SGID Silkworm Genome Informatics Database
Gene
KWMTBOMO08464  Validated by peptides from experiments
Pre Gene Modal
BGIBMGA009393
Annotation
FIB-L_protein_[Bombyx_mori]
Location
Bomo_Chr14(-):9685578-9698666      View in EpiBrowser
Full name
Fibroin light chain      
Alternative Name
L-fibroin
PG-1
Location in the cell
Extracellular   Reliability : 3.752
 

Sequence

CDS
ATGAAGCCTATATTTTTGGTATTACTCGTCGCTACAAGCGCCTACGCTGCACCATCGGTGACCATCAATCAATACAGTGATAATGAAATTCCACGCGACATTGATGATGGAAAAGCTAGTTCCGTAATCTCACGTGCATGGGACTACGTCGATGACACTGACAAAAGCATCGCCATCCTCAACGTTCAAGAGATCTTGAAGGACATGGCCAGCCAGGGCGATTATGCAAGTCAAGCATCAGCGGTGGCCCAAACCGCCGGAATTATCGCCCATCTATCTGCCGGTATCCCCGGTGATGCCTGTGCAGCCGCTAACGTCATTAACTCTTACACAGACGGCGTCAGGTCCGGAAACTTCGCCGGCTTCAGACAATCTCTCGGTCCCTTCTTCGGACACGTGGGACAAAACTTGAATCTTATCAATCAACTCGTCATCAACCCTGGTCAACTCCGATACTCTGTCGGACCAGCCCTGGGTTGTGCCGGAGGTGGAAGAATCTATGACTTCGAAGCCGCTTGGGATGCAATCTTAGCCAGCAGTGACTCTAGTTTCTTAAATGAAGAGTACTGCATCGTCAAGAGATTGTACAACTCTCGCAACAGCCAAAGCAACAACATCGCTGCCTACATCACCGCTCACTTACTTCCCCCGGTTGCTCAAGTGTTCCACCAATCAGCTGGATCAATCACAGACCTCCTGAGAGGCGTTGGCAACGGTAATGACGCGACCGGTTTAGTTGCTAATGCTCAAAGATATATTGCACAAGCAGCCAGCCAGGTTCACGTCTAA
Protein
MKPIFLVLLVATSAYAAPSVTINQYSDNEIPRDIDDGKASSVISRAWDYVDDTDKSIAILNVQEILKDMASQGDYASQASAVAQTAGIIAHLSAGIPGDACAAANVINSYTDGVRSGNFAGFRQSLGPFFGHVGQNLNLINQLVINPGQLRYSVGPALGCAGGGRIYDFEAAWDAILASSDSSFLNEEYCIVKRLYNSRNSQSNNIAAYITAHLLPPVAQVFHQSAGSITDLLRGVGNGNDATGLVANAQRYIAQAASQVHV

Summary

Description
It is likely that the major role of L-chain is to prevent the retention of H-chain in ER by forming the disulfide linkage.
Subunit
Silk fibroin elementary unit consists in a disulfide-linked heavy and light chain and a p25 glycoprotein in molar ratios of 6:6:1. This results in a complex of approximately 2.3 MDa.
Keywords
Acetylation   Complete proteome   Direct protein sequencing   Disulfide bond   Reference proteome   Secreted   Signal   Silk protein  
Feature
chain  Fibroin light chain
Uniprot
P21828   E1AZ89   Q7JYG3   Q9BLL9   Q8IT50   Q9BIF8   + More
Q9BLL5   A0A0N1PIW2   J9XSZ1   Q9BLL7   Q26427   A0A2A4JXS4   V5UTC1   D0EWD8   A0A194QD94   A0A3S2NFE9   A0A212EQ24   Q14UU5   D7PBW0   D7PBW3   D7PBW4   D7PBW1   D7PBW5   D7PBV9   D7PBW7   D7PBW6   D7PBW2   D7PBW8  
Pubmed
2585514    15591204    1347033    1972197    10366732    10986287    + More
11267905    26354079    7715595    20171223    22118469    16755355    20458474   
EMBL
X17291    CK545649    CK545775    M76430    HQ116534    ADM35104.1    + More
AY078388    AAL83649.1    AB001820    BAB39499.1    AF541967    AAN63945.1    AY026058    AAK13024.1    AB001824    BAB39503.1    KQ460144    KPJ17452.1    JX456150    AFS32690.1    AB001822    BAB39501.1    S77817    NWSH01000381    PCG76817.1    KF669645    AHB81536.1    GQ901975    ACX50392.1    KQ459337    KPJ01426.1    RSAL01000059    RVE49691.1    AGBW02013327    OWR43595.1    AB195977    BAE97693.1    GU180667    ADI52841.1    GU180670    ADI52844.1    GU180671    ADI52845.1    GU180668    ADI52842.1    GU180672    ADI52846.1    GU180666    ADI52840.1    GU180674    ADI52848.1    GU180673    ADI52847.1    GU180669    ADI52843.1    GU180675    ADI52849.1   
Proteomes
UP000005204    UP000053240    UP000218220    UP000053268    UP000283053    UP000007151    + More
PRIDE
P21828    Q9BLL9    Q8IT50    Q9BIF8    Q9BLL5    Q9BLL7    + More
Pfam
PF05849   L-fibroin
Interpro
IPR008660   L-fibroin       
ProteinModelPortal
P21828   E1AZ89   Q7JYG3   Q9BLL9   Q8IT50   Q9BIF8   + More
Q9BLL5   A0A0N1PIW2   J9XSZ1   Q9BLL7   Q26427   A0A2A4JXS4   V5UTC1   D0EWD8   A0A194QD94   A0A3S2NFE9   A0A212EQ24   Q14UU5   D7PBW0   D7PBW3   D7PBW4   D7PBW1   D7PBW5   D7PBV9   D7PBW7   D7PBW6   D7PBW2   D7PBW8  

