Gene

KWMTBOMO09804  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA013115

Annotation

phenoloxidase_subunit_2_precursor_[Bombyx_mori]

Location

Bomo_Chr16(+):9607829-9622089      View in EpiBrowser

Full name

Phenoloxidase subunit 2       + More
Phenoloxidase 1      
Phenoloxidase subunit 1      
Phenoloxidase 2      

Alternative Name

PO 2
Tyrosinase 2
proPO-p2
Prophenoloxidase-I
PO 1
Tyrosinase 1
proPO-P1
Phenoloxidase subunit A3
Processed phenoloxidase A3

Location in the cell

Nuclear   Reliability : 2.165

CDS

ATGGCTGACGTTTTTGAAAGCCTCGAGTTGCTGTTCGATCGTCCAAATGAGCCGCTCATCACACCCAAGGGCGAAAACAATTCTGTGTTTCAACTCACAGAACAATTTTTGACTGAGGACTACGCCAACAACGGCATCGAATTAAACAACCGTTTCGGTGACGATGCTTCTGAGAAGATACCCCTCAAGAACCTCAGCAAACTCCCAGAATTTAAAATTGCAACTCAACTACCCAAGGACGCTGAATTCTCATTGTTTCTACCTAAACATCAAGAAATGGCAAATGAACTTCTTGGCGTTCTCATGGATGTACCAGAGAACGAATTACAAGATTTGCTATCGACATGCGCCTTTGCACGAGTAAACCTGAACCCTCAGTTGTTCAACTATTGTTACTCTGTGGCACTGATGCACAGACGTGATACCAGAAAAGTCAGAGTTAAGAATTTTGCAGAAGTATTTCCCTCTAAATTCTTGGATTCCCAAGTATTCACTCAGGCTCGTGAAACTGCAGCCGTTATTCCACCAGATGTTCCACGCATACCTATTATCATTCCACGAGACTACACTGCGACGGACTTAGAAGAAGAACACCGCCTTGCGTACTGGCGAGAAGATATCGGCATCAACCTGCATCATTATCACTGGCATTTGGTCTACCCGTTTACGGCCAACGATCTTTCAATCGTAGCTAAGGACCGTCGTGGGGAGTTGTTCTTCTATATGCACCAGCAAGTGATAGCCCGCTTCAACTGCGAGCGCTTGTGCAATTCATTAAAAAGAGTTAAAAAATTCAGCAACTGGAGGGAGCCAATTCCAGAGGCTTACTTCCCTAAGCTTGACAGTTTGACTTCGTCGCGCGGATGGCCGCCGCGTCAGTCCGGTATGCAATGGCAGGACCTGAATCGTGCGGCCGAAGGTCTATTTGTGACCATTGACGAAATGGAACGCTGGAGAAGAAACGTTGAAGAAGCTATCGCGACTGGTACCGTTAGATTGCCAAACGGGCAGACTCGGCCTCTTGACATCGATACGCTCGGCAACATGTTGGAGTCCAGCGCCCTCTCGCCGAACAGAGAACTGTACGGTTCGATACACAACAACGGGCACAGTTTCACCGCGTACATGCACGACCCGGAACACAGATACCTTGAACAATTTGGTGTTATCGCTGATGAGGCCACAACGATGCGCGATCCATTCTTCTACCGCTGGCACGCCTACATCGATGACGTTTTCCAGAAGCACAAGGAATCTGCCTACGTGCGTCCTTACACTCGATCTGAGCTCGAGAACCCGGGCGTGCAAGTGAGGTCCGTGTCGGTGGAGACGCCCGGCGGACAACCGAACACGCTGAACACGTTCTGGATGCTCAGCGACGTGAACTTGTCGCGCGGACTCGACTTCTCCGACAACGGGCCCGTGTACGCTCGCTTCACTCACCTCAACTACAGGCATTTCAGCTACAGAATAAACGTGAACAACACCGGTAGCAGCCGTCGCACCACAGTGCGTATCTTCATCACTCCGAAGTTCGACGAGCGCAATGTCCCCTGGATATTCTCGGACCAACGCAAGATGTGTATTGAAATGGACAGATTCGTCACTGTCCTGAACGCCGGAGAGAATAATATTGTCCGTCAGTCGACGGAGTCTTCCATAACTATTCCGTTCGAACAGACATTCCGCGACCTATCCGCTCAGGGCAATGATCCACGACGCGACGAACTCGCTACCTTCAATTACTGCGGCTGCGGCTGGCCTCAGCACATGCTCGTGCCCAAGGGCACTGAAGCCGGCATGCCCTTCCAACTGTTTGTTATGTTATCCAACTATGATTTAGACCGGATCGATCAAGATGACGGAAAACAGCTCACTTGTGTGGAAGCGTCGAGCTTCTGTGGATTAAAGGATAAGAAATATCCTGATAGGCGCGCTATGGGATTCCCGTTCGATCGACCCTCGAGCAGCGCCACCAGCCTCCAGGACTTCATTTTACCAAACATGGGCTTGCAGGACATCACTATTCAATTGCAAAACGTCACCGAACCTAACCCACGGAACCCTCCCATGTCTGTTTAA

