Gene

KWMTBOMO09832  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA013129

Annotation

PREDICTED:_glucosylceramidase-like_isoform_X3_[Bombyx_mori]

Location

Bomo_Chr16(+):9971223-9983550      View in EpiBrowser

Full name

Glucosylceramidase      

Location in the cell

Cytoplasmic   Reliability : 1.094 PlasmaMembrane   Reliability : 1.317

CDS

ATGATCAAACGTAACTTATTAGTTGTGGGATTTGAGATCTCACTGTTGTTTAGCTTTGTTGTTGGATATTATAGGGATAAAACATGCGCCGAGCTGCAAGTAAACAATAGCTCCGTGATATGCGTCTGCAATGCAACGTACTGTGACACTGTGACTAGAGAGGAGCCGCACCCTAGAAGATTTATGGTGTATACTTCCTCTAAGGCAGGACTGCGGTTCAAGAAGTTCACATCTATCTTGGAGGTCTTCGATTTGTTAACAGAATGTTCTACAACCTTAGAACTCGAACCCAGAAAGAGGTATCAGACGATCCATGGCTTCGGAGGCGCCATCACCGATTCAGCGGCCATCAATTGGAGCAACTTAAAAGATGAAGAATTAAAGCAGAATTTAATAGAATCCTACTGCGGTGACAGCGGAATACAATACAACATGCTCCGCGTGCCGATCGGTGGAACCGACTTCTCACCTCGCTTCTACACATACCAGGACTACCCCGAGAGAGACAAGGACTTAAGCAACTTCACACTTGCTCCCGAGGACTTGAACTATAAGCTGCCGATGATAAAAGCCTGCATGAAAGCGTCAAATGCCTCAATAGATATCCTAGGATCGTTATGGTATCCACCTGCGTGGATGAAGAGTACGAAAAAATCGAACGGAATTTCTTTTCTGGAGGAGGAATATTATGACGCTCTCGCCCTGTATCATCTCAAATTTATCAAAGAGTATTACAAACAGGGCGTTCCGATTTGGGGCATAACTACAACGAATGAACCTTTGAGCGGGATTACAAAACATGCTAATGATAATGTACTCGGCTGGTCGGTTTACGGATTGGCAAATTGGATTGTCAATTATTTGGCACCAAAAATAAAACGGTACGATCCAAGCATCAAGATACTAGGAGTTGACGACCAGAGATTTACTGTTCCACTATGGTTCAATGCGGCATTAAAACATGTACCGGAACTAGGGAGAGCTATTGACGGGATCGGACTACATTACTATGAGAACGAACTCGTCCCGCCCGAGATAGTCGACCGATGTCTTAAAAATTATCCTAATCTGTTCGTTTTGTCTTCAGAAGCATCTATACATTCACAAACAAATAATAAAGTAGATTTAGGATCGTGGGATCGAGCTGAAAAATACATTAAGAATATAATTGAGGACCTAAATCACAACTACACAGCCTGGATCGATTGGAACGTCTGTCTTGACAAAATGGGGGGGCCTGTTTTGTCCAAAACCTATCTGGACTCGCCCGTGATTGTGGACCCTGATGCAGGGGTATTCTACAAACAGCCGTTGTTCTACGCTATGGGTCACTTCTCAAAGTTCATCCCTAAAGGTTCTAAAAGGATCCAGTCCATGGAGAAGTACAATTGTGGGGATCCCATAAAGCATGTAGCGTTTTCGACTCCCATGAATACAATAGTTGTCGTTGTTTTTAATGATAAGAATAAAGAGAAAGTGGTACGTCTGAAGCTGGGATACAGCCAAGCGAAGGTCCTGTTAGAGAAGAATTCGATTGCGACAGTCGAATTTCATAATGACGTAATCGACGAATCATGTGACTGTGAGAAGTAG