Ontologies

KEGG
693047    no KO assigned | (RefSeq) Fib-l, FIBL, fibroin; silk fibroin light chain
GO
GO:0005576   GO:0016491   GO:0006468   GO:0003676   GO:0006182   GO:0051603   GO:0030145   GO:0006412   GO:0016876   GO:0043039  

Topology

Subcellular location
Secreted  
SignalP
Position:   1 - 16,         Likelihood:  0.999024
 
 
Length:
262
Number of predicted TMHs:
0
Exp number of AAs in TMHs:
1.24129
Exp number, first 60 AAs:
1.18211
Total prob of N-in:
0.04342
outside
1  -  262
 
 

Population Genetic Test Statistics

Pi
239.014506
Theta
159.823903
Tajima's D
1.658423
CLR
0.205713
CSRT
0.820058997050148
Interpretation
Uncertain
Peptides ×
Source Sequence Identity Evalue
25860555 QSSSSYQSQSYNK 97.92 7e-26
24093152 DKWPDCPTINEVR 97.92 7e-26
27102218 DIDDGKASSVISR 97.92 7e-26
20029844 YIAQAASQVHV 100.00 1e-15
20029844 NVQEILKDM*ASQGDYASQASAVAQ 100.00 7e-15
20029844 NVQEILKDMASQGDYASQ*ASAVAQ 100.00 7e-15
20029844 NVQEILKDM*ASQGDYASQ*ASAVAQ 100.00 7e-15
20029844 SVTINQY 100.00 6e-14
20029844 SVTINQYSDN*EIPRDIDDGKASSVI 100.00 6e-14
20029844 APSVTINQYSDNE*IPR 100.00 2e-13
20029844 YSVGPALGCAGGGR 100.00 2e-13
20029844 YSVGPALGCAGGGR 100.00 2e-13
20029844 NVQEILKDMASQGDYASQASAVAQTAGIIAHL 100.00 3e-12
20029844 SVTINQYSDNEIPRDIDDGKASSVI 96.67 3e-12
20029844 APSVTINQYSDNEIPRDIDDGKASSVISRAW 96.67 3e-12
20029844 ASQASAVAQ*TAGIIAHL 100.00 3e-12
20029844 SVTINQYSDNE*IPR 100.00 6e-12
20029844 S*VTINQYSDNEIPR 100.00 6e-12
20029844 APSVTINQYSDNEIPR 96.67 8e-12
20029844 YSVGPALGCAGGGR 96.55 1e-11
20029844 SVTINQYSDNEI*PRDIDDGKASSVISR 100.00 2e-11
20029844 S*VTINQYSDNEIPRDIDDGK 100.00 2e-11
20029844 NSQ*SNNIAAYITAH 100.00 4e-11
20029844 DYVDDTDKSIAILN 96.55 1e-10
20029844 YIAQAASQ*VHV 100.00 2e-10
20029844 AGIIAHLSAGIPGDACAAAN 96.43 2e-10
20029844 SAGIPGDACAAANVINSYTDGVRSGNF 96.43 2e-10
20029844 NVQEILKDM*ASQGDYASQASAVAQ 100.00 2e-10
20029844 P*SVTINQYSDNEIPR 96.43 3e-10
20029844 SVTINQYSDNEIPRDIDDGKASSVISR 96.43 3e-10
20029844 LSAGIPGDACAAANVINSYTDGVR 100.00 3e-10
20029844 DM*ASQGDYASQASAVAQTAGIIAH 100.00 6e-10
20029844 DM*ASQGDYASQASAVAQTAGIIAH 100.00 6e-10
20029844 DM*ASQGDYASQASAVAQTAGIIAH 100.00 6e-10
20029844 DM*ASQGDYASQ*ASAVAQTAGIIAH 100.00 6e-10
31253818 MIKTFVITTDSDGNESIVEEDVLmK 100.00 3e-09
30270257 ALPcVNc 100.00 3e-09
