Gene

KWMTBOMO08246

Pre Gene Modal

BGIBMGA009517

Annotation

PREDICTED:_DNA_topoisomerase_3-alpha_[Amyelois_transitella]

Location

Bomo_Chr14(+):2595474-2609518      View in EpiBrowser

Full name

DNA topoisomerase      

Location in the cell

Mitochondrial   Reliability : 1.016

CDS

ATGAAGACAGCTCTTATGGTGGCGGAAAAGCCATCGTTGGCCCAAAATCTAGCCAACATACTTAGCAATGGCAAATGCACTACAACTAAAGGTATGAATACAGCATGTTCTATCCACGAATGGACCGGGATGTTCAAAAACGATTCGGTTCGTTTCAAAATGACTTCTGTCTGTGGTCACGTTATGACTTTGGATTTCACCGGCAAATACAACAATTGGGATAAGGTAGATCCTGTTGAGTTGTTCATCTGTCCGACGGAAAAGAAAGAATCGATGCCACGACTCCGGATACCGGCTTTTCTAGCCCAGGAGTCTAGGGGATGTGATTATCTAATACTGTGGTTGGATTGTGATAAAGAAGGGGAGAATATTTGCTTCGAGGTAATGAGTTGCGTGCAACCTTATATGAAAGGAGATGTTCTTTCGCCCCTTGTTACCTACCGGGCCCACTTTTCGGCTATCACAGAAAAGGACATCAAAGCAGCCATGCTGAATCTCGGAAGACCCAATGAAAATCAATCTAGAAGTGTTGATGCTCGTCAAGAACTCGATTTGAGAATTGGATGTGCTTTTACTAGATTTCAAACTAAATATTTTCAAGGAAGGTATGGAGATTTGGATGCTTCACTCATATCGTACGGTCCATGCCAGACTCCGACCCTGGGGTTTTGCGTGCAGCGTCACGACGAGATCCAAACCTTCAAGCCAGAAACTTACTGGGTGTTGCGTGTAACGGCTAGTACGTCTGAAGGAAGGGAATTGCCGCTTGAGTGGAAACGAGTCAGATCTTTTGAGAAGGACATTGCTAATATGTTCCTCTCAGGAATTAAGGAGTGTAAAGAAGCTACTGTGATCAATATCCAAGCCAAAGAGAAAGTGAAGGCGCGTCCAACCGCCCTGAACACAGTGGAACTGATGAGGGTCGCCAGCGCCGGCCTGGGGATGGGACCGCACCATGCAATGGCGGTTGCGGAGAGACTGTACACTCAAGGATATATCTCCTACCCTCGTACCGAAACCACAAGCTACTCAGAGAATTTTGATCTCATGGGAACCCTCCGTCAACAGCAGAGCTCAAGTAAGTGGGGCGCAGAGGTACGGGCACTGATCTCCAACGGCATCAACAAGCCGAAGAAGGGCCACGACGCGGGCGACCACCCCCCCATCACCCCCTTAAGGGCTGCTTCGGAGTCGGAGCTGGAGGGCGACATGTGGCGCATCTACGACTACGTGACGCGGCACTTCATAGCGACGCTGTCGCGCGACTGCAAGTTCCTCTCCACCACCATCACCTTCCGCATCGCCGCCGAGACCTTCCACTACACCGGCAACGCGCTGCTCGACGCCGGCTACACCGAGCTCATGCACTGGCAGGCGTTCGGCAAGGACGAGTTCGTGCCGACGCTGCACGTGGACGACGTGCTGCGCGCGCACGACCACCGCCTCGTGGAGTGCCAGACCGCGCCGCCGGACTACCTCACCGAGTCCGAGGTAATCACCCTGATGGAGAAGCACGGCATCGGCACGGACGCGTCCATCCCGGTGCACATCAACAACATCTGCCAGAGGAACTACGTGACGGTGGGCAGCGGCCGCCGCCTCGTGCCCACCAACCTCGGCATCGTCCTCGTGCACGGATACCAGAAGATCGACCCCGAGCTGGTGCTGCCCACGATGCGCTCGGCCGTGGAGGAGCAGCTCAACCTGATCGCCGTGGGGCGCGCGGACTTCCACGCCGTGCTGTCGCACACCACCGAGATATTCCGTAGGAAGTTCCAGTACTTCGTGAGGTCCATCGAGGGCATGGACCAGCTGTTCGAGGTCAGCTTCTCGTCGCTGAAGGCCAGCGGGAAGGCGCTGTCCAGGTGCGGCAAGTGCAGGCGCTACATGAGATACATACAGGCGAAGCCTGCCCGTCTCCACTGCTCGCACTGCGACGACACGTACTGCCTGCCGCAGAACGGCACCGTCCGCATCTACCGCGAGCTCAAGTGTCCGCTCGACGACTTCGAGCTGCTCTCCTGGTCCTCCGGCAGCAAGGGGAAGAGCTTCCCCCTCTGCCCCTACTGCTACAACCATCCGCCCTTCAGGGACATGAAGAAGGGTTTCGGTTGCAACTCGTGCACGCACCCGACGTGTCCGTACGGCGTCAACTGGACCGGAGTCTCCGGCTGCGTCGAGTGCGAGGCCGGCGTGCTCGTGCTCGACCCCGCCGCGCCGCGCTGGAAGCTCGCCTGCAACCGGTGCGACGTGATCATAAACATATTCGAGGACGCGGCGCGCGTGTCGGTGTGCGAGCAGGCGTGCGCGGCGTGCGGCGCGCAGCTGGTGGCGGTGGAGTACCGGCCCGAGCGCACCCGCCTGCCTGCCGCGCTCACCGAGATGACGGCCTGCCTCTACTGCGAGCCCATCTTCAGCGCCCTGGTTGAGAAGCACCGTGCGGTGACGTCACGCGCGGGCGGCGGGGCGGCGCGGGGCGCGGGGCGGGGCGGGCGGCCGCGGGTGCACAAGCACCGGGCCAAGCAGCCCAAGGACAAGATGGCGCTCCTGGCCGCGTACTTTGTATGA

