SGID Silkworm Genome Informatics Database
Gene
KWMTBOMO07940
Pre Gene Modal
BGIBMGA001163
Annotation
PREDICTED:_cAMP-dependent_protein_kinase_catalytic_subunit_alpha-like_[Papilio_xuthus]
Full name
cAMP-dependent protein kinase catalytic subunit       + More
cAMP-dependent protein kinase catalytic subunit alpha      
Location in the cell
Cytoplasmic   Reliability : 3.429
 

Sequence

CDS
ATGGCAGATAGTGAAATTAAAAGGCATATAAATTCACCACGTGGTGATTTGATACAACACGGTGGCTATTCTCCTGCAAAACAAGAACAATACTCTCAATATTTAAAGAAACTCCGTCAAGATTTTGTTGAGTCATGGGATGATAAATCTGAAAGTAATGCAAAGTTGAATGATTTTGAAAGACAAAAAGTTTTAGGAACAGGAGCATTTGGCATTGTTTTTCTTGTAAAGCATATTGTAACAGGTAAATATTATGCTATGAAAATGCTCGAAAAAGAGAAAATTGTTAGATTGAAACAAATTGAGCACAGCTACTACGAAAAAAAGATTCTATGTGGCTTAAATTTTCCTTTTGTTGTTTACATGAAATACTATTTTAAGGATAACGTTTATCTTTATTACGTTCTACCTTTCATAGCCGGTGGGGAAATGTTTTCACATTTACGTAAAATGGGCAAGTTTGAAGAAACTTCTGCAAAATTTTACGGAGCACAAGTTATACTAGCATTGGAATATCTGCACGCTTGTGAATTAGTATATCGAGATCTAAAGCCCGAGAATATATTAATAGATCGTACAGGTTATATCAAAATTACTGACTTCGGTTTCTGTAAGCTTATCCGCGGTAGGACTTGGACACTTTGCGGTACTCCAGAATATTTAGCACCTGAAATCATTCTAAGCAAAGGCTATGGAATGTCAGTCGACTGGTGGTCCCTAGGGGTATTACTTTTCGAAATGAGTGCCGGGCATCCACCATTTTTTGCTTCGGATCCAATGAGAATTTATGAAAAAATTGTGGCAGGAAAATATAGATGTCCATCACATTTTTCTGTTGAATTAAAAGATATAATAGGACATGTTTTACAAGTCGACACAACAAGACGTTACGGAACTCTTAAAGATGGCGTGTTGGATTTTAAAAATCATAAATGGTTTAGAGAAATCGATTGGGATAGTATTTTGAATAGTCGCATACATCCGCCGTTTTTTCCAAAGATACGTTCACCAGGCGACACCACTAATTTCGATGTTTTTGATGAAGAAAAAATTAAAGACAGTCCAGTATGTCTTTATGAAGAAGAATTTGCTGATTTTTAA
Protein
MADSEIKRHINSPRGDLIQHGGYSPAKQEQYSQYLKKLRQDFVESWDDKSESNAKLNDFERQKVLGTGAFGIVFLVKHIVTGKYYAMKMLEKEKIVRLKQIEHSYYEKKILCGLNFPFVVYMKYYFKDNVYLYYVLPFIAGGEMFSHLRKMGKFEETSAKFYGAQVILALEYLHACELVYRDLKPENILIDRTGYIKITDFGFCKLIRGRTWTLCGTPEYLAPEIILSKGYGMSVDWWSLGVLLFEMSAGHPPFFASDPMRIYEKIVAGKYRCPSHFSVELKDIIGHVLQVDTTRRYGTLKDGVLDFKNHKWFREIDWDSILNSRIHPPFFPKIRSPGDTTNFDVFDEEKIKDSPVCLYEEEFADF

