Gene

KWMTBOMO08504  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA009359

Annotation

DNA_polymerase_subunit_gamma-1?_mitochondrial_[Papilio_machaon]

Location

Bomo_Chr14(+):11019374-11028368      View in EpiBrowser

Full name

Glutamyl-tRNA(Gln) amidotransferase subunit C, mitochondrial       + More
DNA polymerase subunit gamma-1, mitochondrial      

Alternative Name

Mitochondrial DNA polymerase catalytic subunit

Location in the cell

Cytoplasmic   Reliability : 2.655

CDS

ATGATTTCTAAGAATATATATGAGCAACTGTTTAAAAAGCCCAGTTCTTCACCAGATCCTGAGGTTATTAAAAGCTGCCAAGAAAGTTTACTCCGACATGGCATAGATTACAAAAAGTGCAGTAATTATTTACCTGATGTAACTCTTAAATTGCCCAAATTAGAAGGTGAAGATATAGTGGAGCATTTTCATAACATTGGTGAGAAGCAGTGTGCTCCCTATAGGAGATTGATAAAAGATTTGGCTGGTGCAAGACTGCCTAATTTACCAAAGAAATGGTCTAAAACCTGTGGTTGGAGTAGATATACTGACAACAAAATCGAAGCAATACCATGCCCTCCAGATGATGCTCTTATATTTGATGTAGAGGTTCTTATGTCAGCAGGCAAGAGACCCACATTGGCATGTGCGGTTTCTGCCAATGCATGGTACAGTTGGGTCAGTGAACCTGTTGCAAATGAGGAAACTCATAAATACTTCGAGAGTGTTAACTACGAAGACCTGATACCTCTGGAGACGGACGGCATTAATCCTTGTGGAGATCTGCAAAGGCCTCGCATAGTCATAGGCCACAATGTTTCTTACGACAGAGCCAAGATTAAGGAACAATACTGGCCAAATAAAACAGGCGTACGTTTCATGGACACGATGTCCATGCACACGTGCGTGAGCGGGGTGACCAGCTACCAGAGAACTGTCCTGAAGACAAAGAACAAGGAGCCAGATCCCTCCGACGAAGACTGGATCGAGATAAGCTCCTTGAATAGTTTATCTGAGGTCCACAAGCTGTACTGCGGCAGAACCGTAGACAAAGCGACGCGAGACATCTTTGTGGACGGCACAATGCAAGACGTCCACAGTAACTTTCAGAAACTAATGAATTACTGCGCTGCTGACGTCATCGCGACACATAACGTACTAAATCGCCTACTGCCGATGTTCTTAGAAAGGTTCCCGCATCCCGTGACGTTAGCGGGGATGCTCGAATTAGGTATGGCGTACCTACCTATAAATTCGAATTGGACTCAGTACATAGATTCGGCTGAAACAGTATTCGAGGATATGAAGCTTGAGTCGCGTCAGTTATTGTCTTTGAAGGCCGATGAGGCGTGTCGTCTCATGGAGAATGAGGCCTACAAAAAGGACCTCTGGATGTGGGACCAGGACTGGTCGGTGCTGAAATTAAAATTAAAAAAACAAACAATCAAAAAAAAGAACGTTGTCGCCGAAGTAAATACGGAAGCGGATGTCGAATGCACTAATCGAAAATTGACCAATGAGAGCACAGAAGAAGTTCCAACTCAAGAAAAAACATTTGATATTCTGAACGAGGAATATATAGATTACGTTGAAAATGCTAACAGGATCAAAGGTACTGGCGCAGATCGAACGGAAGCAGAATTCAAATATTTATTCGATCTCGAGAAGTTGTTGCCAGTTAAAAGGCCTCTGTTGCCCGGATACCCGGCTTGGTATCGGAAACTGTGCACGAAGCCCGGCAAAGATCCGGATTGGGCGCCTGGAGCCAACAACATCACGACAAGTATGCAGATAACTCCAAAACTCCTGCGTCTATCCTGGGAAGGTTACCCCCTGCACCACGTAAAGTCTGAGGGCTGGGGCTTCATGGTTCCATTCAGCAGGGAGGTACGCGACGAGGAGAACCCTCGCCTGCCTCTCGAGCAGCTGTTGGAGAAATGCCCTTTGCTATTCAGTAAGGGAGAAGTGGATACTGATTTGTATGTACTACCGAAGACTGTTGAGGAAGACCTCGGCAGACGGGCGTACTACGCGAGAAAGACTATCGAAGAGAAGGCTGCTGCTAATCAGTACCACGGCCTCGGAGTGTGGTGCGGCATACAGCTGCAAGGTTGTTGCCATTTTTTGAGACTACCACACAAAGACGGGCCGAAATACAAAGTGGGGAATCCCCTGGCGAAGGATTTCTTGGACATGTTCAGCCAGAACGTGCTCTCCGCTCAGGGGAACGAAGCTGAAAAGGTACCTTTCAAATGA