Protein

MKTALMVAEKPSLAQNLANILSNGKCTTTKGMNTACSIHEWTGMFKNDSVRFKMTSVCGHVMTLDFTGKYNNWDKVDPVELFICPTEKKESMPRLRIPAFLAQESRGCDYLILWLDCDKEGENICFEVMSCVQPYMKGDVLSPLVTYRAHFSAITEKDIKAAMLNLGRPNENQSRSVDARQELDLRIGCAFTRFQTKYFQGRYGDLDASLISYGPCQTPTLGFCVQRHDEIQTFKPETYWVLRVTASTSEGRELPLEWKRVRSFEKDIANMFLSGIKECKEATVINIQAKEKVKARPTALNTVELMRVASAGLGMGPHHAMAVAERLYTQGYISYPRTETTSYSENFDLMGTLRQQQSSSKWGAEVRALISNGINKPKKGHDAGDHPPITPLRAASESELEGDMWRIYDYVTRHFIATLSRDCKFLSTTITFRIAAETFHYTGNALLDAGYTELMHWQAFGKDEFVPTLHVDDVLRAHDHRLVECQTAPPDYLTESEVITLMEKHGIGTDASIPVHINNICQRNYVTVGSGRRLVPTNLGIVLVHGYQKIDPELVLPTMRSAVEEQLNLIAVGRADFHAVLSHTTEIFRRKFQYFVRSIEGMDQLFEVSFSSLKASGKALSRCGKCRRYMRYIQAKPARLHCSHCDDTYCLPQNGTVRIYRELKCPLDDFELLSWSSGSKGKSFPLCPYCYNHPPFRDMKKGFGCNSCTHPTCPYGVNWTGVSGCVECEAGVLVLDPAAPRWKLACNRCDVIINIFEDAARVSVCEQACAACGAQLVAVEYRPERTRLPAALTEMTACLYCEPIFSALVEKHRAVTSRAGGGAARGAGRGGRPRVHKHRAKQPKDKMALLAAYFV

Description

Introduces a single-strand break via transesterification at a target site in duplex DNA. Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand.

