Gene

KWMTBOMO08477

Annotation

polymerase_[Lishi_Spider_Virus_1]

Location

Bomo_Chr14(-):10582554-10584136      View in EpiBrowser

Full name

Large structural protein       + More
RNA-directed RNA polymerase L      

Alternative Name

RNA-directed RNA polymerase L
Large structural protein
Replicase
Transcriptase

Location in the cell

Mitochondrial   Reliability : 1.786 Nuclear   Reliability : 1.758

CDS

ATGCCAGCGTTACCGTCTACAGTGTTGAGAAATAAGATGGAAACATTATTGCCTAAAATCACTAAGAATCAAGACGTCAAGAATCTGATTCAGGCTGCTAAATCAGTGTCCAGTCAGGAGTTCATTAAGGCCTTAGATTCATCTAATATATTACCTGCTCGTGTATTTGCTGCTCTATATGCAGCAAGTCCTAAAGGGATGTTGTCGGAATTTCTCGCAAAGTTTGAAACAGCAAGAAGTGTGTTACAAGCACTTATCTTAGGAAGCGGAAAGTATGCGGCTAGAAAAATGCTAAGACGACTAGTTAAACAGGAACACATTTTACAGAACTGGAGAGTCAATGTTATAAAAGGTCATGTTCGTGGTTACGATTATATGCATATTATCCTAGGTAACAAGTGCCCAGGAAAGGCCGCGAACTTAATTAGGGAAGAGTCATGGCGAAAACCTGTTGAGACAGTCACTATGCCTCCTCTTCAGCACCAAGTATATATGACAACACCTGAAAGAGGATCATTGAGTATGCATGATATCAAAAGTCATTTTACAATTAAATGTAACCAGTCCACAACTACCATTACCGAAGACGGGCGTCCTCACTACAGCACAGGGAATTTTAGACCTTTCGAGGGATACACTACCAGAACTGGTACCGTGGAGCCCACTATGAGTTTTATTGAGAAGGATGTTTTACTATCGAAACTGCAAAACGTCATAGAATTGGCTTCATGGACAAACAAGACTGGACTGAACAAAGAGAAGACTGCATCAATCAACAGTAATCTGCATCTGCTGATAGCTAAAATTGTTGAACAACACACTGATCATGACTTGAAGGTATTTGCACCATTCGTTGGCTGCCGACGATCTGGTACAGTTCAACATCATATCAGATGTCCAGGATACAGAGAGTCTATAGTCCCCAATACTCTACTAAATATGTATACACATACAGAGGGAGAGTCTGATTCCAACATGCATGTGAACTCCCAGCCTGGACAACATTACAAGATTAATTTCCTACACATCTACTGTTATGCTGTAGTTAGCGTCTTCTTGCACAATGAGATTTATTGTACGCCATCAGACAATAAAATCGTATGGTGTGTAACCCGGGATTGTGAGGAGTGTATGAGCCCTATTGATAAGACCCCGATTGTCATAGATGAGTCACTAATTGCAGATATAGATTTCTCGGCATTGAAGATCACTACATTAACAGAAGATGCGATGGATGTACTCAGGAAATCATTGGATATTCATGAAGGACGTGTCTATATGGTACCTGATGAAGAGACTAGGCTAGAAAATCAAATTGCATGTGAGGGAGTAATCCAAGAATTGTTGGAGACATTAGTGATGTCTAAAAATGCCTTGATTACACGATACACTCAGCATGCAATGACAAATGAAGGAAAACAAGTGTTAGGGGCGTTTGCAGTGAAGAATAAAAGGAGAGAGATTGGATTATCGGAATAA

