Gene

KWMTBOMO08652  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA007637

Annotation

glycine--tRNA_ligase_[Bombyx_mori]

Location

Bomo_Chr15(+):1644293-1648806      View in EpiBrowser

Full name

Glycine--tRNA ligase      

Alternative Name

Diadenosine tetraphosphate synthetase
Glycyl-tRNA synthetase

Location in the cell

Cytoplasmic   Reliability : 3.411

CDS

ATGGCCGATCCTAAGATTGAAGAAATCCTCGCTCCTTTGAGAGCTAATGTGAAAGAACAAGGCGATTTGGTTAGAAAACTTAAAGAAGAAAAAGCACCCGAAATTGATATAAAGAAGGCAGTCGCTGAATTAAAAACTAGGAAGAAGATATTAGAAGACAAAGAGCTGAGCCTCGCACCCGCGGAAGATTTGTTTGACCGTGCTAAAATGGAAGATCTAATTAAAAGGCGCTTTTTCTATGACCAGTCTTTTGCTATATATGGTGGAATTACTGGCCAGTTTGATTTTGGTCCTATGGGATGTGCACTTAAGAGTAACATGATCCACTTATGGAAGAAGTTTTTTATTCTTCAGGAACAAATGTTAGAGGTAGAATGCTCAATTCTTACTCCAGAACCTGTACTCAAAGCCTCAGGTCATGTGGAGAGATTTGCAGACTTGATGACCAAGGATATTAAAACAGGTGAGTGCTTCCGGCTTGACCATTTAATTAAAGGACACCTTGAAAAAATCAAATCTGATAAGAACACCAAAATTGAATTGAAGGCAGAGATTGAAGATATTCTTATAAAACTAGATGGAATGAATGCTGACGAGATGTCAGCCTTGATGAAAAGATTTGAAATGAAATCACCTATTAGTGGAAATGATTTAACTCCTCCAATAGAATTTAACCTAATGTTTAACACACAAATAGGCCCATCAGGCTTAGTAAAAGGTTTTCTAAGGCCCGAAACAGCTCAAGGCATTTTTGTTAACTTCAAACGTCTTTTAGAGTTCAATCAAGGACGTTTACCATTTGCAGCTGCACAAATAGGAAATTCCTTCAGAAATGAAATTTCACCTCGATCAGGTCTCCTCAGAGTTAGAGAATTTACTATGTGTGAAATTGAGCATTTTTGTGATGTTAAAGAGCATCCAAAGTTTGAATCTGTAAAAAACACTCAGATGCTATTGTATTCTGCTGATAATCAGGAACAAGGTAAACCTGCAGAGCTAACAACCATTGGAGATGCTGTATCGAAAGGCATTGTTAATAATGAAACACTTGGGTATTTCATGGCAAGAATTCACATGTATATGTTGGCTGTTGGTATTGATCCTAAGAGACTGAGATTCAGACAGCACATGGGCAATGAAATGGCTCACTATGCATGTGACTGTTGGGATGCAGAGTGTCTGAGTAGCTATGGCTGGATCGAGTGTGTTGGATGTGCTGATAGATCAGCTTATGATTTAACACAACACACAAAAGCTACTGGAATCAGATTGGCTGCAGAAAAGAAACTTCCAGCTCCAAAACAGATAGAAGTTGTTGAAGCCATTGCCAACAAGGCAGCCATTGGAAAGGCATTTAAAAAAGATTCCCAAGCCATTAATGATACTTTGGCTACATTAGACAACGCTGCTTTAGAAGAGATGCAAAAAGAACTTGACAGTAATGGTGAATACACGCTTATTACTGCTAGAGGAGAATTCAAACTAACTCCCAGCCTTGTGAATGTTAAGAAGACTCAAAAAACAATTCATGTAGAGGAAATTATACCAAGTGTCATTGAACCTTCATTTGGTGTTGGTAGAATTTTATACTGCATTTTAGAACATAACTTTAGGATGAGGGAGGGAGATGAACAAAGAACATATTTTTCACTTCCACCAACAGTTGCTCCCATGAAATGTGTTGTTCTACCCTTGAGTGGTAATGCAGAATTCCAGCCATTCGTAAGAGATCTTTCTCAAGAGCTGATCACAGTTGATGTTTCTCATAAGGTTGATGACTCCTCAGGTTCAATCGGCCGTCGTTATGCCAGAACTGATGAACTGGGAGTGCCTTATGCCGTGACTGTCGATTTTGATACAATTAAAGAACCCCACACAGTCACCCTTAGAGAAAGGGATAGTATGAGGCAAGTAAGATTGCCCATGGCAGATGTTCCCACAGTTGTAAGGGATTTATCTAATAGCAAAATATTGTGGTCTGATGTTGAGCAGAAATATCCTAAGTTTGAACAACAAGAAACTGTGAAAGGTACATCTGTGTAA