Catalytic Activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.

Similarity

Belongs to the type IA topoisomerase family.

Uniprot

A0A3S2PIK3   A0A2A4JJL8   A0A212ESX6   A0A0N1PEI4   A0A2H1W0W7   S4PFB5   + More
A0A2W1BR09   A0A1B6LHD3   A0A2J7QEM5   K7J2S1   E2A762   A0A232EJL0   A0A0C9RG42   A0A151IN68   F4WE22   A0A026WQ46   A0A151IVT2   A0A088A352   E2BPS8   A0A151WNX3   A0A310SFP4   E0VDP5   A0A2A3EA46   A0A224XBE9   A0A1Y1MA13   A0A023F3Y2   A0A0M8ZUZ4   D2A047   A0A151K0G5   A0A195BAH3   A0A0L7QQI5   A0A154PEF0   A0A0A9Y6R2   A0A1B6EGG0   A0A1B6I6P9   A0A2P8ZM23   T1JEW7   A0A0P4W2G4   A0A1B6HKS4   A0A2R7W2B3   A0A1Q3FEG8   A0A1B0CLS7   A0A3Q3E9A5   A0A182GJK0   B0WQT7   A0A3P9PTQ9   A0A1I8QFA3   M3ZQ84   A0A3Q1IA97   A0A3B5LUS0   A0A182FR54   Q16PL8   A0A3B3U7A3   A0A1S3JW12   A0A087XWE3   A0A084VTN2   A0A3P8VKB6   A0A1W4Y7L5   A0A1A8NXZ4   C3Z1Q7   A0A3B5AEE8   A0A3B4CVT6   A0A2Y9E4X8   A0A3Q3MQX9   A0A1A8DV13   A0A1A8A0H9   A0A1A8ETH7   I3NC26   A0A182QKB4   A0A3B4U471   H9G5D6   A0A1A8K0W6   Q7ZWP8   T1DLK2   A0A452IL90   A0A2I0M851   A0A2D0PN12   A0A3Q2ZX36   W5K0Z9   R7VUT8   A0A1U7RK97   A0A1A7XLQ2   A0A146P593   A0A1V4JRS6   A0A3B4XNN5   A0A2I0UA86   Q5ZK71   A0A0K8RW07   A0A0P4VX55   A0A3Q2Y361   A0A182J079   A0A0L0CFQ1   H2MSA1   F1QNX4   G3TL95   A0A3B3SRF3   A0A3P9KYT8   A0A2K5E7V3   A0A224YXE5   A0A2I4CSL9  

EC Number

5.6.2.2

Pubmed

22118469    26354079    23622113    28756777    20075255    20798317    + More
28648823    21719571    24508170    30249741    20566863    28004739    25474469    18362917    19820115    25401762    26823975    29403074    26483478    23542700    17510324    24438588    24487278    18563158    21881562    23758969    28562605    23371554    25329095    15592404    15642098    16880537    25727380    26108605    17554307    23594743    29240929    28797301   