Summary

Description
Essential for larval development (PubMed:22887816). Controls the rhythmic contraction of enteric muscles probably by regulating G-protein coupled receptor aex-2-mediated calcium influx in GABAergic DVB neurons (PubMed:24086161). Plays a role in the control of oocyte meiotic maturation by gonadal sheath cells (PubMed:22887816).
Isoforms a and b: May play a role in the regulation of neuromuscular junctions.
Phosphorylates a large number of substrates in the cytoplasm and the nucleus. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. Phosphorylates CDC25B, ABL1, NFKB1, CLDN3, PSMC5/RPT6, PJA2, RYR2, RORA and VASP. RORA is activated by phosphorylation. Required for glucose-mediated adipogenic differentiation increase and osteogenic differentiation inhibition from osteoblasts. Involved in the regulation of platelets in response to thrombin and collagen; maintains circulating platelets in a resting state by phosphorylating proteins in numerous platelet inhibitory pathways when in complex with NF-kappa-B (NFKB1 and NFKB2) and I-kappa-B-alpha (NFKBIA), but thrombin and collagen disrupt these complexes and free active PRKACA stimulates platelets and leads to platelet aggregation by phosphorylating VASP. Prevents the antiproliferative and anti-invasive effects of alpha-difluoromethylornithine in breast cancer cells when activated. RYR2 channel activity is potentiated by phosphorylation in presence of luminal Ca(2+), leading to reduced amplitude and increased frequency of store overload-induced Ca(2+) release (SOICR) characterized by an increased rate of Ca(2+) release and propagation velocity of spontaneous Ca(2+) waves, despite reduced wave amplitude and resting cytosolic Ca(2+). PSMC5/RPT6 activation by phosphorylation stimulates proteasome. Negatively regulates tight junctions (TJs) in ovarian cancer cells via CLDN3 phosphorylation. NFKB1 phosphorylation promotes NF-kappa-B p50-p50 DNA binding. Involved in embryonic development by down-regulating the Hedgehog (Hh) signaling pathway that determines embryo pattern formation and morphogenesis. Prevents meiosis resumption in prophase-arrested oocytes via CDC25B inactivation by phosphorylation. May also regulate rapid eye movement (REM) sleep in the pedunculopontine tegmental (PPT) (By similarity). Phosphorylates APOBEC3G and AICDA (By similarity). Phosphorylates HSF1; this phosphorylation promotes HSF1 nuclear localization and transcriptional activity upon heat shock (By similarity).
Catalytic Activity
ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein]
ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein]
Subunit
Heterotetramer composed of two regulatory subunits and two catalytic subunits.
A number of inactive tetrameric holoenzymes are produced by the combination of homo- or heterodimers of the different regulatory subunits associated with two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Activates cAMP-sensitive PKAI and PKAII holoenzymes by interacting with regulatory subunit (R) of PKA, PRKAR1A/PKR1 and PRKAR2A/PKR2, respectively. Interacts with NFKB1, NFKB2 and NFKBIA in platelets; these interactions are disrupted by thrombin and collagen. Binds to ABL1 in spermatozoa and with CDC25B in oocytes (By similarity). Interacts with APOBEC3G and AICDA (By similarity). Interacts with RAB13; downstream effector of RAB13 involved in tight junction assembly. Found in a complex at least composed of MROH2B, PRKACA and TCP11 (By similarity). Interacts with MROH2B (By similarity). Interacts with TCP11 (By similarity). Interacts with HSF1 (By similarity).