Protein

MISKNIYEQLFKKPSSSPDPEVIKSCQESLLRHGIDYKKCSNYLPDVTLKLPKLEGEDIVEHFHNIGEKQCAPYRRLIKDLAGARLPNLPKKWSKTCGWSRYTDNKIEAIPCPPDDALIFDVEVLMSAGKRPTLACAVSANAWYSWVSEPVANEETHKYFESVNYEDLIPLETDGINPCGDLQRPRIVIGHNVSYDRAKIKEQYWPNKTGVRFMDTMSMHTCVSGVTSYQRTVLKTKNKEPDPSDEDWIEISSLNSLSEVHKLYCGRTVDKATRDIFVDGTMQDVHSNFQKLMNYCAADVIATHNVLNRLLPMFLERFPHPVTLAGMLELGMAYLPINSNWTQYIDSAETVFEDMKLESRQLLSLKADEACRLMENEAYKKDLWMWDQDWSVLKLKLKKQTIKKKNVVAEVNTEADVECTNRKLTNESTEEVPTQEKTFDILNEEYIDYVENANRIKGTGADRTEAEFKYLFDLEKLLPVKRPLLPGYPAWYRKLCTKPGKDPDWAPGANNITTSMQITPKLLRLSWEGYPLHHVKSEGWGFMVPFSREVRDEENPRLPLEQLLEKCPLLFSKGEVDTDLYVLPKTVEEDLGRRAYYARKTIEEKAAANQYHGLGVWCGIQLQGCCHFLRLPHKDGPKYKVGNPLAKDFLDMFSQNVLSAQGNEAEKVPFK

Description

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).
Involved in the replication of mitochondrial DNA. Has both 5'-3' DNA polymerase and 3'-5' exonuclease activity.

Catalytic Activity

ATP + H2O + L-glutamine + L-glutamyl-tRNA(Gln) = ADP + H(+) + L-glutamate + L-glutaminyl-tRNA(Gln) + phosphate
a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1)

Cofactor

Mg(2+)

Subunit

Subunit of the heterotrimeric GatCAB amidotransferase (AdT) complex, composed of A, B and C subunits.
Heterotrimer composed of a catalytic subunit and a homodimer of accessory subunits.

Similarity

Belongs to the adaptor complexes medium subunit family.
Belongs to the GatC family.
Belongs to the DNA polymerase type-A family.