20029844 SVTINQYSDNEIPRDIDDGKASSVISR 100.00 3e-09
20029844 AWDYVDDTD*K 100.00 3e-09
20029844 DIDDGKASSVISR 100.00 3e-09
20029844 ITAHLLPPVAQVF 100.00 4e-09
20029844 AHLLPPVAQ*VF 100.00 4e-09
20029844 ITAHLLPPVAQ*VF 100.00 4e-09
20029844 HQ*SAGSITDLLRGV 100.00 4e-09
20029844 LLPPVAQ*VF 100.00 4e-09
20029844 NLINQLVI*NPGQLRY 100.00 5e-09
20029844 INQLVINPGQLRY 100.00 5e-09
20029844 NLINQ*LVINPGQLRY 100.00 5e-09
20029844 INQLVINPGQLRY 100.00 5e-09
20029844 Q*YSDNEIPRDIDDGKAS 100.00 7e-09
20029844 Q*YSDNEIPRDIDDGKAS 100.00 7e-09
20029844 SIAILNVQEI*LK 100.00 7e-09
20029844 SIAILNVQ*EILK 100.00 7e-09
20029844 YIAQAASQVHV 96.15 7e-09
20029844 LSAGIPGDACAAANVINSYTDGVR 96.00 3e-08
20029844 LSAGIPGDACAAANVINSYTDGVR 96.00 3e-08
20029844 NSQSNNIAAYITAH 100.00 3e-08
20029844 SDNE*IPRDIDDGKASSVISRAW 100.00 1e-07
20029844 AWDYVDDTDKSIAILNVQEILK 95.65 2e-07
20029844 HQSAGSITDLLRGVGNGNDATGL 95.83 2e-07
20029844 HQSAGSITDLLRGVGNGNDATGL 95.83 2e-07
20029844 HQSAGSITDLLRGVGNGNDATGL 95.83 2e-07
20029844 HQSAGSITDLLRGVGNGNDATGL 95.83 2e-07
20029844 LLPPVAQ*VF 95.83 2e-07
20029844 N*VQEILKDMASQGDY 96.00 2e-07
20029844 NVQEILKDM*ASQGDY 96.00 2e-07
20029844 NVQEILKDMASQGD*Y 96.00 2e-07
20029844 SDNEIPRDIDDGKASSVISRAW 95.65 3e-07
20029844 SIAILNVQE*ILK 96.00 3e-07
20029844 DMASQGDYASQASAVAQTAGIIAH 96.00 3e-07
20029844 DMASQGDYASQASAVAQTAGIIAH 96.00 3e-07
20029844 SVTINQYSDNE*IPR 100.00 4e-07
20029844 ITAHLLPPVAQVFHQSAGSITDLLR 100.00 4e-07
20029844 N*SQSNNIAAYITAH 100.00 4e-07
20029844 N*SQSNNIAAYITAH 100.00 4e-07
20029844 IYDFEAAWDAILASSDSSFLNEEYCIVK 95.45 5e-07
20029844 LSAGIPGDACAAAN*VINSYTDGVR 100.00 6e-07
20029844 Q*YSDNEIPRDIDDGKAS 100.00 8e-07
20029844 NVQEILKDMASQGDYASQASAVAQTAGIIAHL 100.00 2e-06
20029844 NEIPRDIDDGKASSVISRAW 100.00 3e-06
20029844 SVGPALGCAGGGRIYDFEAAW 100.00 1e-05
20029844 Q*YSDNEIPRDIDDGKAS 100.00 2e-05
20029844 Q*YSDNEIPRDIDDGKAS 100.00 2e-05
20029844 SAVAQTAGIIAHL 100.00 2e-05
20029844 ASQASAVAQTAGIIAHLSAGIPGDACAAAN 100.00 2e-05
20029844 SAGIPGDACAAANVINSY 100.00 2e-05
20029844 SAGIPGDACAAANVINSY 100.00 2e-05
20029844 SAGIPGDACAAANVIN*SYTDGVRSGNF 100.00 2e-05
20029844 AGFR*QSLGPF 100.00 4e-05
20029844 HQSAGSITDLLR*GVGNGNDATGL 100.00 5e-05
20029844 DLLRGVGN*GNDATGL 100.00 5e-05
20822545 DGLALTLSNDVHGNDGR 100.00 5e-05
