693574    KFW87803.1   

Proteomes

UP000218220    UP000283053    UP000007151    UP000053240    UP000053268    UP000007266    + More
UP000192223    UP000261620    UP000076502    UP000265040    UP000265120    UP000053454    UP000198419    UP000001593    UP000242188    UP000005203    UP000261360    UP000000539    UP000085678    UP000005226    UP000261520    UP000257200    UP000007635    UP000257160    UP000265080    UP000242457    UP000246464    UP000261420    UP000215335    UP000001554    UP000261660    UP000265200    UP000001038    UP000265180    UP000261400    UP000235965    UP000269221    UP000016665    UP000007754    UP000276834    UP000190648    UP000005207    UP000261640    UP000014760    UP000264840    UP000261600    UP000002358    UP000053872    UP000000589    UP000027135    UP000265100    UP000265160    UP000261480    UP000028760    UP000261500    UP000192220    UP000007875    UP000265020    UP000001075    UP000008143    UP000242638    UP000053641    UP000273346    UP000189706    UP000002494    UP000265140    UP000261460    UP000197619    UP000264800    UP000000437    UP000261440    UP000193380    UP000265000    UP000081671    UP000053258   

PRIDE

Q9QWZ1    D3ZC52   

Pfam

PF02144   Rad1

Interpro

IPR003011   Cell_cycle_checkpoint_Rad1        + More
IPR003021   Rad1_Rec1_Rad17       
IPR007527   Znf_SWIM       

ProteinModelPortal

A0A1E1W2F6   A0A2H1VY32   A0A2A4J6D1   S4NMZ8   A0A437ARY1   A0A212F278   + More
A0A0N1IDA7   A0A194PIQ4   D6X3Z8   A0A1W4WCV2   A0A3Q4AAP1   A0A154P6B5   A0A3Q1IQ40   A0A3P8WWP6   A0A0L8FK96   A0A226PI72   C1JXM2   A7T5Z1   A0A210Q0J5   A0A088AEC1   A0A3B4WYG8   A0A1D5PSK5   A0A1S3K1Z2   A0A1B6DL23   A0A3B5KJE7   A0A3B4BJ66   A0A3Q1GS54   G3PVY0   A0A3Q1B7P3   A0A3P8TH84   A0A2A3ERR4   A0A2U9CFL8   A0A2U9CKW4   A0A3B4UWK8   A0A232FJV2   C3XV97   A0A3Q3FXG3   A0A3P9HTH5   H2LMW7   A0A3P9K7N5   A0A3B4ZIR0   A0A2J7Q1I2   A0A0F8CAP2   W8B5C0   A0A3M0LBQ3   U3JHD7   A0A2Z5U607   A0A034W1M4   A0A444U5D4   A7SU20   H0YUP7   A0A3L8S8T6   A0A1V4KDN1   I3JLK1   A0A3Q3MMV4   R7V578   A0A3Q3CVX6   A0A3Q3K8P5   K7IZC6   A0A2I0LR64   A0A0B6ZTE3   Q9QWZ1   A0A0B6ZRK9   A0A1A7WEU2   A0A067QQH9   A0A3P8NSE4   A0A3P9CW37   A0A3B3YYM5   A0A087XSC6   A0A3B3TWE6   A0A2I4BAI1   H2YAI1   A0A3Q2EA67   G3HKF5   A9ULD8   A0A3P9PVL8   A0A099Z1L3   A0A3L7I509   A0A250YEE3   A0A1U8C7T6   D3ZC52   A0A2I0UBD2   A0A091LYM9   C1BZ21   A0A3B4FK40   A0A218UVJ5   Q7ZWD7   A0A3Q3ARC2   Q6P2T4   A0A147A229   A0A3B4BVV1   A0A1A8RBS4   A0A060YHT7   A0A3Q2Q9K7   A0A1A8QW88   A0A1S3FCS8   A0A093QFT4  

PDB

3A1J     E-value=5.37625e-34,     Score=359

KEGG

101745317    K02830 cell cycle checkpoint protein [EC:3.1.11.2] | (RefSeq) cell cycle checkpoint protein RAD1

GO

GO:0005634   GO:0000077   GO:0030896   GO:0008408   GO:0008270   GO:0005654   GO:0071479   GO:0051598   GO:0005694   GO:0004527   GO:0043231   GO:0008853   GO:0006281   GO:0006810   GO:0006412   GO:0006414   GO:0004816   GO:0005737   GO:0006421   GO:0016155   GO:0003824  

PANTHER

PTHR10870  

Subcellular location

Nucleus  

Length:

270

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.09307

Exp number, first 60 AAs:

0.00111

Total prob of N-in:

0.03504

outside

1  -  270

 

 

Pi

248.214437

Theta

152.489647

Tajima's D

2.642859

CLR

0.114002

CSRT

0.954752262386881

Interpretation

Uncertain