Gene

KWMTBOMO08500  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA009342

Annotation

PREDICTED:_GPI_mannosyltransferase_3_[Amyelois_transitella]

Location

Bomo_Chr14(-):10926280-10934833      View in EpiBrowser

Full name

Mannosyltransferase       + More
GPI mannosyltransferase 3      
Succinate--CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial      

Alternative Name

GPI mannosyltransferase III
Phosphatidylinositol-glycan biosynthesis class B protein
Succinyl-CoA synthetase subunit alpha

Location in the cell

PlasmaMembrane   Reliability : 4.55

CDS

ATGGTGTTGGCAAAGGGTTTGAGGCCCGTTCAGGTGGTCTCTTTTATATTATTCGTGAGAATACTTTCTGTATTCTTAGTGAGAACTTGGTATGTTCCTGACGAGTACTGGCAGACCTTGGAAGTGGCCCATAAACAAGTGTTCCACTATGGAGAACTCACCTGGGAATGGCAGAGGGGTATCAGGAGCTACTTATTCCCGAGCGTAGTTGCTGTCTTGTATAGTATATTGAAGGTTACTGGCTTGGATCACCCTGAAGCTGTGATTCTTCTACCTAGGATCCTACAAGCAATACTCAGTACTGCAGCAGACTACAGTTTCTATAAATGGACAGGAGGTCGGAAGTGGGCTCTGTTCCTCATTCTCACATCTTGGTTCTGGTTCTATACTTCAGGCAGAACACTGCTCCAGACTACAGAGACAGCTCTTGTGGCAATCGCCTTATCTGTTTTTCCATTCAAGAGTGGAAAACTTGGATATTATGATAAAGAGAACACATCATGGATATGGCTAGCAGTCATAGCTGTGTTTCTTCGGCCAACTTCTGCACCTCTATGGGCAGTGCTCACTATCTACAACTTACTGACAACGAACCAGTCCAAGTTGCAGTTACTGTTGAGATCATATTTACCTATTGGATTGATCGCTGGTAGTGCTTTAGTGGGTTTAGATACATACTTCTACGGAAGAGTTGTGATCACTCCATGGGAATTCTTCAAGTTTAATGTGCTTTATGATATTGCATCTTTCTATGGAAAACATCCATGGCATTGGTACTTGAGCCAGGGTCTGCCGGCCGTGCTGGGCGTCAACACAGTGCCGGTGCTGTGGGCAGCGGTCAACATAGTTCGTCGACCCCGGGAGAACAAGACGGGGGCGCTGCTGTTGTTTGCTGCAGCTCTACACATAGCACTATATAGCTTCATCTCGCACAAGGAGTTCAGATTCGTATTACCGTTACTGCCCATCCTGCTGTACCTCGCGCAGGACGCCATCGTACCGTGGAGCAGGAAAGCTAAAAGATGGCAGCTGTACGTGGTGGCGGCGTGCATGGTGGTGGGCAACGCGCTGCCGGCCGCGTACTTCGGCGCCGTGCACCAGAGGGGCGCGCTGGACGTGATGCCGCTGCTGCGGGACGCCGCCTCCACCAACCGCTCCTCCATCGCCTTCCTCATGCCCTGCCACTCGACGCCGCTCTATAGCCATATCCACAAAAACATCACAACCCGTTATCTCCACTGTGAGCCTCCGTTCAACAAGCCTGGCGAAACTTACGAATCGGAAGCCTTCTTCAACAACCCTCTCCGGTGGTGGAGGAATGAGTACTCCAGTAGACAGACACCAACTCTGGTCGTACTCTTCGATACCCTTAAAGGGAGAGTAGAAAATATTCTGAGTGGATATCGGTTGTTGCATGAGATTCCTCATACACAGTTCCCAGAAGGAGAGGTCGGTGAGAATGTCTTGGTGTATCAGAAAATAGAAAGTCAACCTAAACCGCCGGCTGACGAAGTTGTTTAA

