Gene

KWMTBOMO08108

Pre Gene Modal

BGIBMGA001061

Annotation

PREDICTED:_alkylated_DNA_repair_protein_alkB_homolog_8_[Plutella_xylostella]

Location

Bomo_Chr13(+):16026257-16033471      View in EpiBrowser

Full name

Alkylated DNA repair protein alkB homolog 8      

Alternative Name

Probable alpha-ketoglutarate-dependent dioxygenase ABH8
S-adenosyl-L-methionine-dependent tRNA methyltransferase ABH8
tRNA (carboxymethyluridine(34)-5-O)-methyltransferase ABH8

Location in the cell

Cytoplasmic   Reliability : 1.795 Nuclear   Reliability : 1.931

CDS

ATGGAAGACGATAGAAAACATGAGAAGAAACGCAAACGGTTTGCTGCCCGATTGAATACTCTGAAAGGAATTGTTTGTTATGAAGATCCTAGGCCCAATGTGATTCTTTGCAATGTGGGGCAAGCTACAGGATTTGATAAGCAAAAACTGTTGAAACTGCTCACAGAGTACAAGGTAAACACACCATTGCCTAAGTTTGTTGCCGAGAAAGGAGAATCCTACTCATATCTCATGTTTGATAAAGCCGAGAATGCATCACTATTTTATGAGGCATGCAATGGGAAAGCACAGGTAGATGAGAATGGAACAACACTCTATGTGACATTTGTTGAAAATGTTCCGGATACTGATATTATTTGTAAGCATCCAAATCCTAAAGGCTTACATATAATACCCGATTTTCTTACCGAAGATGAAGAGAAGTTATTCCTTAATACCTTTAACATACAACACGTAGAAACAACATTAAAGAACCGTCAAGTCAAACATTATGGTTATGAGTTCAGATATGGCAGCAATGACGTTGACTTGAGTTGCCCATTGCAGGAAAAAATACCAAAGATTTGTAAATTGCTGTGGAGAAGGCTCCAACACTATGGTTACGATCTCGGAGTACCAGTTCAGCTGACTGTTAACAAGTACTTACCTGGGCAAGGTATCCCGTCGCATGTCGACAAACACAGTCCATTTGGAGAAACAATTCTGGCCCTATCACTGGGCTCCAATGTGGTTATGGATTGGAAGCATCACACAGGGAAATATGTGCCAGTTATGGTTGAGGCTAGATCAATGATGATAATGCAAGATGAAGCTAGATATGACTGGCAGCATGGAATACAGCCAAGAATGTGGGACCCAGTCCTTGATGTTCGTTTAATAGATAATGAGAAAGTTAAAGTGATCACATCTGATACTGTACAGAGGGAGATGAGAATCTCCTTGACATTCCGATGGACCAGATGCGGGCCATGTAAATGTGAATACAAGATGTTGTGTGATAGCATTGAACGTAATATTCCTGAGACAATTAGCAATGATGTTGCATCTAACATAGAGGACATACATGTGCACCAGGTATACGAACAAATCGCAGGACACTTCAGCACAACGCGGCACAAGCCTTGGCCCAAAGTTGTAGACTTCATGCAGCACGTGCCGGCTGGATCTATAGTGCTGGACCTTGGCTGTGGCAATGGCAAGAATATACTCAACAGAACAGATATATTGCAGGTAGCCGGTGAAAGGAGCAGCGGTCTACTAGAGGAATGCAAGGGGCTCACGGCCCGGATATCCGGCGCCGACTGCATCCGGTTGGACTTGCTCAACACCGGCCTCAAGGACGAGTGCGCTGATTTCATCATTTGCGTAGCCGTCGTGCACCACTTCAGCACACAGGCACGGCGCCTCCACTCGCTACAAACAATACACCGACTCCTGCGGACCAACGGGCAAGCGCTCATCACGGTGTGGGCCAAGGATCAAACCAAGTCGAGCTACCTGTCCCGGACCCGGGCGCCGCTGCTGGACCGGCACAAACTGACGGTGGGCGGCATCCACTTGCCCGTGCACGAGAACCGCACGCAGTTCCAGCACAAGGATCTGCTGGTGCCCTGGAACCTGCGCAGCAAGGCGCCCCCGCTCCAACAGCCCGACACCACCTTTCTCAGATACTACCACGTCTTCGATGAAGGGGAACTGGATCAGCTCTGCCGAGACGCGCGGCTCGCGATCGTCAGGAGCTTCTACGAGGAAGGCAATTGGTGTGTCGTCTGTATGAAAGTGTAA

