Gene

KWMTBOMO08152

Pre Gene Modal

BGIBMGA014373

Annotation

PREDICTED:_protein_toll-like_[Bombyx_mori]

Location

Bomo_Chr14(+):91176-97571      View in EpiBrowser

Full name

Protein toll      

Location in the cell

Nuclear   Reliability : 1.08 PlasmaMembrane   Reliability : 1.192

CDS

ATGGACCTCTGCCCCGGCAACTCCAACGCACACGTCGGCTCCGGCACTTCCCCGCTAAAAGCGCTGACGGAGTTGGATGAGCTGAGACTGGGTCACACGGGTGTGTCTCACGTGTGCAGCGACTGGCGGACCTCCATGAAGCAACTCAGTCTTCTCGATCTCTCCTACACAAACATCACCTCCCTGTCGTACCTGGATTTCCACTACGAGCGCTCTGGATCCAAAAAGCTGACCGTGAACTTCAACGGCACGCCCGTCAGCGAGCTCACCGTCGACGCGGGGAACTACAAGTCTGTGATCGCCGACCTGGCATCTGCATCGGTCAAGATAATATTGAGAGCAACTTTACCGTGCGACTGCTTGGACTACTGGACGGCACTGGCGTTCGGGAACCTCAGCACTCAAGCACAAGATTCCGTGGAGCTCCTGTGTAGGGAGGAGGAGCGTGGTTCACTAGCGGACGCGTTGGAACGGGAATCTCTGGAGTGTGCGGAATCGAGCCTGTGTGAGGACCAGGAAGGCTGCACCTGTTCCGTACGCCCTGACCTCTCGCATGGTGTGGTGGCGACCGCGTCATGTGAGGGCGCGGGTCTGGACGAAATGCCATCGCGTCTGAAGTTCAAAGAGGCTCCCGCGTGGCGCTTAATGCTCGCCCACAACCACATCCAGACCGTTCGGTTTCAGGATCTGCCCGACACGATATTGGAGTTGGACCTGAGAAACAACTCTCTGCATAGATTGGACGGCCCGACTGCTTCGAAGTTATCAACTGTCCCTCTGTGGATCGCCAACAATTCGCTGGACTGCACATGTCACGGGTACGACTTGGTGACGAACCTCGAACTGTGTGTGCTCGACATGGAGCAAGCGACGTGTGACGACGGGACCCCTCTAATTGAAGCACAGATCAACGATCCCACTGAATGCAGTTCGTTTGTAACGGTCACCGTCTCTTTGATCAGCGCGCTGGCTTTGTTGGCAGTGATGGTCGCAGTTACAGCCGGCTGCGGGATGCGACCCGAGATGCGGCTGAGAGTTAAGGTGCTGCTGCTGCGACTGGGCTGGCTGCCACGGCGGCCGGAGCCGGACGACGGGCGTCGGTTCGATGCGTTCGTATCGTACGCGCACGAGGACGAGGCGGTGGTGGAAGAGCTCGTGAAGCGGCTTGAGGTGGGGCACGGCTACCGGCTGTGCCTGCACTACCGCGACTGGCCGCCGGGCGAGTGGATCCACGTGCAGATCGCGGCCTCGGTGCAAGCGGCGCGCCGCACCCTCATCGTAGTCTCGCGGCACTTCCTGCGCTCCAAGTGGGCGCGCCAAGAGTTCCGCCAGGCACACGCGGCCGCGCTGCGTGACGTCACACCGCGCCTCGTGCTGCTGTTCCTCGAGCCGCCGCACCGCTTGCCTCTCGACGCCGAGCTGCGCTCCTACATTAGGATCAACACCTACCTGCTCTGGACCGACCCCTGGTTCTGGCACAAACTAAAGCTAGCCCTGCCGCCCCCGCGCCTGCTCCCCACCCCGTTCGAGGATGTCTCCGTTAATAAGGCGGAGAGCTCCCCTCTACAAGTAGCGCCACGCCCGCAGAGTGCGTGA

