Gene

KWMTBOMO08888

Annotation

putative_lipoate-protein_ligase_b_[Danaus_plexippus]

Location

Bomo_Chr15(+):7465590-7467900      View in EpiBrowser

Full name

Putative lipoyltransferase 2, mitochondrial      

Alternative Name

Lipoate-protein ligase B
Lipoyl/octanoyl transferase
Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Location in the cell

Cytoplasmic   Reliability : 1.84 Mitochondrial   Reliability : 1.86

CDS

ATGGTTAAAATTTGGAGGCTGGGTTTAATGAGCTATGATACTGCTCTTAATATCCAATTAGCTATAGCACGAAAACATCTCGATTCAATTAAGAATGGCCTGGATTTTAATCATGATACCCTTTTACTTGTAGAACATAAACCTGTTTATACAGTAGGTATAAGAGACAAGACACCAAACAATGAAATTCTCAGGCTAAAAAGTCTGGGAGCAGATTTTAAGAAAACAAACAGAGGCGGTCTTGTAACTTTTCATGGGCCTGGTCAATTAGTTGCATACCCAATTATAAATTTGAAGTACTATAAACCAAGTGTGAAGTGGTATGTAAATAGTTTGGAAGAAATTGGCATAAGATTGTGTGGTGACTTAGGAATTCAAGCCAGCAGATCACCTCACACAGGAGTATGGGTGGCAGATAACAAAATAGCCGCAATAGGTGTTCACACCTCGAGATATGTCACAACCCATGGTATTGCAATAAATTGTGATATTGACCTTTCTTGGTTCAAACACATAGACCCCTGTGGTATAGTTGACAAAGGAGTTACATCATTGACCAAAGAAACAGGAGATTTGTGCACAATAGACAAAATAACACCATTGTTTTTGAGTAAATTTCAAGATGTGTTTGAATGTGAGATAGAAGACCTGGACTTGAAGTCCCAAAAAGATATTCTGAGTTATGTATATAATAAATTAATGAAGGAATTGATGGTACAAAATACAGCATAA

Protein

MVKIWRLGLMSYDTALNIQLAIARKHLDSIKNGLDFNHDTLLLVEHKPVYTVGIRDKTPNNEILRLKSLGADFKKTNRGGLVTFHGPGQLVAYPIINLKYYKPSVKWYVNSLEEIGIRLCGDLGIQASRSPHTGVWVADNKIAAIGVHTSRYVTTHGIAINCDIDLSWFKHIDPCGIVDKGVTSLTKETGDLCTIDKITPLFLSKFQDVFECEIEDLDLKSQKDILSYVYNKLMKELMVQNTA

Description

Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate.
Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes, which catalyze essential redox reactions (By similarity). Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate (By similarity).

Catalytic Activity

L-lysyl-[protein] + octanoyl-[ACP] = H(+) + holo-[ACP] + N(6)-octanoyl-L-lysyl-[protein]

Miscellaneous

In the reaction, the free carboxyl group of octanoic acid is attached via an amide linkage to the epsilon-amino group of a specific lysine residue of lipoyl domains of lipoate-dependent enzymes.

Similarity

Belongs to the LipB family.

Keywords

Acyltransferase   Complete proteome   Ligase   Mitochondrion   Reference proteome   Transferase   Transit peptide  

Feature

chain  Putative lipoyltransferase 2, mitochondrial

Uniprot

A0A212FKU4   A0A2A4IZ19   A0A2H1WEA6   A0A194RE29   A0A194PKI5   A0A437BVD1   + More
A0A0L7LAW1   A0A2J7QYL2   A0A0L7QY58   E2A2V5   A0A2P8XZI4   A0A3P8YKA1   A0A2T7NCM6   A0A088ALW7   A0A151I3F1   A0A2A3E6B0   A0A154NWE4   C1BYA8   A0A158NXY6   U4U015   H3A1P6   A0A1W4YTY4   Q0IEK7   A0A0J7L363   N6T2D6   A0A060WM11   A0A3B1KFN2   A0A0P7UV33   E3TGJ5   F4WMW4   A0A067RKW3   A0A3B3CEV7   A0A3B1JQ22   A0A1S4FN42   A0A151WIG7   A0A3B4DS71   A0A151IVW7   A0A2D0SSE5   A0A0P6FSE3   A0A3N0XI49   E2BQH3   A0A0P5KBG0   A0A1B6LDI8   A0A0P6BGB1   A0A1A9UZU6   A0A0M9A1L5   E9IID1   A0A2R7WBM1   B0WH35   A0A1S3NPT8   D6WCX6   K7IYB8   U5EXR5   W4XML7   A0A1B0AL12   A0A1A9XN22   A0A1B0FR83   A0A2C9JF27   A0A1B6HLI8   A0A3Q1ETW8   A0A437CTR0   A0A0L8FR25   A0A1A8P1U8   Q4SB31   A0A3Q1D0S5   A0A3P8TSF6   A0A182LXS1   A0A1A9ZZQ8   A0A026VU07   A0A1A8J639   A0A1A8C774   A0A1A7ZW42   A0A1B0CL88   A0A131XKJ9   A0A3B5B6N9   E0VS59   A0A1A8H0Q2   A0A1L8HBD3   A0A3Q4N1Z4   I3KKT0   A0A3M6UTA1   A0A0F8BU33   Q7PS31   A0A182XEE9   A0A182IAB4   Q29R99   R7V6C9   A0A1Q3EXD3   A0A1A8L678   A0A2M4CS08   A0A3B3QZL2   A0A3Q1J525   A0A1S3DW12   A0A1L8E0L8   F1QAW5   A0A131YV83   A0A182S5L7   A0A3Q2R0K8   A0A3P9MXY6   A0A3B4ZA36  