Protein

MPALPSTVLRNKMETLLPKITKNQDVKNLIQAAKSVSSQEFIKALDSSNILPARVFAALYAASPKGMLSEFLAKFETARSVLQALILGSGKYAARKMLRRLVKQEHILQNWRVNVIKGHVRGYDYMHIILGNKCPGKAANLIREESWRKPVETVTMPPLQHQVYMTTPERGSLSMHDIKSHFTIKCNQSTTTITEDGRPHYSTGNFRPFEGYTTRTGTVEPTMSFIEKDVLLSKLQNVIELASWTNKTGLNKEKTASINSNLHLLIAKIVEQHTDHDLKVFAPFVGCRRSGTVQHHIRCPGYRESIVPNTLLNMYTHTEGESDSNMHVNSQPGQHYKINFLHIYCYAVVSVFLHNEIYCTPSDNKIVWCVTRDCEECMSPIDKTPIVIDESLIADIDFSALKITTLTEDAMDVLRKSLDIHEGRVYMVPDEETRLENQIACEGVIQELLETLVMSKNALITRYTQHAMTNEGKQVLGAFAVKNKRREIGLSE

Description

RNA-directed RNA polymerase that catalyzes the transcription of viral mRNAs, their capping and polyadenylation. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). The viral polymerase binds to the genomic RNA at the 3' leader promoter, and transcribes subsequently all viral mRNAs with a decreasing efficiency. The first gene is the most transcribed, and the last the least transcribed. The viral phosphoprotein acts as a processivity factor. Capping is concommitant with initiation of mRNA transcription. Indeed, a GDP polyribonucleotidyl transferase (PRNTase) adds the cap structure when the nascent RNA chain length has reached few nucleotides. Ribose 2'-O methylation of viral mRNA cap precedes and facilitates subsequent guanine-N-7 methylation, both activities being carried by the viral polymerase. Polyadenylation of mRNAs occur by a stuttering mechanism at a slipery stop site present at the end viral genes. After finishing transcription of a mRNA, the polymerase can resume transcription of the downstream gene.

Catalytic Activity

a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)
a 5'-(5'-triphosphoguanosine)-(purine-ribonucleotide)-[mRNA] + S-adenosyl-L-methionine = a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(purine-ribonucleotide)-[mRNA] + S-adenosyl-L-homocysteine
a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-purine-ribonucleotide)-(ribonucleotide)-[mRNA] + S-adenosyl-L-methionine = a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-purine-ribonucleotide)-(2'-O-methyl-ribonucleotide)-[mRNA] + H(+) + S-adenosyl-L-homocysteine
a 5'-triphospho-(purine-ribonucleotide)-[mRNA] + GDP + H(+) = a 5'-(5'-triphosphoguanosine)-(purine-ribonucleotide)-[mRNA] + diphosphate

Similarity

Belongs to the paramyxovirus L protein family.

Uniprot

A0A1L3KMW6   A0A0B5KRA0   A0A1L4A1T3   A0A2K9YNF4   A0A2S0SZ96   A0A0B5KTA1   + More
A0A1L3KN70   A0A1L3KMX9   A0A1L3KN74   A0A221LFF1   A0A0B5KEQ8   A0A0S2RRG3   A0A1L3KN44   A0A346M252   A0A346M266   A0A0B5KJW3   A0A221LFF6   A0A2P1GMT0   A0A2P1GMQ1   A0A1L3KMY9   A0A2P1GMQ7   A0A0B5KX86   A0A1L3KMQ9   A0A0B5KT91   A0A0B5KT83   A0A0B5KEM5   A0A3G3BTF8   A0A1L3KN64   A0A385DMP2   A0A2U8JHB6   A0A0B5KR88   A0A076E9Z7   A0A0A1G6U7   A0A075EJ04   A0A0B5KX99   A0A3G4YIX7   A0A3G4YJ38   A0A3G4YJ66   A0A0B5KTB0   A0A172MHM9   A0A3G3BTR3   A0A0B5KJX3   A0A3G4YJ20   A0A1L3KN56   A0A1L3KMX3   A0A1L3KN37   A0A1L3KN90   A0A3G5FMI8   A0A1L3KMV0   A0A3B1EJ88   A0A1L3KML0   A0A2S1ZPS1   C4NFK9   C4NFL5   A0A2Z4HF79   A0A2Z4HFC4   A0A2Z4HFJ7   A0A2Z4HF97   A0A2Z4HF69   A0A2Z4HFG4   A0A2Z4GIN2   A0A2Z4HFS7   A0A2H4YI41   A0A2Z4HFJ1   A0A2Z4HFC6   A0A0B5KEN7   A0A2Z4HFA6   A0A2Z4HFC7   A0A2Z4HFB8   A0A2Z4HFG9   A0A1L3KMU5   A0A0B5KRF3   A0A060DA11   A0A1L3KN23   A0A0X3PRB1   A0A067YEZ6   A0A1Z2RTE0   A0A0X3PYN5   A0A1Z2RSU8   A0A1Z2RTB4   A0A1Z2RT54   A0A1Z2RT41   A0A2Z4Z3N4   A0A0B5KTH4   A0A1B1FIU1   A0A0B5KK67  

