Gene

KWMTBOMO08802

Annotation

Nep1_protein_[Bombyx_mori]

Location

Bomo_Chr15(+):5527998-5529475      View in EpiBrowser

Full name

Ribosomal RNA small subunit methyltransferase NEP1      

Alternative Name

18S rRNA (pseudouridine-N1)-methyltransferase
Ribosome biogenesis protein NEP1

Location in the cell

Nuclear   Reliability : 2.889

CDS

ATGGGTAAAAAAAGAAAATTGGTTTCTAAAGATGATGATTTTGAATTTGATCCGATTCCTAAACATTTGGTTACTTCACACATAAAAAAGCAGGAAAAACGTCTTATAGTTATCCTGGAAAACGCACAACTGGAAAGCGTGAAAAATGGAAATAGCTTTGAGCTATTAAACTGTGATGACCATGGTCACATTTTAAGAAAAAACGATCGTGATCCTGGTTCTTGTCGCCCAGATATTACGCATCAGTCTCTCCTCATGTTGATGGACTCCCCGTTGAACAGAGCTGGCTTACTGCAGGTCTATATTCATACAGAAAAAAATGTTTTAATCGAAATTAATCCACAAACAAGAATACCTAGAACATTCAAGCGTTTTGCTGGATTAATGGTTCAACTTCTACACAAGTTTGCAATTAGAGCATCAGATGGGCCAATGAAACTTTTGAAAGTTATTAAAAATCCTGTCACGTCACATCTACCAGTTGGAGTCAAGAAAATTACTATGTCTTTTAGTTCTAAAATAGTTCAGAATTGCAGAGATCTGGTCCCAAAAGACGAACCCATTGTGATGGTCATAGGTGCAATGGCTCATGGTAAAGTTGAAGTAGACTACTCTGAAGATGTCATATCTATCAGCAATTATCCTTTATCAGCAGCATTGACTTGTGCTAAACTTTGTTCAGCATTTGAAGAAGTTTGGGGAGTAGCATAA

Protein

MGKKRKLVSKDDDFEFDPIPKHLVTSHIKKQEKRLIVILENAQLESVKNGNSFELLNCDDHGHILRKNDRDPGSCRPDITHQSLLMLMDSPLNRAGLLQVYIHTEKNVLIEINPQTRIPRTFKRFAGLMVQLLHKFAIRASDGPMKLLKVIKNPVTSHLPVGVKKITMSFSSKIVQNCRDLVPKDEPIVMVIGAMAHGKVEVDYSEDVISISNYPLSAALTCAKLCSAFEEVWGVA

Description

S-adenosyl-L-methionine-dependent pseudouridine N(1)-methyltransferase that methylates a pseudouridine in 18S rRNA. Involved the biosynthesis of the hypermodified N1-methyl-N3-(3-amino-3-carboxypropyl) pseudouridine (m1acp3-Psi) conserved in eukaryotic 18S rRNA. Has also an essential role in 40S ribosomal subunit biogenesis independent on its methyltransferase activity, facilitating the incorporation of ribosomal protein S19 during the formation of pre-ribosomes.

Catalytic Activity

a psi-uridine in 16S/18S rRNA + S-adenosyl-L-methionine = an N(1)-methylpseudouridine in 16S/18S rRNA + H(+) + S-adenosyl-L-homocysteine

Subunit

Homodimer.

Similarity

Belongs to the peptidase S1 family.
Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase NEP1 family.

Keywords

Complete proteome   Methyltransferase   Nucleus   Reference proteome   Ribosome biogenesis   RNA-binding   rRNA processing   rRNA-binding   S-adenosyl-L-methionine   Transferase  

Feature

chain  Ribosomal RNA small subunit methyltransferase NEP1

Uniprot

G9JLN1   A0A437AYJ4   A0A2A4K350   A0A1E1WAE8   S4PMR2   A0A2H1W0H8   + More
A0A194PQC2   A0A194QMD7   I4DS91   A0A0L7LGV4   A0A212F0X4   A0A0L7QPD3   A0A154PJH6   A0A1W4X6W9   A0A0M8ZQL5   E2BSS1   A0A1Y1LLY3   A0A0C9RZT2   A0A088ACM5   A0A232EM43   E9IB04   A0A151X893   F4WGN3   A0A0J7KMK6   A0A195FYR1   A0A067REU3   A0A158NP02   A0A2A3ENF2   A0A2P8Y8S5   A0A1S3D3H9   J3JWD0   A0A3L8DH33   E2AA49   A0A1B6CP32   A0A0P4VU73   R4G8U1   A0A2J7PKH9   A0A0T6B0U2   A0A023F8U3   A0A069DR15   D6WR61   E0W3P6   A0A1B6F7Y0   A0A0L0C027   A0A1B6JLB6   A0A2R7WXG5   A0A1I8ML51   A0A0A9W521   R4WJN6   A0A1I8PSV8   U5EY47   Q17J58   A0A182N783   A0A0A9VUM7   A0A146LUT0   B0WB67   A0A1J1J158   A0A182R4N3   A0A034WEK8   A0A182IRT7   A0A182W0Q3   A0A1Q3EZJ1   B3MQW5   A0A182GKQ3   A0A0K8U685   A0A0M5J121   A0A182MBG9   A0A0A1WQD6   B4M7W6   A0A2M4AV09   A0A1A9WVP2   A0A182U3C5   Q7PR76   A0A182HTM3   B4JXE2   W5JES9   A0A2M4AUY8   A0A2M4BYG0   A0A2M3ZI37   Q29JM3   B4H387   A0A1A9YME7   A0A1B0BDI6   A0A023ELI7   A0A182JSC5   A0A3B0K324   B3NU40   A0A1W4V1T3   B4Q047   B4NPH3   G7H852   Q9W4J5   W8BW52   A0A182P281   B4I0Y2   B4R4I9   A0A1D2N9W4   A0A1A9UW62   A0A0K8TM91   A0A1B0G8Q2  

