Gene

KWMTBOMO08444

Pre Gene Modal

BGIBMGA009403

Annotation

Glycosyltransferase-like_protein_LARGE2_[Papilio_xuthus]

Location

Bomo_Chr14(-):9295053-9313861      View in EpiBrowser

Full name

LARGE xylosyl- and glucuronyltransferase 1       + More
LARGE xylosyl- and glucuronyltransferase 2      

Alternative Name

Acetylglucosaminyltransferase-like 1A
Glycosyltransferase-like protein
Glycosyltransferase-like 1B

Location in the cell

Extracellular   Reliability : 1.11 PlasmaMembrane   Reliability : 1.412

CDS

ATGAAAGAAATTTCAGTAGAATATACTACCACATATCAAGTTCCTGTTATACCAGATTCTATAGGCACCTTGTGTGAAACAATTCATATAGCATTAGTTTGTATGGGTAAATGTACGAGACTGATCACTCCTATGTTTAAATCGTTGTTACACAACAGACAGAATCCGTTACATTTTCACATGGTCGTGGATAATACTGCAGAGCACACAGTCGCTGTGCTATTTGATACTTGGATGCTACCTGACGTAAAATACACATGCTACAACGCGCAGAATCGATTGTCCGAAGTCAGATGGATTCCCAACAGTCATTATTCCGGTGTATACGCTCTGGTCAAGCTGTTGTTCCCGGATATCCTGCCAGAGAACCTGGACCGGATCATTGTGTTGGACTCGGATTTGACCTTTCTCAGTGACGTAGCTGAGATCTGGAGGATGTTCAGGAACATGACCAACGAACAGTTTATAGGTTTGGTGGAAAACGAAAGCAATTGGTATTACGACACGGCGAAGCGATGGCCCGCGCTGGGCAGGGGCTTCAACACCGGCCTGATGCTGCTGGACTTGAAAAAGATCAGAACGAACATAGACTGGACTGATCTATGGCAGAACGCCATCAAGGAGAACATAGAGTCGCTGAAGCAGACGACCCTCGCCGACCAGGACATTATTAATGCCATCATAAAGAAACATCCTAATTACGTTTATAAAATTGGGTGTCAGTACAATGTGCAAATGTCGACGAAGACACTGTCCAAAAATTGTTATCGTGAAAATAAAAATAATGTTAAGGTCGTTCACTGGAATTCTCCAGCGAAGTACAATATAAAGATCAGAGACGCGCACTATTTCCGTAATCTATATCAGAGCTATGTTAATTTTGACGGGAATCTGTTGAGGGAGAAACTACATACGTGCAGTCAGAATGAAGCGCTGACTAATAAGATAAATTACTCCGATCTGTGTGTTAGTTTTCGTTCGGCTCAGAACATTAAGCTGCGTACTCACTTGTACTACATGGACTACAGCTACAATAATATCGACGACTTCGACGTCACCTTAGTTCTGCAGCTGTCCATGGACCGACTGCAGTTCCTGGAGAGACTTGTCAATCATTGGGAAGGTCCGTTAAGTGCAGCGATATATTTGTCGGACTGTGACGTCACGAAGCTGGAGAGCTTCACGCGGGACTGGTCGGACACGCTAAGCTCCCGGAAGAACATCGGATACCACTTAGTATTCAAACATGATTCGGTACATTACCCGGTGAATTATCTCCGTAACGTCGCGCTGGAGAATGTGAACACGCCCTACGTGTTCCTCATGGACGTCGACTTCGTTCCCATGGCGGGCCTGTACCAACATCTCAGGGACGCGATACGACTCATAAACCCTTATCCACAGAAAAAGTGTCTGGTGGTGCCCGCGTTCGAGACGCAGCGGTACCGCGCGTCCGTGCCGCGCGACAAGGCGGCGCTGCTGGCGCGGCTGGCGGCGCGGCACGGCGCCGGGGACGTCGCGCCCTTCCGCACCCGGGAGTGGCCGCGCGGACACCGGGCCACCAACTACACGAGGTGGATGACCGCCACTGCTCCTTATGAGGTGGAGTGGGAGTCCGACTACGAGCCCTACCTGGTGGTGCACCGGTCGGTACCTAAATACGACACCAGATTCTCGGGCTTCGGCTGGAACAAGGTGTCGCACAGCGTGGAGCTGCGCGCGACCGGGCACCGCGCGCTCGTGCTGCCGGCCGCGTTCCTCGTGCACACGCCGCACGCGCCCTCGCCCGACATCACCGCCTTCCGCGCCGACCCGCACTACCGCGTTTGTCTCGCGTTGTTGAAACAAGAATTCATGGACGACCTGAGCAGGAAGTATAACATGACCCTGGGAGGACCTTCGAAATTGGACAGTGTGTACCTCGGACAGGCCAAGAGCTTGCTGCTGAACCAGGCTAAGGAGTTGCCTTAG