Keywords

Complete proteome   Direct protein sequencing   DNA replication   DNA-binding   DNA-directed DNA polymerase   Magnesium   Mitochondrion   Nucleotidyltransferase   Reference proteome   Transferase   Transit peptide  

Feature

chain  DNA polymerase subunit gamma-1, mitochondrial

Uniprot

H9JIK9   A0A2W1BGU1   A0A2A4JVT1   A0A2H1VVJ9   A0A437B739   A0A0N1PHG4   + More
A0A212EKX6   A0A194QD71   A0A182HFD6   A0A067QMN5   A0A182US34   Q7QET4   A0A1S4G836   A0A2Z5U602   A0A2P8YGW7   A0A182XQ05   A0A1J1IL40   A0A2C9GQN8   A0A1B0EW77   A0A182TD53   A0A0J7KY38   B0WYY9   A0A182YK54   A0A158P1B2   A0A151J3N1   A0A182MJW0   A0A182W543   A0A182SA91   A0A182K7I2   D2A2U3   A0A034W6H6   E2A680   A0A182FJT7   A0A1Y1KQB2   A0A1Q3EWH2   E9IKY1   A0A182RCF6   A0A1Y1KTR5   A0A182N1E0   A0A026W4E4   F4W9B2   A0A0K8V1M3   A0A1W4UUS9   A0A2M4AC42   A0A0A1WGH5   A0A182PVA5   A0A084WFR4   B3NAD1   Q27607   A0A182ILV4   A0A3B0KFX3   B4GX06   B4P3Q5   B4N0X4   A0A1A9V9J6   Q29K74   A0A0J9TMZ7   A0A1I8MKW9   B4HXA2   B3MN26   A0A0C9R4X0   A0A336KHX2   A0A182QTQ9   N6T9G9   B4JCX4   A0A2A3ER84   W5JT93   U4UQW6   A0A1B0B7M3   B4KIY0   B4M8L4   A0A0N0BDZ2   A0A0N8NZ99   A0A1I8Q0N8   A0A1B0FDD3   A0A0L7RK58   A0A1B0BJF8   A0A1B0A530   A0A088AV73   A0A1B6D0L6   A0A2J7PCY6   E2BGS0   A0A0T6AVN1   A0A1B6JZM3   A0A0A9WXR6   A0A146LZN8   X1WIQ6   A0A2R7W845   A0A131XXV7   B7QJ35   E0VHX3   A0A0V0G5V2   A0A069DX00   T1HWN3   A0A224XIJ0   A0A131YUP9   A0A2J7PCY7   A0A2J7PCY9   A0A1E1XD11   A0A0P7WZF3  

EC Number

6.3.5.-
2.7.7.7

Pubmed

19121390    28756777    26354079    22118469    26483478    24845553    + More
12364791    26760975    29403074    25244985    21347285    18362917    19820115    25348373    20798317    28004739    21282665    24508170    30249741    21719571    25830018    24438588    17994087    8884268    8798543    10471707    10731132    12537572    12537569    17550304    15632085    22936249    25315136    23537049    20920257    23761445    25401762    26823975    20566863    26334808    26830274    28503490   