Similarity
Belongs to the protein kinase superfamily.
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily.
Keywords
Alternative splicing   ATP-binding   cAMP   Complete proteome   Kinase   Lipoprotein   Myristate   Nucleotide-binding   Phosphoprotein   Reference proteome   Transferase   3D-structure   Cell membrane   Cell projection   Cilium   Cytoplasm   Cytoplasmic vesicle   Direct protein sequencing   Flagellum   Membrane   Mitochondrion   Nucleus  
Feature
chain  cAMP-dependent protein kinase catalytic subunit
splice variant  In isoform c.
EC Number
2.7.11.11
EMBL
BABH01000681    KQ459299    KPJ01909.1    KQ460202    KPJ17176.1    JTDY01001711    + More
KOB73103.1    AGBW02013095    OWR43992.1    AGBW02012010    OWR45805.1    JTDY01000340    KOB77630.1    KQ460855    KPJ11867.1    KQ459584    KPI98576.1    AAZO01001585    DS235093    EEB11858.1    GBYB01011506    JAG81273.1    KQ414637    KOC66936.1    AADN05000647    MUZQ01000002    OWK64503.1    LIAE01010642    PAV57368.1    GEZM01015981    JAV91396.1    AGTO01021089    KL262375    KFZ60277.1    AKCR02000018    PKK28038.1    KK408331    KFQ83376.1    KK945962    KFQ54356.1    LMAW01003072    KQK74224.1    KL217788    KFO98612.1    AKHW03002907    KYO36790.1    KK734081    KFR03712.1    AFYH01051841    AFYH01051842    AFYH01051843    AFYH01051844    AFYH01051845    AFYH01051846    AFYH01051847    AFYH01051848    AFYH01051849    AFYH01051850    JW867900    AFP00418.1    GBSH01000536    JAG68489.1    GDAY02000383    JAV51030.1    JU174069    GBEX01000552    AFJ49595.1    JAI14008.1    GAAZ01000411    GBKC01000571    JAA97532.1    JAG45499.1    GAEP01000269    GBEW01000184    JAB54552.1    JAI10181.1    KL350738    KFZ58739.1    KYO36791.1    KL485232    KFO09386.1    KL380329    KFP86838.1    PAV57358.1    KL248304    KFV16254.1    LSYS01005497    OPJ77408.1    KL520265    KFO89395.1    QUSF01000010    RLW06015.1    KYO36792.1    KK540411    KFP30707.1    KL447384    KFO72782.1    M37119    M37114    M37115    M37116    M37117    M37118    Z82096    Z81511    AJ011936    AJ011937    AJ011938    AJ012354    AJ012355    AJ012356    KK718445    KFO56612.1    KL224623    KFW62215.1    KL225597    KFM00721.1    KL410872    KFR00341.1    KK527473    KFP68664.1    KL300969    KFP51276.1    KL871737    KGL93061.1    AWGT02000057    OXB80050.1    PKK28039.1    AGCU01066600    AGCU01066601    AGCU01066602    AGCU01066603    AGCU01066604    AGCU01066605    AGCU01066606    AGCU01066607    AGCU01066608    AGCU01066609    KB743342    EOA99300.1    ABQF01041419    ABQF01041420    ABQF01041421    ABQF01041422    ABQF01041423    PAV57363.1    PAV57369.1    KK802033    KFQ29452.1    KK692017    KFQ22039.1    KL206233    KFV80346.1    KL215901    KFV67258.1    KL670376    KFW78932.1    KK501110    KFP12867.1    GBSI01000439    JAC96057.1    KK678206    KFQ10696.1    AAWZ02004438    QOIP01000003    RLU25256.1    JW871135    AFP03653.1    AAMC01003225    AAMC01003226    AAMC01003227    AAMC01003228    AAMC01003229    AAMC01003230    KU193794    ANB82449.1    AQIA01003548    AQIA01003549    AQIA01003550    AQIA01003551    JU335387    JV043888    AFE79140.1    AFI33959.1    X67154   
Pfam
PF00069   Pkinase
Interpro
IPR000719   Prot_kinase_dom        + More
IPR000961   AGC-kinase_C       
IPR017441   Protein_kinase_ATP_BS       
IPR011009   Kinase-like_dom_sf       
IPR008271   Ser/Thr_kinase_AS       
IPR039083   PKA_C-beta       
IPR039035   PKA_C-alpha       
SUPFAM
SSF56112   SSF56112       
PDB
1YDT     E-value=1.19194e-124,     Score=1142

