Silkworm Genome Informatics Database

Gene

KWMTBOMO09691

Annotation

PREDICTED:_iron-sulfur_cluster_co-chaperone_protein_HscB?_mitochondrial_[Papilio_polytes]

Location

Bomo_Chr16(-):7023823-7025291 View in EpiBrowser

Full name

Iron-sulfur cluster co-chaperone protein HscB

Alternative Name

DnaJ homolog subfamily C member 20

Location in the cell

Cytoplasmic Reliability : 1.768 Nuclear Reliability : 2.307

CDS

ATGGGTGTTAGTGAAACATATGATTTGAATGAAAATGATTTAGCTAAAAAATACAAAGATCTGCAAAAATATCTACACCCAGACAAATATGCTAATAGAGTCAAAGAAGAACAAGAAATATCTGAAAAATATTCTTCCTTAGTAAATGAAGCTTATAAAACATTGTTGGAACCATTAGCAAGAGGTATCTACATGCTTCAATTAAGAGGTAAAGACATTCCTGAAAAAACAGAAGTTGATCAAGAATTTCTTATGAAAATTATGGAGAAGAATGAAGAAGTAGAATCAGCAGAAACGGAGGAAGAAATCATGAATTTAAATGAGGAAAATAAAAATAAAATTCAGTCCTTACAAAAACAGTTATCAACGGCATTCTTTGAAGGTGATCTCAAAAGGGTTACCAAGTTGCTAACTCAATTAAAATATTACACCAGTATAGATAACCAAATACAGTCTGTTATACGAAGTAAAGGAATAATAAGATAA

Protein

MGVSETYDLNENDLAKKYKDLQKYLHPDKYANRVKEEQEISEKYSSLVNEAYKTLLEPLARGIYMLQLRGKDIPEKTEVDQEFLMKIMEKNEEVESAETEEEIMNLNEENKNKIQSLQKQLSTAFFEGDLKRVTKLLTQLKYYTSIDNQIQSVIRSKGIIR

Description

Acts as a co-chaperone in iron-sulfur cluster assembly in both mitochondria and the cytoplasm (PubMed:20668094, PubMed:29309586). Required for incorporation of iron-sulfur clusters into SDHB, the iron-sulfur protein subunit of succinate dehydrogenase that is involved in complex II of the mitochondrial electron transport chain (PubMed:26749241). Recruited to SDHB by interaction with SDHAF1 which first binds SDHB and then recruits the iron-sulfur transfer complex formed by HSC20, HSPA9 and ISCU through direct binding to HSC20 (PubMed:26749241). Also mediates complex formation between components of the cytosolic iron-sulfur biogenesis pathway and the CIA targeting complex composed of CIAO1, DIPK1B/FAM69B and MMS19 by binding directly to the scaffold protein ISCU and to CIAO1 (PubMed:29309586). This facilitates iron-sulfur cluster insertion into a number of cytoplasmic and nuclear proteins including POLD1, ELP3, DPYD and PPAT (PubMed:29309586).

Subunit

Self-interacts (PubMed:29309586). Interacts with ISCU and HSPA9 to form an iron-sulfur transfer complex (PubMed:20668094). Interacts with SDHAF1 (via the first LYR motif); the interaction recruits the iron-sulfur transfer complex composed of HSC20, HSPA9 and ISCU and mediates the incorporation of iron-sulfur clusters into SDHB which also interacts with HSC20 (PubMed:26749241). Interacts with the cytoplasmic form of ISCU and with CIA complex member CIAO1 (via LYR motif) (PubMed:29309586).

Similarity

Belongs to the peptidase S1 family.
Belongs to the HscB family.

Keywords

3D-structure Chaperone Complete proteome Cytoplasm Metal-binding Mitochondrion Polymorphism Reference proteome Transit peptide

Feature

chain Iron-sulfur cluster co-chaperone protein HscB, cytoplasmic
sequence variant In dbSNP:rs17886090.