EC Number

2.1.1.-

Pubmed

23622113    26354079    22651552    26227816    22118469    20798317    + More
28004739    28648823    21282665    21719571    24845553    21347285    29403074    22516182    23537049    30249741    27129103    25474469    26334808    18362917    19820115    20566863    26108605    25315136    25401762    26823975    23691247    17510324    25348373    17994087    26483478    25830018    12364791    14747013    17210077    20920257    23761445    15632085    24945155    17550304    10731132    12537572    24495485    27289101    26369729   

EMBL

JN872356    AEV53624.1    RSAL01000266    RVE43256.1    NWSH01000179    PCG78691.1    + More
GDQN01007213    JAT83841.1    GAIX01000041    JAA92519.1    ODYU01005599    SOQ46599.1    KQ459596    KPI95512.1    KQ461195    KPJ06703.1    AK405476    BAM20781.1    JTDY01001240    KOB74436.1    AGBW02011012    OWR47380.1    KQ414819    KOC60495.1    KQ434936    KZC11977.1    KQ435899    KOX69115.1    GL450241    EFN81317.1    GEZM01051890    JAV74649.1    GBYB01013646    JAG83413.1    NNAY01003448    OXU19401.1    GL762091    EFZ22240.1    KQ982422    KYQ56596.1    GL888140    EGI66643.1    LBMM01005476    KMQ91481.1    KQ981161    KYN45437.1    KK852732    KDR17463.1    ADTU01021954    KZ288204    PBC33247.1    PYGN01000801    PSN40659.1    APGK01043560    BT127548    KB741015    KB632161    AEE62510.1    ENN75316.1    ERL89246.1    QOIP01000008    RLU19626.1    GL438029    EFN69687.1    GEDC01022174    JAS15124.1    GDKW01000495    JAI56100.1    ACPB03016588    GAHY01000382    JAA77128.1    NEVH01024540    PNF16843.1    LJIG01016309    KRT80988.1    GBBI01000882    JAC17830.1    GBGD01002738    JAC86151.1    KQ971354    EFA07025.1    DS235882    EEB20252.1    GECZ01023437    JAS46332.1    JRES01001169    KNC24819.1    GECU01007741    JAS99965.1    KK855878    PTY24229.1    GBHO01043644    GBHO01043643    GDHC01010063    JAF99959.1    JAF99960.1    JAQ08566.1    AK417785    BAN21000.1    GANO01002200    JAB57671.1    CH477234    EAT46749.1    GBHO01043642    JAF99961.1    GDHC01008499    JAQ10130.1    DS231877    EDS42132.1    CVRI01000064    CRL05174.1    GAKP01006367    JAC52585.1    GFDL01014320    JAV20725.1    CH902622    EDV34170.2    JXUM01070405    KQ562604    KXJ75505.1    GDHF01030459    JAI21855.1    CP012528    ALC48986.1    AXCM01000194    GBXI01013649    JAD00643.1    CH940653    EDW62883.2    GGFK01011293    MBW44614.1    AAAB01008859    EAA07709.3    APCN01000570    CH916376    EDV95418.1    ADMH02001605    ETN61848.1    GGFK01011274    MBW44595.1    GGFJ01008911    MBW58052.1    GGFM01007347    MBW28098.1    CH379063    EAL32278.1    CH479205    EDW30780.1    JXJN01012502    GAPW01003506    JAC10092.1    OUUW01000003    SPP77798.1    CH954180    EDV45816.1    CM000162    EDX01199.1    CH964291    EDW86413.1    BT132776    AET62581.1    AE014298    GAMC01001035    GAMC01001034    JAC05521.1    CH480819    EDW53163.1    CM000366    EDX17079.1    LJIJ01000126    ODN02049.1    GDAI01002121    JAI15482.1    CCAG010000299   