Protein

MEDDRKHEKKRKRFAARLNTLKGIVCYEDPRPNVILCNVGQATGFDKQKLLKLLTEYKVNTPLPKFVAEKGESYSYLMFDKAENASLFYEACNGKAQVDENGTTLYVTFVENVPDTDIICKHPNPKGLHIIPDFLTEDEEKLFLNTFNIQHVETTLKNRQVKHYGYEFRYGSNDVDLSCPLQEKIPKICKLLWRRLQHYGYDLGVPVQLTVNKYLPGQGIPSHVDKHSPFGETILALSLGSNVVMDWKHHTGKYVPVMVEARSMMIMQDEARYDWQHGIQPRMWDPVLDVRLIDNEKVKVITSDTVQREMRISLTFRWTRCGPCKCEYKMLCDSIERNIPETISNDVASNIEDIHVHQVYEQIAGHFSTTRHKPWPKVVDFMQHVPAGSIVLDLGCGNGKNILNRTDILQVAGERSSGLLEECKGLTARISGADCIRLDLLNTGLKDECADFIICVAVVHHFSTQARRLHSLQTIHRLLRTNGQALITVWAKDQTKSSYLSRTRAPLLDRHKLTVGGIHLPVHENRTQFQHKDLLVPWNLRSKAPPLQQPDTTFLRYYHVFDEGELDQLCRDARLAIVRSFYEEGNWCVVCMKV

Description

Catalyzes the methylation of 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its methyltransferase domain. Catalyzes the last step in the formation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in target tRNA. Has a preference for tRNA(Arg) and tRNA(Glu), and does not bind tRNA(Lys). Binds tRNA and catalyzes the iron and alpha-ketoglutarate dependent hydroxylation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its dioxygenase domain, giving rise to 5-(S)-methoxycarbonylhydroxymethyluridine; has a preference for tRNA(Gly). Required for normal survival after DNA damage. May inhibit apoptosis and promote cell survival and angiogenesis (By similarity).

Catalytic Activity

carboxymethyluridine(34) in tRNA + S-adenosyl-L-methionine = 5-(2-methoxy-2-oxoethyl)uridine(34) in tRNA + S-adenosyl-L-homocysteine

Cofactor

Fe(2+)

Similarity

Belongs to the alkB family.

Keywords

Complete proteome   Cytoplasm   Iron   Metal-binding   Methyltransferase   Multifunctional enzyme   Nucleus   Oxidoreductase   Reference proteome   RNA-binding   S-adenosyl-L-methionine   Transferase   Zinc  

Feature

chain  Alkylated DNA repair protein alkB homolog 8

Uniprot

H9IUY1   A0A2A4K3N2   A0A437BPC4   A0A2A4K3Z1   A0A194Q7R7   A0A212ETM0   + More
A0A194REG2   A0A1A9ZNI5   A0A1Y1N078   A0A1A9VIH1   A0A2J7QS63   A0A2Z5U609   A0A1B0D934   A0A0A1X0P0   D2A2C2   A0A1W4WS58   A0A1B0BGD9   Q17MR7   A0A182Y9F9   A0A1A9XJ53   A0A2P8ZL88   A0A084WIN4   A0A1I8NHX9   A0A0T6AXC8   A0A067R5V9   A0A0N0BEQ0   W5JWS8   A0A182IX61   A0A2M4CLJ7   A0A182RS07   A0A1B6E4R5   A0A182PYF3   A0A182M3B7   A0A182N2V9   A0A2R7WGB7   A0A336MWL3   E2B3I8   A0A2M4BHG6   A0A2M4BH89   W8BCH1   Q7PZG8   A0A182L530   A0A1S4H7U0   K7J2T9   A0A182XJT8   A0A182UZ32   A0A336M1J7   A0A182TKL7   A0A182QSX5   A0A0L0C1L5   Q9W232   A0A1W4VQN5   Q290H5   N6TXH5   F6ZD81   Q8T9A3   A0A0L7RKL6   A0A088A8Y1   A0A2A3E5W1   B4GC19   B4I868   U4U8C2   B4QHJ3   Q07G10   B4P5H7   A0A096N913   A0A2K5Y7X6   A0A096NCH5   A0A1S3W968   A0A0M5J527   B3NLI7   A0A1S2ZKC4   G1T292   A0A154PK51   K9KC91   F7DYZ5   A0A2I2US18   E2AE04   A0A182KIK7   A0A182WDY8   A0A3B0J0S8   A0A232EJC4   A0A1I8PW84   A0A337S599   A0A3Q7TMR0   E2REE7   A0A3Q7SL51   G3WMU7   A0A182HHJ4   T1J3X4   A0A384C0E5   A0A2Y9DXP8   A0A3Q7WJ91   A0A452TTN1   B3MEC7   E9IFM0   A0A3Q7X508   A0A3Q7XGG4   A0A2K5DGZ9   U3B9R7  