EC Number

2.3.1.181

Pubmed

22118469    26354079    26227816    20798317    29403074    25069045    + More
20433749    21347285    23537049    9215903    17510324    24755649    25329095    20634964    21719571    24845553    29451363    21282665    18362917    19820115    20075255    15562597    15496914    24508170    30249741    28049606    20566863    27762356    25186727    30382153    25835551    12364791    14747013    17210077    23254933    29240929    23594743    26830274   

EMBL

AGBW02007963    OWR54365.1    NWSH01005112    PCG64430.1    ODYU01008024    SOQ51286.1    + More
KQ460367    KPJ15535.1    KQ459602    KPI93244.1    RSAL01000005    RVE54346.1    JTDY01002003    KOB72356.1    NEVH01009087    PNF33666.1    KQ414698    KOC63486.1    GL436217    EFN72229.1    PYGN01001121    PSN37421.1    PZQS01000014    PVD18929.1    KQ976482    KYM83711.1    KZ288353    PBC27303.1    KQ434773    KZC03999.1    BT079587    ACO14011.1    ADTU01003705    KB631924    ERL87224.1    AFYH01253371    AFYH01253372    AFYH01253373    AFYH01253374    AFYH01253375    CH477600    EAT38456.1    LBMM01001119    KMQ96889.1    APGK01055062    KB741259    ENN71698.1    FR904610    CDQ68087.1    JARO02002011    KPP73828.1    GU589481    ADO29431.1    GL888223    EGI64434.1    KK852617    KDR20162.1    KQ983089    KYQ47637.1    KQ980882    KYN11923.1    GDIQ01054879    JAN39858.1    RJVU01075616    ROI16089.1    GL449769    EFN82031.1    GDIQ01186524    JAK65201.1    GEBQ01018221    GEBQ01006588    GEBQ01004079    JAT21756.1    JAT33389.1    JAT35898.1    GDIP01017833    JAM85882.1    KQ435762    KOX75475.1    GL763459    EFZ19673.1    KK854583    PTY17092.1    DS231932    EDS27486.1    KQ971311    EEZ98826.2    GANO01002414    JAB57457.1    AAGJ04111440    JXJN01029586    CCAG010008016    GECU01032195    GECU01000519    JAS75511.1    JAT07188.1    CM012449    RVE65309.1    KQ427350    KOF67161.1    HAEH01004945    HAEI01006424    SBR75243.1    CAAE01014677    CAG02151.1    AXCM01000566    KK111586    QOIP01000007    EZA46339.1    RLU20153.1    HAED01018521    HAEE01005590    SBR04966.1    HADZ01010730    HAEA01008621    SBP74671.1    HADY01008617    HAEJ01012626    SBP47102.1    AJWK01017043    GEFH01002530    JAP66051.1    DS235745    EEB16215.1    HAEB01003734    HAEC01008739    SBQ76877.1    CM004469    OCT93420.1    AERX01047718    RCHS01000788    RMX56875.1    KQ042224    KKF17481.1    AAAB01008846    EAA06255.5    APCN01001139    BC114309    AMQN01004941    KB294746    ELU14124.1    GFDL01015187    JAV19858.1    HAEF01002765    HAEG01004064    SBR40147.1    GGFL01003929    MBW68107.1    GFDF01001847    JAV12237.1    CT027729    GEDV01006491    JAP82066.1   