Protein

MADVFESLELLFDRPNEPLITPKGENNSVFQLTEQFLTEDYANNGIELNNRFGDDASEKIPLKNLSKLPEFKIATQLPKDAEFSLFLPKHQEMANELLGVLMDVPENELQDLLSTCAFARVNLNPQLFNYCYSVALMHRRDTRKVRVKNFAEVFPSKFLDSQVFTQARETAAVIPPDVPRIPIIIPRDYTATDLEEEHRLAYWREDIGINLHHYHWHLVYPFTANDLSIVAKDRRGELFFYMHQQVIARFNCERLCNSLKRVKKFSNWREPIPEAYFPKLDSLTSSRGWPPRQSGMQWQDLNRAAEGLFVTIDEMERWRRNVEEAIATGTVRLPNGQTRPLDIDTLGNMLESSALSPNRELYGSIHNNGHSFTAYMHDPEHRYLEQFGVIADEATTMRDPFFYRWHAYIDDVFQKHKESAYVRPYTRSELENPGVQVRSVSVETPGGQPNTLNTFWMLSDVNLSRGLDFSDNGPVYARFTHLNYRHFSYRINVNNTGSSRRTTVRIFITPKFDERNVPWIFSDQRKMCIEMDRFVTVLNAGENNIVRQSTESSITIPFEQTFRDLSAQGNDPRRDELATFNYCGCGWPQHMLVPKGTEAGMPFQLFVMLSNYDLDRIDQDDGKQLTCVEASSFCGLKDKKYPDRRAMGFPFDRPSSSATSLQDFILPNMGLQDITIQLQNVTEPNPRNPPMSV

Description

This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA, DOPA to DOPA-quinone and possibly 5,6 dihydroxyindole to indole-5'6 quinone. Binds to the surface of hemocytes and is involved in hemocyte melanization.
This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the oxidation of o-diphenols (N-acetyldopamine, 4-methylcatechol and dopamine). Cannot oxidize monophenols and p-phenols (L-tyrosine, tyramine, gentisic acid and hydroquinone). Binds to the surface of hemocytes and is involved in hemocyte melanization. Activation of the enzyme in response to bacterial lipopolysaccharides (LPS) suggests it may play a role in innate immunity.
This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA, DOPA to DOPA-quinone and possibly 5,6 dihydroxyindole to indole-5'6 quinonee (By similarity).

Catalytic Activity

2 L-dopa + O2 = 2 H2O + 2 L-dopaquinone
L-tyrosine + O2 = H2O + L-dopaquinone

Cofactor

Cu(2+)

Subunit

Heterodimer.
Heterodimer. Forms a complex with an interleukin 1-like protein as a consequence of a host defense response.
Dimer (PubMed:9085271). Might form a homodimer or a heterodimer with PPO1 (PubMed:9085271). Might interact with PPAF2 (via CLIP domain); the interaction might be required for PPO1 activity (PubMed:16362048).

Similarity

Belongs to the tyrosinase family.

Keywords

Complete proteome   Copper   Direct protein sequencing   Disulfide bond   Glycoprotein   Melanin biosynthesis   Metal-binding   Monooxygenase   Oxidoreductase   Reference proteome   Secreted   Zymogen   3D-structure   Immunity   Innate immunity  

Feature

chain  Phenoloxidase subunit 2
propeptide  Removed by PPAF1

Uniprot

Q27452   B2BSN7   Q9BLG6   Q9GU90   A7L8D1   A8JKF6   + More
Q2VIY6   A0S6A1   A0A3S2NKZ2   D5LPT7   R4WAN9   Q3ZPT5   Q0VJU6   Q25519   A0A173GPF1   C6K2H4   D2KI02   Q6UEH6   Q2I355   A0A194QIL4   B4XIE2   I4DJE0   A0A1E1WIY6   F1B0G0   O76208   D9J044   A0A212FJ43   A0A212FJ35   A0A1Q1MKI6   A0A1E1W965   Q49I39   A0A0L0C2T8   A0S6A0   A0A1I8ND44   Q6RSJ0   A0A1J1J6I1   R4WAL7   A0A0M4MZ77   L7UVJ1   A0A1I8Q7Q8   L7US91   J7GXB2   Q0MVN6   Q8I6K1   A0A290U614   A0A139WGK2   Q49I38   O97047   A0A1A9XS96   A0A1B0BSF2   A0A0A1WDY9   W8BTJ6   D2A424   A0A1I8QBX9   Q27451   A0A1B0AFL7   B2YKU4   H9JTA1   A0A0L0C2Z2   Q9Y0B4   E0AEY7   D6QZJ9   A0A1A9VT99   A0A0K8UHE0   A0A034V716   A0A0U2PES0   A0A1I8NH64   O44249   A0A0K8VQL9   A0A1I8PHG1   Q9GU89   A0A240SX08   Q64H43   G3ESX4   B4P3V6   A0A1A9WK14   W8CHH4   A0A0J9R8I8   Q9V521   B4HSD9   Q86MV4   A0A1I8NVC5   A0A1I8NBL4   B3N821   A0A1A9XS95   A0A0L0C2W4   A0A026WPD0   E2B6U0   A0A2A3EAF0   A0A195CM22   A0A1A9VT97   A0A3B0JMC7   A0A240SX07   A0A0C9PJA6   A0A1W4UKH8   A0A194Q050   N6UF31   A0A1B6MSZ9   A0A1B6MHI2   U4ULX0  

