Gene

KWMTBOMO08067  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA001115

Annotation

PREDICTED:_dolichyl-phosphate_beta-glucosyltransferase_[Papilio_polytes]

Location

Bomo_Chr13(-):13900915-13904134      View in EpiBrowser

Full name

Dolichyl-phosphate beta-glucosyltransferase      

Alternative Name

Wollknaeuel

Location in the cell

PlasmaMembrane   Reliability : 3.672

CDS

ATGGATCTCTTAACAATATTTCTGGTAATGGTTTTATATGGTATTGCTATAGCGTTGGCTCTATTAATTGTGCTCGTTATAATACTATATATAATAACCGATACGTATCCTGTTGTGGAGCGGTATAAAGATGAAGAAACATACAATGATTACTTAACAAATACTAAACTGAGATTCCCCTCTATACTTGAAGAAGAATCTGTTAGCTTAAGTGTAGTTGTTCCTGCATATAATGAAGAAAAAAGACTGCCACCCATGTTGGATGAAACAATTGAGTTCTTGGAGAACCGACAAAAAGAAAACCCTTCATACAAATATGAAATAATCATAGTGAGTGACGGAAGTAAAGACAGCACAGTTAAAGTAGCCGAGAGCTACTCCATAAAATATGGTAGTGACAAAGTGAAATGCCTAGAGTTAATAAAGAACAGAGGAAAAGGAGGAGCAGTCAGACTGGGCATACAAAGTTCGAGAGGTGCAACGATATTGTTTGCAGATGCAGATGGTGCCTCAAAATTTGAAGATTTGACGAAATTAGAAGTCGCCCTAAAGGATATTGTGAAATGTGATCCCCTCAAAGATGTTAAAACAACTAGTGAAAGTCTAGGAATTGTAATTGGTTCGAGGGCTCATCTTGAAAAGGAATCTTTAGCAAAAAGGAATATTTTTAGGAATATACTTATGTATGGATTTCATTTTCTGGTCTGGTTGTTCACTGTAAAAGGTATAAAAGACACACAGTGTGGCTTCAAACTTTTCACTAGAAAAGCAGCAAGAATATGTTTTGAAAGCTTACATGTAAACAGATGGGCATTTGATGTAGAGTTATTGTATATCGCACAAAAATTAAACATTCCAATATCCGAAATACCAGTGAGGTGGACAGAAATCGAAGGATCAAAAGTCACGCCAGTTGTCGCTTGGATACAAATGGGATGTGACCTAGGCCTTATCTGGCTTAAATATAGAATAGGAGCTTGGAAGATAAAAAGCGAAAAAACAGAGTGA

Protein

MDLLTIFLVMVLYGIAIALALLIVLVIILYIITDTYPVVERYKDEETYNDYLTNTKLRFPSILEEESVSLSVVVPAYNEEKRLPPMLDETIEFLENRQKENPSYKYEIIIVSDGSKDSTVKVAESYSIKYGSDKVKCLELIKNRGKGGAVRLGIQSSRGATILFADADGASKFEDLTKLEVALKDIVKCDPLKDVKTTSESLGIVIGSRAHLEKESLAKRNIFRNILMYGFHFLVWLFTVKGIKDTQCGFKLFTRKAARICFESLHVNRWAFDVELLYIAQKLNIPISEIPVRWTEIEGSKVTPVVAWIQMGCDLGLIWLKYRIGAWKIKSEKTE

Description

Required for normal production of N-glycosylated proteins in the endoplasmic reticulum (ER). Required for embryonic segmentation, dorsal-ventral patterning and gastrulation. Required for chitin orientation and shaping of the apical and lateral plasma membranes of epidermal cells during cuticle differentiation. Also required for correctly shaping apical membrane topology of the epithelia of other organs such as the midgut and the hindgut.

Catalytic Activity

dolichyl phosphate + UDP-alpha-D-glucose = dolichyl beta-D-glucosyl phosphate + UDP

Miscellaneous

'Wollknaeuel' means 'ball of wool' in German.

Similarity

Belongs to the glycosyltransferase 2 family.

