Gene

KWMTBOMO07935  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA001165

Annotation

PREDICTED:_dynamin-1-like_protein_[Bombyx_mori]

Location

Bomo_Chr13(-):10462708-10478176      View in EpiBrowser

Full name

Dynamin-1-like protein      

Alternative Name

Dnm1p/Vps1p-like protein
Dynamin family member proline-rich carboxyl-terminal domain less
Dynamin-like protein
Dynamin-like protein 4
Dynamin-like protein IV
Dynamin-related protein 1

Location in the cell

Nuclear   Reliability : 1.99

CDS

ATGGAAGCTCTTATACCAGTTATTAATAAACTACAAGATGTGTTCAATACAGTTGGAGCTGATGCCATTCAATTACCCCAAATAGCTGTTTTAGGAACCCAGAGCTCTGGTAAAAGTTCAGTGATAGAAAGCCTGGTAGGTCGCTCATTTCTGCCTCGGGGTCCTGGGATAGTCACTCGGCGTCCTCTCATTCTCCAGTTAGTCTACAGTCCAAAAGAAGGCAAAGAGCATCGCTCAGCTGAAGAAGGTACTCTTAATCTAGAAGAATGGGGAAAGTTCCTACACACAAAAGACAAAATTTACACAAACTTTGAAGAGATAAGACAAGAAATAGAAAGAGAAACTGACCGTATGGCTGGCAGCAACAAGGGAATATCTCCAGAACCTATAAACTTGAAAATATATTCAACCACAGTTGTCAACCTTACTTTAGTCGATCTACCCGGTATAACAAAGGTGCCAATAGGTGATCAACCAGAAGACATAGAAAACCAAATCAGAAACCTTATTATTAAGTATATCTCTAACCCAAATTCCATTATATTAGCTGTAACAGCTGCTAATACAGACATGGCGACTAGTGAAGCCATCAAATTGGCTAAGGATGTGGACCCTGATGGCAGAAGGACATTGGCTGTTGTCACCAAACTTGATCTCATGGATGCAGGAACTGATGCAATAGATATTCTGTGTGGGCGCGTTATTCCGGTTAAACTAGGAATCATTGGCGTCGTAAATAGATCACAACAAGACATTATTGATAAGAAAACCATCACGGATGCACTGAAAGATGAAGCCACTTATCTCCAAAGGAAGTATCCCACTATAGCAACACGTAACGGAACTCCGTATTTAGCTAAAACATTGAATAGACTACTCATGCATCATATAAGAGATTGCCTACCAGAACTCAAGGTGCGTGTTAATGTAATGATATCACAATTCCAATCTCTTCTGAACTCCTACGGCGAAGATGTGTCGGACAAGTCTCAGACGTTATTGCAAATTATAACGAAATTCGCGAGTGCTTACTGTTCGACAATAGAAGGTACCGCCCGGAACATTGAGACCACTGAACTTTGTGGCGGCGCCAGAATCTGTTATATATTCCACGAGACTTTTGGACGCACTTTGGACTCCATACATCCTTTAGTAGGTTTGACCCGCATGGACATTTTGACCGCGATCCGCAACGCTACAGGCCCTAGACCGGCTTTATTCGTCCCTGAGGTATCTTTTGAGCTTCTGGTCAAGCGACAGATTAGAAGACTGGAAGACCCTTCGTTACGTTGTGTTGAACTGGTACACGAAGAAATGCAGAGGATCGTTCAGCACTGTGGCACAGAAGTCCAGCAGGAAATGCTACGTTTCCCACGCCTGCACCAGCGCATCGTCGACGTCGTCACGCAGCTGCTGAGGACTAGATTGCCTCCGACCAATTCTATGGTGGAGAACCTGGTGGCTATCGAGCTTGCGTACATAAACACGAAGCACCCCGACTTCCACCGCGAGGCGGCGCTGGTGTCCGGCCTGCTGAAGAGCGGCGACGACGACCACAGTCCCGTCTATAGACACAAGACTCCCAGGCCTTCTCCCTCACCGAATGTTCCCGCTATAACCGATGGTCACGAGAAATCTCCACAAAACATCAGCGAAACACCGTCGACCCCACAGATCAACGGAATGCCAGACAACAAGGCACTGACTCCTCAGAAGCCAGTGAACCTTCTGCCCGAAGTACCGATCCAAACATCACGTAAACTGTCAGAACGTGAACAACACGACTGTGACGTCATAGAGAGACTGATCAAGTCTTATTTCTACATCGTCCGGAAATCCATTCAGGACTCTGTACCAAAGGCTGTTATGCATTTCTTAGTGAATTTTGTGAAGGACAATCTGCAGTCCGAGCTCGTCACTCACCTGTACAAGTCTGACCAGGCCGAGAGCATGCTCAACGAGTCTGAGCACATCGCACAGCGGAGGAAGGAGGCCGCCGACATGCTAAAGGCTTTACAACGTGCCGGTCAAATCATAAGCGAGATCCGCGAGACGCACATGTGGTGA

