Gene

KWMTBOMO08127

Pre Gene Modal

BGIBMGA001093

Annotation

PREDICTED:_lipoyl_synthase?_mitochondrial_[Papilio_machaon]

Location

Bomo_Chr13(-):17116622-17124160      View in EpiBrowser

Full name

Lipoyl synthase, mitochondrial       + More
Lipoyl synthase 2, mitochondrial      
Lipoyl synthase 1, mitochondrial      

Alternative Name

Lipoate synthase
Lipoic acid synthase
Lipoate synthase 2
Lipoic acid synthase 2
Lipoate synthase 1
Lipoic acid synthase 1

Location in the cell

Cytoplasmic   Reliability : 1.511 Mitochondrial   Reliability : 2.096

CDS

ATGATTCGCAACACAATTAAAAAGTATAAAGTCACCAAACATTTGGACTTTCCAAGATACAAACACTCAAATAAGTTAGATGCAATCAGAGAAAAATTAAAAGAGGGTCCGAATCTAACCGATTTTATATCTGAAGATCGTCCAAAAAATTGGGATGAATATCAAGGCAAGCTGAAAAGGGAAAAAGGTGAATCCGAGAGACTTAGGTTACCGCCATGGTTGAAAACTACTATACCCACAGGTTCAAAATTCAATGAAATAAAACAGCAGTTACGTAGTTTGAAGCTTAGCACTGTTTGCGAAGAAGCAAGGTGTCCTAATATTGGAGAATGTTGGAGCGGAGGAAAACACGGTGCATCAACTGCTACCATCATGTTAATGGGAGATACCTGTACAAGAGGCTGCATGTTCTGCTCAGTGAAGACATCAAGGAATCCACCACCTCTGGACCCAGAGGAGCCAAGGAACACTGCACATGCTATCAACCAGTGGGGTGTCGGATATATTGTACTTACCTCAGTTGATAGAGACGACTTACCAGATGGAGGTTCGCTGCATTTTGCCGAAACAGTTAAAGAGATCAAAAAGCTCAACAAGGACATCCTAGTGGAGTGCCTGGTGCCGGACTTCCGAGCCGTCCGGGGCTGCGTGGCGCGCGTGGCCTCCAGCGGCCTCGACGTGTTCGCGCACAACGTCGAGACCGTCGAGAGACTCACGCCCTTCGTGCGTGACCCCAGGGCGGGGTACCGCCAAACGTTGAAAGTTTTACAAATGGCCAAAGAAATAAATCCTGATTTAATAACGAAGTCGTCCATCATGCTGGGACTCGGCGAGACCGACGCGCAAGTGGAGCAGACCATGAAAGATCTACGCGAGGCGGGAGTGGACTGCGTAACGCTGGGCCAGTACATGCAGCCCACGAAGAAGCACCTCAAGGTGTTCGAGTACGTGACCCCCGCCCGGTTCCAGCAGTGGGAGGCGCGCGGCCGCCAGCTCGGCTTCCTGTACGTGGCCAGCGGAGCCCTGGTGCGCTCGTCCTACAGGGCCGGAGAGTTCTTCATCAGCGCCGTGCTGCGGGACAGGAAGGTCAACTCGCAATGA

Protein

MIRNTIKKYKVTKHLDFPRYKHSNKLDAIREKLKEGPNLTDFISEDRPKNWDEYQGKLKREKGESERLRLPPWLKTTIPTGSKFNEIKQQLRSLKLSTVCEEARCPNIGECWSGGKHGASTATIMLMGDTCTRGCMFCSVKTSRNPPPLDPEEPRNTAHAINQWGVGYIVLTSVDRDDLPDGGSLHFAETVKEIKKLNKDILVECLVPDFRAVRGCVARVASSGLDVFAHNVETVERLTPFVRDPRAGYRQTLKVLQMAKEINPDLITKSSIMLGLGETDAQVEQTMKDLREAGVDCVTLGQYMQPTKKHLKVFEYVTPARFQQWEARGRQLGFLYVASGALVRSSYRAGEFFISAVLRDRKVNSQ

Description

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.