Keywords

Complete proteome   Endoplasmic reticulum   Glycosyltransferase   Membrane   Reference proteome   Signal-anchor   Transferase   Transmembrane   Transmembrane helix  

Feature

chain  Dolichyl-phosphate beta-glucosyltransferase

Uniprot

H9IV35   I4DJP6   A0A194Q6L7   A0A2A4JCR7   A0A2W1BXM9   A0A3S2LRI2   + More
A0A212ERS4   S4PWG3   A0A2H1W5E8   J3JX27   R4WPG9   D2A2D5   A0A1B6E2H3   A0A1W4WHF5   N6TX99   E0VH07   A0A1B6MTM3   A0A224XTH8   A0A067R8A0   A0A146MBZ4   A0A0A9WIG3   A0A2J7R8T5   A0A2P2HW56   A0A2R7VZ92   Q1HQV4   T1HHV7   A0A226E7B7   A0A1W4V7F2   B4Q6C9   B3N733   Q9VLQ1   B3MPG9   B4HYH8   B4NWV4   A0A023EPS4   A0A1Q3FAZ5   E9IJH2   A0A0P5LD01   A0A1Y1K4B3   A0A0P5JZP3   A0A2R5LKZ9   A0A0P5YGC1   A0A0C9RMI0   A0A0P6DYT1   A0A151J999   A0A0P5ZV63   A0A151IM96   A0A0P6EFT9   A0A293MSJ0   A0A131XEG2   A0A224YXL7   A0A131Z2U5   Q29MM8   A0A0P5VAT3   B4G906   A0A158NBT3   F4X8A4   A0A1A9Y3K1   A0A1B0BLA3   L7MIJ2   A0A1Z5KYY3   A0A3P9DFF1   A0A182Y3D5   A0A3B0JBS7   B4KJM4   A0A026W2S2   A0A3Q2VD11   A0A3B4G6E0   A0A0P5TBW9   A0A084WBV6   A0A3P8NID8   A0A195FDB8   M4A2D9   A0A3Q2WIJ1   A0A182SY43   A0A182JYM0   A0A151X9I1   A0A3P9DFD2   A0A3P9P5N1   A0A0P5TLA1   B4LSH2   A0A3B4UM81   A0A3B4YP45   A0A3Q4HIH6   A0A3P8NII2   A0A1B0AEN9   D3TQ93   A0A2U9B7V8   B4MTJ6   A0A3Q2Q6G8   E9FRI2   A0A0P5Q8P3   A0A195BG85   A0A1A9WWC1   A0A0E9X8S6   A0A315VIV5   A0A232F742   I3K425   A0A3B3UVA6   A0A154NXS1  

EC Number

2.4.1.117

Pubmed

19121390    22651552    26354079    28756777    22118469    23622113    + More
22516182    23691247    18362917    19820115    23537049    20566863    24845553    26823975    25401762    17204158    17510324    17994087    22936249    10731132    12537572    12537569    18403407    21199819    17550304    24945155    21282665    28004739    28049606    28797301    26830274    15632085    21347285    21719571    25576852    28528879    25186727    25244985    24508170    30249741    24438588    23542700    20353571    21292972    25613341    29703783    28648823   