Protein

MADPKIEEILAPLRANVKEQGDLVRKLKEEKAPEIDIKKAVAELKTRKKILEDKELSLAPAEDLFDRAKMEDLIKRRFFYDQSFAIYGGITGQFDFGPMGCALKSNMIHLWKKFFILQEQMLEVECSILTPEPVLKASGHVERFADLMTKDIKTGECFRLDHLIKGHLEKIKSDKNTKIELKAEIEDILIKLDGMNADEMSALMKRFEMKSPISGNDLTPPIEFNLMFNTQIGPSGLVKGFLRPETAQGIFVNFKRLLEFNQGRLPFAAAQIGNSFRNEISPRSGLLRVREFTMCEIEHFCDVKEHPKFESVKNTQMLLYSADNQEQGKPAELTTIGDAVSKGIVNNETLGYFMARIHMYMLAVGIDPKRLRFRQHMGNEMAHYACDCWDAECLSSYGWIECVGCADRSAYDLTQHTKATGIRLAAEKKLPAPKQIEVVEAIANKAAIGKAFKKDSQAINDTLATLDNAALEEMQKELDSNGEYTLITARGEFKLTPSLVNVKKTQKTIHVEEIIPSVIEPSFGVGRILYCILEHNFRMREGDEQRTYFSLPPTVAPMKCVVLPLSGNAEFQPFVRDLSQELITVDVSHKVDDSSGSIGRRYARTDELGVPYAVTVDFDTIKEPHTVTLRERDSMRQVRLPMADVPTVVRDLSNSKILWSDVEQKYPKFEQQETVKGTSV

Description

Catalyzes the ATP-dependent ligation of glycine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (Gly-AMP). Also produces diadenosine tetraphosphate (Ap4A), a universal pleiotropic signaling molecule needed for cell regulation pathways, by direct condensation of 2 ATPs. Thereby, may play a special role in Ap4A homeostasis.

Catalytic Activity

ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly)
2 ATP + H(+) = diphosphate + P(1),P(4)-bis(5'-adenosyl) tetraphosphate

Subunit

Homodimer.

Similarity

Belongs to the class-II aminoacyl-tRNA synthetase family.

Keywords

Aminoacyl-tRNA synthetase   ATP-binding   Complete proteome   Direct protein sequencing   Ligase   Nucleotide-binding   Protein biosynthesis   Reference proteome   Transferase  

Feature

chain  Glycine--tRNA ligase

Uniprot

H9JDP0   Q2F5I5   Q04451   S4PY59   A0A2A4K7H6   A0A437BXL4   + More
A0A2H1VBH5   A0A0L7LJW2   A0A194PQZ8   A0A0N1IQ54   A0A182YQI0   A0A182NTC9   A0A182MJ81   A0A2M4BG41   A0A182QJ69   W5JSI5   A0A182F0T9   A0A182TM26   A0A182V536   A0A1S4GYQ9   A0A1S4GYQ3   A0A182KMF4   A0A2M4BGD6   A0A182X5Z1   Q5TS92   A0A2M4A8J1   A0A182RK81   A0A182WFJ5   A0A182IBM3   A0A182P4B4   A0A2M4A9I3   A0A182J6G3   U5EUQ2   Q178X7   A0A182GAV1   A0A1Q3F5H4   A0A067R1U2   A0A1S4JU77   B0WUQ5   A0A139WHY9   A0A195CUA6   D6WJF7   A0A1J1I3K6   A0A151IZ28   A0A195ATP8   A0A1W4WY78   A0A0P5QKL0   A0A1Y1LFS2   A0A195F7D7   A0A0P6HD92   F4WZA1   A0A0P6H414   K7IMD6   A0A0P6G1L8   A0A0P5KSH0   E2B5E8   A0A164Y5F7   A0A154PEH1   A0A0P6GGL2   A0A0P5P350   A0A0P4YFB4   A0A0P5LGG4   A0A0P4X8V8   A0A0P5MWG2   A0A0J7KVP2   A0A026X1N7   A0A0N8E0Q3   A0A0C9Q756   E9FYJ6   E9IRQ3   A0A0T6B2M6   A0A232EXD8   A0A0P6CRY0   A0A0K8TRC6   U4UMY2   A0A2A3EMW2   A0A151X7C3   A0A0L0CE99   A0A0N0U4S8   A0A088AKB2   T1PDJ9   A0A1I8M461   A0A1L8DFH3   A0A411G6X4   A0A3Q0IVX0   W8BJ39   A0A034WFT8   A0A1E1X3R7   A0A0A1WL86   A0A034WE93   A0A0K8U012   W8BPQ4   A0A131XIK8   A0A0K8WL73   A0A0P5HRX3   A0A0P5PQF6   A0A1I8PI77   A0A023FNV6   A0A2R5L7I3   A0A293MVW1  