Uniprot

A0A2A4JDF3 A0A212FN23 A0A437B8N3 A0A0L7L2M0 A0A2H1VIX1 A0A194PZV2 + More
A0A194R252 E2B3T7 K7JK10 A0A026WRN4 A0A232FCT3 A0A0L7R7Y9 A0A195E650 A0A2A3EDI9 A0A158NQR4 F4WP17 A0A195CN34 A0A195EU04 A0A151WM74 A0A088AJ48 A0A1Y1LXF8 A0A1W4WVU3 A0A067R3P6 A0A2R9ALN2 H2QLF2 K7CWN5 G3RLQ2 A0A452RB25 A0A3Q7VC09 A0A384BRZ0 A0A0C9QXZ3 C1C499 U3CUZ7 A0A2U3VX99 A0A2K5JEZ7 A0A3Q7PDR2 A0A287AIR5 D2H244 A0A2Y9I676 A0A2U3YD29 C3XVD9 L5M1C6 A0A3Q1HC86 A0A154NYJ9 A0A091DLD0 A0A2K6MBS0 A0A2K6R0X8 A0A0B4KJS2 A0A2K5E0B1 U3F5K6 A0A2K5KQU6 A0A2K5YRL0 G7N3I2 H9F4A7 A0A1D5Q5I4 A0A3P8V5Z0 G7PHH0 I2CV96 A0A2K5U2E7 A0A0D9RC58 A0A383Z6Z2 A0A2P8Z984 U6CV53 G9K4T4 L5L5C4 G1PUZ0 A0A2R8M6R9 A0A2Y9KWY9 G1T125 H2P3X7 C1BWA0 A0A2I2U3M8 M3YQ49 G1QPI3 C4A116 A0A096NRI2 A0A384NYJ4 Q8IWL3 A0A3Q0T5R0 A0A402FFF3 A0A1B6HTT0 A0A1B6IWU9 A0A0T6B6W2 A0A3P9KNQ1 A0A3B5BA12 L8IZL5 F1MLK9 A0A0P6J7F1 A6QLM8 A0A1B6KGD9 A0A341D9Q8 W5NXH4 F6UZ83 A0A452G4V6 A0A3Q7S991 A0A2T7NFC4 A0A1S3JYJ6 G5APY5 A0A3P9M4V1 A0A3B3HNZ8

Pubmed

22118469 26227816 26354079 20798317 20075255 24508170 + More
30249741 28648823 21347285 21719571 28004739 24845553 22722832 15164055 16136131 22398555 24813606 25243066 30723633 20010809 18563158 25362486 24792819 22002653 25319552 17431167 24487278 29403074 23236062 23258410 21993624 20433749 25069045 17975172 11181995 12938016 15461802 10591208 15489334 20668094 21269460 26749241 29309586 18713742 17554307 22751099 19393038 20809919 16341006 21993625

EMBL

NWSH01002011 PCG69443.1 AGBW02007649 OWR55123.1 RSAL01000115 RVE46901.1 + More
JTDY01003417 KOB69586.1 ODYU01002820 SOQ40778.1 KQ459582 KPI98851.1 KQ460878 KPJ11594.1 GL445407 EFN89606.1 KK107139 QOIP01000003 EZA57764.1 RLU24946.1 NNAY01000406 OXU28621.1 KQ414637 KOC66948.1 KQ979608 KYN20357.1 KZ288290 PBC29236.1 ADTU01023474 GL888243 EGI63948.1 KQ977574 KYN01877.1 KQ981965 KYN31730.1 KQ982944 KYQ48901.1 GEZM01044415 JAV78243.1 KK852921 KDR13774.1 AJFE02069022 AJFE02069023 AJFE02069024 AJFE02069025 AACZ04068128 GABE01010807 GABE01010806 NBAG03000026 JAA33932.1 PNI97734.1 GABD01003926 GABD01003925 JAA29175.1 CABD030120659 CABD030120660 GBYB01000570 JAG70337.1 BT081678 ACO51809.1 GAMR01010650 JAB23282.1 AEMK02000096 DQIR01133325 DQIR01168013 DQIR01200879 HDA88801.1 ACTA01048049 ACTA01056049 ACTA01064049 ACTA01072049 GL192437 EFB25742.1 GG666468 EEN68065.1 KB106121 ELK31493.1 KQ434777 KZC04154.1 KN123755 KFO23616.1 JX975226 GAAL01000015 AGU38789.1 JAT78649.1 GAMT01010146 GAMQ01007740 JAB01715.1 JAB34111.1 CM001262 EHH20114.1 JU325710 AFE69466.1 JSUE03005239 JSUE03005240 CM001285 EHH65726.1 JV635541 AFJ70881.1 AQIA01007397 AQIA01007398 AQIB01136879 PYGN01000141 PSN53051.1 HAAF01002764 CCP74590.1 JP011311 AER99908.1 KB030308 ELK18635.1 AAPE02038454 ABGA01144214 ABGA01144215 NDHI03003442 PNJ48975.1 BT078879 ACO13303.1 AANG04000189 AEYP01066113 AEYP01066114 AEYP01066115 ADFV01177461 ADFV01177462 GG666843 EEN41517.1 AHZZ02001055 AHZZ02001056 GQ891495 CH471095 ADO22357.1 EAW59758.1 AY191719 CR456462 AL023494 AL117330 BC065569 BDOT01000231 GCF55183.1 GECU01029620 JAS78086.1 GECU01016297 GECU01010815 JAS91409.1 JAS96891.1 LJIG01009508 KRT82939.1 JH880564 ELR60637.1 GEBF01005078 JAN98554.1 BC148020 AAI48021.1 GEBQ01029474 GEBQ01022576 JAT10503.1 JAT17401.1 AMGL01039789 AAEX03014782 LWLT01000018 PZQS01000013 PVD19878.1 JH166403 EHA99095.1