Protein

MKEISVEYTTTYQVPVIPDSIGTLCETIHIALVCMGKCTRLITPMFKSLLHNRQNPLHFHMVVDNTAEHTVAVLFDTWMLPDVKYTCYNAQNRLSEVRWIPNSHYSGVYALVKLLFPDILPENLDRIIVLDSDLTFLSDVAEIWRMFRNMTNEQFIGLVENESNWYYDTAKRWPALGRGFNTGLMLLDLKKIRTNIDWTDLWQNAIKENIESLKQTTLADQDIINAIIKKHPNYVYKIGCQYNVQMSTKTLSKNCYRENKNNVKVVHWNSPAKYNIKIRDAHYFRNLYQSYVNFDGNLLREKLHTCSQNEALTNKINYSDLCVSFRSAQNIKLRTHLYYMDYSYNNIDDFDVTLVLQLSMDRLQFLERLVNHWEGPLSAAIYLSDCDVTKLESFTRDWSDTLSSRKNIGYHLVFKHDSVHYPVNYLRNVALENVNTPYVFLMDVDFVPMAGLYQHLRDAIRLINPYPQKKCLVVPAFETQRYRASVPRDKAALLARLAARHGAGDVAPFRTREWPRGHRATNYTRWMTATAPYEVEWESDYEPYLVVHRSVPKYDTRFSGFGWNKVSHSVELRATGHRALVLPAAFLVHTPHAPSPDITAFRADPHYRVCLALLKQEFMDDLSRKYNMTLGGPSKLDSVYLGQAKSLLLNQAKELP

Description

Bifunctional glycosyltransferase with both xylosyltransferase and beta-1,3-glucuronyltransferase activities involved in the biosynthesis of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4-(phosphate-6-)mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1) (PubMed:23125099, PubMed:23135544). Phosphorylated O-mannosyl trisaccharid is required for binding laminin G-like domain-containing extracellular proteins with high affinity and plays a key role in skeletal muscle function and regeneration (PubMed:15184894, PubMed:24132234). LARGE elongates the glucuronyl-beta-1,4-xylose-beta disaccharide primer structure initiated by B3GNT1/B4GAT1 by adding repeating units [-3-Xylose-alpha-1,3-GlcA-beta-1-] to produce a heteropolysaccharide (By similarity).
Bifunctional glycosyltransferase with both xylosyltransferase and beta-1,3-glucuronyltransferase activities involved in the biosynthesis of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4-(phosphate-6-)mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1). Phosphorylated O-mannosyl trisaccharid is required for binding laminin G-like domain-containing extracellular proteins with high affinity. Elongates the glucuronyl-beta-1,4-xylose-beta disaccharide primer structure by adding repeating units [-3-Xylose-alpha-1,3-GlcA-beta-1-] to produce a heteropolysaccharide. Has a higher activity toward alpha-dystroglycan than LARGE.
Bifunctional glycosyltransferase with both xylosyltransferase and beta-1,3-glucuronyltransferase activities involved in the biosynthesis of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4-(phosphate-6-)mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1) (PubMed:22223806). Phosphorylated O-mannosyl trisaccharid is required for binding laminin G-like domain-containing extracellular proteins with high affinity and plays a key role in skeletal muscle function and regeneration. LARGE elongates the glucuronyl-beta-1,4-xylose-beta disaccharide primer structure initiated by B3GNT1/B4GAT1 by adding repeating units [-3-Xylose-alpha-1,3-GlcA-beta-1-] to produce a heteropolysaccharide (PubMed:25279699).

Cofactor

Mn(2+)

Subunit

Interacts with DAG1 (via the N-terminal domain of alpha-DAG1); the interaction increases binding of DAG1 to laminin. Interacts with B3GNT1/B4GAT1.
Interacts with DAG1 (via the N-terminal domain of alpha-DAG1); the interaction increases binding of DAG1 to laminin (By similarity). Interacts with B3GNT1/B4GAT1 (PubMed:19587235).

Similarity

In the C-terminal section; belongs to the glycosyltransferase 49 family.
In the N-terminal section; belongs to the glycosyltransferase 8 family.