Protein

MIKRNLLVVGFEISLLFSFVVGYYRDKTCAELQVNNSSVICVCNATYCDTVTREEPHPRRFMVYTSSKAGLRFKKFTSILEVFDLLTECSTTLELEPRKRYQTIHGFGGAITDSAAINWSNLKDEELKQNLIESYCGDSGIQYNMLRVPIGGTDFSPRFYTYQDYPERDKDLSNFTLAPEDLNYKLPMIKACMKASNASIDILGSLWYPPAWMKSTKKSNGISFLEEEYYDALALYHLKFIKEYYKQGVPIWGITTTNEPLSGITKHANDNVLGWSVYGLANWIVNYLAPKIKRYDPSIKILGVDDQRFTVPLWFNAALKHVPELGRAIDGIGLHYYENELVPPEIVDRCLKNYPNLFVLSSEASIHSQTNNKVDLGSWDRAEKYIKNIIEDLNHNYTAWIDWNVCLDKMGGPVLSKTYLDSPVIVDPDAGVFYKQPLFYAMGHFSKFIPKGSKRIQSMEKYNCGDPIKHVAFSTPMNTIVVVVFNDKNKEKVVRLKLGYSQAKVLLEKNSIATVEFHNDVIDESCDCEK

Catalytic Activity

a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-acylsphing-4-enine + D-glucose

Similarity

Belongs to the glycosyl hydrolase 30 family.

Feature

chain  Glucosylceramidase

Uniprot

H9JUB6   H9JUB7   A0A2A4K975   A0A2H1VUS0   A0A1W1EGM7   A0A194PPY1   + More
A0A212F254   A0A437BUD8   A0A2H1X380   A0A2H1VST3   A0A212F248   A0A067QM95   A0A437B0B5   A0A212F293   A0A194Q614   A0A139WEE1   A0A0N1IF15   A0A0C9QXG4   A0A2H1VG50   D6WRN0   A0A2J7R6J1   A0A139WEN3   A0A131YKA6   A0A2H1WWW7   A0A224YYV0   A0A131Z3G7   A0A224YXV5   L7M0C6   L7M0B5   A0A1E1X9A0   A0A1B6FTD6   A0A1B6IGC1   D6WRR5   A0A1B6K1N7   A0A151WMN1   A0A088APM4   A0A154P4F4   A0A1W4WRP8   A0A1W2W3C7   A0A2A3EMA9   E0VQX9   A0A158NXS0   F4X220   H2YMC3   A0A1B6CTI6   F7AA01   A0A131YJ49   D6WRR4   A0A195ECY0   A0A232EVH8   A0A1W2WHS5   A0A224YX30   A0A1Y1L8U4   A0A1L8FDF0   H2YNM8   H2XJY8   J3JXY3   H2XQ11   K7IX35   A0A1V9X5I8   A0A195B051   A0A0L7QMS7   A0A0P5V115   A0A1Z5LER5   A0A088APM5   V4B2P4   A0A131ZAG4   A0A1Z5L9E9   A0A016WL53   A0A226EKU6   C3YQ03   A0A0P5D4A0   A0A0P6HWY3   A0A0P6CTA3   A0A016WKE4   A0A0P5V259   A0A0P5XL26   A0A154P6S3   A0A0P5BE18   A0A0P5MXS5   A0A1S3HF23   A0A368H7B4   A0A3Q4GE16   A0A2A3EKU2   A0A0P6E3A7   A0A1S0U9S6   E7EZM1   A0A210PEG6   A0A162QRA1   A0A0P5VAG5   A0A0N8C3M9   A0A195CKU4   V9IJV6   A0A3P6TRN0   A0A423SIH3   A0A1D2MYE0   A0A0N8A576   A0A026WL55   A0A0P5BMC7  

EC Number

3.2.1.45

Pubmed

19121390    28680679    26354079    22118469    24845553    18362917    + More
19820115    26830274    28797301    25576852    28503490    20566863    21347285    21719571    12481130    15114417    28648823    28004739    27762356    22516182    20075255    28327890    28528879    23254933    25730766    18563158    25186727    23594743    28812685    27289101    24508170    30249741   

