Silkworm Genome Informatics Database

Gene

KWMTBOMO09654 Validated by peptides from experiments

Pre Gene Modal

BGIBMGA008046

Annotation

PREDICTED:_vacuolar_protein_sorting-associated_protein_33A-like_[Bombyx_mori]

Location

Bomo_Chr16(+):6319458-6332822 View in EpiBrowser

Full name

Vacuolar protein sorting-associated protein 33A

Location in the cell

Nuclear Reliability : 2.461

CDS

ATGAGAGAAACTAAAATGAGCAGCCACCTTGCGGGTGGACGACTGAATGTTGCTTTACTTCAAGAAACAATGAGAACAGAATTATTAAATTTATTACAACAGTACAGTGGCCCTAAAGTAACAATTTGGGATGACTGGCTAGCTGGGCCTGTAGGCCTTGTTGCTCAGTACTCATTCCTTAAAGACCATGAAGTCAACAATATGTTTCTTCTGAAACCAGGAAGCCTACCCAATATAACCGTCAAACATATTATATTTATTAGTAGACCCAAACTTTCATTGATGGATTTGGTTGCTGATTACATTTTATCAATAAGGGCCAAGCAAACAGCTCCAGTAGAATTTCACTTGTTCTTTGTGCCACGCAAAAGTGAACTGTGCAAAGTACATCTCACCAATAGAGGAGTGGTCAGTAACATGACAATTGAAGAGTTTAAGTGTGACATCATTCCATTCGAAAGTGATGTCATGTCTTTAGAACTACAAAATGACTTCCGAGAAAATTACATAGAAGGTGACACAAGTTGCATATACAATGCTGCACAGGCACTAAGAACCATCCAACAGTTTTATGGAATTGTGCCTCGTGTATTCGGGAAAGGCCAAGCAGCTAAACAGGTTTGGGATCTATTGTGCAGATTGAATAAAGAGGAGCAAGGTTCCGGGAATAAGGGTGCACCGACATCATGCATCGATCAGTTACTCTTATTGGACCGAGCCGTTGACTTTACAAGTGTAATGGCGACCCAACTCACCTATGAGGGGCTTATTGACGAAATCTACCGCATAAAGAGCGGTACGGCCACGTTTCCGGGACAAAAGTTCGTGGGTCCGGACGATCCTAACCCAGAAGAGACAGCCCGTGAGAAGAAACACGTCATACTCAACTCCAGCGAAGAACTGTTTGCAGAAATACGCGACTCTGACTTTACCGAGATAGGAGCAGCATTATCGAAAAAGGCTCGCATAATTAAAACACAACTAGATGAGCGCCACAAAGACAAATCCGTGCAAGAGATCAAGCATTTTGTTTCCCGCCTACCGCAGCTGCTGGCCAACAAGCAGTCCCTGGTCACTCACACCGCCATCGCTGAGTACATCAAAGAGAAAACTGACAGCTTCGATTTTCATGACAAAATACAATGCGAAGAAGACTTTCTGAATTGTATCGACAGCGAAAAGATTTGTCCGTATATTGAAGACCTGATTGCACAGAAGGAACCTCTTACCAAGGTTCTGCGCTTGATTTGCCTACAATGCGCAACCGGGTCTGGCTTGAAGCCGAAAATTCTGGAATATTACAAGAGGGAACTCGTGCAAACGTACGGTCTGAAGACCTGGCTAAGTATCTGCAACTTGGAGAAGTGCGGCCTGCTCAAAACACAACAAGGAACCCGTCAATATACTGTACTCAGAAAGGCCTTACATCTGACGACTGACGATTCCGATGCAAATGACAAAGACAAAAGTTATTTGTCGAGTAAATATACACCGTTAACAGTGAGACTGAGCGAGCATATCGCCAAGAATAAAGGATGGAGCGGTATCCAAGACGTATTAGGCCTCCTGCCGGGCCCTACAGTGGACGAGCTACAAACCCTACAACCTCGTATGACACGTCGCCATTCCGGTTCCAGTGAGAATTCATCATCAATAGAAAACCCACGGGTCGTGCTCGTATTCTTCATTGGAGGCTGTACGTACCACGAGATCTCAGTGCTCAGGACGATCAGCCAGCAGGAAGATTCGGTTGTGGAATTTGTTATCTTAACCACTAAAATAATCAATGGAACCACATTCATAGAATCTTTAATGGAACTCGATTGA