EMBL

RSAL01000021    RVE52410.1    NWSH01001168    PCG72285.1    AGBW02012702    OWR44579.1    + More
KQ458751    KPJ05315.1    ODYU01005536    SOQ46482.1    GAIX01004087    JAA88473.1    KZ149927    PZC77529.1    GEBQ01016983    JAT22994.1    NEVH01015307    PNF27039.1    GL437267    EFN70718.1    NNAY01003983    OXU18534.1    GBYB01012107    JAG81874.1    KQ976959    KYN06794.1    GL888102    EGI67460.1    KK107131    QOIP01000012    EZA58157.1    RLU16073.1    KQ980884    KYN11909.1    GL449658    EFN82279.1    KQ982905    KYQ49491.1    KQ765605    OAD53846.1    DS235083    EEB11501.1    KZ288322    PBC28162.1    GFTR01008162    JAW08264.1    GEZM01036476    JAV82662.1    GBBI01002576    JAC16136.1    KQ435878    KOX70069.1    KQ971338    EFA01746.1    KQ981296    KYN43100.1    KQ976532    KYM81543.1    KQ414786    KOC60880.1    KQ434889    KZC10246.1    GBHO01014852    GBRD01013717    GDHC01009898    JAG28752.1    JAG52109.1    JAQ08731.1    GEDC01000317    JAS36981.1    GECU01025136    JAS82570.1    PYGN01000019    PSN57539.1    JH432130    GDRN01090158    JAI60517.1    GECU01032430    JAS75276.1    KK854263    PTY13894.1    GFDL01009143    JAV25902.1    AJWK01017571    AJWK01017572    JXUM01001688    KQ560126    KXJ84335.1    DS232045    EDS33017.1    CH477778    EAT36302.1    AYCK01007111    ATLV01016368    KE525079    KFB41326.1    HAEH01004425    SBR73893.1    GG666573    EEN53424.1    HADZ01010219    HAEA01009302    SBQ37782.1    HADY01009230    HAEJ01013342    SBP47715.1    HAEB01003264    SBQ49791.1    AGTP01078054    AXCN02001599    AAWZ02023260    HAED01017964    HAEE01006015    SBR26035.1    BC046848    AAH46848.1    GAAZ01000703    JAA97240.1    AKCR02000030    PKK25855.1    KB376592    EMC88926.1    HADW01017355    SBP18755.1    GCES01147387    GCES01146809    JAQ38935.1    LSYS01006629    OPJ74861.1    KZ505949    PKU42903.1    AADN05000505    AB215105    AJ720213    BAD99111.1    CAG31872.1    GBKC01001007    JAG45063.1    GDRN01090157    JAI60518.1    JRES01001034    JRES01000455    KNC26049.1    KNC31032.1    BX005237    CR933528    GFPF01007304    MAA18450.1   

Proteomes

UP000283053    UP000218220    UP000007151    UP000053268    UP000235965    UP000002358    + More
UP000000311    UP000215335    UP000078542    UP000007755    UP000053097    UP000279307    UP000078492    UP000005203    UP000008237    UP000075809    UP000009046    UP000242457    UP000053105    UP000007266    UP000078541    UP000078540    UP000053825    UP000076502    UP000245037    UP000092461    UP000261660    UP000069940    UP000249989    UP000002320    UP000242638    UP000095300    UP000002852    UP000265040    UP000261380    UP000069272    UP000008820    UP000261500    UP000085678    UP000028760    UP000030765    UP000265120    UP000192224    UP000001554    UP000261400    UP000261440    UP000248480    UP000261640    UP000005215    UP000075886    UP000261420    UP000001646    UP000291020    UP000053872    UP000221080    UP000264800    UP000018467    UP000189705    UP000265000    UP000190648    UP000261360    UP000000539    UP000264820    UP000075880    UP000037069    UP000001038    UP000000437    UP000007646    UP000261540    UP000265180    UP000233020    UP000192220   

Pfam

PF01751   Toprim        + More
PF01131   Topoisom_bac

Interpro

IPR003601   Topo_IA_2        + More
IPR023405   Topo_IA_core_domain       
IPR013826   Topo_IA_cen_sub3       
IPR013497   Topo_IA_cen       
IPR006171   TOPRIM_domain       
IPR023406   Topo_IA_AS       
IPR034144   TOPRIM_TopoIII       
IPR000380   Topo_IA       
IPR013825   Topo_IA_cen_sub2       
IPR013824   Topo_IA_cen_sub1       
IPR003602   Topo_IA_DNA-bd_dom       