20029844 IAQAASQVHV 100.00 8e-05
20029844 IAQAASQVHV 100.00 8e-05
20029844 SVTINQYSDNEIPR*DIDDGKASSVISRAW 100.00 8e-05
20029844 QYSDNEIPRDIDD 100.00 8e-05
20029844 SVTINQYSDNEIPR*DIDDGKASSVISRAW 100.00 8e-05
20029844 IAQAASQVHV 100.00 8e-05
20029844 SVTINQYSDNEIPRDIDDGKASSVISRAW 100.00 8e-05
20029844 VINPGQLRYSVGPALGC 100.00 9e-05
20029844 LLPPVAQVFHQSAGSITDLLR 100.00 1e-04
20029844 GVGN*GNDATGLVANAQR 100.00 1e-04
20029844 GVGN*GNDATGLVANAQR 100.00 1e-04
20029844 WDAILASSDSS*FLN 100.00 2e-04
20029844 WDAILASSDSSF 100.00 2e-04
20029844 RAW*DYVDDTDKSIAI 100.00 3e-04
20029844 SDNE*IPRDIDDGKAS 100.00 3e-04
20029844 SDNEIPRDIDDGKASSVISR 100.00 3e-04
20029844 QYSDNEIPRDIDDGKAS 100.00 3e-04
20029844 QNLNLINQ*L 100.00 4e-04
20029844 VI*NPGQLRYSVGPALGC 100.00 4e-04
20029844 S*VTINQYSDNEIPRDIDDGKASSVISR 100.00 5e-04
20029844 S*VTINQYSDNEIPRDIDDGKASSVISR 100.00 5e-04
20029844 QSLGPFFGHVGQNLNL 100.00 8e-04
20029844 DYVDDTDKSIAIL 100.00 9e-04
20029844 DYVDDTDKSIAILNVQEILKDM*ASQGDY 100.00 9e-04
20029844 NVQEILKDMASQGDYASQASAVAQTA 100.00 0.001
20029844 ANVIN*SYTDGVR 100.00 0.002
20029844 APSVTINQYSDNEIPRDIDDGKASSVISR 100.00 0.002
20029844 IAQAASQ*VHV 100.00 0.003
31253818 AWDYVDDTDK 100.00 0.003
30270257 AWDYVDDTDK 100.00 0.003
20029844 FGHVGQNLNLINQLVINPGQLR 100.00 0.003
20029844 LINQLVINPGQLR 100.00 0.003
20029844 LINQLVINPGQLR 100.00 0.003
20029844 HVGQNLNLINQLVI*NPGQLR 100.00 0.003
31250652 NTYGFNNIEASK 100.00 0.003
20029844 RQSLGPFFGHVGQ*NL 100.00 0.003
20029844 RQSLGPFFGHVGQNL 100.00 0.004
20029844 AGFRQSLGPFFGHVGQNL 100.00 0.004
20029844 AGFR*QSLGPF 100.00 0.004
20029844 AGFR*QSLGPF 100.00 0.004
20029844 NSRNSQSNNIAAY 100.00 0.004
20029844 NSRNSQSNNIAAY 100.00 0.004
20029844 LLPPVAQ 100.00 0.005
20029844 NVQEILKDMASQGDY 100.00 0.005
20029844 DYVDDTDKSIAILNVQEIL 100.00 0.007
20029844 NVQ*EILKDMASQGDY 100.00 0.007
20029844 NVQ*EILKDMASQGDY 100.00 0.007
20029844 SVTINQYSDNE*IPR 100.00 0.008
20029844 YSVGPALGCAGGGR 100.00 0.012
20029844 ASSDSS*FLNEEYCIVK 100.00 0.012
20029844 Q*YSDNEIPRDIDDGKAS 100.00 0.012
20029844 WDAILASSDSS*FLNEEYCIVK 100.00 0.012
20029844 RAW*DYVDDTDKSIAI 100.00 0.012
20029844 IYDFEAAWDAI*LASSDSSFLNEEYCIVK 100.00 0.012
20029844 SSDSS*FLNEEYCIVK 100.00 0.014
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