EMBL

BABH01038539    BABH01038540    BABH01038541    BABH01038531    BABH01038532    BABH01038533    + More
BABH01038534    NWSH01000035    PCG80448.1    ODYU01004571    SOQ44585.1    LT635665    SGZ49395.1    KQ459602    KPI93190.1    AGBW02010783    OWR47828.1    RSAL01000008    RVE53975.1    ODYU01013051    SOQ59702.1    ODYU01004232    SOQ43893.1    OWR47794.1    KK853153    KDR10552.1    RSAL01000232    RVE43819.1    OWR47849.1    KQ459439    KPJ00977.1    KQ971354    KYB26348.1    KQ461178    KPJ07773.1    GBYB01005352    JAG75119.1    ODYU01002190    SOQ39362.1    EFA07058.2    NEVH01006756    PNF36452.1    KYB26349.1    GEDV01009569    JAP78988.1    ODYU01011672    SOQ57565.1    GFPF01008008    MAA19154.1    GEDV01003886    JAP84671.1    GFPF01008007    MAA19153.1    GACK01007712    JAA57322.1    GACK01007727    JAA57307.1    GFAC01003537    JAT95651.1    GECZ01016297    JAS53472.1    GECU01021735    JAS85971.1    EFA05962.2    GECU01002337    JAT05370.1    KQ982934    KYQ49129.1    KQ434814    KZC06829.1    KZ288219    PBC32336.1    DS235442    EEB15785.1    ADTU01003452    ADTU01003453    ADTU01003454    ADTU01003455    GL888561    EGI59502.1    GEDC01023766    GEDC01020570    GEDC01011572    GEDC01003214    JAS13532.1    JAS16728.1    JAS25726.1    JAS34084.1    EAAA01001913    GEDV01009570    JAP78987.1    EFA05963.2    KQ979074    KYN23063.1    NNAY01001997    OXU22351.1    GFPF01008005    MAA19151.1    GEZM01063170    JAV69258.1    CM004480    OCT69622.1    EAAA01002615    BT128105    AEE63066.1    AAZX01006377    AAZX01006727    MNPL01023560    OQR68676.1    KQ976692    KYM77670.1    KQ414868    KOC59952.1    GDIP01105548    JAL98166.1    GFJQ02001071    JAW05899.1    KB200701    ESP00722.1    GEDV01001091    JAP87466.1    GFJQ02003035    JAW03935.1    JARK01000241    EYC39763.1    LNIX01000003    OXA58323.1    GG666539    EEN57641.1    GDIP01163230    LRGB01000311    JAJ60172.1    KZS19886.1    GDIQ01028607    JAN66130.1    GDIP01009481    JAM94234.1    KE124952    EPB74230.1    EYC39762.1    GDIP01105549    JAL98165.1    GDIP01070494    JAM33221.1    KZC06830.1    GDIP01190080    JAJ33322.1    GDIQ01149727    JAL01999.1    JOJR01000007    RCN52482.1    PBC32337.1    GDIQ01068405    JAN26332.1    JH712071    EFO26573.2    BX322530    NEDP02076748    OWF34883.1    KZS19887.1    GDIP01102691    JAM01024.1    GDIQ01118027    JAL33699.1    KQ977622    KYN01328.1    JR049360    AEY60937.1    UYRX01000558    VDK83835.1    QCYY01003347    ROT63991.1    LJIJ01000420    ODM97675.1    GDIP01181329    JAJ42073.1    KK107159    QOIP01000001    EZA56663.1    RLU27086.1    GDIP01182978    JAJ40424.1   

Proteomes

UP000005204    UP000218220    UP000053268    UP000007151    UP000283053    UP000027135    + More
UP000007266    UP000053240    UP000235965    UP000075809    UP000005203    UP000076502    UP000192223    UP000242457    UP000009046    UP000005205    UP000007755    UP000007875    UP000008144    UP000078492    UP000215335    UP000186698    UP000002358    UP000192247    UP000078540    UP000053825    UP000030746    UP000024635    UP000198287    UP000001554    UP000076858    UP000085678    UP000252519    UP000261580    UP000000437    UP000242188    UP000078542    UP000277928    UP000283509    UP000094527    UP000053097    UP000279307   

PRIDE

H9JUB6    H9JUB7   

Pfam

PF17189   Glyco_hydro_30C        + More
PF02055   Glyco_hydro_30
PF16087   DUF4817

