Gene

KWMTBOMO09355  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA002186

Annotation

thiol_peroxiredoxin_[Bombyx_mori]

Location

Bomo_Chr15(+):17860955-17877543      View in EpiBrowser

Full name

Peroxiredoxin 1      

Alternative Name

Cytosolic thioredoxin peroxidase
Thioredoxin peroxidase

Location in the cell

Cytoplasmic   Reliability : 2.647

CDS

ATGCCTCTGCAGATGACCAAACCCGCTCCCCAGTTCAAGGCCACGGCCGTCGTCAACGGAGAGTTCAAGGACATTTCTCTGTCTGACTACAAGGGGAAATATGTTGTGCTGTTCTTCTATCCTTTGGACTTCACGTTCGTGTGCCCGACGGAGATCATCGCGTTCTCGGAGAAGGCGGACGAGTTCCGCAAGATCGGCTGCGAGGTGCTCGGCGCCTCCACCGACTCGCACTTCACGCACCTCGCCTGGATCAACACGCCGCGCAAGCAGGGCGGACTCGGTCCCATGAACATCCCTCTGATAAGCGACAAGTCGCACCGCATCTCCCGCGACTACGGAGTGCTGGACGAGGAGACGGGCATCCCCTTCCGAGGACTCTTCATCATCGACGACAAGCAGAACCTCAGGCAGATCACCATCAACGACCTGCCCGTGGGGAGGTCGGTGGAGGAGACCCTGCGGCTGGTGCAGGCGTTCCAGTTCACGGACAAGCACGGCGAGGTGTGCCCCGCCAACTGGAGGCCCGGCGCCAAGACCATCAAGCCCGACACCAAGGCCGCGCAGGAGTACTTCGGCGACGCCAACTAG

Protein

MPLQMTKPAPQFKATAVVNGEFKDISLSDYKGKYVVLFFYPLDFTFVCPTEIIAFSEKADEFRKIGCEVLGASTDSHFTHLAWINTPRKQGGLGPMNIPLISDKSHRISRDYGVLDEETGIPFRGLFIIDDKQNLRQITINDLPVGRSVEETLRLVQAFQFTDKHGEVCPANWRPGAKTIKPDTKAAQEYFGDAN

Description

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2) (By similarity). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation (By similarity).

Catalytic Activity

[protein]-dithiol + a hydroperoxide = [protein]-disulfide + an alcohol + H2O

Subunit

Homodimer; disulfide-linked, upon oxidation. 5 homodimers assemble to form a ring-like decamer (By similarity). Interacts with GDPD5; forms a mixed-disulfide with GDPD5 (By similarity). Interacts with SESN1 and SESN2 (By similarity).

Miscellaneous

The active site is a conserved redox-active cysteine residue, the peroxidatic cysteine (C(P)), which makes the nucleophilic attack on the peroxide substrate. The peroxide oxidizes the C(P)-SH to cysteine sulfenic acid (C(P)-SOH), which then reacts with another cysteine residue, the resolving cysteine (C(R)), to form a disulfide bridge. The disulfide is subsequently reduced by an appropriate electron donor to complete the catalytic cycle. In this typical 2-Cys peroxiredoxin, C(R) is provided by the other dimeric subunit to form an intersubunit disulfide. The disulfide is subsequently reduced by thioredoxin (By similarity). As a reducing substrate, thioredoxin 2 is preferred over thioredoxin 1 (PubMed:11877442).

Similarity

Belongs to the peroxiredoxin family. AhpC/Prx1 subfamily.

Keywords

Antioxidant   Complete proteome   Cytoplasm   Disulfide bond   Oxidoreductase   Peroxidase   Phosphoprotein   Redox-active center   Reference proteome   Ubl conjugation  

Feature

chain  Peroxiredoxin 1

Uniprot

Q6T3A7   A0A088MGF5   A0A2S0RQQ1   A0A2L1CF95   A0A2A4IX67   A0A212EPQ2   + More
B2KSE9   W0G2G4   A0A194QN77   A0A194Q0R0   I4DJ66   A0A3G1T1I6   A0A310SIG5   A0A0L7R4M0   A0A087ZND2   A0A154PDX5   E2BSB2   C3VVL4   A0A3L8DIR4   A0A026WMD6   A0A2A3E783   V9IJ36   T1P9R6   A0A0N0BCN5   A0A0L0C893   A0A1W4VJ93   A0A0M3QZ91   E2ALM7   B3MXP7   B4R4B3   B4IGR0   X2JF59   Q9V3P0   A0A067QT52   G0WRL1   A0A411G805   A0A195DBS0   Q6XJ14   B3NWP9   A0A151I178   A0A158NMR0   A0A3B0JT65   L0ATY2   A0A151X141   A0A151IBS9   A0A0R3P480   Q29IR1   B4H2V8   A0A2J7QKP8   A0A232ET67   A0A0C9QZH8   T1E316   B4NBT6   F4WVS6   A0A1L8EF12   A0A0Q9X143   A0A151JTE8   B4L6U4   A0A023EJ61   K7J7Q6   A0A2P8Z9A3   A0A0J7L7W7   A0A0C9QCP4   A0A0K8TWN4   A0A034W382   A0A1L7LQE0   B1N694   U5EZ75   W8BQT7   A0A1I8Q0P3   Q17DN4   Q8WSF6   V5K6U7   A2I476   A0A0K8TUD5   A0A3P8ZAF2   A0A0A1WZP3   B4MA19   A0A182T5T8   A0A0F6MVY1   A0A182JGY2   A0A0B6Z6H1   A0A1L8EEU6   A0A0B6Z732   A0A3P9AQB1   A0A0B6Z4Q3   A0A3N0YJY2   I3PU24   T1DN15   A0A146J914   A0A2I8B5Y1   A0A1B6F8I1   A0A1W4WZJ8   A0A1B6INE9   B5XBY3   A0A1D2AJ30   W5LIA3   C1KC72   B5X7X7   A0A0M3SH29  