Protein

MEALIPVINKLQDVFNTVGADAIQLPQIAVLGTQSSGKSSVIESLVGRSFLPRGPGIVTRRPLILQLVYSPKEGKEHRSAEEGTLNLEEWGKFLHTKDKIYTNFEEIRQEIERETDRMAGSNKGISPEPINLKIYSTTVVNLTLVDLPGITKVPIGDQPEDIENQIRNLIIKYISNPNSIILAVTAANTDMATSEAIKLAKDVDPDGRRTLAVVTKLDLMDAGTDAIDILCGRVIPVKLGIIGVVNRSQQDIIDKKTITDALKDEATYLQRKYPTIATRNGTPYLAKTLNRLLMHHIRDCLPELKVRVNVMISQFQSLLNSYGEDVSDKSQTLLQIITKFASAYCSTIEGTARNIETTELCGGARICYIFHETFGRTLDSIHPLVGLTRMDILTAIRNATGPRPALFVPEVSFELLVKRQIRRLEDPSLRCVELVHEEMQRIVQHCGTEVQQEMLRFPRLHQRIVDVVTQLLRTRLPPTNSMVENLVAIELAYINTKHPDFHREAALVSGLLKSGDDDHSPVYRHKTPRPSPSPNVPAITDGHEKSPQNISETPSTPQINGMPDNKALTPQKPVNLLPEVPIQTSRKLSEREQHDCDVIERLIKSYFYIVRKSIQDSVPKAVMHFLVNFVKDNLQSELVTHLYKSDQAESMLNESEHIAQRRKEAADMLKALQRAGQIISEIRETHMW

Description

Functions in mitochondrial and peroxisomal division. Mediates membrane fission through oligomerization into membrane-associated tubular structures that wrap around the scission site to constrict and sever the mitochondrial membrane through a GTP hydrolysis-dependent mechanism. The specific recruitment at scission sites is mediated by membrane receptors like MFF, MIEF1 and MIEF2 for mitochondrial membranes (PubMed:29899447). While the recruitment by the membrane receptors is GTP-dependent, the following hydrolysis of GTP induces the dissociation from the receptors and allows DNM1L filaments to curl into closed rings that are probably sufficient to sever a double membrane (PubMed:29899447). Through its function in mitochondrial division, ensures the survival of at least some types of postmitotic neurons, including Purkinje cells, by suppressing oxidative damage. Required for normal brain development, including that of cerebellum. Facilitates developmentally regulated apoptosis during neural tube formation. Required for a normal rate of cytochrome c release and caspase activation during apoptosis; this requirement may depend upon the cell type and the physiological apoptotic cues. Plays an important role in mitochondrial fission during mitosis (PubMed:26992161, PubMed:27301544, PubMed:27328748). Required for formation of endocytic vesicles. Proposed to regulate synaptic vesicle membrane dynamics through association with BCL2L1 isoform Bcl-X(L) which stimulates its GTPase activity in synaptic vesicles; the function may require its recruitment by MFF to clathrin-containing vesicles (PubMed:23792689). Required for programmed necrosis execution. Rhythmic control of its activity following phosphorylation at Ser-637 is essential for the circadian control of mitochondrial ATP production (PubMed:29478834).
Isoform 1: Inhibits peroxisomal division when overexpressed.
Isoform 4: Inhibits peroxisomal division when overexpressed.