Protein

MVLAKGLRPVQVVSFILFVRILSVFLVRTWYVPDEYWQTLEVAHKQVFHYGELTWEWQRGIRSYLFPSVVAVLYSILKVTGLDHPEAVILLPRILQAILSTAADYSFYKWTGGRKWALFLILTSWFWFYTSGRTLLQTTETALVAIALSVFPFKSGKLGYYDKENTSWIWLAVIAVFLRPTSAPLWAVLTIYNLLTTNQSKLQLLLRSYLPIGLIAGSALVGLDTYFYGRVVITPWEFFKFNVLYDIASFYGKHPWHWYLSQGLPAVLGVNTVPVLWAAVNIVRRPRENKTGALLLFAAALHIALYSFISHKEFRFVLPLLPILLYLAQDAIVPWSRKAKRWQLYVVAACMVVGNALPAAYFGAVHQRGALDVMPLLRDAASTNRSSIAFLMPCHSTPLYSHIHKNITTRYLHCEPPFNKPGETYESEAFFNNPLRWWRNEYSSRQTPTLVVLFDTLKGRVENILSGYRLLHEIPHTQFPEGEVGENVLVYQKIESQPKPPADEVV

Description

Mannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers the third alpha-1,2-mannose to Man2-GlcN-acyl-PI during GPI precursor assembly (By similarity).
Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The alpha subunit of the enzyme binds the substrates coenzyme A and phosphate, while succinate binding and specificity for either ATP or GTP is provided by different beta subunits.

Catalytic Activity

ATP + CoA + succinate = ADP + phosphate + succinyl-CoA
CoA + GTP + succinate = GDP + phosphate + succinyl-CoA

Subunit

Heterodimer of an alpha and a beta subunit. Different beta subunits determine nucleotide specificity. Together with an ATP-specific beta subunit, forms an ADP-forming succinyl-CoA synthetase (A-SCS). Together with a GTP-specific beta subunit forms a GDP-forming succinyl-CoA synthetase (G-SCS).

Similarity

Belongs to the glycosyltransferase 22 family.
Belongs to the glycosyltransferase 22 family. PIGB subfamily.
Belongs to the succinate/malate CoA ligase alpha subunit family.

Keywords

Alternative splicing   Complete proteome   Endoplasmic reticulum   Glycoprotein   Glycosyltransferase   GPI-anchor biosynthesis   Membrane   Reference proteome   Transferase   Transmembrane   Transmembrane helix  

Feature

chain  Mannosyltransferase
splice variant  In isoform B.

Uniprot

H9JIJ2   A0A2A4JIR7   A0A2W1B923   A0A2H1VCT5   A0A194Q788   A0A212EKY4   + More
A0A437B735   B4N589   A0A1W4W3Z6   B4HTU5   B3NBZ5   A0A0J9RNI0   B4PH62   B4IZW1   B3M806   B4KYA0   Q9VZM5-2   B4LDG7   A0A1J1ILD4   A0A0J9RNZ5   D6WD71   A0A0Q5UI06   A0A0R1E0S1   A0A1W4VSL4   A0A0P8XU98   A0A182GC61   A0A336MML2   A0A336MH85   Q2LZU0   A0A3B0JS25   B0X524   A0A023EUF1   A0A182M423   A0A0Q9XMV0   A0A0Q9XNI0   A0A1I8M585   A0A0K8VE28   Q9VZM5   A0A0A1X6J3   A0A0A1WQE4   W8C1D2   A0A1Q3FML2   A0A0R3P861   A0A1W4XBQ4   A0A0Q9WHY3   A0A182JVZ1   A0A182XXE7   A0A340TB88   A0A1Y9J0T6   Q7QFX4   A0A182P793   A0A1I8P309   A0A182LAQ6   A0A182SM66   A0A182I8U0   A0A1I8M584   A0A0L0CCX1   Q0C786   A0A1S4EUN4   A0A182R5W5   A0A182PZW1   A0A182N4E5   A0A182UQ54   A0A084VDC9   A0A1B0AJC0   A0A1B0BAR7   A0A1A9XJY7   A0A182FSD8   A0A1B0GNQ9   A0A1Y1NEB8   W5JB23   A0A1I8P354   A0A1B0C863   A0A1B0GF61   A0A1A9US15   B4QPY6   A0A0M4EIX9   B4H818   A0A182JMN6   A0A182UC57   A0A1Q3FLJ7   U4U3H7   A0A1A8H8T8   A0A1A8DLM9   A0A1A8JYL1   E9JD33   A0A1A8A0E2   A0A1A8PRD7   A0A1A8NJD5   A0A2A3EMT0   A0A2I4CTF0   A0A1W4XBH1   A7RSA4   Q7SXZ1   A0A0R4IFP6   W4XVD5  

EC Number

2.4.1.-
6.2.1.4
6.2.1.5

Pubmed

19121390    28756777    26354079    22118469    17994087    22936249    + More
17550304    10731132    12537572    18362917    19820115    26483478    15632085    24945155    18057021    25315136    25830018    24495485    25244985    12364791    14747013    17210077    20966253    26108605    17510324    24438588    28004739    20920257    23761445    23537049    21282665    17615350    23594743   