Pubmed

27880757    25633976    26823588    30098450    29618816    29728337    + More
25056893    30397237    30415391    29741476    19279111    29203920    24886057    24778199    28637756    29939128   

EMBL

KX884427    APG78724.1    KM817597    AJG39051.1    KX924630    API61887.1    + More
MF360789    AUW34382.1    MF416404    AWB14664.1    KM817604    AJG39067.1    KX884451    APG78824.1    KX884424    APG78716.1    KX884453    APG78828.1    MF189989    ASM94010.1    KM817610    AJG39074.1    KU095839    ALP32028.1    KX884455    APG78831.1    MH188031    AXQ04827.1    MH188036    AXQ04841.1    KM817601    AJG39060.1    MF189987    ASM94008.1    MG600011    AVM87278.1    MG600010    AVM87275.1    KX884439    APG78770.1    MG600009    AVM87272.1    KM817600    AJG39058.1    KX884419    APG78700.1    KM817599    AJG39057.1    KM817595    AJG39047.1    KM817594    AJG39044.1    MK026591    AYP67566.1    KX884449    APG78818.1    MG880117    AXQ59273.1    MG764515    AWK68105.1    KM817593    MH688555    AJG39041.1    QBQ65118.1    KM048317    AII01805.1    KM460042    AIY53910.1    KJ746903    AIE42676.2    KM817609    AJG39073.1    MH155923    AYV61054.1    MH155927    AYV61060.1    MH155920    AYV61047.1    KM817611    AJG39077.1    KU230451    ANC97697.1    MK026566    AYP67535.1    KM817606    AJG39070.1    MH155921    AYV61050.1    KX884458    APG78840.1    KX884409    APG78655.1    KX884444    APG78798.1    KX884461    APG78852.1    MH477287    AYW51538.1    KX884404    APG78635.1    MF287670    AWI42881.1    KX884408    APG78650.1    MH237595    AWK27462.1    FJ554525    ACQ94979.1    FJ554526    ACQ94985.1    MH430658    AWW13489.1    MH430666    AWW13507.1    MH430650    AWW13450.1    MH430653    AWW13465.1    MH430655    AWW13479.1    MH430648    MH430659    AWW13443.1    AWW13492.1    MH037149    AWW01093.1    MH430652    AWW13458.1    MG012486    AUE23903.1    MH430665    AWW13503.1    MH430657    AWW13485.1    KM817598    AJG39054.1    MH430663    MH430664    AWW13497.1    AWW13500.1    MH430656    AWW13482.1    MH430654    AWW13472.1    MH430651    AWW13453.1    KX884418    APG78697.1    KM817632    AJG39111.1    KF823814    AIB06812.1    KX884441    APG78782.1    GEEE01008921    JAP54304.1    KF530058    AHA90827.1    MF176375    ASA47494.1    GEEE01006270    JAP56955.1    MF176245    ASA47282.1    MF176356    ASA47464.1    MF176316    ASA47403.1    MF176296    ASA47369.1    MH213246    AXA52562.1    KM817641    AJG39142.1    KX257488    ANQ45640.1    KM817645    AJG39155.1   