EC Number

1.14.18.1
1.14.18.-

Pubmed

7644494    7793973    11118441    18070669    18839417    28953952    + More
16185666    18207075    7644492    8626641    9474780    10436935    16291091    19805072    21638307    26354079    18348246    22651552    21265042    9537761    22118469    28078308    16076951    18362917    19820115    26108605    25315136    12185078    9085271    16362048    10050770    25830018    24495485    19121390    10899462    25348373    15898113    17994087    17550304    22936249    10731132    12537572    12537569    12834045    19141111    15544939    15857760    24508170    30249741    20798317    23537049   

EMBL

D49371    AB048762    BAB41101.1    EU047703    ABW06580.1    AB048761    + More
BAB40959.1    AF178461    AAG09303.1    EF684939    ABS59653.1    EF044308    NWSH01000035    ABM65701.1    PCG80455.1    DQ114946    EF614331    KY744278    AAZ52554.1    ABU98653.1    ATD13327.1    DQ289582    ABB92835.1    RSAL01000051    RVE50242.1    GU953227    ADF43211.1    AB778569    BAM76812.1    AY703825    AAW22859.1    AM293328    CAL25133.1    L42556    AAC37243.1    KT006135    ANH58160.1    GQ149238    ACS36209.1    GU214207    ADA60207.1    AY371489    AAQ75026.1    DQ333883    ABC59699.2    KQ458756    KPJ05239.1    EU046573    ABW16862.1    AK401408    BAM18030.1    GDQN01004074    JAT86980.1    HM210573    ADM53096.1    AF020391    AAC34256.1    HM235814    ADK11055.1    AGBW02008320    OWR53745.1    OWR53744.1    KX437621    AQM36766.1    GDQN01007653    JAT83401.1    AY884063    KQ971312    AAX84204.1    EEZ99189.1    JRES01000975    KNC26576.1    DQ289581    ABB92834.1    AY494738    AAR84669.1    CVRI01000070    CRL07388.1    AB778568    BAM76811.1    KP974690    ALE19971.1    JX987234    AGC54939.1    JX987235    AGC54940.1    JX110850    AFP81886.1    DQ841706    NWSH01000045    ABH10016.2    PCG80267.1    AB079665    BAC15603.1    KY744277    ATD13326.1    KQ971348    KYB26937.1    AY884064    AAX84205.1    AB020738    BAA75470.1    JXJN01019700    GBXI01017411    JAC96880.1    GAMC01009964    GAMC01009963    JAB96591.1    EFA05662.1    D49370    BAA08368.1    EU569724    ACC69184.1    BABH01004244    BABH01004245    KNC26607.1    AF161260    AAD45526.1    HM776513    ADL14486.1    HM026570    ADG65539.1    GDHF01026318    JAI25996.1    GAKP01021407    JAC37545.1    KR181955    ALJ76679.1    AF003253    AAC05796.1    GDHF01011142    JAI41172.1    AF178462    AAG09304.1    CCAG010007845    AY665397    AAU29555.1    JF342362    AEA92682.1    CM000157    EDW89439.1    JX844653    JX844654    AFU83105.1    CM002911    KMY92407.1    AE013599    AY060652    AB055857    CH480816    EDW47034.1    AY242387    AAO72539.2    CH954177    EDV59434.1    KNC26577.1    KK107139    QOIP01000003    EZA57912.1    RLU24656.1    GL446009    EFN88581.1    KZ288308    PBC28707.1    KQ977580    KYN01776.1    OUUW01000001    SPP74446.1    GBYB01001048    JAG70815.1    KQ459582    KPI98957.1    APGK01024671    KB740558    ENN80355.1    GEBQ01000915    JAT39062.1    GEBQ01004626    JAT35351.1    KB632357    ERL93468.1   