Protein

MDLCPGNSNAHVGSGTSPLKALTELDELRLGHTGVSHVCSDWRTSMKQLSLLDLSYTNITSLSYLDFHYERSGSKKLTVNFNGTPVSELTVDAGNYKSVIADLASASVKIILRATLPCDCLDYWTALAFGNLSTQAQDSVELLCREEERGSLADALERESLECAESSLCEDQEGCTCSVRPDLSHGVVATASCEGAGLDEMPSRLKFKEAPAWRLMLAHNHIQTVRFQDLPDTILELDLRNNSLHRLDGPTASKLSTVPLWIANNSLDCTCHGYDLVTNLELCVLDMEQATCDDGTPLIEAQINDPTECSSFVTVTVSLISALALLAVMVAVTAGCGMRPEMRLRVKVLLLRLGWLPRRPEPDDGRRFDAFVSYAHEDEAVVEELVKRLEVGHGYRLCLHYRDWPPGEWIHVQIAASVQAARRTLIVVSRHFLRSKWARQEFRQAHAAALRDVTPRLVLLFLEPPHRLPLDAELRSYIRINTYLLWTDPWFWHKLKLALPPPRLLPTPFEDVSVNKAESSPLQVAPRPQSA

Description

Receptor for the cleaved activated form of spz, spaetzle C-106 (PubMed:12872120). Binding to spaetzle C-106 activates the Toll signaling pathway and induces expression of the antifungal peptide drosomycin (PubMed:12872120, PubMed:8808632, PubMed:10973475). Component of the extracellular signaling pathway that establishes dorsal-ventral polarity in the embryo (PubMed:3931919). Promotes heterophilic cellular adhesion (PubMed:2124970). Involved in synaptic targeting of motoneurons RP5 and V to muscle 12 (M12); functions as a repulsive cue inhibiting motoneuron synapse formation on muscle 13 (M13) to guide RP5 and V to the neighboring M12, where its expression is repressed by tey (PubMed:20504957). May also function in embryonic neuronal survival and the synaptic targeting of SNa motoneurons (PubMed:19018662).

Subunit

In the absence of ligand, forms a low-affinity disulfide-linked homodimer (PubMed:24733933). In the presence of ligand, crystal structures show one Tl molecule bound to a spaetzle C-106 homodimer (PubMed:24282309, PubMed:24733933). However, the active complex probably consists of two Tl molecules bound to a spaetzle C-106 homodimer (PubMed:24282309, PubMed:24733933). This is supported by in vitro experiments which also show binding of the spaetzle C-106 dimer to 2 Tl receptors (PubMed:12872120). Ligand binding induces conformational changes in the extracellular domain of Tl (PubMed:24282309). This may enable a secondary homodimerization interface at the C-terminus of the Tl extracellular domain (PubMed:24282309).

Similarity

Belongs to the Toll-like receptor family.

Keywords

3D-structure   Cell adhesion   Cell membrane   Complete proteome   Cytoplasm   Developmental protein   Disulfide bond   Glycoprotein   Immunity   Innate immunity   Leucine-rich repeat   Membrane   Polymorphism   Receptor   Reference proteome   Repeat   Signal   Transmembrane   Transmembrane helix  

Feature

chain  Protein toll

Uniprot

H9JXV5   H9JXV2   A0A2H1W8Q3   A4GVU0   A0A0L7LPX8   A0A0M8ZY10   + More
A0A0P4VMK0   T1HYZ2   A0A0L7QLM2   A0A224X644   A0A195F0C9   A0A026W7S0   A0A346RAF5   A0A151JAL5   E2BBY4   F4W4T1   A0A158P3M6   A0A310S7W9   A0A154NYN7   A1C1P2   D2CGM6   A0A2J7QHL9   A0A067R3H7   E5D2L0   A0A088AG15   B2WSC9   A0A2J7QHL1   D7EZ50   B4K8Y1   A0A232FI58   A0A2A3EQD4   K7IRA7   Q8MQU7   B5BRC1   A0A0A9XC66   A0A146L775   W8PVP4   A0A410SF83   A0A0D3QDL8   A0A182GPG4   A0A0D3QDD7   B3P637   V9I193   A0A2C9H6H6   B4LWB8   A0A182IDR8   B4PSC7   Q6V666   Q24622   Q6V669   B4QWS2   A0A1S4FH10   Q6V672   Q56RA0   A0A1B0CQQ9   Q6V670   A0A3B0K7X3   A0A2C9GWS9   A0A0M5J9C0   A0A182UFK6   A0A0D3QD03   A0A0D3QDC7   Q8WRE5   Q6V495   A0A0D3QDL3   A0A0D3QCR2   A0A0D3QCQ2   Q6V673   Q6V667   Q56R98   A0A0B4KHY4   T2GGK5   P08953   Q29B96   W8PWC1   A0A0D3QCQ7   Q8MQU9   A0A182Y4Y2   A0A182PVM9   K7ITQ2   A0A1L8E2X4   Q17F73   A0A1I8PSW5   A0A1S4F4W7   A0A182M8X8   Q6V671   J9JPZ4   A0A182XNF0   A0A182KC65  