EC Number

1.14.11.-

Pubmed

19121390    26354079    22118469    28004739    26760975    25830018    + More
18362917    19820115    17510324    25244985    29403074    24438588    25315136    24845553    20920257    23761445    20798317    24495485    12364791    20966253    20075255    26108605    10731132    12537568    12537572    12537573    12537574    16110336    17569856    17569867    15632085    17994087    23537049    17495919    22936249    17550304    21993624    19892987    17975172    28648823    16341006    21709235    24813606    21282665    25243066   

EMBL

BABH01000167    NWSH01000210    PCG78373.1    RSAL01000023    RVE52268.1    PCG78372.1    + More
KQ459439    KPJ01040.1    AGBW02012542    OWR44852.1    KQ460297    KPJ16223.1    GEZM01019409    JAV89646.1    NEVH01011878    PNF31428.1    FX985781    BBA93668.1    AJVK01012889    GBXI01009992    JAD04300.1    KQ971338    EFA02043.1    JXJN01013833    CH477204    EAT47955.1    PYGN01000024    PSN57265.1    ATLV01023941    KE525347    KFB50078.1    LJIG01022609    KRT79652.1    KK852866    KDR14734.1    KQ435827    KOX71948.1    ADMH02000120    ETN67745.1    GGFL01002032    MBW66210.1    GEDC01004375    JAS32923.1    AXCM01000907    KK854778    PTY18646.1    UFQT01002849    SSX34135.1    GL445346    EFN89740.1    GGFJ01003260    MBW52401.1    GGFJ01003261    MBW52402.1    GAMC01011852    JAB94703.1    AAAB01008986    EAA00113.2    UFQT01000416    SSX24115.1    AXCN02001090    JRES01001012    KNC26152.1    AE013599    AAF46867.1    CM000071    EAL25387.2    APGK01051339    KB741189    ENN73061.1    AY069858    AAL40003.1    KQ414573    KOC71296.1    KZ288379    PBC26559.1    CH479181    EDW32362.1    CH480824    EDW56793.1    KB632216    ERL90169.1    CM000362    CM002911    EDX08222.1    KMY95817.1    CR855607    CM000158    EDW91808.1    AHZZ02007567    AHZZ02007568    AHZZ02007569    AHZZ02007570    AHZZ02007571    AHZZ02007572    CP012524    ALC42006.1    CH954179    EDV54903.1    AAGW02009189    AAGW02009190    AAGW02009191    KQ434943    KZC12222.1    JL620619    AEP99344.1    AANG04000369    GL438827    EFN68237.1    OUUW01000001    SPP74337.1    NNAY01004036    OXU18466.1    AAEX03003519    AEFK01115055    AEFK01115056    AEFK01115057    AEFK01115058    AEFK01115059    APCN01002417    JH431832    CH902619    EDV36533.1    GL762865    EFZ20637.1    GAMT01009532    JAB02329.1   

Proteomes

UP000005204    UP000218220    UP000283053    UP000053268    UP000007151    UP000053240    + More
UP000092445    UP000078200    UP000235965    UP000092462    UP000007266    UP000192223    UP000092460    UP000008820    UP000076408    UP000092443    UP000245037    UP000030765    UP000095301    UP000027135    UP000053105    UP000000673    UP000075880    UP000075900    UP000075885    UP000075883    UP000075884    UP000008237    UP000007062    UP000075882    UP000002358    UP000076407    UP000075903    UP000075902    UP000075886    UP000037069    UP000000803    UP000192221    UP000001819    UP000019118    UP000002280    UP000053825    UP000005203    UP000242457    UP000008744    UP000001292    UP000030742    UP000000304    UP000008143    UP000002282    UP000028761    UP000233140    UP000079721    UP000092553    UP000008711    UP000001811    UP000076502    UP000002281    UP000011712    UP000000311    UP000075881    UP000075920    UP000268350    UP000215335    UP000095300    UP000286640    UP000002254    UP000007648    UP000075840    UP000261680    UP000291021    UP000248480    UP000286642    UP000007801    UP000233020   