Proteomes

UP000007151    UP000218220    UP000053240    UP000053268    UP000283053    UP000037510    + More
UP000235965    UP000053825    UP000000311    UP000245037    UP000265140    UP000245119    UP000005203    UP000078540    UP000242457    UP000076502    UP000005205    UP000030742    UP000008672    UP000192224    UP000008820    UP000036403    UP000019118    UP000193380    UP000018467    UP000034805    UP000221080    UP000007755    UP000027135    UP000261560    UP000075809    UP000261440    UP000078492    UP000008237    UP000078200    UP000053105    UP000002320    UP000087266    UP000007266    UP000002358    UP000007110    UP000092460    UP000092443    UP000092444    UP000076420    UP000257200    UP000053454    UP000007303    UP000257160    UP000265080    UP000075883    UP000092445    UP000053097    UP000279307    UP000092461    UP000261400    UP000009046    UP000186698    UP000261580    UP000005207    UP000275408    UP000007062    UP000076407    UP000075840    UP000000437    UP000014760    UP000261540    UP000265040    UP000079169    UP000075901    UP000265000    UP000242638    UP000261360   

Pfam

PF03099   BPL_LplA_LipB

Interpro

IPR020605   Octanoyltransferase_CS        + More
IPR000544   Octanoyltransferase       
IPR004143   BPL_LPL_catalytic       
IPR012337   RNaseH-like_sf       

SUPFAM

SSF53098   SSF53098       

CDD

cd16444  LipB

ProteinModelPortal

A0A212FKU4   A0A2A4IZ19   A0A2H1WEA6   A0A194RE29   A0A194PKI5   A0A437BVD1   + More
A0A0L7LAW1   A0A2J7QYL2   A0A0L7QY58   E2A2V5   A0A2P8XZI4   A0A3P8YKA1   A0A2T7NCM6   A0A088ALW7   A0A151I3F1   A0A2A3E6B0   A0A154NWE4   C1BYA8   A0A158NXY6   U4U015   H3A1P6   A0A1W4YTY4   Q0IEK7   A0A0J7L363   N6T2D6   A0A060WM11   A0A3B1KFN2   A0A0P7UV33   E3TGJ5   F4WMW4   A0A067RKW3   A0A3B3CEV7   A0A3B1JQ22   A0A1S4FN42   A0A151WIG7   A0A3B4DS71   A0A151IVW7   A0A2D0SSE5   A0A0P6FSE3   A0A3N0XI49   E2BQH3   A0A0P5KBG0   A0A1B6LDI8   A0A0P6BGB1   A0A1A9UZU6   A0A0M9A1L5   E9IID1   A0A2R7WBM1   B0WH35   A0A1S3NPT8   D6WCX6   K7IYB8   U5EXR5   W4XML7   A0A1B0AL12   A0A1A9XN22   A0A1B0FR83   A0A2C9JF27   A0A1B6HLI8   A0A3Q1ETW8   A0A437CTR0   A0A0L8FR25   A0A1A8P1U8   Q4SB31   A0A3Q1D0S5   A0A3P8TSF6   A0A182LXS1   A0A1A9ZZQ8   A0A026VU07   A0A1A8J639   A0A1A8C774   A0A1A7ZW42   A0A1B0CL88   A0A131XKJ9   A0A3B5B6N9   E0VS59   A0A1A8H0Q2   A0A1L8HBD3   A0A3Q4N1Z4   I3KKT0   A0A3M6UTA1   A0A0F8BU33   Q7PS31   A0A182XEE9   A0A182IAB4   Q29R99   R7V6C9   A0A1Q3EXD3   A0A1A8L678   A0A2M4CS08   A0A3B3QZL2   A0A3Q1J525   A0A1S3DW12   A0A1L8E0L8   F1QAW5   A0A131YV83   A0A182S5L7   A0A3Q2R0K8   A0A3P9MXY6   A0A3B4ZA36  

PDB

1W66     E-value=2.53076e-34,     Score=361

KEGG

101740788    K03801 lipoyl(octanoyl) transferase [EC:2.3.1.181] | (RefSeq) putative lipoyltransferase 2, mitochon

PATHWAY

00785    Lipoic acid metabolism - Bombyx mori (domestic silkworm)
01100    Metabolic pathways - Bombyx mori (domestic silkworm)

GO

GO:0005739   GO:0102555   GO:0033819   GO:0009249   GO:0016740   GO:0016874   GO:0016021   GO:2000376   GO:0005737   GO:0009107   GO:0016415   GO:0006464   GO:0016020   GO:0016817   GO:0008270   GO:0043565   GO:0016876   GO:0043039  

Subcellular location

Mitochondrion  

Length:

243

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.069

Exp number, first 60 AAs:

0.01177

Total prob of N-in:

0.03936

outside

1  -  243

 

 

Pi

11.919336

Theta

18.048248

Tajima's D

-0.86651

CLR

41.541363

CSRT

0.16079196040198

Interpretation

Uncertain