Proteomes

UP000202515    UP000279202    UP000272305    UP000204621    UP000202207    UP000203961    + More
UP000204649    UP000201479    UP000203655    UP000280558    UP000202294    UP000201991    UP000202577    UP000207671    UP000202602    UP000214367    UP000204161    UP000204131    UP000218499    UP000276326    UP000202848    UP000277466    UP000201870    UP000204661    UP000201961    UP000203174    UP000203332    UP000279435    UP000119000    UP000029765    UP000201788    UP000204289    UP000232260    UP000113864    UP000202564    UP000127615    UP000204249    UP000278361   

Pfam

PF14318   Mononeg_mRNAcap        + More
PF00946   Mononeg_RNA_pol
PF01728   FtsJ
PF14314   Methyltrans_Mon

Interpro

IPR026890   Mononeg_mRNAcap        + More
IPR014023   Mononeg_RNA_pol_cat       
IPR016269   RNA-dir_pol_paramyxovirus       
IPR025786   Mononega_L_MeTrfase       
IPR002877   rRNA_MeTrfase_FtsJ_dom       
IPR039530   L_methyltransferase_rhabdo       
IPR039736   L_poly_C       

ProteinModelPortal

A0A1L3KMW6   A0A0B5KRA0   A0A1L4A1T3   A0A2K9YNF4   A0A2S0SZ96   A0A0B5KTA1   + More
A0A1L3KN70   A0A1L3KMX9   A0A1L3KN74   A0A221LFF1   A0A0B5KEQ8   A0A0S2RRG3   A0A1L3KN44   A0A346M252   A0A346M266   A0A0B5KJW3   A0A221LFF6   A0A2P1GMT0   A0A2P1GMQ1   A0A1L3KMY9   A0A2P1GMQ7   A0A0B5KX86   A0A1L3KMQ9   A0A0B5KT91   A0A0B5KT83   A0A0B5KEM5   A0A3G3BTF8   A0A1L3KN64   A0A385DMP2   A0A2U8JHB6   A0A0B5KR88   A0A076E9Z7   A0A0A1G6U7   A0A075EJ04   A0A0B5KX99   A0A3G4YIX7   A0A3G4YJ38   A0A3G4YJ66   A0A0B5KTB0   A0A172MHM9   A0A3G3BTR3   A0A0B5KJX3   A0A3G4YJ20   A0A1L3KN56   A0A1L3KMX3   A0A1L3KN37   A0A1L3KN90   A0A3G5FMI8   A0A1L3KMV0   A0A3B1EJ88   A0A1L3KML0   A0A2S1ZPS1   C4NFK9   C4NFL5   A0A2Z4HF79   A0A2Z4HFC4   A0A2Z4HFJ7   A0A2Z4HF97   A0A2Z4HF69   A0A2Z4HFG4   A0A2Z4GIN2   A0A2Z4HFS7   A0A2H4YI41   A0A2Z4HFJ1   A0A2Z4HFC6   A0A0B5KEN7   A0A2Z4HFA6   A0A2Z4HFC7   A0A2Z4HFB8   A0A2Z4HFG9   A0A1L3KMU5   A0A0B5KRF3   A0A060DA11   A0A1L3KN23   A0A0X3PRB1   A0A067YEZ6   A0A1Z2RTE0   A0A0X3PYN5   A0A1Z2RSU8   A0A1Z2RTB4   A0A1Z2RT54   A0A1Z2RT41   A0A2Z4Z3N4   A0A0B5KTH4   A0A1B1FIU1   A0A0B5KK67  

KEGG

NA   

GO

GO:0004482   GO:0003968   GO:0019012   GO:0005524   GO:0016021   GO:0006370   GO:0030430   GO:0051603   GO:0030145   GO:0006412   GO:0016876   GO:0043039  

Subcellular location

Virion  
Host cytoplasm  

Length:

492

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.0661299999999999

Exp number, first 60 AAs:

0.00289

Total prob of N-in:

0.00105

outside

1  -  492

 

 

Pi

230.627384

Theta

208.002971

Tajima's D

0.34314

CLR

249.860882

CSRT

0.463776811159442

Interpretation

Uncertain