Catalytic Activity

GTP + H2O = GDP + H(+) + phosphate

Subunit

Homotetramer; dimerizes through the N-terminal GTP-middle region of one molecule binding to the GED domain of another DNM1L molecule. Oligomerizes in a GTP-dependent manner to form membrane-associated tubules with a spiral pattern. Interacts with GSK3B and MARCH5. Interacts (via the GTPase and B domains) with UBE2I; the interaction promotes sumoylation of DNM1L, mainly in its B domain. Interacts with PPP3CA; the interaction dephosphorylates DNM1L and regulates its transition to mitochondria. Interacts with BCL2L1 isoform BCL-X(L) and CLTA; DNM1L and BCL2L1 isoform BCL-X(L) may form a complex in synaptic vesicles that also contains clathrin and MFF. Interacts with FIS1. Interacts with MIEF2 and MIEF1; GTP-dependent this regulates GTP hydrolysis and DNM1L oligomerization. Interacts with PGAM5; this interaction leads to dephosphorylation at Ser-656 and activation of GTPase activity and eventually to mitochondria fragmentation.

Similarity

Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family.

Keywords

3D-structure   Acetylation   Alternative splicing   Biological rhythms   Cell junction   Coated pit   Complete proteome   Cytoplasm   Cytoplasmic vesicle   Disease mutation   Endocytosis   Glycoprotein   Golgi apparatus   GTP-binding   Hydrolase   Isopeptide bond   Lipid-binding   Membrane   Mitochondrion   Mitochondrion outer membrane   Necrosis   Nucleotide-binding   Peroxisome   Phosphoprotein   Polymorphism   Reference proteome   S-nitrosylation   Synapse   Ubl conjugation  

Feature

chain  Dynamin-1-like protein
splice variant  In isoform 7.
sequence variant  In OPA5; changed localization to mitochondrion; impaired mitochondrial membrane fission.

Uniprot

A0A2A4J5N0   A0A2A4J6W8   A0A2A4J6P3   A0A2W1BP79   A0A2A4J700   A0A194Q8Q3   + More
A0A2A4J6Z4   A0A0N1IHE8   A0A0M8ZZL6   A0A2J7QJZ3   A0A2J7QJZ1   A0A336MNA8   A0A182NGN4   Q7PWP5   A0A026WPL7   D6WKF9   A0A182V7L9   A0A1S4GZK0   A0A1L8DWD6   B3N1Y0   A0A1L8DVW1   Q16WY5   A0A1W4V6M0   B4N0E4   E9IY57   A0A172QPL2   U5EY90   A0A1W4WV60   A0A1Y1KR18   A0A0J7P1U6   A0A0T6AZ42   B4I2M5   Q9VQE0   Q8IHG0   B4Q8H2   A0A3B0K8B8   A0A1A9V894   B4NWR9   A0A0L0CMA7   A0A291R8Z1   A0A1A9XER2   A0A1B0C0N6   B3NA31   A0A1B6LJA1   N6T304   A0A182H8F2   A0A1B0GJH3   R4WT42   A0A2S2R2A3   A0A0A9Y6C7   V5HVY8   A0A2H8TTV3   A0A224YLR3   L7M9U5   A0A1E1XIC0   A0A131Z3I1   A0A131XH17   A0A0N7Z9E4   T1HB68   A0A1E1XTS4   J9K4A0   A0A1J1I3V9   A0A222AIY1   A0A3L8DJV6   A0A069DWC3   A0A023FL20   A0A2A4J5E4   A0A2A4J686   A0A023FCG9   A0A1W0WAA4   A0A1D2N955   A0A226EGN1   A0A1D1UJF8   A0A146LBC4   W4YSL5   A0A1S3KBV8   A0A2C9K3W9   A0A1S3IMI4   A0A2R2MNP5   A0A1S3KBQ1   A0A2C9K3X3   O00429-5   A0A2K6G3S4   A0A287CS12   A0A2K5JXS8   A0A2K5RFV3   A0A091VV78   A0A2K6CWD2   A0A2K6LKV7   A0A2K5UCY0   A0A2I2Z6J7   A0A2K5E1K3   A0A2K6R8V7   A0A2J8WC95   K7CJF9   F7HM23   O00429   F1NPT0   A0A0P6K3Q2   A0A1W4ZHD1  