EMBL

BABH01000380    AK401514    BAM18136.1    KQ459439    KPJ01019.1    NWSH01001864    + More
PCG69875.1    KZ149891    PZC79081.1    RSAL01000023    RVE52274.1    AGBW02012947    OWR44187.1    GAIX01006838    JAA85722.1    ODYU01006452    SOQ48310.1    BT127795    AEE62757.1    AK417551    BAN20766.1    KQ971338    EFA02177.1    GEDC01022662    GEDC01005172    JAS14636.1    JAS32126.1    APGK01010787    KB738301    KB632179    ENN82673.1    ERL89607.1    DS235156    EEB12663.1    GEBQ01000732    JAT39245.1    GFTR01005147    JAW11279.1    KK852637    KDR19680.1    GDHC01001331    JAQ17298.1    GBHO01038984    GBRD01015182    JAG04620.1    JAG50644.1    NEVH01006721    PNF37240.1    IACF01000178    LAB65982.1    KK854141    PTY12000.1    DQ440340    CH477261    ABF18373.1    EAT45653.1    ACPB03018544    LNIX01000006    OXA53248.1    CM000361    CM002910    EDX04205.1    KMY89030.1    CH954177    EDV59329.1    AE014134    AY094692    AAF52633.1    AAM11045.1    CH902620    EDV31265.1    CH480818    EDW52108.1    CM000157    EDW88484.1    GAPW01002555    JAC11043.1    GFDL01010304    JAV24741.1    GL763802    EFZ19267.1    GDIQ01178928    GDIP01090166    GDIP01087718    GDIP01011833    GDIQ01084258    GDIQ01064775    JAK72797.1    JAM13549.1    GEZM01095847    JAV55298.1    GDIQ01191868    JAK59857.1    GGLE01006046    MBY10172.1    GDIP01168455    GDIP01164650    GDIQ01228113    GDIQ01223565    GDIQ01125904    GDIQ01091147    GDIP01090165    GDIP01088817    GDIP01083764    GDIP01072064    JAK23612.1    JAM31651.1    GBYB01008186    GBYB01008190    JAG77953.1    JAG77957.1    GDIQ01072069    JAN22668.1    KQ979433    KYN21591.1    GDIP01232590    GDIQ01145446    GDIP01137409    GDIP01098462    GDIP01073313    GDIP01039009    JAL06280.1    JAM64706.1    KQ977059    KYN06012.1    GDIP01232589    GDIP01192300    GDIP01137408    GDIP01123151    GDIP01098463    GDIP01073314    GDIQ01077562    JAI90812.1    JAN17175.1    GFWV01019076    MAA43804.1    GEFH01004121    JAP64460.1    GFPF01007374    MAA18520.1    GEDV01002824    JAP85733.1    CH379060    EAL33665.2    GDIP01102955    JAM00760.1    CH479180    EDW28836.1    ADTU01011316    GL888932    EGI57178.1    JXJN01016270    GACK01001003    JAA64031.1    GFJQ02006969    JAW00001.1    OUUW01000004    SPP79804.1    CH933807    EDW11469.1    KK107459    QOIP01000004    EZA50377.1    RLU23708.1    GDIP01128250    JAL75464.1    ATLV01022472    KE525332    KFB47700.1    KQ981673    KYN38376.1    KQ982373    KYQ57023.1    GDIP01128208    JAL75506.1    CH940649    EDW64794.1    EZ423595    ADD19871.1    CP026246    AWO99915.1    AWO99916.1    CH963852    EDW75435.1    GL732523    EFX90194.1    GDIQ01117652    JAL34074.1    KQ976500    KYM83175.1    GBXM01010347    JAH98230.1    NHOQ01001678    PWA22876.1    NNAY01000752    OXU26676.1    AERX01050045    KQ434778    KZC04417.1   

Proteomes

UP000005204    UP000053268    UP000218220    UP000283053    UP000007151    UP000007266    + More
UP000192223    UP000019118    UP000030742    UP000009046    UP000027135    UP000235965    UP000008820    UP000015103    UP000198287    UP000192221    UP000000304    UP000008711    UP000000803    UP000007801    UP000001292    UP000002282    UP000078492    UP000078542    UP000001819    UP000008744    UP000005205    UP000007755    UP000092443    UP000092460    UP000265160    UP000076408    UP000268350    UP000009192    UP000053097    UP000279307    UP000264840    UP000261460    UP000030765    UP000265100    UP000078541    UP000002852    UP000075901    UP000075881    UP000075809    UP000242638    UP000008792    UP000261420    UP000261360    UP000261580    UP000092445    UP000246464    UP000007798    UP000265000    UP000000305    UP000078540    UP000091820    UP000215335    UP000005207    UP000261500    UP000076502   

PRIDE

H9IV35    Q9VLQ1   

Pfam

PF00535   Glycos_transf_2        + More
PF00431   CUB
PF07645   EGF_CA
PF00008   EGF

Interpro

IPR029044   Nucleotide-diphossugar_trans        + More
IPR035518   DPG_synthase       
IPR001173   Glyco_trans_2-like       
IPR009030   Growth_fac_rcpt_cys_sf       
IPR018097   EGF_Ca-bd_CS       
IPR013032   EGF-like_CS       
IPR000152   EGF-type_Asp/Asn_hydroxyl_site       
IPR001881   EGF-like_Ca-bd_dom       
IPR000742   EGF-like_dom       
IPR000859   CUB_dom       
IPR035914   Sperma_CUB_dom_sf       