Catalytic Activity

[[Fe-S] cluster scaffold protein carrying a second [4Fe-4S](2+) cluster] + 4 H(+) + N(6)-octanoyl-L-lysyl-[protein] + 2 oxidized [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine = (R)-N(6)-dihydrolipoyl-L-lysyl-[protein] + 2 5'-deoxyadenosine + [[Fe-S] cluster scaffold protein] + 4 Fe(3+) + 2 hydrogen sulfide + 2 L-methionine + 2 reduced [2Fe-2S]-[ferredoxin]

Cofactor

[4Fe-4S] cluster

Miscellaneous

This protein may be expected to contain an N-terminal transit peptide but none has been predicted.

Similarity

Belongs to the radical SAM superfamily. Lipoyl synthase family.

Keywords

4Fe-4S   Complete proteome   Iron   Iron-sulfur   Metal-binding   Mitochondrion   Reference proteome   S-adenosyl-L-methionine   Transferase  

Feature

chain  Lipoyl synthase, mitochondrial

Uniprot

H9IV13   A0A2W1BVQ9   A0A2H1W2A8   A0A2A4K2E3   A0A194RF73   A0A212EVM3   + More
A0A194QC88   A0A1B0CUY4   A0A1B0DAS7   A0A1B0GHL2   A0A1L8DZK8   A0A034VVH3   A0A0A1XTI8   A0A1L8E007   W8C6K5   A0A0L0C8M5   B3M996   A0A1J1HMX6   Q7JQW6   A0A0J9UQ26   B4IAA7   B3NIL9   A0A034VZ06   B4PF83   B4IUG3   B4KYY0   A0A0K8UJQ8   A0A182V3G6   Q7Q8T4   A0A182TJ34   A0A1S4H5C6   A0A1W4VT93   B4J3F3   A0A1I8M2N2   B4LD74   D3TM61   B4MXR6   A0A182HFR5   A0A182X1V1   A0A182Q8B3   T1PG22   A0A1B0ARA4   A0A182YA32   Q16W22   A0A1A9V2Z6   A0A1W4WHT1   A0A182M172   A0A1A9YL26   A0A3B0KTW4   A0A1I8PGW5   A0A1Q3FH57   A0A182G9I5   A0A1A9WBN8   Q2LYK1   A0A2M4BQ31   A0A1L8EAX8   A0A2M4BPU8   B0WAU6   A0A182S4V7   A0A182FUK0   A0A1B6CVV3   A0A1L8EB96   A0A2M4ABH2   A0A182P8H4   A0A2M3Z761   A0A2M4ABI1   A0A2M3Z708   A0A067QH25   D1MLN9   W5JDI3   A0A182JIE6   A0A2J7RFZ8   A0A084VKW9   A0A1B6HCJ6   D2A066   E0VJV1   N6UWX8   A0A1B6FBG2   A0A336MQE3   N6TJ48   U4UYV1   E2AE87   A0A154NXC3   A0A182MYD5   A0A182VTB7   A0A2A3EHN6   A0A088ATF3   A0A310SDJ5   A0A336M347   A0A0N0BGQ2   A0A0P4WJ23   A0A0V0GDZ5   A0A1D2N8K3   A0A224XQT4   A0A026W4C4   A0A2S2QG76   A0A3R7LXJ6   A0A3L8D2I1   E2BFG7   A0A023F8H6  

EC Number

2.8.1.8

Pubmed

19121390    28756777    26354079    22118469    25348373    25830018    + More
24495485    26108605    17994087    10731132    12537572    12537569    22936249    12364791    25315136    20353571    25244985    17510324    26483478    15632085    24845553    20920202    20920257    23761445    24438588    18362917    19820115    20566863    23537049    20798317    27289101    24508170    30249741    25474469   

