Gene

KWMTBOMO08478

Pre Gene Modal

BGIBMGA009349

Annotation

polymerase_[Lishi_Spider_Virus_1]

Location

Bomo_Chr14(+):10584517-10585443      View in EpiBrowser

Full name

Large structural protein       + More
RNA-directed RNA polymerase L      

Alternative Name

RNA-directed RNA polymerase L
Large structural protein
Replicase
Transcriptase

Location in the cell

Nuclear   Reliability : 2.383

CDS

ATGTGTGCGCATTCAAAGCCAATATGTCCCATCTTCGAGAGTCAAAGAACGGTAGCACTAGTCTCTCCAGAATCAATTGTCACCGAACGTAAGTTTGATACAGCAATTAGGCTATCACAAGTTGTCTCCTTTAAGCGGAGGCTAGAAGAAGGTCAATCCACATTGGATGATAAAATCTTGATAGAAACCTTGAGGAGACAGGGTGTCAAGGCACAAGATCTGATAATTGACCATGCTGTGTATTCCAGGCTGTTACAAGAAGTTCTAGCATCTGAAACCCCAGCCAGGAAAATCAACTCTAAAGAGAATGACCAGATCAGTCAAACATGGTCACTAATACAGAGGAATCTTACGCTACAAATAGGTTATATGACTGAACAAAACACACTGCCGGAACATGAAAGTACAAAAGCAGGATTACATGAATTCTTCTCTTCTAAAATGCCTGATAACACGTTGAGAAGACTAGTACTTCTGGCAACAAGATTGGCAGTTATGGTCGAGAAGATGGGATTACTCCAGTCTGTCAGGTCAGCAGGAAAATCCATTGAGACATTTCTAGACGAGGCATATAAATGTAACAGGTGGGATGATCAAGCATTTAATATTAAGACCCTGTGGTGCAATCGGCTATGTGCAATTAATCTAGGATCAGACCAATGGTATATAATGCCCAGACCATATTTACTTATGATGCACAATAAAGTGTCAGATCTTATATCAGTATTGGTATGCAGCAACTCTATGTCAGGAGTGTCAGGCGATAGTAACCTATACAATACTTGTATTGAACTGTAA

Protein

MCAHSKPICPIFESQRTVALVSPESIVTERKFDTAIRLSQVVSFKRRLEEGQSTLDDKILIETLRRQGVKAQDLIIDHAVYSRLLQEVLASETPARKINSKENDQISQTWSLIQRNLTLQIGYMTEQNTLPEHESTKAGLHEFFSSKMPDNTLRRLVLLATRLAVMVEKMGLLQSVRSAGKSIETFLDEAYKCNRWDDQAFNIKTLWCNRLCAINLGSDQWYIMPRPYLLMMHNKVSDLISVLVCSNSMSGVSGDSNLYNTCIEL

Description

RNA-directed RNA polymerase that catalyzes the transcription of viral mRNAs, their capping and polyadenylation. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). The viral polymerase binds to the genomic RNA at the 3' leader promoter, and transcribes subsequently all viral mRNAs with a decreasing efficiency. The first gene is the most transcribed, and the last the least transcribed. The viral phosphoprotein acts as a processivity factor. Capping is concommitant with initiation of mRNA transcription. Indeed, a GDP polyribonucleotidyl transferase (PRNTase) adds the cap structure when the nascent RNA chain length has reached few nucleotides. Ribose 2'-O methylation of viral mRNA cap precedes and facilitates subsequent guanine-N-7 methylation, both activities being carried by the viral polymerase. Polyadenylation of mRNAs occur by a stuttering mechanism at a slipery stop site present at the end viral genes. After finishing transcription of a mRNA, the polymerase can resume transcription of the downstream gene.

Catalytic Activity

a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)
a 5'-(5'-triphosphoguanosine)-(purine-ribonucleotide)-[mRNA] + S-adenosyl-L-methionine = a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(purine-ribonucleotide)-[mRNA] + S-adenosyl-L-homocysteine
a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-purine-ribonucleotide)-(ribonucleotide)-[mRNA] + S-adenosyl-L-methionine = a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-purine-ribonucleotide)-(2'-O-methyl-ribonucleotide)-[mRNA] + H(+) + S-adenosyl-L-homocysteine
a 5'-triphospho-(purine-ribonucleotide)-[mRNA] + GDP + H(+) = a 5'-(5'-triphosphoguanosine)-(purine-ribonucleotide)-[mRNA] + diphosphate

Similarity

Belongs to the paramyxovirus L protein family.