EC Number

1.11.1.15

Pubmed

15607657    19121390    25191834    22118469    24296440    26354079    + More
22651552    20798317    19808099    19932185    30249741    24508170    25315136    26108605    17994087    10731132    12537568    12537572    12537573    12537574    16110336    17569856    17569867    26109357    26109356    10632718    11677042    12537569    11877442    18327897    26715182    28953965    24845553    21382109    14525923    17550304    21347285    15632085    28648823    24330624    21719571    18057021    24945155    26483478    20075255    29403074    25348373    26760975    28076409    18226547    24495485    17510324    24420863    26369729    25069045    25830018    20433749    25329095    22450240   

EMBL

BABH01029814    AY438331    AAR15420.1    KJ995804    AIN39487.1    MF979106    + More
AWA45969.1    MF521978    AVC05622.1    NWSH01005971    PCG63782.1    AGBW02013414    OWR43468.1    EU131680    ABW96360.1    KC685523    KC685524    AHF48621.1    KQ461195    KPJ06809.1    KQ459581    KPI99141.1    AK401334    BAM17956.1    MG992413    AXY94851.1    KQ760761    OAD59133.1    KQ414657    KOC65769.1    KQ434885    KZC10041.1    GL450157    EFN81425.1    FJ895866    FJ895867    ACP44066.1    QOIP01000007    RLU20033.1    KK107152    EZA57227.1    KZ288357    PBC27142.1    JR047795    AEY60511.1    KA645439    AFP60068.1    KQ435897    KOX69212.1    JRES01000755    KNC28658.1    CP012528    ALC49010.1    ALC49013.1    GL440609    EFN65636.1    CH902630    EDV38512.1    CM000366    EDX17815.1    CH480836    EDW49010.1    AE014298    AHN59672.1    AHN59673.1    AHN59674.1    AF167098    AF321615    AF321616    AY070534    BT014630    KK853308    KDR08683.1    HM641254    HM641255    ADT65135.1    MH366217    QBB02026.1    KQ981010    KYN10321.1    AY231665    CM000162    AAR09688.1    EDX01561.1    CH954180    EDV47211.1    KQ976593    KYM79656.1    ADTU01020675    OUUW01000003    SPP78650.1    JX915909    AFZ78684.1    KQ982588    KYQ54117.1    KQ978078    KYM97181.1    CH379063    KRT06147.1    EAL32592.3    CH479204    EDW30675.1    NEVH01013260    PNF29153.1    NNAY01002315    OXU21560.1    GBYB01001115    GBYB01010548    JAG70882.1    JAG80315.1    GALA01000785    JAA94067.1    CH964239    EDW82295.1    GL888394    EGI61696.1    GFDG01001521    JAV17278.1    KRF99593.1    KQ981936    KYN32759.1    CH933812    EDW06090.1    KRG07143.1    JXUM01009055    GAPW01004617    KQ560275    JAC08981.1    KXJ83372.1    AAZX01009307    PYGN01000140    PSN53079.1    LBMM01000362    KMQ99389.1    GBYB01001084    JAG70851.1    GDHF01033829    GDHF01023805    GDHF01007797    JAI18485.1    JAI28509.1    JAI44517.1    GAKP01010201    GAKP01010200    JAC48752.1    FX983166    BAV93784.1    EF103377    ABO26635.1    GANO01001301    JAB58570.1    GAMC01002925    JAC03631.1    CH477292    EAT44541.2    AF323181    AAL37254.1    KC197034    AGU16435.1    EF070579    ABM55645.1    GDAI01000053    JAI17550.1    GBXI01013087    GBXI01010287    JAD01205.1    JAD04005.1    CH940655    EDW66078.1    JX021298    AGG68945.1    HACG01016621    CEK63486.1    GFDG01001542    JAV17257.1    HACG01016620    HACG01016622    CEK63485.1    CEK63487.1    HACG01016623    CEK63488.1    RJVU01037554    ROL46546.1    JF795590    AFG25786.2    GAMD01003255    JAA98335.1    LC049075    BAU79645.1    MF346707    AUT13197.1    GECZ01023546    JAS46223.1    GECU01019261    JAS88445.1    BT048552    BT057679    BT149987    ACI68353.1    ACM09551.1    AGH92591.1    GETE01000212    JAT79209.1    FJ812091    JX133230    MF319750    ACO36036.1    AFR11898.1    ASS34529.1    BT047146    ACI66947.1    KP192122    ALD62538.1   