Pubmed

19121390    17606296    26227816    27129103    24508170    30249741    + More
29910977    20798317    21719571    21347285    17056116    24845553    18321728    17994087    28648823    20075255    12542637    25401762    26823975    25552603    26483478    24398262    18057021    17550304    12930753    8112609    17510324    15545653    16547107    12213252    12364791    14747013    17210077    10731132    12537568    12537572    12537573    12537574    16110336    17569856    17569867    2449285    12537569    3931919    2124970    1879347    8808632    10973475    12617819    12872120    15373972    19018662    22464169    20504957    23596340    24282309    23245851    24733933    15632085    25244985   

EMBL

BABH01040363    BABH01040364    BABH01040360    BABH01040361    ODYU01007053    SOQ49485.1    + More
EF442782    ABO21763.1    JTDY01000372    KOB77487.1    KQ435801    KOX73247.1    GDKW01003048    JAI53547.1    ACPB03009442    KQ414914    KOC59528.1    GFTR01008481    JAW07945.1    KQ981880    KYN33933.1    KK107356    QOIP01000010    EZA52048.1    RLU17569.1    MH243021    AXS59155.1    KQ979262    KYN22067.1    GL447175    EFN86808.1    GL887553    EGI70800.1    ADTU01008265    KQ775195    OAD52269.1    KQ434773    KZC03990.1    DQ923424    ABK58729.1    EF117252    ABO38434.1    NEVH01013973    PNF28084.1    KK852931    KDR13629.1    GU014556    ADK55066.1    EF407561    ABQ59330.1    PNF28085.1    EU433386    ACC68670.1    CH933806    EDW16578.1    NNAY01000160    OXU30431.1    KZ288197    PBC33714.1    AY124197    AAM97776.1    AB385869    BAG68890.1    GBHO01026374    GBHO01026373    GBHO01026372    GBHO01026370    JAG17230.1    JAG17231.1    JAG17232.1    JAG17234.1    GDHC01015553    GDHC01008551    JAQ03076.1    JAQ10078.1    KJ188411    AHL39101.1    MH178390    QAT92918.1    KP274553    AJC98699.1    JXUM01078342    JXUM01078343    KP274549    AJC98695.1    CH954182    EDV53507.1    JF895474    AEI25533.1    CH940650    EDW67652.2    KRF83383.1    APCN01004779    APCN01004780    CM000160    EDW98589.2    AY349924    AAQ64875.1    L25390    AAA28942.1    AY349921    AAQ64872.1    CM000364    EDX14589.1    AY349918    AAQ64869.1    AY754607    AAX13182.1    AJWK01023878    AY349920    AAQ64871.1    OUUW01000005    SPP81101.1    CP012526    ALC46724.1    KP274543    KP274546    AJC98689.1    AJC98692.1    KP274533    KP274534    KP274535    KP274536    KP274537    KP274538    KP274539    KP274540    KP274541    KP274544    KP274551    AJC98679.1    AJC98680.1    AJC98681.1    AJC98682.1    AJC98683.1    AJC98684.1    AJC98685.1    AJC98686.1    AJC98687.1    AJC98690.1    AJC98697.1    AF444780    AAAB01008811    AAL37901.1    EAA45376.1    AY352264    AAQ65064.1    KP274545    KP274548    KP274550    AJC98691.1    AJC98694.1    AJC98696.1    KP274552    AJC98698.1    KP274542    AJC98688.1    AY349917    AAQ64868.1    AY349922    AY349923    AAQ64873.1    AAQ64874.1    AY754609    AAX13184.1    AE014297    AGB96375.1    BT150335    AGW25617.1    M19969    BT031153    AY349649    AY349650    AY349651    AY349652    AY349653    AY349654    AY349655    AY121616    CM000070    EAL27102.2    KJ188410    AHL39100.1    KP274547    AJC98693.1    AY124195    AAM97774.1    GFDF01000994    JAV13090.1    CH477273    EAT45212.2    AXCM01000253    AY349919    AAQ64870.1    ABLF02029217   

Proteomes

UP000005204    UP000037510    UP000053105    UP000015103    UP000053825    UP000078541    + More
UP000053097    UP000279307    UP000078492    UP000008237    UP000007755    UP000005205    UP000076502    UP000235965    UP000027135    UP000005203    UP000009192    UP000215335    UP000242457    UP000002358    UP000075903    UP000069940    UP000008711    UP000075920    UP000008792    UP000075840    UP000002282    UP000000304    UP000092461    UP000268350    UP000075900    UP000092553    UP000075902    UP000007062    UP000000803    UP000001819    UP000076408    UP000075885    UP000008820    UP000095300    UP000075883    UP000007819    UP000076407    UP000075881   

