Gene

KWMTBOMO08061

Pre Gene Modal

BGIBMGA001118

Annotation

PREDICTED:_7-methylguanosine_phosphate-specific_5'-nucleotidase_[Papilio_machaon]

Location

Bomo_Chr13(-):13866516-13866954      View in EpiBrowser

Full name

5'-nucleotidase       + More
7-methylguanosine phosphate-specific 5'-nucleotidase      

Alternative Name

Cytosolic 5'-nucleotidase IIIB
N(7)-methylguanylate 5'-phosphatase

Location in the cell

Cytoplasmic   Reliability : 1.333 Mitochondrial   Reliability : 1.309 Nuclear   Reliability : 1.384

CDS

ATGAAGGTTTTAACGAGGCTTCAAGAAATTCCGGAGTTTAACAGAGAAAACATCCATATTAGAGACGAGACGGAACTAGTCGCGAAGTTAAATAGAATCATAAAAGGCGGACACAACAAATTGCAGATAGTGACAGATTTTGACCATACGCTGACAAGACACACTTTGGATAATGGAAAATCCGTTCTCACTAGTTTTGGTATGTGA

Protein

MKVLTRLQEIPEFNRENIHIRDETELVAKLNRIIKGGHNKLQIVTDFDHTLTRHTLDNGKSVLTSFGM

Description

Specifically hydrolyzes 7-methylguanosine monophosphate (m(7)GMP) to 7-methylguanosine and inorganic phosphate (PubMed:23223233, PubMed:24603684). Also able to mediate hydrolysis of diphosphate (m(7)GDP) to 7-methylguanosine and 2 inorganic phosphate with lower activity (PubMed:23223233). The specific activity for m(7)GMP may protect cells against undesired salvage of m(7)GMP and its incorporation into nucleic acids (PubMed:23223233). Also has weak activity for CMP (PubMed:23223233, PubMed:24603684). UMP and purine nucleotides are poor substrates (PubMed:23223233, PubMed:24603684).

Catalytic Activity

a ribonucleoside 5'-phosphate + H2O = a ribonucleoside + phosphate
7-methyl-GMP + H2O = N(7)-methylguanosine + phosphate
CMP + H2O = cytidine + phosphate

Cofactor

Mg(2+)

Biophysicochemical Properties

13 uM for m(7)GMP (at 25 degrees Celsius)
12 uM for m(7)GMP (at 25 degrees Celsius)
48 uM for CMP (at 25 degrees Celsius)
102 uM for GMP (at 25 degrees Celsius)
32 uM for AMP (at 25 degrees Celsius)
91 uM for UMP (at 25 degrees Celsius)

Subunit

Monomer.

Similarity

Belongs to the pyrimidine 5'-nucleotidase family.

Keywords

3D-structure   Complete proteome   Hydrolase   Magnesium   Metal-binding   Nucleotide metabolism   Nucleotide-binding   Reference proteome  

Feature

chain  7-methylguanosine phosphate-specific 5'-nucleotidase

Uniprot

H9IV38   A0A2A4JCN7   A0A2W1C271   A0A194RDF8   A0A2H1VTT7   A0A3S2PIF6   + More
A0A194Q6L2   S4PX53   A0A1E1WSJ8   A0A0L7KR62   A0A212ERR6   A0A182FVZ2   B0X0T4   A0A182JQ60   A0A1Q3FGY6   A0A2M3ZI08   A0A2M4BW44   A0A0L7LM38   W5JP62   A0A182PN54   A0A336LZD2   A0A182J590   A0A084WUG0   A0A182KUZ0   A0A182UNK3   A0A182WY01   Q7QE29   A0A182I621   A0A0L7KRZ7   A0A2M4ALI7   T1E8J9   A0A182UBB7   A0A182NPA1   A0A182YAB5   A0A182Q2A3   A0A182LXU3   A0A182WE85   A0A182S7E1   A0A182RLV4   A0A1B0DDY6   Q17PQ5   A0A023EQ93   U5EYF0   A0A0M4EH73   B4KM33   A0A026W2Z4   E2A4U6   A0A034WB86   A0A1B0CBQ1   A0A1I8NLH3   A0A0J7KV79   W8BJC5   A0A2G9S4V8   E2C413   A0A0A1WQY6   Q9W197   A0A034W9L4   A0A0K8VG61   A0A195BG84   A0A195FDT2   A0A195DRX9   A0A158NBS5   B4I2F1   B4QBK0   A0A1B6KYL7   A0A1B6H3L6   A0A1B6KZG4   B3MDM8   A0A2P8Z7N4   A0A2G9S4X1   A0A0L0C605   A0A1Y1M1Z2   A0A1B6FFA8   A0A3N0YA27   A0A1A9ZQS9   A0A1A9UER1   A0A1B0FE50   A0A0T6B439   B4PAW2   A0A1W4UM40   A0A151X9L3   D1ZZV3   B4LK41   A0A023F065   A0A1L8DXW8   A0A232FCJ5   K7J061   A0A088ATS9   A0A0P4VK67   A0A2D4IWN5   A0A1L8DXY4   A0A2D4IWS6   A0A1B6KHD2   A0A1B6C974   B4J4S4   A0A1B6MFL6   S4RFV0   F7DEV4   D3TP59   A0A1B0BYQ4  