EMBL

BABH01027083    BABH01027084    NWSH01001280    PCG71861.1    KZ150210    PZC72209.1    + More
ODYU01001865    SOQ38660.1    KQ459337    KPJ01407.1    AGBW02014173    OWR42139.1    RSAL01000133    RVE46326.1    CH964101    EDW79528.1    CH480817    EDW50366.1    CH954178    EDV50812.1    CM002912    KMY97423.1    CM000159    EDW93299.1    CH916366    EDV96733.1    CH902618    EDV39914.1    CH933809    EDW17679.1    AE014296    BT003240    CH940647    EDW68905.1    CVRI01000050    CRK99265.1    KMY97422.1    KQ971312    EEZ98306.1    KQS43526.1    KRK01092.1    KPU78291.1    JXUM01053700    JXUM01053701    JXUM01053702    JXUM01053703    KQ561787    KXJ77539.1    UFQT01001210    SSX29517.1    UFQT01001263    SSX29752.1    CH379069    EAL29416.2    OUUW01000009    SPP84917.1    DS232362    EDS40670.1    GAPW01001026    JAC12572.1    AXCM01002368    KRG05687.1    KRG05688.1    GDHF01015212    GDHF01001253    JAI37102.1    JAI51061.1    GBXI01007957    JAD06335.1    GBXI01013023    JAD01269.1    GAMC01007015    GAMC01007014    JAB99541.1    GFDL01006283    JAV28762.1    KRT07955.1    KRF84067.1    AAAB01008844    EAA06057.4    APCN01003119    JRES01000577    KNC30095.1    CH477186    EAT48940.1    AXCN02000039    ATLV01011259    KE524659    KFB35973.1    JXJN01011133    AJVK01029700    GEZM01005566    JAV96089.1    ADMH02001999    ETN60045.1    AJWK01000216    AJWK01000217    CCAG010012763    CM000363    EDX09106.1    CP012525    ALC43901.1    CH479220    EDW34812.1    GFDL01006643    JAV28402.1    KB631874    ERL86863.1    HAEC01010909    SBQ79125.1    HAEA01005958    SBQ34438.1    HAEE01005071    SBR25091.1    GL771866    EFZ09140.1    HADY01009501    HAEJ01004928    SBP47986.1    HAEH01008262    SBR84000.1    HAEG01003284    SBR68927.1    KZ288206    PBC33065.1    DS469533    EDO45709.1    BC055191    CABZ01069901    CU627990    AAGJ04037456    AAGJ04037457   

Proteomes

UP000005204    UP000218220    UP000053268    UP000007151    UP000283053    UP000007798    + More
UP000192221    UP000001292    UP000008711    UP000002282    UP000001070    UP000007801    UP000009192    UP000000803    UP000008792    UP000183832    UP000007266    UP000069940    UP000249989    UP000001819    UP000268350    UP000002320    UP000075883    UP000095301    UP000192223    UP000075881    UP000076408    UP000075920    UP000076407    UP000007062    UP000075885    UP000095300    UP000075882    UP000075901    UP000075840    UP000037069    UP000008820    UP000075900    UP000075886    UP000075884    UP000075903    UP000030765    UP000092445    UP000092460    UP000092443    UP000069272    UP000092462    UP000000673    UP000092461    UP000092444    UP000078200    UP000000304    UP000092553    UP000008744    UP000075880    UP000075902    UP000030742    UP000242457    UP000192220    UP000001593    UP000000437    UP000007110   

PRIDE

H9JIJ2    Q9VZM5   

Pfam

PF03901   Glyco_transf_22        + More
PF08423   Rad51
PF00549   Ligase_CoA
PF02629   CoA_binding
PF09457   RBD-FIP

Interpro

IPR039521   PIG-B/GPI10        + More
IPR005599   GPI_mannosylTrfase       
IPR027417   P-loop_NTPase       
IPR013632   DNA_recomb/repair_Rad51_C       
IPR020588   RecA_ATP-bd       
IPR003593   AAA+_ATPase       
IPR036291   NAD(P)-bd_dom_sf       
IPR005811   CoA_ligase       
IPR003781   CoA-bd       
IPR005810   CoA_lig_alpha       
IPR033847   Citrt_syn/SCS-alpha_CS       
IPR017440   Cit_synth/succinyl-CoA_lig_AS       
IPR016102   Succinyl-CoA_synth-like       
IPR019018   Rab-bd_FIP-RBD       
IPR037245   FIP-RBD_C_sf       