EC Number

3.6.5.5

Pubmed

28756777    26354079    12364791    24508170    18362917    19820115    + More
17994087    17510324    21282665    28004739    10731132    12537568    12537572    12537573    12537574    16110336    17569856    17569867    26109357    26109356    22936249    17550304    26108605    23537049    26483478    23691247    25401762    25765539    28797301    25576852    28503490    26830274    28049606    27129103    29209593    30249741    26334808    25474469    27289101    27649274    26823975    15562597    26383154    9348079    9731200    9570752    10749171    14702039    16541075    15489334    9422767    9472031    9786947    9915810    11514614    12499366    12618434    15208300    16874301    16936636    17015472    17301055    17553808    17606867    17460227    18695047    18088087    18669648    18838687    19413330    19407830    19638400    19411255    19690332    19342591    20688057    20068231    21269460    21508961    21701560    21406692    22265414    22223895    23921378    23186163    23283981    23792689    24275569    26122121    23530241    29478834    23584531    23977156    29899447    26604000    27145208    26992161    27301544    27328748    28969390    22398555    25362486    17431167    25319552    15592404   

EMBL

NWSH01002968    PCG67189.1    PCG67190.1    PCG67194.1    KZ149944    PZC76849.1    + More
PCG67193.1    KQ459299    KPJ01913.1    PCG67192.1    KQ460202    KPJ17179.1    KQ435793    KOX74157.1    NEVH01013548    PNF28898.1    PNF28900.1    UFQS01001724    UFQT01001724    SSX12012.1    SSX31530.1    AAAB01008984    EAA43354.4    KK107152    EZA57034.1    KQ971342    EFA03988.2    GFDF01003519    JAV10565.1    CH902667    EDV30590.1    GFDF01003518    JAV10566.1    CH477551    EAT39103.1    CH963920    EDW77557.1    GL766908    EFZ14479.1    KU245763    AND99762.1    GANO01000440    JAB59431.1    GEZM01078088    GEZM01078087    JAV63018.1    LBMM01000373    KMQ98988.1    LJIG01022485    KRT80330.1    CH480820    EDW54020.1    AE014134    BT133244    AAF51235.1    AFB40589.1    AGB92483.1    BT001269    AAN71025.1    CM000361    CM002910    EDX03489.1    KMY87696.1    OUUW01000006    SPP81876.1    CM000157    EDW87411.1    JRES01000186    KNC33478.1    KY082901    ATL63303.1    JXJN01023698    CH954177    EDV57494.1    GEBQ01016288    JAT23689.1    APGK01054222    KB741248    KB632305    ENN71933.1    ERL91868.1    JXUM01118366    JXUM01118367    JXUM01118368    KQ566177    KXJ70341.1    AJWK01021692    AJWK01021693    AJWK01021694    AJWK01021695    AK417897    BAN21112.1    GGMS01014913    MBY84116.1    GBHO01016403    GBRD01005441    JAG27201.1    JAG60380.1    GANP01004021    JAB80447.1    GFXV01005446    MBW17251.1    GFPF01007421    MAA18567.1    GACK01005101    JAA59933.1    GFAC01000329    JAT98859.1    GEDV01003252    JAP85305.1    GEFH01001757    JAP66824.1    GDKW01000779    JAI55816.1    ACPB03014269    GFAA01000724    JAU02711.1    ABLF02022564    CVRI01000038    CRK94284.1    KY563806    ASO76335.1    QOIP01000007    RLU20725.1    GBGD01000679    JAC88210.1    GBBK01002155    JAC22327.1    PCG67191.1    PCG67188.1    GBBI01000244    JAC18468.1    MTYJ01000154    OQV12100.1    LJIJ01000139    ODN01790.1    LNIX01000004    OXA55816.1    BDGG01000001    GAU89864.1    GDHC01012956    JAQ05673.1    AAGJ04011675    AB006965    AF061795    AF000430    AF151685    AK299926    AK291094    AK294533    AB209070    AC084824    AC087588    BC024590    AGTP01074868    AGTP01074869    KL411409    KFR07069.1    AQIA01010528    AQIA01010529    CABD030084725    CABD030084726    CABD030084727    CABD030084728    NDHI03003395    PNJ67374.1    GABC01000958    GABF01007044    GABD01006636    GABE01006730    NBAG03000401    JAA10380.1    JAA15101.1    JAA26464.1    JAA38009.1    PNI28698.1    JSUE03006471    JU320788    JU320789    JU320790    AFE64544.1    AADN05000523    AC145973    GEBF01004253    JAN99379.1   