Ontologies

Topology

Subcellular location
Cytoplasm   Translocates into the nucleus (monomeric catalytic subunit). The inactive holoenzyme is found in the cytoplasm. Distributed throughout the cytoplasm in meiotically incompetent oocytes. Associated to mitochondrion as meiotic competence is acquired. Aggregates around the germinal vesicles (GV) at the immature GV stage oocytes (By similarity). Colocalizes with MROH2B and TCP11 on the acrosome and tail regions in round spermatids and spermatozoa regardless of the capacitation status of the sperm (By similarity). Colocalizes with HSF1 in nuclear stress bodies (nSBs) upon heat shock (By similarity).   With evidence from 9 publications.
Nucleus   Translocates into the nucleus (monomeric catalytic subunit). The inactive holoenzyme is found in the cytoplasm. Distributed throughout the cytoplasm in meiotically incompetent oocytes. Associated to mitochondrion as meiotic competence is acquired. Aggregates around the germinal vesicles (GV) at the immature GV stage oocytes (By similarity). Colocalizes with MROH2B and TCP11 on the acrosome and tail regions in round spermatids and spermatozoa regardless of the capacitation status of the sperm (By similarity). Colocalizes with HSF1 in nuclear stress bodies (nSBs) upon heat shock (By similarity).   With evidence from 9 publications.
Cell membrane   Translocates into the nucleus (monomeric catalytic subunit). The inactive holoenzyme is found in the cytoplasm. Distributed throughout the cytoplasm in meiotically incompetent oocytes. Associated to mitochondrion as meiotic competence is acquired. Aggregates around the germinal vesicles (GV) at the immature GV stage oocytes (By similarity). Colocalizes with MROH2B and TCP11 on the acrosome and tail regions in round spermatids and spermatozoa regardless of the capacitation status of the sperm (By similarity). Colocalizes with HSF1 in nuclear stress bodies (nSBs) upon heat shock (By similarity).   With evidence from 9 publications.
Mitochondrion   Translocates into the nucleus (monomeric catalytic subunit). The inactive holoenzyme is found in the cytoplasm. Distributed throughout the cytoplasm in meiotically incompetent oocytes. Associated to mitochondrion as meiotic competence is acquired. Aggregates around the germinal vesicles (GV) at the immature GV stage oocytes (By similarity). Colocalizes with MROH2B and TCP11 on the acrosome and tail regions in round spermatids and spermatozoa regardless of the capacitation status of the sperm (By similarity). Colocalizes with HSF1 in nuclear stress bodies (nSBs) upon heat shock (By similarity).   With evidence from 9 publications.
Membrane   Translocates into the nucleus (monomeric catalytic subunit). The inactive holoenzyme is found in the cytoplasm. Distributed throughout the cytoplasm in meiotically incompetent oocytes. Associated to mitochondrion as meiotic competence is acquired. Aggregates around the germinal vesicles (GV) at the immature GV stage oocytes (By similarity). Colocalizes with MROH2B and TCP11 on the acrosome and tail regions in round spermatids and spermatozoa regardless of the capacitation status of the sperm (By similarity). Colocalizes with HSF1 in nuclear stress bodies (nSBs) upon heat shock (By similarity).   With evidence from 9 publications.
Cell projection   Translocates into the nucleus (monomeric catalytic subunit). The inactive holoenzyme is found in the cytoplasm. Distributed throughout the cytoplasm in meiotically incompetent oocytes. Associated to mitochondrion as meiotic competence is acquired. Aggregates around the germinal vesicles (GV) at the immature GV stage oocytes (By similarity). Colocalizes with MROH2B and TCP11 on the acrosome and tail regions in round spermatids and spermatozoa regardless of the capacitation status of the sperm (By similarity). Colocalizes with HSF1 in nuclear stress bodies (nSBs) upon heat shock (By similarity).   With evidence from 9 publications.
Cilium   Translocates into the nucleus (monomeric catalytic subunit). The inactive holoenzyme is found in the cytoplasm. Distributed throughout the cytoplasm in meiotically incompetent oocytes. Associated to mitochondrion as meiotic competence is acquired. Aggregates around the germinal vesicles (GV) at the immature GV stage oocytes (By similarity). Colocalizes with MROH2B and TCP11 on the acrosome and tail regions in round spermatids and spermatozoa regardless of the capacitation status of the sperm (By similarity). Colocalizes with HSF1 in nuclear stress bodies (nSBs) upon heat shock (By similarity).   With evidence from 9 publications.
Flagellum   Translocates into the nucleus (monomeric catalytic subunit). The inactive holoenzyme is found in the cytoplasm. Distributed throughout the cytoplasm in meiotically incompetent oocytes. Associated to mitochondrion as meiotic competence is acquired. Aggregates around the germinal vesicles (GV) at the immature GV stage oocytes (By similarity). Colocalizes with MROH2B and TCP11 on the acrosome and tail regions in round spermatids and spermatozoa regardless of the capacitation status of the sperm (By similarity). Colocalizes with HSF1 in nuclear stress bodies (nSBs) upon heat shock (By similarity).   With evidence from 9 publications.
Cytoplasmic vesicle   Translocates into the nucleus (monomeric catalytic subunit). The inactive holoenzyme is found in the cytoplasm. Distributed throughout the cytoplasm in meiotically incompetent oocytes. Associated to mitochondrion as meiotic competence is acquired. Aggregates around the germinal vesicles (GV) at the immature GV stage oocytes (By similarity). Colocalizes with MROH2B and TCP11 on the acrosome and tail regions in round spermatids and spermatozoa regardless of the capacitation status of the sperm (By similarity). Colocalizes with HSF1 in nuclear stress bodies (nSBs) upon heat shock (By similarity).   With evidence from 9 publications.
Secretory vesicle   Translocates into the nucleus (monomeric catalytic subunit). The inactive holoenzyme is found in the cytoplasm. Distributed throughout the cytoplasm in meiotically incompetent oocytes. Associated to mitochondrion as meiotic competence is acquired. Aggregates around the germinal vesicles (GV) at the immature GV stage oocytes (By similarity). Colocalizes with MROH2B and TCP11 on the acrosome and tail regions in round spermatids and spermatozoa regardless of the capacitation status of the sperm (By similarity). Colocalizes with HSF1 in nuclear stress bodies (nSBs) upon heat shock (By similarity).   With evidence from 9 publications.
Acrosome   Translocates into the nucleus (monomeric catalytic subunit). The inactive holoenzyme is found in the cytoplasm. Distributed throughout the cytoplasm in meiotically incompetent oocytes. Associated to mitochondrion as meiotic competence is acquired. Aggregates around the germinal vesicles (GV) at the immature GV stage oocytes (By similarity). Colocalizes with MROH2B and TCP11 on the acrosome and tail regions in round spermatids and spermatozoa regardless of the capacitation status of the sperm (By similarity). Colocalizes with HSF1 in nuclear stress bodies (nSBs) upon heat shock (By similarity).   With evidence from 9 publications.
Length:
366
Number of predicted TMHs:
0
Exp number of AAs in TMHs:
32.8859600000001
Exp number, first 60 AAs:
0.00337
Total prob of N-in:
0.39446
outside
1  -  366
 
 

Population Genetic Test Statistics

Pi
209.269885
Theta
217.335283
Tajima's D
-0.15855
CLR
0.206851
CSRT
0.327033648317584
Interpretation
Uncertain

Multiple alignment of Orthologues

 
 

Gene Tree

 
 
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