EMBL

BABH01000069    BABH01000070    KZ149891    PZC79069.1    ODYU01005882    SOQ47230.1    + More
NWSH01000278    PCG77812.1    KQ460297    KPJ16237.1    AGBW02012175    OWR45536.1    KQ459439    KPJ01051.1    AJWK01029837    AJVK01013389    AJWK01005316    GFDF01002218    JAV11866.1    GAKP01011636    JAC47316.1    GBXI01000169    JAD14123.1    GFDF01002219    JAV11865.1    GAMC01004189    JAC02367.1    JRES01000760    KNC28600.1    CH902618    CVRI01000012    CRK89405.1    AE014296    AY058557    BT003750    CM002912    KMZ00786.1    CH480826    CH954178    GAKP01011635    JAC47317.1    CM000159    CH891902    CH933809    GDHF01025571    JAI26743.1    AAAB01008933    EAA09970.4    CH916366    CH940647    EDW69955.2    CCAG010010280    EZ422513    ADD18789.1    CH963876    APCN01005567    AXCN02002053    KA647624    AFP62253.1    JXJN01002297    CH477577    AXCM01009715    OUUW01000012    SPP87348.1    GFDL01008158    JAV26887.1    JXUM01049228    KQ561596    KXJ78052.1    CH379069    GGFJ01005890    MBW55031.1    GFDG01002911    JAV15888.1    GGFJ01005891    MBW55032.1    DS231875    GEDC01019965    JAS17333.1    GFDG01002910    JAV15889.1    GGFK01004812    MBW38133.1    GGFM01003590    MBW24341.1    GGFK01004822    MBW38143.1    GGFM01003540    MBW24291.1    KK853449    KDR07551.1    GU196316    ACZ26290.1    ADMH02001479    ETN62407.1    NEVH01004406    PNF39775.1    ATLV01014310    KE524956    KFB38613.1    GECU01035374    JAS72332.1    KQ971338    EFA02475.1    DS235229    EEB13657.1    APGK01003385    KB735241    ENN83412.1    GECZ01022526    JAS47243.1    UFQS01001558    UFQT01001558    SSX11436.1    SSX31003.1    APGK01023998    KB740528    ENN80469.1    KB632431    ERL95510.1    GL438827    EFN68320.1    KQ434777    KZC04212.1    KZ288253    PBC30974.1    KQ797156    OAD46987.1    UFQT01000450    SSX24510.1    KQ435771    KOX75142.1    GDRN01041269    JAI67097.1    GECL01000610    JAP05514.1    LJIJ01000146    ODN01582.1    GFTR01005504    JAW10922.1    KK107429    EZA50935.1    GGMS01007307    MBY76510.1    QCYY01003931    ROT61915.1    QOIP01000014    RLU14715.1    GL448037    EFN85489.1    GBBI01001077    JAC17635.1   

Proteomes

UP000005204    UP000218220    UP000053240    UP000007151    UP000053268    UP000092461    + More
UP000092462    UP000037069    UP000007801    UP000183832    UP000000803    UP000001292    UP000008711    UP000002282    UP000009192    UP000075903    UP000007062    UP000075902    UP000192221    UP000001070    UP000095301    UP000008792    UP000092444    UP000007798    UP000075840    UP000076407    UP000075886    UP000092460    UP000076408    UP000008820    UP000078200    UP000192223    UP000075883    UP000092443    UP000268350    UP000095300    UP000069940    UP000249989    UP000091820    UP000001819    UP000002320    UP000075900    UP000069272    UP000075885    UP000027135    UP000000673    UP000075880    UP000235965    UP000030765    UP000007266    UP000009046    UP000019118    UP000030742    UP000000311    UP000076502    UP000075884    UP000075920    UP000242457    UP000005203    UP000053105    UP000094527    UP000053097    UP000283509    UP000279307    UP000008237   

PRIDE

H9IV13    B3M996    Q7JQW6    B4IAA7    B3NIL9    B4PF83    + More
B4IUG3    B4KYY0    B4J3F3    B4MXR6    Q2LYK1    B0WAU6   

Pfam

PF04055   Radical_SAM        + More
PF16881   LIAS_N
PF10169   Laps
PF13906   AA_permease_C
PF13520   AA_permease_2
PF06331   Tfb5