EMBL

BABH01027089    BABH01027090    BABH01027091    BABH01027092    KZ150432    PZC70893.1    + More
NWSH01000476    PCG76135.1    ODYU01004704    SOQ44865.1    RSAL01000133    RVE46330.1    KQ459928    KPJ19301.1    AGBW02014173    OWR42143.1    KQ459337    KPJ01401.1    JXUM01134030    JXUM01134031    JXUM01134032    JXUM01134033    KQ568063    KXJ69158.1    KK853147    KDR10711.1    AAAB01008846    EAA06413.5    FX985751    BBA93638.1    PYGN01000603    PSN43497.1    CVRI01000053    CRK99798.1    APCN01003967    AJWK01005375    LBMM01002131    KMQ95236.1    DS232201    EDS37252.1    ADTU01001055    KQ980275    KYN17013.1    AXCM01000617    KQ971339    EFA02782.2    GAKP01007776    JAC51176.1    GL437116    EFN71060.1    GEZM01076843    JAV63602.1    GFDL01015383    JAV19662.1    GL764026    EFZ18754.1    GEZM01076844    JAV63600.1    KK107488    QOIP01000012    EZA49914.1    RLU15840.1    GL888002    EGI69294.1    GDHF01019621    JAI32693.1    GGFK01005043    MBW38364.1    GBXI01016784    JAC97507.1    ATLV01023390    KE525342    KFB49058.1    CH954177    EDV58633.1    U60298    U62547    AE014134    AY089226    BT044244    OUUW01000006    SPP82538.1    CH479195    EDW27333.1    CM000157    EDW88356.1    CH963920    EDW77737.2    CH379061    EAL33303.3    CM002910    KMY90165.1    CH480818    EDW51682.1    CH902620    EDV32004.2    GBYB01007807    JAG77574.1    UFQS01000392    UFQT01000392    SSX03542.1    SSX23907.1    AXCN02001308    APGK01046987    APGK01046988    APGK01046989    KB741073    ENN74383.1    CH916368    EDW03213.1    KZ288192    PBC34303.1    ADMH02000150    ETN67597.1    KB632431    ERL95502.1    JXJN01009687    CH933807    EDW13493.1    CH940654    EDW57540.1    KQ435850    KOX70898.1    KPU73732.1    CCAG010008798    KQ414572    KOC71332.1    JXJN01015380    GEDC01018080    JAS19218.1    NEVH01026401    PNF14201.1    GL448228    EFN85052.1    LJIG01022779    KRT78759.1    GECU01016954    GECU01003061    JAS90752.1    JAT04646.1    GBHO01030342    JAG13262.1    GDHC01006809    JAQ11820.1    ABLF02039531    KK854446    PTY15917.1    GEFM01003947    JAP71849.1    ABJB010228526    DS949314    EEC18857.1    DS235172    EEB12979.1    GECL01002696    JAP03428.1    GBGD01000281    JAC88608.1    ACPB03005539    GFTR01008473    JAW07953.1    GEDV01005598    JAP82959.1    PNF14200.1    PNF14199.1    GFAC01002090    JAT97098.1    JARO02005098    KPP67359.1   

Proteomes

UP000005204    UP000218220    UP000283053    UP000053240    UP000007151    UP000053268    + More
UP000069940    UP000249989    UP000027135    UP000075903    UP000007062    UP000245037    UP000076407    UP000183832    UP000075840    UP000092461    UP000075902    UP000036403    UP000002320    UP000076408    UP000005205    UP000078492    UP000075883    UP000075920    UP000075901    UP000075881    UP000007266    UP000000311    UP000069272    UP000075900    UP000075884    UP000053097    UP000279307    UP000007755    UP000192221    UP000075885    UP000030765    UP000008711    UP000000803    UP000075880    UP000268350    UP000008744    UP000002282    UP000007798    UP000078200    UP000001819    UP000095301    UP000001292    UP000007801    UP000075886    UP000019118    UP000001070    UP000242457    UP000000673    UP000030742    UP000092460    UP000009192    UP000008792    UP000053105    UP000095300    UP000092444    UP000053825    UP000092445    UP000005203    UP000235965    UP000008237    UP000007819    UP000001555    UP000009046    UP000015103    UP000034805   

PRIDE

H9JIK9    Q27607   

Pfam

PF18136   DNApol_Exo        + More
PF00928   Adap_comp_sub
PF01217   Clat_adaptor_s
PF00476   DNA_pol_A
PF01176   eIF-1a
PF03129   HGTP_anticodon
PF02686   Glu-tRNAGln
PF01612   DNA_pol_A_exo1

Interpro

IPR011012   Longin-like_dom_sf        + More
IPR002297   DNA-dir_DNA_pol_A_mt       
IPR028565   MHD       
IPR018240   Clathrin_mu_CS       
IPR012337   RNaseH-like_sf       
IPR001098   DNA-dir_DNA_pol_A_palm_dom       
IPR001392   Clathrin_mu       
IPR022775   AP_mu_sigma_su       
IPR036168   AP2_Mu_C_sf       
IPR041336   DNApol_Exo       
IPR019760   DNA-dir_DNA_pol_A_CS       
IPR006196   RNA-binding_domain_S1_IF1       
IPR001253   TIF_eIF-1A       
IPR012340   NA-bd_OB-fold       
IPR036621   Anticodon-bd_dom_sf       
IPR004154   Anticodon-bd       
IPR003837   Asp/Glu-ADT_csu       
IPR036113   Asp/Glu-ADT_sf_sub_c       
IPR002562   3'-5'_exonuclease_dom       