Proteomes

UP000005204    UP000218220    UP000007151    UP000053240    UP000053268    UP000053825    + More
UP000005203    UP000076502    UP000008237    UP000279307    UP000053097    UP000242457    UP000095301    UP000053105    UP000037069    UP000192221    UP000092553    UP000000311    UP000007801    UP000000304    UP000001292    UP000000803    UP000027135    UP000078492    UP000002282    UP000008711    UP000078540    UP000005205    UP000268350    UP000075809    UP000078542    UP000001819    UP000008744    UP000235965    UP000215335    UP000007798    UP000007755    UP000078541    UP000009192    UP000069940    UP000249989    UP000002358    UP000245037    UP000036403    UP000095300    UP000008820    UP000265140    UP000008792    UP000075901    UP000075880    UP000192223    UP000087266    UP000018467   

PRIDE

Q6T3A7    B2KSE9    B3MXP7    X2JF59    Q9V3P0    Q6XJ14    + More
B3NWP9   

Pfam

PF10417   1-cysPrx_C        + More
PF00578   AhpC-TSA

Interpro

IPR019479   Peroxiredoxin_C        + More
IPR013766   Thioredoxin_domain       
IPR036249   Thioredoxin-like_sf       
IPR024706   Peroxiredoxin_AhpC-typ       
IPR000866   AhpC/TSA       

SUPFAM

SSF52833   SSF52833       

ProteinModelPortal

Q6T3A7   A0A088MGF5   A0A2S0RQQ1   A0A2L1CF95   A0A2A4IX67   A0A212EPQ2   + More
B2KSE9   W0G2G4   A0A194QN77   A0A194Q0R0   I4DJ66   A0A3G1T1I6   A0A310SIG5   A0A0L7R4M0   A0A087ZND2   A0A154PDX5   E2BSB2   C3VVL4   A0A3L8DIR4   A0A026WMD6   A0A2A3E783   V9IJ36   T1P9R6   A0A0N0BCN5   A0A0L0C893   A0A1W4VJ93   A0A0M3QZ91   E2ALM7   B3MXP7   B4R4B3   B4IGR0   X2JF59   Q9V3P0   A0A067QT52   G0WRL1   A0A411G805   A0A195DBS0   Q6XJ14   B3NWP9   A0A151I178   A0A158NMR0   A0A3B0JT65   L0ATY2   A0A151X141   A0A151IBS9   A0A0R3P480   Q29IR1   B4H2V8   A0A2J7QKP8   A0A232ET67   A0A0C9QZH8   T1E316   B4NBT6   F4WVS6   A0A1L8EF12   A0A0Q9X143   A0A151JTE8   B4L6U4   A0A023EJ61   K7J7Q6   A0A2P8Z9A3   A0A0J7L7W7   A0A0C9QCP4   A0A0K8TWN4   A0A034W382   A0A1L7LQE0   B1N694   U5EZ75   W8BQT7   A0A1I8Q0P3   Q17DN4   Q8WSF6   V5K6U7   A2I476   A0A0K8TUD5   A0A3P8ZAF2   A0A0A1WZP3   B4MA19   A0A182T5T8   A0A0F6MVY1   A0A182JGY2   A0A0B6Z6H1   A0A1L8EEU6   A0A0B6Z732   A0A3P9AQB1   A0A0B6Z4Q3   A0A3N0YJY2   I3PU24   T1DN15   A0A146J914   A0A2I8B5Y1   A0A1B6F8I1   A0A1W4WZJ8   A0A1B6INE9   B5XBY3   A0A1D2AJ30   W5LIA3   C1KC72   B5X7X7   A0A0M3SH29  

PDB

4XCS     E-value=1.80199e-84,     Score=792

KEGG

692638    K13279 peroxiredoxin 1 [EC:1.11.1.15] | (RefSeq) Jafrac1; thiol peroxiredoxin

PATHWAY

04146    Peroxisome - Bombyx mori (domestic silkworm)

GO

GO:0045454   GO:0005623   GO:0051920   GO:0004601   GO:0005829   GO:0042594   GO:0008354   GO:0007155   GO:0007281   GO:0008379   GO:0006979   GO:0042744   GO:0008340   GO:0016209   GO:0016491   GO:0005200   GO:0003676   GO:0004812   GO:0006418   GO:0000049   GO:0016876   GO:0055114  

Subcellular location

Cytoplasm  

Length:

195

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

4.81683

Exp number, first 60 AAs:

4.81482

Total prob of N-in:

0.11698

outside

1  -  195

 

 

Pi

348.779899

Theta

200.214408

Tajima's D

2.419239

CLR

0.464569

CSRT

0.936853157342133

Interpretation

Uncertain