EC Number

6.1.1.14

Pubmed

19121390    8463296    23622113    26227816    26354079    25244985    + More
20920257    23761445    12364791    20966253    17510324    26483478    24845553    18362917    19820115    28004739    21719571    20075255    20798317    24508170    30249741    21292972    21282665    28648823    26369729    23537049    26108605    25315136    24495485    25348373    28503490    25830018    28049606   

EMBL

BABH01003232    DQ311438    ABD36382.1    L08106    GAIX01003858    JAA88702.1    + More
NWSH01000087    PCG79723.1    RSAL01000001    RVE55206.1    ODYU01001486    SOQ37752.1    JTDY01000839    KOB75705.1    KQ459596    KPI95383.1    KQ459781    KPJ19986.1    AXCM01005597    GGFJ01002879    MBW52020.1    AXCN02000715    ADMH02000293    ETN67091.1    AAAB01008944    GGFJ01002880    MBW52021.1    EAL40245.2    GGFK01003731    MBW37052.1    APCN01000710    GGFK01003957    MBW37278.1    GANO01001367    JAB58504.1    CH477357    EAT42765.2    JXUM01051580    KQ561695    KXJ77781.1    GFDL01012235    JAV22810.1    KK853090    KDR11569.1    DS232108    EDS35041.1    KQ971342    KYB27572.1    KQ977305    KYN03724.1    EFA03680.2    CVRI01000037    CRK93446.1    KQ980735    KYN13767.1    KQ976741    KYM75571.1    GDIQ01119170    JAL32556.1    GEZM01057003    JAV72512.1    KQ981756    KYN36097.1    GDIQ01043605    JAN51132.1    GL888470    EGI60490.1    GDIQ01241093    GDIQ01035021    JAN59716.1    GDIQ01041162    JAN53575.1    GDIQ01242542    GDIQ01187300    JAK64425.1    GL445816    EFN89098.1    LRGB01000930    KZS14907.1    KQ434889    KZC10262.1    GDIQ01033426    JAN61311.1    GDIQ01135823    JAL15903.1    GDIP01229269    JAI94132.1    GDIQ01195152    JAK56573.1    GDIP01244756    JAI78645.1    GDIQ01152518    JAK99207.1    LBMM01002814    KMQ94329.1    KK107024    QOIP01000004    EZA62215.1    RLU23269.1    GDIQ01073244    JAN21493.1    GBYB01009978    JAG79745.1    GL732527    EFX87538.1    GL765283    EFZ16704.1    LJIG01016180    KRT81374.1    NNAY01001740    OXU23066.1    GDIQ01088230    JAN06507.1    GDAI01000920    JAI16683.1    KB632288    ERL91526.1    KZ288217    PBC32381.1    KQ982450    KYQ56210.1    JRES01000502    KNC30571.1    KQ435808    KOX72994.1    KA646836    AFP61465.1    GFDF01008893    JAV05191.1    MH365873    QBB01682.1    GAMC01007798    JAB98757.1    GAKP01005942    JAC53010.1    GFAC01005304    JAT93884.1    GBXI01014665    JAC99626.1    GAKP01005943    JAC53009.1    GDHF01032235    GDHF01022663    GDHF01008967    JAI20079.1    JAI29651.1    JAI43347.1    GAMC01007797    JAB98758.1    GEFH01001622    JAP66959.1    GDHF01001100    GDHF01000418    JAI51214.1    JAI51896.1    GDIQ01229141    JAK22584.1    GDIQ01125286    JAL26440.1    GBBK01002124    JAC22358.1    GGLE01001306    MBY05432.1    GFWV01019340    MAA44068.1   

Proteomes

UP000005204    UP000218220    UP000283053    UP000037510    UP000053268    UP000053240    + More
UP000076408    UP000075884    UP000075883    UP000075886    UP000000673    UP000069272    UP000075902    UP000075903    UP000075882    UP000076407    UP000007062    UP000075900    UP000075920    UP000075840    UP000075885    UP000075880    UP000008820    UP000069940    UP000249989    UP000027135    UP000002320    UP000007266    UP000078542    UP000183832    UP000078492    UP000078540    UP000192223    UP000078541    UP000007755    UP000002358    UP000008237    UP000076858    UP000076502    UP000036403    UP000053097    UP000279307    UP000000305    UP000215335    UP000030742    UP000242457    UP000075809    UP000037069    UP000053105    UP000005203    UP000095301    UP000079169    UP000095300   