Proteomes

UP000005204    UP000218220    UP000283053    UP000053268    UP000007151    UP000007266    + More
UP000037069    UP000095301    UP000183832    UP000095300    UP000092443    UP000092460    UP000092445    UP000078200    UP000092444    UP000002282    UP000091820    UP000000803    UP000001292    UP000005203    UP000008711    UP000053097    UP000279307    UP000008237    UP000242457    UP000078542    UP000268350    UP000192221    UP000019118    UP000030742   

PRIDE

Q27452    Q9BLG6    Q9GU90    Q27451    H9JTA1    O44249    + More
Q9GU89    Q9V521    Q86MV4   

Pfam

PF00372   Hemocyanin_M        + More
PF03722   Hemocyanin_N
PF03723   Hemocyanin_C
PF01401   Peptidase_M2

Interpro

IPR013788   Hemocyanin/hexamerin        + More
IPR008922   Unchr_di-copper_centre       
IPR037020   Hemocyanin_C_sf       
IPR014756   Ig_E-set       
IPR005204   Hemocyanin_N       
IPR002227   Tyrosinase_Cu-bd       
IPR000896   Hemocyanin/hexamerin_mid_dom       
IPR005203   Hemocyanin_C       
IPR036697   Hemocyanin_N_sf       
IPR001548   Peptidase_M2       

SUPFAM

SSF81296   SSF81296        + More
SSF48050   SSF48050       
SSF48056   SSF48056       

Gene 3D

1.10.1280.10     1.20.1370.10     2.60.40.1520    

ProteinModelPortal

Q27452   B2BSN7   Q9BLG6   Q9GU90   A7L8D1   A8JKF6   + More
Q2VIY6   A0S6A1   A0A3S2NKZ2   D5LPT7   R4WAN9   Q3ZPT5   Q0VJU6   Q25519   A0A173GPF1   C6K2H4   D2KI02   Q6UEH6   Q2I355   A0A194QIL4   B4XIE2   I4DJE0   A0A1E1WIY6   F1B0G0   O76208   D9J044   A0A212FJ43   A0A212FJ35   A0A1Q1MKI6   A0A1E1W965   Q49I39   A0A0L0C2T8   A0S6A0   A0A1I8ND44   Q6RSJ0   A0A1J1J6I1   R4WAL7   A0A0M4MZ77   L7UVJ1   A0A1I8Q7Q8   L7US91   J7GXB2   Q0MVN6   Q8I6K1   A0A290U614   A0A139WGK2   Q49I38   O97047   A0A1A9XS96   A0A1B0BSF2   A0A0A1WDY9   W8BTJ6   D2A424   A0A1I8QBX9   Q27451   A0A1B0AFL7   B2YKU4   H9JTA1   A0A0L0C2Z2   Q9Y0B4   E0AEY7   D6QZJ9   A0A1A9VT99   A0A0K8UHE0   A0A034V716   A0A0U2PES0   A0A1I8NH64   O44249   A0A0K8VQL9   A0A1I8PHG1   Q9GU89   A0A240SX08   Q64H43   G3ESX4   B4P3V6   A0A1A9WK14   W8CHH4   A0A0J9R8I8   Q9V521   B4HSD9   Q86MV4   A0A1I8NVC5   A0A1I8NBL4   B3N821   A0A1A9XS95   A0A0L0C2W4   A0A026WPD0   E2B6U0   A0A2A3EAF0   A0A195CM22   A0A1A9VT97   A0A3B0JMC7   A0A240SX07   A0A0C9PJA6   A0A1W4UKH8   A0A194Q050   N6UF31   A0A1B6MSZ9   A0A1B6MHI2   U4ULX0  

PDB

3HHS     E-value=0,     Score=2834

KEGG

693073    no KO assigned | (RefSeq) PPO2, BmPPO2, pro-PO2; phenoloxidase subunit 2

GO

GO:0006952   GO:0005576   GO:0046872   GO:0006583   GO:0004503   GO:0016491   GO:0035008   GO:0005507   GO:0031404   GO:0004097   GO:0045087   GO:0004497   GO:0042438   GO:0008237   GO:0008241   GO:0016020   GO:0036264   GO:0050832   GO:0050830   GO:0035011   GO:0005615   GO:0036263   GO:0042417   GO:0035006   GO:0008152   GO:0006351   GO:0030131   GO:0005488   GO:0008565   GO:0015031   GO:0009258   GO:0016155   GO:0003824  

PANTHER

PTHR11511  

Subcellular location

Secreted  

Length:

693

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.000780000000000001

Exp number, first 60 AAs:

0

Total prob of N-in:

0.00004

outside

1  -  693

 

 

Pi

253.510415

Theta

194.289621

Tajima's D

1.114335

CLR

0.218157

CSRT

0.695565221738913

Interpretation

Uncertain