Protein

MRETKMSSHLAGGRLNVALLQETMRTELLNLLQQYSGPKVTIWDDWLAGPVGLVAQYSFLKDHEVNNMFLLKPGSLPNITVKHIIFISRPKLSLMDLVADYILSIRAKQTAPVEFHLFFVPRKSELCKVHLTNRGVVSNMTIEEFKCDIIPFESDVMSLELQNDFRENYIEGDTSCIYNAAQALRTIQQFYGIVPRVFGKGQAAKQVWDLLCRLNKEEQGSGNKGAPTSCIDQLLLLDRAVDFTSVMATQLTYEGLIDEIYRIKSGTATFPGQKFVGPDDPNPEETAREKKHVILNSSEELFAEIRDSDFTEIGAALSKKARIIKTQLDERHKDKSVQEIKHFVSRLPQLLANKQSLVTHTAIAEYIKEKTDSFDFHDKIQCEEDFLNCIDSEKICPYIEDLIAQKEPLTKVLRLICLQCATGSGLKPKILEYYKRELVQTYGLKTWLSICNLEKCGLLKTQQGTRQYTVLRKALHLTTDDSDANDKDKSYLSSKYTPLTVRLSEHIAKNKGWSGIQDVLGLLPGPTVDELQTLQPRMTRRHSGSSENSSSIENPRVVLVFFIGGCTYHEISVLRTISQQEDSVVEFVILTTKIINGTTFIESLMELD

Description

Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act as a core component of the putative HOPS and CORVET endosomal tethering complexes which are proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The HOPS complex is proposed to be recruited to Rab7 on the late endosomal membrane and to regulate late endocytic, phagocytic and autophagic traffic towards lysosomes. The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations. Required for fusion of endosomes and autophagosomes with lysosomes; the function is dependent on its association with VPS16 but not VIPAS39. The function in autophagosome-lysosome fusion implicates STX17 but not UVRAG.

Subunit

Core component of at least two putative endosomal tethering complexes, the homotypic fusion and vacuole protein sorting (HOPS) complex and the class C core vacuole/endosome tethering (CORVET) complex. Their common core is composed of the class C Vps proteins VPS11, VPS16, VPS18 and VPS33A, which in HOPS further associates with VPS39 and VPS41 and in CORVET with VPS8 and TGFBRAP1 (PubMed:11382755). Interacts with RAB5C, UVRAG, STX17, MON1A and MON1B. Associates with adaptor protein complex 3 (AP-3) and clathrin (By similarity).

Similarity

Belongs to the STXBP/unc-18/SEC1 family.

Keywords

Complete proteome Cytoplasmic vesicle Endosome Lysosome Membrane Protein transport Reference proteome Transport

Feature

chain Vacuolar protein sorting-associated protein 33A

Uniprot

A0A2A4JXK5 A0A2H1VMW5 A0A3S2NH69 A0A194Q020 A0A212FMF2 H9JEU9 + More
A0A194R2R0 A0A2A4JXW9 A0A1E1WG75 A0A067QIC5 A0A1Y1L4Y8 D6WYR9 A0A151JUA5 A0A151I575 A0A158NX93 A0A195EDG0 E9IVX0 A0A151I6P3 A0A087ZVQ0 K7J4K3 A0A151WYJ2 E2ANA6 A0A2A3E920 A0A154PJD3 A0A0C9PWJ5 A0A026WDH2 A0A1B6KWM8 A0A1W4XPN8 A0A310SEW7 N6TZH8 A0A0J7KYZ6 A0A232F1D4 A0A023F2W6 A0A1L8DVZ6 A0A0P4VYT5 A0A069DZC0 A0A1B0CP49 A0A1B6E3U9 A0A0N0U5X1 E0W3Q6 A0A224XKL3 A0A087U8M3 V9IM27 A0A182F6U6 A0A2M4AIM6 A0A0C9QQ98 B0X2Y2 F7CE04 W5JJG4 A0A0S7FZL1 A0A336LM58 A0A182N5T7 G3W582 L5KU03 Q17Q10 C0H961 H0WTA8 A0A3Q2ZQC0 I3MNU1 A0A060XHW6 W5MDK1 A0A218V5P7 A0A3Q2WPW3 A0A3L8SCR9 M3ZIG1 A0A3P8PF95 A0A3P9BX88 A0A3Q0D7S5 A0A2M4AI45 A0A091G403 A0A093HEA2 V9KFL4 A0A146P4L9 A0A182J3I1 A0A2U9BRX2 W5L2L9 A0A1S3H0H1 A0A2I4BIG9 G3H267 H0VDU1 V4A284 A0A3B4GMH9 A0A1A8FH75 A0A3Q7QI85 A0A2U3VLR5 A0A146NKT8 A0A3B3Z0G8 A0A091HL02 A0A1A8AQI2 Q3KRF0 M3WQ76 W5PPJ2 A0A1U7TY01 Q63615 A0A3P8WFT1 A0A087Y9S2 A0A3B3VCF9 A0A452TFP4 G1SH85 A0A452EZQ4