Interpro

IPR001139   Glyco_hydro_30        + More
IPR013780   Glyco_hydro_b       
IPR033453   Glyco_hydro_30_TIM-barrel       
IPR033452   GH30_C       
IPR017853   Glycoside_hydrolase_SF       
IPR032135   DUF4817       

SUPFAM

SSF51445   SSF51445       

Gene 3D

2.60.40.1180    

ProteinModelPortal

H9JUB6   H9JUB7   A0A2A4K975   A0A2H1VUS0   A0A1W1EGM7   A0A194PPY1   + More
A0A212F254   A0A437BUD8   A0A2H1X380   A0A2H1VST3   A0A212F248   A0A067QM95   A0A437B0B5   A0A212F293   A0A194Q614   A0A139WEE1   A0A0N1IF15   A0A0C9QXG4   A0A2H1VG50   D6WRN0   A0A2J7R6J1   A0A139WEN3   A0A131YKA6   A0A2H1WWW7   A0A224YYV0   A0A131Z3G7   A0A224YXV5   L7M0C6   L7M0B5   A0A1E1X9A0   A0A1B6FTD6   A0A1B6IGC1   D6WRR5   A0A1B6K1N7   A0A151WMN1   A0A088APM4   A0A154P4F4   A0A1W4WRP8   A0A1W2W3C7   A0A2A3EMA9   E0VQX9   A0A158NXS0   F4X220   H2YMC3   A0A1B6CTI6   F7AA01   A0A131YJ49   D6WRR4   A0A195ECY0   A0A232EVH8   A0A1W2WHS5   A0A224YX30   A0A1Y1L8U4   A0A1L8FDF0   H2YNM8   H2XJY8   J3JXY3   H2XQ11   K7IX35   A0A1V9X5I8   A0A195B051   A0A0L7QMS7   A0A0P5V115   A0A1Z5LER5   A0A088APM5   V4B2P4   A0A131ZAG4   A0A1Z5L9E9   A0A016WL53   A0A226EKU6   C3YQ03   A0A0P5D4A0   A0A0P6HWY3   A0A0P6CTA3   A0A016WKE4   A0A0P5V259   A0A0P5XL26   A0A154P6S3   A0A0P5BE18   A0A0P5MXS5   A0A1S3HF23   A0A368H7B4   A0A3Q4GE16   A0A2A3EKU2   A0A0P6E3A7   A0A1S0U9S6   E7EZM1   A0A210PEG6   A0A162QRA1   A0A0P5VAG5   A0A0N8C3M9   A0A195CKU4   V9IJV6   A0A3P6TRN0   A0A423SIH3   A0A1D2MYE0   A0A0N8A576   A0A026WL55   A0A0P5BMC7  

PDB

2XWE     E-value=3.42689e-76,     Score=726

KEGG

101742786    K01201 glucosylceramidase [EC:3.2.1.45] | (RefSeq) glucosylceramidase-like

PATHWAY

00511    Other glycan degradation - Bombyx mori (domestic silkworm)
00600    Sphingolipid metabolism - Bombyx mori (domestic silkworm)
01100    Metabolic pathways - Bombyx mori (domestic silkworm)
04142    Lysosome - Bombyx mori (domestic silkworm)

GO

GO:0006665   GO:0004348   GO:0006680   GO:0016787   GO:0016021   GO:0070050   GO:0005623   GO:0030278   GO:0036268   GO:0005975   GO:0008270   GO:0005488   GO:0008565   GO:0015031   GO:0009258   GO:0016155   GO:0003824  

PANTHER

PTHR11069  

Length:

530

Number of predicted TMHs:

1

Exp number of AAs in TMHs:

15.94075

Exp number, first 60 AAs:

15.83974

Total prob of N-in:

0.83334

POSSIBLE N-term signal

sequence

inside

1  -  6

TMhelix

7  -  24

outside

25  -  530

 

 

Pi

245.274042

Theta

174.911025

Tajima's D

0.282785

CLR

150.305411

CSRT

0.451827408629568

Interpretation

Uncertain