EC Number

3.1.3.5
3.1.3.91

Pubmed

19121390    28756777    26354079    23622113    26227816    22118469    + More
20920257    23761445    24438588    20966253    12364791    14747013    17210077    25244985    17510324    24945155    17994087    18057021    24508170    30249741    20798317    25348373    24495485    25830018    10731132    12537572    12537569    23223233    24603684    21347285    22936249    29403074    26108605    28004739    17550304    18362917    19820115    25474469    28648823    20075255    27129103    17495919    20353571   

EMBL

BABH01000388    NWSH01001864    PCG69867.1    KZ149891    PZC79076.1    KQ460397    + More
KPJ15315.1    ODYU01004396    SOQ44230.1    RSAL01000023    RVE52277.1    KQ459439    KPJ01014.1    GAIX01005638    JAA86922.1    GDQN01010614    GDQN01004724    GDQN01001049    JAT80440.1    JAT86330.1    JAT90005.1    JTDY01006698    KOB65763.1    AGBW02012947    OWR44182.1    DS232245    EDS38326.1    GFDL01008250    JAV26795.1    GGFM01007354    MBW28105.1    GGFJ01007807    MBW56948.1    JTDY01000627    KOB76419.1    ADMH02000766    ETN65108.1    UFQT01000339    SSX23354.1    ATLV01027083    KE525423    KFB53854.1    AAAB01008848    EAA07027.4    APCN01003547    JTDY01006610    KOB65836.1    GGFK01008332    MBW41653.1    GAMD01002708    JAA98882.1    AXCN02001814    AXCM01001000    AJVK01014661    CH477190    EAT48696.1    GAPW01002874    JAC10724.1    GANO01002042    JAB57829.1    CP012524    ALC41872.1    CH933808    EDW10822.1    KK107459    QOIP01000004    EZA50393.1    RLU24234.1    GL436748    EFN71540.1    GAKP01007552    JAC51400.1    AJWK01005748    AJWK01005749    LBMM01002768    KMQ94397.1    GAMC01007708    JAB98847.1    KV925179    PIO35124.1    GL452364    EFN77399.1    GBXI01013035    JAD01257.1    AE013599    AY061358    GAKP01007553    JAC51399.1    GDHF01014400    JAI37914.1    KQ976500    KYM83187.1    KQ981673    KYN38362.1    KQ980581    KYN15269.1    ADTU01011295    CH480820    EDW53946.1    CM000362    CM002911    EDX08518.1    KMY96290.1    GEBQ01023476    JAT16501.1    GECZ01000508    JAS69261.1    GEBQ01023192    JAT16785.1    CH902619    EDV37491.1    PYGN01000160    PSN52497.1    PIO35125.1    JRES01000857    KNC27715.1    GEZM01042433    JAV79854.1    GECZ01029236    GECZ01020891    GECZ01003524    JAS40533.1    JAS48878.1    JAS66245.1    RJVU01048900    ROL43072.1    CCAG010006200    LJIG01009893    KRT82170.1    CM000158    EDW92502.1    KQ982373    KYQ57009.1    KQ971338    EFA01792.1    CH940648    EDW60630.1    GBBI01003815    JAC14897.1    GFDF01002777    JAV11307.1    NNAY01000467    OXU28168.1    GDKW01001904    JAI54691.1    IACK01139303    LAA88665.1    GFDF01002776    JAV11308.1    IACK01139301    LAA88662.1    GEBQ01029413    JAT10564.1    GEDC01027265    GEDC01026000    JAS10033.1    JAS11298.1    CH916367    EDW00620.1    GEBQ01005298    JAT34679.1    EZ423211    ADD19487.1    JXJN01022766   

Proteomes

UP000005204    UP000218220    UP000053240    UP000283053    UP000053268    UP000037510    + More
UP000007151    UP000069272    UP000002320    UP000075881    UP000000673    UP000075885    UP000075880    UP000030765    UP000075882    UP000075903    UP000076407    UP000007062    UP000075840    UP000075902    UP000075884    UP000076408    UP000075886    UP000075883    UP000075920    UP000075901    UP000075900    UP000092462    UP000008820    UP000092553    UP000009192    UP000053097    UP000279307    UP000000311    UP000092461    UP000095300    UP000036403    UP000008237    UP000000803    UP000078540    UP000078541    UP000078492    UP000005205    UP000001292    UP000000304    UP000007801    UP000245037    UP000037069    UP000092445    UP000078200    UP000092444    UP000002282    UP000192221    UP000075809    UP000007266    UP000008792    UP000215335    UP000002358    UP000005203    UP000001070    UP000245300    UP000002280    UP000092460   