Uniprot

H9JIJ9   A0A1L3KN74   A0A0B5KRA0   A0A0B5KJY4   A0A1L3KN70   A0A0B5KTA1   + More
A0A221LFF1   A0A1L3KN44   A0A346M252   A0A0S2RRG3   A0A346M266   A0A1L3KMX9   A0A1L4A1T3   A0A0B5KR88   A0A0B5KX99   A0A0B5KEQ8   A0A0B5KT91   A0A0B5KEM5   A0A3G3BTF8   A0A3G3BTR3   A0A221LFF6   A0A172MHM9   A0A1L3KMW6   A0A0B5KX86   A0A0B5KTB0   A0A0B5KJX3   A0A075EJ04   A0A076E9Z7   A0A0A1G6U7   A0A1L3KN64   A0A1L3KMQ9   A0A2P1GMT0   A0A2P1GMQ1   A0A1L3KMY9  

Pubmed

19121390    27880757    25633976    30098450    26823588    25056893    + More
29618816   

EMBL

BABH01027048    KX884453    APG78828.1    KM817597    AJG39051.1    KM817612    + More
AJG39080.1    KX884451    APG78824.1    KM817604    AJG39067.1    MF189989    ASM94010.1    KX884455    APG78831.1    MH188031    AXQ04827.1    KU095839    ALP32028.1    MH188036    AXQ04841.1    KX884424    APG78716.1    KX924630    API61887.1    KM817593    MH688555    AJG39041.1    QBQ65118.1    KM817609    AJG39073.1    KM817610    AJG39074.1    KM817599    AJG39057.1    KM817594    AJG39044.1    MK026591    AYP67566.1    MK026566    AYP67535.1    MF189987    ASM94008.1    KU230451    ANC97697.1    KX884427    APG78724.1    KM817600    AJG39058.1    KM817611    AJG39077.1    KM817606    AJG39070.1    KJ746903    AIE42676.2    KM048317    AII01805.1    KM460042    AIY53910.1    KX884449    APG78818.1    KX884419    APG78700.1    MG600011    AVM87278.1    MG600010    AVM87275.1    KX884439    APG78770.1   

Proteomes

UP000005204    UP000203961    UP000279202    UP000204621    UP000272305    UP000203655    + More
UP000201479    UP000202207    UP000204131    UP000276326    UP000204649    UP000207671    UP000214367    UP000202848    UP000202515    UP000201991    UP000277466    UP000218499    UP000204161    UP000202577    UP000202294   

PRIDE

H9JIJ9   

Pfam

PF00946   Mononeg_RNA_pol        + More
PF14318   Mononeg_mRNAcap
PF14314   Methyltrans_Mon
PF01728   FtsJ

Interpro

IPR014023   Mononeg_RNA_pol_cat        + More
IPR026890   Mononeg_mRNAcap       
IPR039530   L_methyltransferase_rhabdo       
IPR025786   Mononega_L_MeTrfase       
IPR016269   RNA-dir_pol_paramyxovirus       
IPR002877   rRNA_MeTrfase_FtsJ_dom       

ProteinModelPortal

H9JIJ9   A0A1L3KN74   A0A0B5KRA0   A0A0B5KJY4   A0A1L3KN70   A0A0B5KTA1   + More
A0A221LFF1   A0A1L3KN44   A0A346M252   A0A0S2RRG3   A0A346M266   A0A1L3KMX9   A0A1L4A1T3   A0A0B5KR88   A0A0B5KX99   A0A0B5KEQ8   A0A0B5KT91   A0A0B5KEM5   A0A3G3BTF8   A0A3G3BTR3   A0A221LFF6   A0A172MHM9   A0A1L3KMW6   A0A0B5KX86   A0A0B5KTB0   A0A0B5KJX3   A0A075EJ04   A0A076E9Z7   A0A0A1G6U7   A0A1L3KN64   A0A1L3KMQ9   A0A2P1GMT0   A0A2P1GMQ1   A0A1L3KMY9  

KEGG

NA   

GO

GO:0003968   GO:0004482   GO:0019012   GO:0005524   GO:0016021   GO:0030430  

Subcellular location

Virion  
Host cytoplasm  

Length:

265

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.03406

Exp number, first 60 AAs:

0

Total prob of N-in:

0.04011

outside

1  -  265

 

 

Pi

314.321306

Theta

203.018601

Tajima's D

1.625628

CLR

0

CSRT

0.818059097045148

Interpretation

Uncertain