SUPFAM

SSF52540   SSF52540        + More
SSF51735   SSF51735       
SSF52210   SSF52210       
SSF144270   SSF144270       

Gene 3D

3.40.50.261    

ProteinModelPortal

H9JIJ2   A0A2A4JIR7   A0A2W1B923   A0A2H1VCT5   A0A194Q788   A0A212EKY4   + More
A0A437B735   B4N589   A0A1W4W3Z6   B4HTU5   B3NBZ5   A0A0J9RNI0   B4PH62   B4IZW1   B3M806   B4KYA0   Q9VZM5-2   B4LDG7   A0A1J1ILD4   A0A0J9RNZ5   D6WD71   A0A0Q5UI06   A0A0R1E0S1   A0A1W4VSL4   A0A0P8XU98   A0A182GC61   A0A336MML2   A0A336MH85   Q2LZU0   A0A3B0JS25   B0X524   A0A023EUF1   A0A182M423   A0A0Q9XMV0   A0A0Q9XNI0   A0A1I8M585   A0A0K8VE28   Q9VZM5   A0A0A1X6J3   A0A0A1WQE4   W8C1D2   A0A1Q3FML2   A0A0R3P861   A0A1W4XBQ4   A0A0Q9WHY3   A0A182JVZ1   A0A182XXE7   A0A340TB88   A0A1Y9J0T6   Q7QFX4   A0A182P793   A0A1I8P309   A0A182LAQ6   A0A182SM66   A0A182I8U0   A0A1I8M584   A0A0L0CCX1   Q0C786   A0A1S4EUN4   A0A182R5W5   A0A182PZW1   A0A182N4E5   A0A182UQ54   A0A084VDC9   A0A1B0AJC0   A0A1B0BAR7   A0A1A9XJY7   A0A182FSD8   A0A1B0GNQ9   A0A1Y1NEB8   W5JB23   A0A1I8P354   A0A1B0C863   A0A1B0GF61   A0A1A9US15   B4QPY6   A0A0M4EIX9   B4H818   A0A182JMN6   A0A182UC57   A0A1Q3FLJ7   U4U3H7   A0A1A8H8T8   A0A1A8DLM9   A0A1A8JYL1   E9JD33   A0A1A8A0E2   A0A1A8PRD7   A0A1A8NJD5   A0A2A3EMT0   A0A2I4CTF0   A0A1W4XBH1   A7RSA4   Q7SXZ1   A0A0R4IFP6   W4XVD5  

KEGG

101736651    K05286 GPI mannosyltransferase 3 [EC:2.4.1.-] | (RefSeq) GPI mannosyltransferase 3

PATHWAY

00563    Glycosylphosphatidylinositol (GPI)-anchor biosynthesis - Bombyx mori (domestic silkworm)
01100    Metabolic pathways - Bombyx mori (domestic silkworm)

GO

GO:0005789   GO:0016021   GO:0006506   GO:0000030   GO:0005783   GO:0005524   GO:0006281   GO:0008094   GO:0003677   GO:0004776   GO:0004775   GO:0000166   GO:0005739   GO:0016757   GO:0048037   GO:0006099   GO:0005634   GO:0007067   GO:0006508   GO:0019012   GO:0051603   GO:0030145   GO:0006412   GO:0016876   GO:0043039  

PANTHER

PTHR22760   PTHR22760:SF4  

Subcellular location

Endoplasmic reticulum membrane  
Mitochondrion  

Length:

506

Number of predicted TMHs:

8

Exp number of AAs in TMHs:

182.4978

Exp number, first 60 AAs:

21.35114

Total prob of N-in:

0.37411

POSSIBLE N-term signal

sequence

outside

1  -  9

TMhelix

10  -  32

inside

33  -  115

TMhelix

116  -  135

outside

136  -  166

TMhelix

167  -  189

inside

190  -  209

TMhelix

210  -  232

outside

233  -  257

TMhelix

258  -  280

inside

281  -  291

TMhelix

292  -  309

outside

310  -  313

TMhelix

314  -  336

inside

337  -  342

TMhelix

343  -  365

outside

366  -  506

 

 

Pi

200.827968

Theta

201.268967

Tajima's D

0.475086

CLR

0.20644

CSRT

0.510724463776811

Interpretation

Uncertain