Proteomes

UP000218220 UP000007151 UP000283053 UP000037510 UP000053268 UP000053240 + More
UP000008237 UP000002358 UP000053097 UP000279307 UP000215335 UP000053825 UP000078492 UP000242457 UP000005205 UP000007755 UP000078542 UP000078541 UP000075809 UP000005203 UP000192223 UP000027135 UP000240080 UP000002277 UP000001519 UP000291022 UP000286642 UP000261680 UP000291021 UP000245340 UP000233080 UP000286641 UP000008227 UP000008912 UP000248481 UP000245341 UP000001554 UP000265040 UP000076502 UP000028990 UP000233180 UP000233200 UP000233020 UP000233060 UP000233140 UP000006718 UP000265120 UP000009130 UP000233100 UP000029965 UP000261681 UP000245037 UP000010552 UP000001074 UP000008225 UP000248482 UP000001811 UP000001595 UP000265140 UP000011712 UP000000715 UP000001073 UP000028761 UP000005640 UP000261340 UP000288954 UP000265180 UP000261400 UP000009136 UP000252040 UP000002356 UP000002254 UP000291000 UP000286640 UP000245119 UP000085678 UP000006813 UP000001038

PRIDE

Q8IWL3

Pfam

PF00226   DnaJ        + More
PF07743   HSCB_C
PF00089   Trypsin
PF18256   HscB_4_cys

Interpro

IPR036386   HscB_C_sf        + More
IPR004640   HscB
IPR001623   DnaJ_domain
IPR036869   J_dom_sf
IPR009073   HscB_oligo_C
IPR033116   TRYPSIN_SER
IPR001254   Trypsin_dom
IPR001314   Peptidase_S1A
IPR009003   Peptidase_S1_PA
IPR018114   TRYPSIN_HIS
IPR040682   HscB_4_cys

SUPFAM

SSF46565   SSF46565        + More
SSF47144   SSF47144
SSF50494   SSF50494

Gene 3D

1.10.287.110 1.20.1280.20

CDD

cd06257 DnaJ
cd00190 Tryp_SPc

ProteinModelPortal

A0A2A4JDF3 A0A212FN23 A0A437B8N3 A0A0L7L2M0 A0A2H1VIX1 A0A194PZV2 + More
A0A194R252 E2B3T7 K7JK10 A0A026WRN4 A0A232FCT3 A0A0L7R7Y9 A0A195E650 A0A2A3EDI9 A0A158NQR4 F4WP17 A0A195CN34 A0A195EU04 A0A151WM74 A0A088AJ48 A0A1Y1LXF8 A0A1W4WVU3 A0A067R3P6 A0A2R9ALN2 H2QLF2 K7CWN5 G3RLQ2 A0A452RB25 A0A3Q7VC09 A0A384BRZ0 A0A0C9QXZ3 C1C499 U3CUZ7 A0A2U3VX99 A0A2K5JEZ7 A0A3Q7PDR2 A0A287AIR5 D2H244 A0A2Y9I676 A0A2U3YD29 C3XVD9 L5M1C6 A0A3Q1HC86 A0A154NYJ9 A0A091DLD0 A0A2K6MBS0 A0A2K6R0X8 A0A0B4KJS2 A0A2K5E0B1 U3F5K6 A0A2K5KQU6 A0A2K5YRL0 G7N3I2 H9F4A7 A0A1D5Q5I4 A0A3P8V5Z0 G7PHH0 I2CV96 A0A2K5U2E7 A0A0D9RC58 A0A383Z6Z2 A0A2P8Z984 U6CV53 G9K4T4 L5L5C4 G1PUZ0 A0A2R8M6R9 A0A2Y9KWY9 G1T125 H2P3X7 C1BWA0 A0A2I2U3M8 M3YQ49 G1QPI3 C4A116 A0A096NRI2 A0A384NYJ4 Q8IWL3 A0A3Q0T5R0 A0A402FFF3 A0A1B6HTT0 A0A1B6IWU9 A0A0T6B6W2 A0A3P9KNQ1 A0A3B5BA12 L8IZL5 F1MLK9 A0A0P6J7F1 A6QLM8 A0A1B6KGD9 A0A341D9Q8 W5NXH4 F6UZ83 A0A452G4V6 A0A3Q7S991 A0A2T7NFC4 A0A1S3JYJ6 G5APY5 A0A3P9M4V1 A0A3B3HNZ8

PDB

3BVO E-value=1.96384e-22, Score=256

KEGG

101745431 no KO assigned | (RefSeq) retrovirus-related Pol polyprotein from transposon 17.6

GO

GO:0051087 GO:0051259 GO:0097428 GO:0001671 GO:0004252 GO:0005634 GO:0005829 GO:0005739 GO:0042802 GO:0016226 GO:0044571 GO:0005737 GO:0046872 GO:0006457 GO:0008449 GO:0030203 GO:0003723 GO:0006730 GO:0009258 GO:0016155 GO:0003824

PANTHER

PTHR14021

Subcellular location

Cytoplasm
Mitochondrion

Length:

161

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0

Exp number, first 60 AAs:

0

Total prob of N-in:

0.14249

outside

1 - 161

Pi

393.380714

Theta

218.615478

Tajima's D

2.345367

CLR

0

CSRT

0.930153492325384

Interpretation

Uncertain

Sequence

Summary

Ontologies

Topology

Population Genetic Test Statistics