Keywords

Coiled coil   Complete proteome   Glycoprotein   Glycosyltransferase   Golgi apparatus   Manganese   Membrane   Metal-binding   Multifunctional enzyme   Reference proteome   Signal-anchor   Transferase   Transmembrane   Transmembrane helix   Alternative splicing   Congenital muscular dystrophy   Disease mutation   Dystroglycanopathy   Lissencephaly   Polymorphism  

Feature

chain  LARGE xylosyl- and glucuronyltransferase 1
splice variant  In isoform 2.
sequence variant  In dbSNP:rs470035.

Uniprot

A0A437BH37   A0A0N1PHL8   A0A2A4K034   A0A212FC44   A0A2H1VJT8   A0A2H1V8R8   + More
A0A0N0PE39   A0A2L2Y9Z4   T1JEW5   E0VGD3   R7TJK9   A0A1B6CYE9   A0A1D2N9G8   A0A0A9YJR9   A0A3S3QIJ1   A0A0G2K618   A0A023F5B6   K7FUI6   Q059X9   Q9Z1M7   F7BXD5   A0A147BEK3   H0WH59   B3DFN1   A0A1S3FWF7   A0A250Y4X4   A0A1U7U2V0   A0A2K6EKN8   A0A3S1BCW5   Q66PG1   G1SG12   A0A2K5YPC0   A0A2Y9GVR2   A0A2J7PG24   A0A1U8DIB7   A0A3Q0GZD9   A0A1A7YWM9   G3TKC6   A0A2J7PG28   V5IHD6   I3MWQ4   G1MCE4   F1P723   A0A2K5C7A4   M3WNA3   A0A3Q7TN28   M3YLT6   A0A2K5PFQ5   G7N3P5   A0A2J8UVN3   A0A2K6TC39   A0A3P8XNA6   H3AH90   A0A1S3KT15   A0A0D9R7A4   A0A2K6PR53   A0A2Y9KZW9   A0A3Q7V2H6   G7PFA2   A0A2K5P157   A0A2K6AN31   A0A2K6K897   A0A096NJQ6   F6QJZ2   H2QZS2   A0A1Z5KUC0   X5DR28   O95461   A0A151NHX1   A0A452ISM3   A0A3Q3JPB6   H0VRT1   G1P039   L5LIU7   A0A402EM90   F6W6X6   A0A2U3X4A5   A0A067QRU2   A0A1S3PRW3   K9INM3   T1HYF4   A0A060WGJ6   A0A1A8MAF7   A0A1A7XMX1   A0A3B4CR51   A0A3B3XA23   A0A452ERG5   A0A3Q2I5B9   E1BDI0   A0A1A8BHC4   A0A091IPA3   A0A1A8KBF5   A0A1A8B0W5   A0A1A8S7R4   A0A2D0QX56   A0A226E869   M3ZES1   A0A1V4K7K2   A0A0F7Z8J2   A0A3Q2PUR7  

EC Number

2.4.-.-

Pubmed

26354079    22118469    26561354    20566863    23254933    27289101    + More
25401762    26823975    15057822    25474469    17381049    12040188    15489334    9892679    12693553    11381262    15210115    15958417    15184894    23125099    23135544    24132234    25138275    25243066    29652888    28087693    23594743    21993624    25765539    20010809    16341006    17975172    22002653    25069045    9215903    25362486    17431167    25319552    15164055    16136131    28528879    11181995    24722188    9628581    15461802    10591208    15752776    15661757    19587235    21987822    22223806    25279699    25279697    12966029    19067344    19299310    24709677    22293439    28562605    19892987    24845553    24755649    19393038    23542700   