SUPFAM

SSF56712   SSF56712       

Gene 3D

1.10.290.10     1.10.460.10     2.70.20.10    

CDD

cd00186  TOP1Ac
cd03362  TOPRIM_TopoIA_TopoIII

ProteinModelPortal

A0A3S2PIK3   A0A2A4JJL8   A0A212ESX6   A0A0N1PEI4   A0A2H1W0W7   S4PFB5   + More
A0A2W1BR09   A0A1B6LHD3   A0A2J7QEM5   K7J2S1   E2A762   A0A232EJL0   A0A0C9RG42   A0A151IN68   F4WE22   A0A026WQ46   A0A151IVT2   A0A088A352   E2BPS8   A0A151WNX3   A0A310SFP4   E0VDP5   A0A2A3EA46   A0A224XBE9   A0A1Y1MA13   A0A023F3Y2   A0A0M8ZUZ4   D2A047   A0A151K0G5   A0A195BAH3   A0A0L7QQI5   A0A154PEF0   A0A0A9Y6R2   A0A1B6EGG0   A0A1B6I6P9   A0A2P8ZM23   T1JEW7   A0A0P4W2G4   A0A1B6HKS4   A0A2R7W2B3   A0A1Q3FEG8   A0A1B0CLS7   A0A3Q3E9A5   A0A182GJK0   B0WQT7   A0A3P9PTQ9   A0A1I8QFA3   M3ZQ84   A0A3Q1IA97   A0A3B5LUS0   A0A182FR54   Q16PL8   A0A3B3U7A3   A0A1S3JW12   A0A087XWE3   A0A084VTN2   A0A3P8VKB6   A0A1W4Y7L5   A0A1A8NXZ4   C3Z1Q7   A0A3B5AEE8   A0A3B4CVT6   A0A2Y9E4X8   A0A3Q3MQX9   A0A1A8DV13   A0A1A8A0H9   A0A1A8ETH7   I3NC26   A0A182QKB4   A0A3B4U471   H9G5D6   A0A1A8K0W6   Q7ZWP8   T1DLK2   A0A452IL90   A0A2I0M851   A0A2D0PN12   A0A3Q2ZX36   W5K0Z9   R7VUT8   A0A1U7RK97   A0A1A7XLQ2   A0A146P593   A0A1V4JRS6   A0A3B4XNN5   A0A2I0UA86   Q5ZK71   A0A0K8RW07   A0A0P4VX55   A0A3Q2Y361   A0A182J079   A0A0L0CFQ1   H2MSA1   F1QNX4   G3TL95   A0A3B3SRF3   A0A3P9KYT8   A0A2K5E7V3   A0A224YXE5   A0A2I4CSL9  

PDB

5GVE     E-value=0,     Score=2243

KEGG

101735905    K03165 DNA topoisomerase III [EC:5.6.2.1] | (RefSeq) DNA topoisomerase 3-beta-1

PATHWAY

03440    Homologous recombination - Bombyx mori (domestic silkworm)
03460    Fanconi anemia pathway - Bombyx mori (domestic silkworm)

GO

GO:0003677   GO:0003916   GO:0006265   GO:0003917   GO:0005634   GO:0000793   GO:0007059   GO:0016887   GO:0030145   GO:0006412   GO:0016876   GO:0043039  

PANTHER

PTHR11390  

Length:

855

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.02559

Exp number, first 60 AAs:

0.00088

Total prob of N-in:

0.00020

outside

1  -  855

 

 

Pi

245.107427

Theta

160.621689

Tajima's D

1.246577

CLR

0.838934

CSRT

0.728863556822159

Interpretation

Uncertain