Pubmed

26354079 22118469 19121390 24845553 28004739 18362917 + More
19820115 21347285 21282665 20075255 20798317 24508170 23537049 28648823 25474469 27129103 26334808 20566863 17495919 20920257 23761445 21709235 23258410 17510324 20433749 24755649 30282656 23542700 25186727 26319212 28071753 24402279 25329095 21804562 21993624 23254933 15489334 15632090 17975172 20809919 8996080 11382755 24487278 24813606

EMBL

NWSH01000463 PCG76233.1 ODYU01003407 SOQ42136.1 RSAL01000102 RVE47456.1 + More
KQ459582 KPI98911.1 AGBW02007651 OWR54944.1 BABH01023336 KQ460878 KPJ11535.1 PCG76232.1 GDQN01005138 JAT85916.1 KK853322 KDR08539.1 GEZM01066460 GEZM01066459 GEZM01066458 GEZM01066457 JAV67868.1 KQ971357 EFA08455.1 KQ981799 KYN35664.1 KQ976434 KYM87225.1 ADTU01003012 ADTU01003013 KQ979074 KYN22884.1 GL766449 EFZ15191.1 KQ978464 KYM93774.1 AAZX01002348 AAZX01012179 KQ982651 KYQ52856.1 GL441177 EFN65054.1 KZ288327 PBC27964.1 KQ434924 KZC11574.1 GBYB01005868 JAG75635.1 KK107260 EZA54018.1 GEBQ01024343 JAT15634.1 KQ769895 OAD52769.1 APGK01055308 KB741261 ENN71657.1 LBMM01001926 KMQ95523.1 NNAY01001324 OXU24363.1 GBBI01003261 JAC15451.1 GFDF01003495 JAV10589.1 GDKW01000818 JAI55777.1 GBGD01000905 JAC87984.1 AJWK01021424 GEDC01004713 JAS32585.1 KQ435750 KOX76251.1 DS235882 EEB20262.1 GFTR01007284 JAW09142.1 KK118731 KFM73712.1 JR051095 AEY61429.1 GGFK01007137 MBW40458.1 GBYB01005869 JAG75636.1 DS232303 EDS39484.1 ADMH02001255 ETN63433.1 GBYX01460220 GBYX01460209 JAO21355.1 UFQT01000045 SSX18805.1 AEFK01071562 AEFK01071563 AEFK01071564 KB030576 ELK14303.1 CH477188 EAT48784.1 BT058867 ACN10580.1 AAQR03124472 AAQR03124473 AAQR03124474 AAQR03124475 AGTP01058503 AGTP01058504 FR905184 CDQ76904.1 AHAT01005128 MUZQ01000045 OWK61158.1 QUSF01000030 RLV99826.1 GGFK01007129 MBW40450.1 KL447648 KFO75799.1 KL217402 KFV77312.1 JW864063 AFO96580.1 GCES01147409 JAQ38913.1 CP026251 AWP06801.1 JH000113 EGW07482.1 AAKN02052334 KB201304 ESO97948.1 HAEB01011646 HAEC01002260 SBQ58173.1 GCES01154270 JAQ32052.1 KL217497 KFO95792.1 HADY01018271 SBP56756.1 BC105752 CH473973 AAI05753.1 EDM13629.1 AANG04002472 AMGL01039208 AMGL01039209 U35244 AYCK01000969 LWLT01000018

Proteomes

UP000218220 UP000283053 UP000053268 UP000007151 UP000005204 UP000053240 + More
UP000027135 UP000007266 UP000078541 UP000078540 UP000005205 UP000078492 UP000078542 UP000005203 UP000002358 UP000075809 UP000000311 UP000242457 UP000076502 UP000053097 UP000192223 UP000019118 UP000036403 UP000215335 UP000092461 UP000053105 UP000009046 UP000054359 UP000069272 UP000002320 UP000002280 UP000000673 UP000075884 UP000007648 UP000010552 UP000008820 UP000087266 UP000005225 UP000264800 UP000005215 UP000193380 UP000018468 UP000197619 UP000264840 UP000276834 UP000002852 UP000265100 UP000265160 UP000189706 UP000053760 UP000053875 UP000265000 UP000075880 UP000246464 UP000018467 UP000085678 UP000192220 UP000001075 UP000005447 UP000030746 UP000261460 UP000286641 UP000245340 UP000261480 UP000054308 UP000011712 UP000002356 UP000189704 UP000002494 UP000265120 UP000028760 UP000261500 UP000291021 UP000001811 UP000291000