EMBL

RSAL01000059    RVE49710.1    KQ458751    KPJ05299.1    NWSH01000337    PCG77288.1    + More
AGBW02009237    OWR51287.1    ODYU01002686    SOQ40514.1    ODYU01001253    SOQ37207.1    KQ459986    KPJ18829.1    IAAA01016561    IAAA01016562    LAA04992.1    JH432130    DS235137    EEB12439.1    AMQN01013876    KB310564    ELT91285.1    GEDC01018877    JAS18421.1    LJIJ01000134    ODN01913.1    GBHO01011738    GDHC01020207    JAG31866.1    JAP98421.1    NCKU01002576    RWS09293.1    AABR07042861    AABR07042862    AABR07042863    AABR07042864    AABR07042865    AABR07042866    GBBI01002408    JAC16304.1    AGCU01195138    AGCU01195139    AGCU01195140    AGCU01195141    AGCU01195142    AGCU01195143    AGCU01195144    AGCU01195145    AGCU01195146    AGCU01195147    BC125487    BC125489    CH466525    AAI25490.1    EDL10819.1    AJ006278    AK122328    BC061506    BC100399    GAMT01006968    GAMS01004399    GAMR01008255    GAMQ01004062    GAMQ01004061    GAMP01003926    GAMP01003925    GAMP01003924    JAB04893.1    JAB18737.1    JAB25677.1    JAB37789.1    JAB48829.1    GEGO01006659    JAR88745.1    AAQR03016478    AAQR03016479    AAQR03016480    AAQR03016481    AAQR03016482    BC162103    AAI62103.1    GFFV01001295    JAV38650.1    RQTK01000359    RUS81043.1    AY662339    BX908385    BX936304    AAGW02060986    AAGW02060987    AAGW02060988    AAGW02060989    AAGW02060990    AAGW02060991    AAGW02060992    NEVH01025635    PNF15282.1    HADX01012128    SBP34360.1    PNF15281.1    GANP01005122    JAB79346.1    AGTP01024453    AGTP01024454    AGTP01024455    AGTP01024456    AGTP01024457    AGTP01024458    AGTP01024459    AGTP01024460    AGTP01024461    AGTP01024462    ACTA01075690    ACTA01083690    ACTA01091690    ACTA01099690    ACTA01107690    ACTA01115690    ACTA01123690    ACTA01131690    ACTA01139690    ACTA01147689    AAEX03007366    AANG04003655    AEYP01012497    AEYP01012498    AEYP01012499    AEYP01012500    AEYP01012501    AEYP01012502    AEYP01012503    AEYP01012504    AEYP01012505    AEYP01012506    CM001262    EHH20177.1    ABGA01343582    ABGA01343583    ABGA01343584    ABGA01343585    ABGA01343586    ABGA01343587    ABGA01343588    ABGA01343589    ABGA01343590    ABGA01343591    NDHI03003442    PNJ49333.1    PNJ49334.1    AFYH01014743    AFYH01014744    AFYH01014745    AFYH01014746    AFYH01014747    AFYH01014748    AFYH01014749    AFYH01014750    AFYH01014751    AFYH01014752    AQIB01136734    AQIB01136735    AQIB01136736    AQIB01136737    AQIB01136738    AQIB01136739    AQIB01136740    AQIB01136741    AQIA01007180    AQIA01007181    AQIA01007182    AQIA01007183    AQIA01007184    AQIA01007185    AQIA01007186    AQIA01007187    AQIA01007188    CM001285    EHH65786.1    AHZZ02001146    AHZZ02001147    AHZZ02001148    AHZZ02001149    AHZZ02001150    AHZZ02001151    AHZZ02001152    AHZZ02001153    JSUE03005380    JSUE03005381    JSUE03005382    JSUE03005383    JSUE03005384    JSUE03005385    JSUE03005386    JSUE03005387    JSUE03005388    JSUE03005389    JU337091    AFE80844.1    AACZ04068072    GABC01005918    GABC01005917    GABF01000316    GABF01000315    GABD01008726    GABD01008725    GABE01009849    GABE01009848    NBAG03000026    JAA05420.1    JAA21829.1    JAA24374.1    JAA34890.1    PNI98083.1    PNI98084.1    GFJQ02008355    JAV98614.1    KJ534877    CH471095    AHW56517.1    EAW60041.1    AJ007583    AB011181    CR456510    AL008630    AL008715    AL096754    Z68287    Z69042    Z69943    Z70288    Z82173    BC117425    BC126404    AKHW03002956    KYO36407.1    AAKN02054133    AAPE02024977    AAPE02024978    AAPE02024979    AAPE02024980    AAPE02024981    AAPE02024982    AAPE02024983    AAPE02024984    AAPE02024985    AAPE02024986    KB111329    ELK25930.1    BDOT01000023    GCF45258.1    KK853033    KDR12249.1    GABZ01004573    JAA48952.1    ACPB03007941    FR904535    CDQ66232.1    HAEF01012155    SBR53314.1    HADW01018032    SBP19432.1    LWLT01000005    HADZ01002352    SBP66293.1    KK500774    KFP10182.1    HAEE01009582    SBR29632.1    HADY01022083    SBP60568.1    HAEI01011532    SBS13594.1    LNIX01000006    OXA53187.1    LSYS01004200    OPJ80414.1    GBEX01001759    JAI12801.1   