PRIDE

H9JDP0    Q2F5I5    Q04451    A0A088AKB2   

Pfam

PF00587   tRNA-synt_2b        + More
PF00458   WHEP-TRS
PF03129   HGTP_anticodon
PF04420   CHD5
PF00098   zf-CCHC
PF00076   RRM_1

Interpro

IPR006195   aa-tRNA-synth_II        + More
IPR004154   Anticodon-bd       
IPR027031   Gly-tRNA_synthase/POLG2       
IPR036621   Anticodon-bd_dom_sf       
IPR002315   tRNA-synt_gly       
IPR033731   GlyRS-like_core       
IPR002314   aa-tRNA-synt_IIb       
IPR000738   WHEP-TRS_dom       
IPR009068   S15_NS1_RNA-bd       
IPR028945   WRB/Get1       
IPR000504   RRM_dom       
IPR012677   Nucleotide-bd_a/b_plait_sf       
IPR001878   Znf_CCHC       
IPR035979   RBD_domain_sf       

SUPFAM

SSF47060   SSF47060        + More
SSF54928   SSF54928       

Gene 3D

3.40.50.800     3.30.70.330    

CDD

cd00774  GlyRS-like_core

ProteinModelPortal

H9JDP0   Q2F5I5   Q04451   S4PY59   A0A2A4K7H6   A0A437BXL4   + More
A0A2H1VBH5   A0A0L7LJW2   A0A194PQZ8   A0A0N1IQ54   A0A182YQI0   A0A182NTC9   A0A182MJ81   A0A2M4BG41   A0A182QJ69   W5JSI5   A0A182F0T9   A0A182TM26   A0A182V536   A0A1S4GYQ9   A0A1S4GYQ3   A0A182KMF4   A0A2M4BGD6   A0A182X5Z1   Q5TS92   A0A2M4A8J1   A0A182RK81   A0A182WFJ5   A0A182IBM3   A0A182P4B4   A0A2M4A9I3   A0A182J6G3   U5EUQ2   Q178X7   A0A182GAV1   A0A1Q3F5H4   A0A067R1U2   A0A1S4JU77   B0WUQ5   A0A139WHY9   A0A195CUA6   D6WJF7   A0A1J1I3K6   A0A151IZ28   A0A195ATP8   A0A1W4WY78   A0A0P5QKL0   A0A1Y1LFS2   A0A195F7D7   A0A0P6HD92   F4WZA1   A0A0P6H414   K7IMD6   A0A0P6G1L8   A0A0P5KSH0   E2B5E8   A0A164Y5F7   A0A154PEH1   A0A0P6GGL2   A0A0P5P350   A0A0P4YFB4   A0A0P5LGG4   A0A0P4X8V8   A0A0P5MWG2   A0A0J7KVP2   A0A026X1N7   A0A0N8E0Q3   A0A0C9Q756   E9FYJ6   E9IRQ3   A0A0T6B2M6   A0A232EXD8   A0A0P6CRY0   A0A0K8TRC6   U4UMY2   A0A2A3EMW2   A0A151X7C3   A0A0L0CE99   A0A0N0U4S8   A0A088AKB2   T1PDJ9   A0A1I8M461   A0A1L8DFH3   A0A411G6X4   A0A3Q0IVX0   W8BJ39   A0A034WFT8   A0A1E1X3R7   A0A0A1WL86   A0A034WE93   A0A0K8U012   W8BPQ4   A0A131XIK8   A0A0K8WL73   A0A0P5HRX3   A0A0P5PQF6   A0A1I8PI77   A0A023FNV6   A0A2R5L7I3   A0A293MVW1  

PDB

2Q5H     E-value=0,     Score=2329

KEGG

692991    K01880 glycyl-tRNA synthetase [EC:6.1.1.14] | (RefSeq) glycyl-tRNA synthetase

PATHWAY

00970    Aminoacyl-tRNA biosynthesis - Bombyx mori (domestic silkworm)

GO

GO:0005737   GO:0006426   GO:0004820   GO:0005524   GO:0046983   GO:0015966   GO:0016740   GO:0005739   GO:0070150   GO:0071816   GO:0016021   GO:0008270   GO:0003723   GO:0016874   GO:0004812   GO:0006418   GO:0000166   GO:0003676   GO:0016876   GO:0043039  

PANTHER

PTHR10745  

Length:

680

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.16847

Exp number, first 60 AAs:

0

Total prob of N-in:

0.00792

outside

1  -  680

 

 

Pi

38.712614

Theta

53.773165

Tajima's D

-1.000592

CLR

539.665569

CSRT

0.136343182840858

Interpretation

Uncertain