Proteomes

UP000283053    UP000218220    UP000053268    UP000053240    UP000037510    UP000007151    + More
UP000053825    UP000076502    UP000192223    UP000053105    UP000008237    UP000005203    UP000215335    UP000075809    UP000007755    UP000036403    UP000078541    UP000027135    UP000005205    UP000242457    UP000245037    UP000079169    UP000019118    UP000030742    UP000279307    UP000000311    UP000015103    UP000235965    UP000007266    UP000009046    UP000037069    UP000095301    UP000095300    UP000008820    UP000075884    UP000002320    UP000183832    UP000075900    UP000075880    UP000075920    UP000007801    UP000069940    UP000249989    UP000092553    UP000075883    UP000008792    UP000091820    UP000075902    UP000007062    UP000075840    UP000001070    UP000000673    UP000001819    UP000008744    UP000092443    UP000092460    UP000075881    UP000268350    UP000008711    UP000192221    UP000002282    UP000007798    UP000000803    UP000075885    UP000001292    UP000000304    UP000094527    UP000078200    UP000092444   

PRIDE

G9JLN1    Q9W4J5   

Pfam

PF03587   EMG1        + More
PF00089   Trypsin

Interpro

IPR029026   tRNA_m1G_MTases_N        + More
IPR029028   Alpha/beta_knot_MTases       
IPR005304   Rbsml_bgen_MeTrfase_EMG1/NEP1       
IPR009003   Peptidase_S1_PA       
IPR001314   Peptidase_S1A       
IPR018114   TRYPSIN_HIS       
IPR001254   Trypsin_dom       
IPR033116   TRYPSIN_SER       

SUPFAM

SSF75217   SSF75217        + More
SSF50494   SSF50494       

Gene 3D

3.40.1280.10    

CDD

cd00190  Tryp_SPc

ProteinModelPortal

G9JLN1   A0A437AYJ4   A0A2A4K350   A0A1E1WAE8   S4PMR2   A0A2H1W0H8   + More
A0A194PQC2   A0A194QMD7   I4DS91   A0A0L7LGV4   A0A212F0X4   A0A0L7QPD3   A0A154PJH6   A0A1W4X6W9   A0A0M8ZQL5   E2BSS1   A0A1Y1LLY3   A0A0C9RZT2   A0A088ACM5   A0A232EM43   E9IB04   A0A151X893   F4WGN3   A0A0J7KMK6   A0A195FYR1   A0A067REU3   A0A158NP02   A0A2A3ENF2   A0A2P8Y8S5   A0A1S3D3H9   J3JWD0   A0A3L8DH33   E2AA49   A0A1B6CP32   A0A0P4VU73   R4G8U1   A0A2J7PKH9   A0A0T6B0U2   A0A023F8U3   A0A069DR15   D6WR61   E0W3P6   A0A1B6F7Y0   A0A0L0C027   A0A1B6JLB6   A0A2R7WXG5   A0A1I8ML51   A0A0A9W521   R4WJN6   A0A1I8PSV8   U5EY47   Q17J58   A0A182N783   A0A0A9VUM7   A0A146LUT0   B0WB67   A0A1J1J158   A0A182R4N3   A0A034WEK8   A0A182IRT7   A0A182W0Q3   A0A1Q3EZJ1   B3MQW5   A0A182GKQ3   A0A0K8U685   A0A0M5J121   A0A182MBG9   A0A0A1WQD6   B4M7W6   A0A2M4AV09   A0A1A9WVP2   A0A182U3C5   Q7PR76   A0A182HTM3   B4JXE2   W5JES9   A0A2M4AUY8   A0A2M4BYG0   A0A2M3ZI37   Q29JM3   B4H387   A0A1A9YME7   A0A1B0BDI6   A0A023ELI7   A0A182JSC5   A0A3B0K324   B3NU40   A0A1W4V1T3   B4Q047   B4NPH3   G7H852   Q9W4J5   W8BW52   A0A182P281   B4I0Y2   B4R4I9   A0A1D2N9W4   A0A1A9UW62   A0A0K8TM91   A0A1B0G8Q2  

PDB

5FAI     E-value=2.30794e-86,     Score=810

KEGG

100862801    K14568 rRNA small subunit pseudouridine methyltransferase Nep1 [EC:2.1.1.260] | (RefSeq) Nep1; Nep1

PATHWAY

03008    Ribosome biogenesis in eukaryotes - Bombyx mori (domestic silkworm)

GO

GO:0008168   GO:0005730   GO:0070037   GO:0019843   GO:0032040   GO:0070475   GO:0004252   GO:0042742   GO:0031167   GO:0006914   GO:0005840   GO:0006412   GO:0008097   GO:0016817   GO:0008270   GO:0043565   GO:0016876   GO:0043039  

PANTHER

PTHR12636  

Subcellular location

  

Length:

236

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.01981

Exp number, first 60 AAs:

0

Total prob of N-in:

0.20510

outside

1  -  236

 

 

Pi

294.488839

Theta

226.953848

Tajima's D

0.937094

CLR

0

CSRT

0.64266786660667

Interpretation

Possibly Positive selection