PRIDE

H9IUY1    Q9W232    Q8T9A3   

Pfam

PF13532   2OG-FeII_Oxy_2        + More
PF08241   Methyltransf_11
PF00076   RRM_1
PF13649   Methyltransf_25
PF09004   DUF1891

Interpro

IPR005123   Oxoglu/Fe-dep_dioxygenase        + More
IPR013216   Methyltransf_11       
IPR027450   AlkB-like       
IPR029063   SAM-dependent_MTases       
IPR032863   ALKBH8       
IPR037151   AlkB-like_sf       
IPR012677   Nucleotide-bd_a/b_plait_sf       
IPR035979   RBD_domain_sf       
IPR000504   RRM_dom       
IPR034256   ALKBH8_RRM       
IPR041698   Methyltransf_25       
IPR015095   AlkB_hom8_N       

SUPFAM

SSF53335   SSF53335        + More
SSF54928   SSF54928       

Gene 3D

2.60.120.590     3.30.70.330    

CDD

cd12431  RRM_ALKBH8

ProteinModelPortal

H9IUY1   A0A2A4K3N2   A0A437BPC4   A0A2A4K3Z1   A0A194Q7R7   A0A212ETM0   + More
A0A194REG2   A0A1A9ZNI5   A0A1Y1N078   A0A1A9VIH1   A0A2J7QS63   A0A2Z5U609   A0A1B0D934   A0A0A1X0P0   D2A2C2   A0A1W4WS58   A0A1B0BGD9   Q17MR7   A0A182Y9F9   A0A1A9XJ53   A0A2P8ZL88   A0A084WIN4   A0A1I8NHX9   A0A0T6AXC8   A0A067R5V9   A0A0N0BEQ0   W5JWS8   A0A182IX61   A0A2M4CLJ7   A0A182RS07   A0A1B6E4R5   A0A182PYF3   A0A182M3B7   A0A182N2V9   A0A2R7WGB7   A0A336MWL3   E2B3I8   A0A2M4BHG6   A0A2M4BH89   W8BCH1   Q7PZG8   A0A182L530   A0A1S4H7U0   K7J2T9   A0A182XJT8   A0A182UZ32   A0A336M1J7   A0A182TKL7   A0A182QSX5   A0A0L0C1L5   Q9W232   A0A1W4VQN5   Q290H5   N6TXH5   F6ZD81   Q8T9A3   A0A0L7RKL6   A0A088A8Y1   A0A2A3E5W1   B4GC19   B4I868   U4U8C2   B4QHJ3   Q07G10   B4P5H7   A0A096N913   A0A2K5Y7X6   A0A096NCH5   A0A1S3W968   A0A0M5J527   B3NLI7   A0A1S2ZKC4   G1T292   A0A154PK51   K9KC91   F7DYZ5   A0A2I2US18   E2AE04   A0A182KIK7   A0A182WDY8   A0A3B0J0S8   A0A232EJC4   A0A1I8PW84   A0A337S599   A0A3Q7TMR0   E2REE7   A0A3Q7SL51   G3WMU7   A0A182HHJ4   T1J3X4   A0A384C0E5   A0A2Y9DXP8   A0A3Q7WJ91   A0A452TTN1   B3MEC7   E9IFM0   A0A3Q7X508   A0A3Q7XGG4   A0A2K5DGZ9   U3B9R7  

PDB

3THT     E-value=6.67637e-49,     Score=491

KEGG

101736667    K10770 alkylated DNA repair protein alkB homolog 8 [EC:1.14.11.- 2.1.1.229] | (RefSeq) alkylated DNA

GO

GO:0016491   GO:0016300   GO:0002098   GO:0003676   GO:0003723   GO:0000049   GO:0016706   GO:0005634   GO:0005737   GO:0016604   GO:0005829   GO:0005506   GO:0006974   GO:0008270   GO:0030488   GO:0055114   GO:0008152   GO:0008168   GO:0030130   GO:0030132   GO:0015914   GO:0046872   GO:0006412   GO:0016876   GO:0043039  

PANTHER

PTHR13069:SF28  

Subcellular location

Cytoplasm   Predominantly cytoplasmic.   With evidence from 2 publications.
Nucleus   Predominantly cytoplasmic.   With evidence from 2 publications.

Length:

594

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.00479999999999999

Exp number, first 60 AAs:

0.00342

Total prob of N-in:

0.00622

outside

1  -  594

 

 

Pi

14.139163

Theta

14.992233

Tajima's D

-0.434835

CLR

0.338162

CSRT

0.260786960651967

Interpretation

Uncertain