PRIDE

H9IV38    Q9W197   

Pfam

PF05822   UMPH-1        + More
PF08424   NRDE-2
PF06094   GGACT
PF00632   HECT

Interpro

IPR036412   HAD-like_sf        + More
IPR023214   HAD_sf       
IPR006434   Pyrimidine_nucleotidase_eu       
IPR009288   AIG2-like       
IPR013633   NRDE-2       
IPR013024   GGCT-like       
IPR036568   GGCT-like_sf       
IPR008265   Lipase_GDSL_AS       
IPR000048   IQ_motif_EF-hand-BS       
IPR000569   HECT_dom       
IPR035983   Hect_E3_ubiquitin_ligase       
IPR013087   Znf_C2H2_type       

SUPFAM

SSF56784   SSF56784        + More
SSF110857   SSF110857       
SSF56204   SSF56204       

Gene 3D

3.40.50.1000    

CDD

cd06661  GGCT_like
cd07504  HAD_5NT
cd00078  HECTc

ProteinModelPortal

H9IV38   A0A2A4JCN7   A0A2W1C271   A0A194RDF8   A0A2H1VTT7   A0A3S2PIF6   + More
A0A194Q6L2   S4PX53   A0A1E1WSJ8   A0A0L7KR62   A0A212ERR6   A0A182FVZ2   B0X0T4   A0A182JQ60   A0A1Q3FGY6   A0A2M3ZI08   A0A2M4BW44   A0A0L7LM38   W5JP62   A0A182PN54   A0A336LZD2   A0A182J590   A0A084WUG0   A0A182KUZ0   A0A182UNK3   A0A182WY01   Q7QE29   A0A182I621   A0A0L7KRZ7   A0A2M4ALI7   T1E8J9   A0A182UBB7   A0A182NPA1   A0A182YAB5   A0A182Q2A3   A0A182LXU3   A0A182WE85   A0A182S7E1   A0A182RLV4   A0A1B0DDY6   Q17PQ5   A0A023EQ93   U5EYF0   A0A0M4EH73   B4KM33   A0A026W2Z4   E2A4U6   A0A034WB86   A0A1B0CBQ1   A0A1I8NLH3   A0A0J7KV79   W8BJC5   A0A2G9S4V8   E2C413   A0A0A1WQY6   Q9W197   A0A034W9L4   A0A0K8VG61   A0A195BG84   A0A195FDT2   A0A195DRX9   A0A158NBS5   B4I2F1   B4QBK0   A0A1B6KYL7   A0A1B6H3L6   A0A1B6KZG4   B3MDM8   A0A2P8Z7N4   A0A2G9S4X1   A0A0L0C605   A0A1Y1M1Z2   A0A1B6FFA8   A0A3N0YA27   A0A1A9ZQS9   A0A1A9UER1   A0A1B0FE50   A0A0T6B439   B4PAW2   A0A1W4UM40   A0A151X9L3   D1ZZV3   B4LK41   A0A023F065   A0A1L8DXW8   A0A232FCJ5   K7J061   A0A088ATS9   A0A0P4VK67   A0A2D4IWN5   A0A1L8DXY4   A0A2D4IWS6   A0A1B6KHD2   A0A1B6C974   B4J4S4   A0A1B6MFL6   S4RFV0   F7DEV4   D3TP59   A0A1B0BYQ4  

PDB

4NWI     E-value=1.64802e-08,     Score=134

KEGG

101738667    K01081 5'-nucleotidase [EC:3.1.3.5] | (RefSeq) 7-methylguanosine phosphate-specific 5'-nucleotidase

PATHWAY

00230    Purine metabolism - Bombyx mori (domestic silkworm)
00240    Pyrimidine metabolism - Bombyx mori (domestic silkworm)
00760    Nicotinate and nicotinamide metabolism - Bombyx mori (domestic silkworm)
01100    Metabolic pathways - Bombyx mori (domestic silkworm)

GO

GO:0005737   GO:0008253   GO:0000287   GO:0009117   GO:0000166   GO:0016021   GO:0006629   GO:0016298   GO:0005829   GO:0009158   GO:1901069   GO:0008252   GO:0016311   GO:0004842  

PANTHER

PTHR13471  

Subcellular location

Cytoplasm  

Length:

68

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0

Exp number, first 60 AAs:

0

Total prob of N-in:

0.38775

outside

1  -  68

 

 

Pi

11.601708

Theta

9.146509

Tajima's D

0.783046

CLR

0.221912

CSRT

0.613319334033298

Interpretation

Uncertain