Interpro

IPR003698   Lipoyl_synth        + More
IPR007197   rSAM       
IPR006638   Elp3/MiaB/NifB       
IPR013785   Aldolase_TIM       
IPR031691   LIAS_N       
IPR018784   LAPS18-like       
IPR029485   CAT_C       
IPR002293   AA/rel_permease1       
IPR009400   TFIIH_TTDA/Tfb5       
IPR035935   TFB5-like_sf       

SUPFAM

SSF142897   SSF142897       

Gene 3D

3.20.20.70     3.30.70.1220    

ProteinModelPortal

H9IV13   A0A2W1BVQ9   A0A2H1W2A8   A0A2A4K2E3   A0A194RF73   A0A212EVM3   + More
A0A194QC88   A0A1B0CUY4   A0A1B0DAS7   A0A1B0GHL2   A0A1L8DZK8   A0A034VVH3   A0A0A1XTI8   A0A1L8E007   W8C6K5   A0A0L0C8M5   B3M996   A0A1J1HMX6   Q7JQW6   A0A0J9UQ26   B4IAA7   B3NIL9   A0A034VZ06   B4PF83   B4IUG3   B4KYY0   A0A0K8UJQ8   A0A182V3G6   Q7Q8T4   A0A182TJ34   A0A1S4H5C6   A0A1W4VT93   B4J3F3   A0A1I8M2N2   B4LD74   D3TM61   B4MXR6   A0A182HFR5   A0A182X1V1   A0A182Q8B3   T1PG22   A0A1B0ARA4   A0A182YA32   Q16W22   A0A1A9V2Z6   A0A1W4WHT1   A0A182M172   A0A1A9YL26   A0A3B0KTW4   A0A1I8PGW5   A0A1Q3FH57   A0A182G9I5   A0A1A9WBN8   Q2LYK1   A0A2M4BQ31   A0A1L8EAX8   A0A2M4BPU8   B0WAU6   A0A182S4V7   A0A182FUK0   A0A1B6CVV3   A0A1L8EB96   A0A2M4ABH2   A0A182P8H4   A0A2M3Z761   A0A2M4ABI1   A0A2M3Z708   A0A067QH25   D1MLN9   W5JDI3   A0A182JIE6   A0A2J7RFZ8   A0A084VKW9   A0A1B6HCJ6   D2A066   E0VJV1   N6UWX8   A0A1B6FBG2   A0A336MQE3   N6TJ48   U4UYV1   E2AE87   A0A154NXC3   A0A182MYD5   A0A182VTB7   A0A2A3EHN6   A0A088ATF3   A0A310SDJ5   A0A336M347   A0A0N0BGQ2   A0A0P4WJ23   A0A0V0GDZ5   A0A1D2N8K3   A0A224XQT4   A0A026W4C4   A0A2S2QG76   A0A3R7LXJ6   A0A3L8D2I1   E2BFG7   A0A023F8H6  

PDB

4U0P     E-value=1.11512e-66,     Score=643

KEGG

101735920    K03644 lipoyl synthase [EC:2.8.1.8] | (RefSeq) lipoyl synthase, mitochondrial

PATHWAY

00785    Lipoic acid metabolism - Bombyx mori (domestic silkworm)
01100    Metabolic pathways - Bombyx mori (domestic silkworm)

GO

GO:0051539   GO:0009249   GO:0016992   GO:0046872   GO:0005739   GO:0009107   GO:0016021   GO:0022857   GO:0006289   GO:0000439   GO:0006355   GO:0003824   GO:0051536   GO:0015914   GO:0006412   GO:0016876   GO:0043039  

PANTHER

PTHR10949  

Subcellular location

Mitochondrion  

Length:

366

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.16457

Exp number, first 60 AAs:

0

Total prob of N-in:

0.17449

outside

1  -  366

 

 

Pi

16.821846

Theta

14.709266

Tajima's D

0.074601

CLR

0.346856

CSRT

0.39778011099445

Interpretation

Uncertain