Proteomes

UP000283053    UP000053268    UP000218220    UP000007151    UP000053240    UP000009046    + More
UP000014760    UP000094527    UP000285301    UP000002494    UP000007267    UP000000589    UP000008225    UP000005225    UP000081671    UP000189704    UP000233160    UP000271974    UP000000437    UP000001811    UP000233140    UP000248481    UP000235965    UP000189705    UP000007646    UP000005215    UP000008912    UP000002254    UP000233020    UP000011712    UP000286640    UP000000715    UP000233040    UP000001595    UP000233220    UP000265140    UP000008672    UP000087266    UP000029965    UP000233200    UP000248482    UP000286642    UP000009130    UP000233100    UP000233060    UP000233120    UP000233180    UP000028761    UP000006718    UP000002277    UP000005640    UP000050525    UP000291020    UP000261600    UP000005447    UP000001074    UP000288954    UP000002281    UP000245340    UP000027135    UP000015103    UP000193380    UP000261440    UP000261480    UP000291000    UP000009136    UP000053119    UP000221080    UP000198287    UP000002852    UP000190648    UP000265000   

PRIDE

Q9Z1M7    Q66PG1    O95461   

Pfam

PF01501   Glyco_transf_8

Interpro

IPR029044   Nucleotide-diphossugar_trans        + More
IPR002495   Glyco_trans_8       

SUPFAM

SSF53448   SSF53448       

Gene 3D

3.90.550.10    

ProteinModelPortal

A0A437BH37   A0A0N1PHL8   A0A2A4K034   A0A212FC44   A0A2H1VJT8   A0A2H1V8R8   + More
A0A0N0PE39   A0A2L2Y9Z4   T1JEW5   E0VGD3   R7TJK9   A0A1B6CYE9   A0A1D2N9G8   A0A0A9YJR9   A0A3S3QIJ1   A0A0G2K618   A0A023F5B6   K7FUI6   Q059X9   Q9Z1M7   F7BXD5   A0A147BEK3   H0WH59   B3DFN1   A0A1S3FWF7   A0A250Y4X4   A0A1U7U2V0   A0A2K6EKN8   A0A3S1BCW5   Q66PG1   G1SG12   A0A2K5YPC0   A0A2Y9GVR2   A0A2J7PG24   A0A1U8DIB7   A0A3Q0GZD9   A0A1A7YWM9   G3TKC6   A0A2J7PG28   V5IHD6   I3MWQ4   G1MCE4   F1P723   A0A2K5C7A4   M3WNA3   A0A3Q7TN28   M3YLT6   A0A2K5PFQ5   G7N3P5   A0A2J8UVN3   A0A2K6TC39   A0A3P8XNA6   H3AH90   A0A1S3KT15   A0A0D9R7A4   A0A2K6PR53   A0A2Y9KZW9   A0A3Q7V2H6   G7PFA2   A0A2K5P157   A0A2K6AN31   A0A2K6K897   A0A096NJQ6   F6QJZ2   H2QZS2   A0A1Z5KUC0   X5DR28   O95461   A0A151NHX1   A0A452ISM3   A0A3Q3JPB6   H0VRT1   G1P039   L5LIU7   A0A402EM90   F6W6X6   A0A2U3X4A5   A0A067QRU2   A0A1S3PRW3   K9INM3   T1HYF4   A0A060WGJ6   A0A1A8MAF7   A0A1A7XMX1   A0A3B4CR51   A0A3B3XA23   A0A452ERG5   A0A3Q2I5B9   E1BDI0   A0A1A8BHC4   A0A091IPA3   A0A1A8KBF5   A0A1A8B0W5   A0A1A8S7R4   A0A2D0QX56   A0A226E869   M3ZES1   A0A1V4K7K2   A0A0F7Z8J2   A0A3Q2PUR7  

PDB

4WNH     E-value=2.62062e-07,     Score=133

KEGG

101744219    K09668 glycosyltransferase-like protein LARGE [EC:2.4.2.- 2.4.1.-] | (RefSeq) glycosyltransferase-li

PATHWAY

00515    Mannose type O-glycan biosynthesis - Bombyx mori (domestic silkworm)
01100    Metabolic pathways - Bombyx mori (domestic silkworm)

GO

GO:0016757   GO:0016021   GO:0016740   GO:0042285   GO:0030145   GO:0005794   GO:0035269   GO:0015020   GO:0043403   GO:0060538   GO:0046716   GO:0009101   GO:0043231   GO:0000139   GO:0006486   GO:0046872   GO:0008375   GO:0006044   GO:0030173   GO:0035252   GO:0006493   GO:0006688   GO:0005524   GO:0006468   GO:0003676   GO:0006182   GO:0051603   GO:0006412   GO:0016876   GO:0043039  

Subcellular location

Golgi apparatus membrane  

Length:

656

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.350820000000001

Exp number, first 60 AAs:

0.32967

Total prob of N-in:

0.01608

outside

1  -  656

 

 

Pi

227.024865

Theta

188.91874

Tajima's D

0.940744

CLR

0.157828

CSRT

0.644517774111294

Interpretation

Uncertain