PRIDE

H9JEU9 G3H267 Q63615

Pfam

PF00995   Sec1        + More
PF00069   Pkinase
PF00566   RabGAP-TBC

Interpro

IPR027482   Sec-1-like_dom2        + More
IPR027121   VPS33
IPR001619   Sec1-like
IPR036045   Sec1-like_sf
IPR000195   Rab-GTPase-TBC_dom
IPR036873   Rhodanese-like_dom_sf
IPR000719   Prot_kinase_dom
IPR011009   Kinase-like_dom_sf
IPR035969   Rab-GTPase_TBC_sf

SUPFAM

SSF56815   SSF56815        + More
SSF47923   SSF47923
SSF56112   SSF56112
SSF52821   SSF52821

Gene 3D

3.40.50.1910 3.40.250.10

ProteinModelPortal

A0A2A4JXK5 A0A2H1VMW5 A0A3S2NH69 A0A194Q020 A0A212FMF2 H9JEU9 + More
A0A194R2R0 A0A2A4JXW9 A0A1E1WG75 A0A067QIC5 A0A1Y1L4Y8 D6WYR9 A0A151JUA5 A0A151I575 A0A158NX93 A0A195EDG0 E9IVX0 A0A151I6P3 A0A087ZVQ0 K7J4K3 A0A151WYJ2 E2ANA6 A0A2A3E920 A0A154PJD3 A0A0C9PWJ5 A0A026WDH2 A0A1B6KWM8 A0A1W4XPN8 A0A310SEW7 N6TZH8 A0A0J7KYZ6 A0A232F1D4 A0A023F2W6 A0A1L8DVZ6 A0A0P4VYT5 A0A069DZC0 A0A1B0CP49 A0A1B6E3U9 A0A0N0U5X1 E0W3Q6 A0A224XKL3 A0A087U8M3 V9IM27 A0A182F6U6 A0A2M4AIM6 A0A0C9QQ98 B0X2Y2 F7CE04 W5JJG4 A0A0S7FZL1 A0A336LM58 A0A182N5T7 G3W582 L5KU03 Q17Q10 C0H961 H0WTA8 A0A3Q2ZQC0 I3MNU1 A0A060XHW6 W5MDK1 A0A218V5P7 A0A3Q2WPW3 A0A3L8SCR9 M3ZIG1 A0A3P8PF95 A0A3P9BX88 A0A3Q0D7S5 A0A2M4AI45 A0A091G403 A0A093HEA2 V9KFL4 A0A146P4L9 A0A182J3I1 A0A2U9BRX2 W5L2L9 A0A1S3H0H1 A0A2I4BIG9 G3H267 H0VDU1 V4A284 A0A3B4GMH9 A0A1A8FH75 A0A3Q7QI85 A0A2U3VLR5 A0A146NKT8 A0A3B3Z0G8 A0A091HL02 A0A1A8AQI2 Q3KRF0 M3WQ76 W5PPJ2 A0A1U7TY01 Q63615 A0A3P8WFT1 A0A087Y9S2 A0A3B3VCF9 A0A452TFP4 G1SH85 A0A452EZQ4

PDB

4BX9 E-value=1.05403e-155, Score=1413

KEGG

101738893 K20182 vacuolar protein sorting-associated protein 33A | (RefSeq) vacuolar protein sorting-associate

GO

GO:0006904 GO:0005524 GO:0004672 GO:0005776 GO:0005764 GO:0033263 GO:0043473 GO:0048070 GO:0030220 GO:0035751 GO:0005769 GO:0030136 GO:0031410 GO:0044877 GO:0031902 GO:0005765 GO:0015031 GO:0016192 GO:0008270 GO:0006413 GO:0031369 GO:0003723 GO:0006730 GO:0009258 GO:0016155 GO:0003824

PANTHER

PTHR11679:SF56 PTHR11679

Subcellular location

Cytoplasmic vesicle
Late endosome membrane
Lysosome membrane
Early endosome
Autophagosome
Clathrin-coated vesicle

Length:

608

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.0835800000000002

Exp number, first 60 AAs:

0.00586

Total prob of N-in:

0.00335

outside

1 - 608

Pi

228.83282

Theta

199.130989

Tajima's D

0.540176

CLR

0.020204

CSRT

0.528823558822059

Interpretation

Possibly Positive selection

Sequence

Summary

Ontologies

Topology

Population Genetic Test Statistics