PRIDE

H9JXV5    H9JXV2    T2GGK5    P08953   

Pfam

PF13676   TIR_2        + More
PF13855   LRR_8
PF01582   TIR
PF13306   LRR_5
PF00560   LRR_1
PF01462   LRRNT
PF01463   LRRCT

Interpro

IPR035897   Toll_tir_struct_dom_sf        + More
IPR032675   LRR_dom_sf       
IPR000157   TIR_dom       
IPR001611   Leu-rich_rpt       
IPR003591   Leu-rich_rpt_typical-subtyp       
IPR000483   Cys-rich_flank_reg_C       
IPR026906   LRR_5       
IPR000372   LRRNT       

SUPFAM

SSF52200   SSF52200       

Gene 3D

3.40.50.10140     3.80.10.10    

ProteinModelPortal

H9JXV5   H9JXV2   A0A2H1W8Q3   A4GVU0   A0A0L7LPX8   A0A0M8ZY10   + More
A0A0P4VMK0   T1HYZ2   A0A0L7QLM2   A0A224X644   A0A195F0C9   A0A026W7S0   A0A346RAF5   A0A151JAL5   E2BBY4   F4W4T1   A0A158P3M6   A0A310S7W9   A0A154NYN7   A1C1P2   D2CGM6   A0A2J7QHL9   A0A067R3H7   E5D2L0   A0A088AG15   B2WSC9   A0A2J7QHL1   D7EZ50   B4K8Y1   A0A232FI58   A0A2A3EQD4   K7IRA7   Q8MQU7   B5BRC1   A0A0A9XC66   A0A146L775   W8PVP4   A0A410SF83   A0A0D3QDL8   A0A182GPG4   A0A0D3QDD7   B3P637   V9I193   A0A2C9H6H6   B4LWB8   A0A182IDR8   B4PSC7   Q6V666   Q24622   Q6V669   B4QWS2   A0A1S4FH10   Q6V672   Q56RA0   A0A1B0CQQ9   Q6V670   A0A3B0K7X3   A0A2C9GWS9   A0A0M5J9C0   A0A182UFK6   A0A0D3QD03   A0A0D3QDC7   Q8WRE5   Q6V495   A0A0D3QDL3   A0A0D3QCR2   A0A0D3QCQ2   Q6V673   Q6V667   Q56R98   A0A0B4KHY4   T2GGK5   P08953   Q29B96   W8PWC1   A0A0D3QCQ7   Q8MQU9   A0A182Y4Y2   A0A182PVM9   K7ITQ2   A0A1L8E2X4   Q17F73   A0A1I8PSW5   A0A1S4F4W7   A0A182M8X8   Q6V671   J9JPZ4   A0A182XNF0   A0A182KC65  

PDB

1O77     E-value=2.42274e-14,     Score=193

KEGG

101736405    K18809 protein toll | (RefSeq) protein toll-like

GO

GO:0007165   GO:0016021   GO:0045087   GO:0032991   GO:0004896   GO:0004888   GO:0035208   GO:0009897   GO:1902875   GO:0005769   GO:0007526   GO:0040015   GO:0007155   GO:0032154   GO:0007507   GO:0008063   GO:0009950   GO:0046627   GO:0045944   GO:0016201   GO:0002229   GO:0005887   GO:0019955   GO:0061760   GO:0030308   GO:0042802   GO:0009611   GO:0007416   GO:0070976   GO:0009986   GO:0002225   GO:0002804   GO:0050830   GO:0005886   GO:0005515   GO:0005488   GO:0016791   GO:0005634   GO:0030145   GO:0006412   GO:0016876   GO:0043039  

Subcellular location

Cell membrane   In the fat body, detected in puntate structures along the cell periphery. Becomes evenly distributed in the cytoplasm 1 hour after bacterial infection.   With evidence from 11 publications.
Cytoplasm   In the fat body, detected in puntate structures along the cell periphery. Becomes evenly distributed in the cytoplasm 1 hour after bacterial infection.   With evidence from 11 publications.

Length:

531

Number of predicted TMHs:

1

Exp number of AAs in TMHs:

19.68036

Exp number, first 60 AAs:

0.00964

Total prob of N-in:

0.22389

outside

1  -  312

TMhelix

313  -  335

inside

336  -  531

 

 

Pi

178.993261

Theta

171.854406

Tajima's D

1.167203

CLR

0.366363

CSRT

0.709414529273536

Interpretation

Uncertain