SUPFAM

SSF53448   SSF53448        + More
SSF49854   SSF49854       
SSF57184   SSF57184       

Gene 3D

3.90.550.10     2.60.120.290    

CDD

cd04188  DPG_synthase
cd00041  CUB

ProteinModelPortal

H9IV35   I4DJP6   A0A194Q6L7   A0A2A4JCR7   A0A2W1BXM9   A0A3S2LRI2   + More
A0A212ERS4   S4PWG3   A0A2H1W5E8   J3JX27   R4WPG9   D2A2D5   A0A1B6E2H3   A0A1W4WHF5   N6TX99   E0VH07   A0A1B6MTM3   A0A224XTH8   A0A067R8A0   A0A146MBZ4   A0A0A9WIG3   A0A2J7R8T5   A0A2P2HW56   A0A2R7VZ92   Q1HQV4   T1HHV7   A0A226E7B7   A0A1W4V7F2   B4Q6C9   B3N733   Q9VLQ1   B3MPG9   B4HYH8   B4NWV4   A0A023EPS4   A0A1Q3FAZ5   E9IJH2   A0A0P5LD01   A0A1Y1K4B3   A0A0P5JZP3   A0A2R5LKZ9   A0A0P5YGC1   A0A0C9RMI0   A0A0P6DYT1   A0A151J999   A0A0P5ZV63   A0A151IM96   A0A0P6EFT9   A0A293MSJ0   A0A131XEG2   A0A224YXL7   A0A131Z2U5   Q29MM8   A0A0P5VAT3   B4G906   A0A158NBT3   F4X8A4   A0A1A9Y3K1   A0A1B0BLA3   L7MIJ2   A0A1Z5KYY3   A0A3P9DFF1   A0A182Y3D5   A0A3B0JBS7   B4KJM4   A0A026W2S2   A0A3Q2VD11   A0A3B4G6E0   A0A0P5TBW9   A0A084WBV6   A0A3P8NID8   A0A195FDB8   M4A2D9   A0A3Q2WIJ1   A0A182SY43   A0A182JYM0   A0A151X9I1   A0A3P9DFD2   A0A3P9P5N1   A0A0P5TLA1   B4LSH2   A0A3B4UM81   A0A3B4YP45   A0A3Q4HIH6   A0A3P8NII2   A0A1B0AEN9   D3TQ93   A0A2U9B7V8   B4MTJ6   A0A3Q2Q6G8   E9FRI2   A0A0P5Q8P3   A0A195BG85   A0A1A9WWC1   A0A0E9X8S6   A0A315VIV5   A0A232F742   I3K425   A0A3B3UVA6   A0A154NXS1  

PDB

5EKE     E-value=6.48646e-05,     Score=109

KEGG

101738398    K00729 dolichyl-phosphate beta-glucosyltransferase [EC:2.4.1.117] | (RefSeq) dolichyl-phosphate beta

PATHWAY

00510    N-Glycan biosynthesis - Bombyx mori (domestic silkworm)
01100    Metabolic pathways - Bombyx mori (domestic silkworm)

GO

GO:0016021   GO:0016740   GO:0005509   GO:0016757   GO:0006487   GO:0005789   GO:0004581   GO:0009953   GO:0007029   GO:0007030   GO:0006493   GO:0007509   GO:0007009   GO:0005783   GO:0040003   GO:0019991   GO:0010004   GO:0007379  

Subcellular location

Endoplasmic reticulum membrane  

Length:

335

Number of predicted TMHs:

2

Exp number of AAs in TMHs:

44.7803

Exp number, first 60 AAs:

23.42425

Total prob of N-in:

0.05651

POSSIBLE N-term signal

sequence

outside

1  -  9

TMhelix

10  -  32

inside

33  -  220

TMhelix

221  -  240

outside

241  -  335

 

 

Pi

21.772285

Theta

19.914223

Tajima's D

0.256311

CLR

4.510425

CSRT

0.455927203639818

Interpretation

Uncertain