SUPFAM

SSF53098   SSF53098        + More
SSF49447   SSF49447       
SSF64356   SSF64356       
SSF50249   SSF50249       
SSF141000   SSF141000       

Gene 3D

3.40.50.800     1.10.20.60    

ProteinModelPortal

H9JIK9   A0A2W1BGU1   A0A2A4JVT1   A0A2H1VVJ9   A0A437B739   A0A0N1PHG4   + More
A0A212EKX6   A0A194QD71   A0A182HFD6   A0A067QMN5   A0A182US34   Q7QET4   A0A1S4G836   A0A2Z5U602   A0A2P8YGW7   A0A182XQ05   A0A1J1IL40   A0A2C9GQN8   A0A1B0EW77   A0A182TD53   A0A0J7KY38   B0WYY9   A0A182YK54   A0A158P1B2   A0A151J3N1   A0A182MJW0   A0A182W543   A0A182SA91   A0A182K7I2   D2A2U3   A0A034W6H6   E2A680   A0A182FJT7   A0A1Y1KQB2   A0A1Q3EWH2   E9IKY1   A0A182RCF6   A0A1Y1KTR5   A0A182N1E0   A0A026W4E4   F4W9B2   A0A0K8V1M3   A0A1W4UUS9   A0A2M4AC42   A0A0A1WGH5   A0A182PVA5   A0A084WFR4   B3NAD1   Q27607   A0A182ILV4   A0A3B0KFX3   B4GX06   B4P3Q5   B4N0X4   A0A1A9V9J6   Q29K74   A0A0J9TMZ7   A0A1I8MKW9   B4HXA2   B3MN26   A0A0C9R4X0   A0A336KHX2   A0A182QTQ9   N6T9G9   B4JCX4   A0A2A3ER84   W5JT93   U4UQW6   A0A1B0B7M3   B4KIY0   B4M8L4   A0A0N0BDZ2   A0A0N8NZ99   A0A1I8Q0N8   A0A1B0FDD3   A0A0L7RK58   A0A1B0BJF8   A0A1B0A530   A0A088AV73   A0A1B6D0L6   A0A2J7PCY6   E2BGS0   A0A0T6AVN1   A0A1B6JZM3   A0A0A9WXR6   A0A146LZN8   X1WIQ6   A0A2R7W845   A0A131XXV7   B7QJ35   E0VHX3   A0A0V0G5V2   A0A069DX00   T1HWN3   A0A224XIJ0   A0A131YUP9   A0A2J7PCY7   A0A2J7PCY9   A0A1E1XD11   A0A0P7WZF3  

PDB

5C53     E-value=2.04927e-115,     Score=1066

KEGG

101736791    K02332 DNA polymerase gamma 1 [EC:2.7.7.7] | (RefSeq) DNA polymerase subunit gamma-1, mitochondrial

GO

GO:0005760   GO:0016192   GO:0003887   GO:0003677   GO:0006260   GO:0030131   GO:0006886   GO:0005739   GO:0006264   GO:0008408   GO:0003743   GO:0016021   GO:0032543   GO:0050567   GO:0005524   GO:0030956   GO:0006450   GO:0070681   GO:0019896   GO:1904115   GO:0006261   GO:0007562   GO:0008340   GO:0003676   GO:0019012   GO:0051603   GO:0030145   GO:0006412   GO:0016876   GO:0043039  

PANTHER

PTHR10267  

Subcellular location

Mitochondrion  

Length:

671

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.01717

Exp number, first 60 AAs:

0

Total prob of N-in:

0.00051

outside

1  -  671

 

 

Pi

310.595922

Theta

174.615002

Tajima's D

2.143733

CLR

0.237144

CSRT

0.903854807259637

Interpretation

Uncertain