Proteomes

UP000218220    UP000053268    UP000053240    UP000053105    UP000235965    UP000075884    + More
UP000007062    UP000053097    UP000007266    UP000075903    UP000007801    UP000008820    UP000192221    UP000007798    UP000192223    UP000036403    UP000001292    UP000000803    UP000000304    UP000268350    UP000078200    UP000002282    UP000037069    UP000092443    UP000092460    UP000008711    UP000019118    UP000030742    UP000069940    UP000249989    UP000092461    UP000015103    UP000007819    UP000183832    UP000279307    UP000094527    UP000198287    UP000186922    UP000007110    UP000085678    UP000076420    UP000005640    UP000233160    UP000005215    UP000233080    UP000233040    UP000053283    UP000233120    UP000233180    UP000233100    UP000001519    UP000233020    UP000233200    UP000006718    UP000000539    UP000192224   

PRIDE

Q9VQE0    Q8IHG0    O00429   

Pfam

PF01031   Dynamin_M        + More
PF02212   GED
PF00350   Dynamin_N
PF01607   CBM_14

Interpro

IPR027417   P-loop_NTPase        + More
IPR003130   GED       
IPR000375   Dynamin_central       
IPR030556   DNM1L       
IPR022812   Dynamin_SF       
IPR030381   G_DYNAMIN_dom       
IPR001401   Dynamin_GTPase       
IPR011993   PH-like_dom_sf       
IPR020850   GED_dom       
IPR019762   Dynamin_GTPase_CS       
IPR002557   Chitin-bd_dom       
IPR036508   Chitin-bd_dom_sf       

SUPFAM

SSF52540   SSF52540        + More
SSF57625   SSF57625       

Gene 3D

2.30.29.30    

CDD

cd08771  DLP_1

ProteinModelPortal

A0A2A4J5N0   A0A2A4J6W8   A0A2A4J6P3   A0A2W1BP79   A0A2A4J700   A0A194Q8Q3   + More
A0A2A4J6Z4   A0A0N1IHE8   A0A0M8ZZL6   A0A2J7QJZ3   A0A2J7QJZ1   A0A336MNA8   A0A182NGN4   Q7PWP5   A0A026WPL7   D6WKF9   A0A182V7L9   A0A1S4GZK0   A0A1L8DWD6   B3N1Y0   A0A1L8DVW1   Q16WY5   A0A1W4V6M0   B4N0E4   E9IY57   A0A172QPL2   U5EY90   A0A1W4WV60   A0A1Y1KR18   A0A0J7P1U6   A0A0T6AZ42   B4I2M5   Q9VQE0   Q8IHG0   B4Q8H2   A0A3B0K8B8   A0A1A9V894   B4NWR9   A0A0L0CMA7   A0A291R8Z1   A0A1A9XER2   A0A1B0C0N6   B3NA31   A0A1B6LJA1   N6T304   A0A182H8F2   A0A1B0GJH3   R4WT42   A0A2S2R2A3   A0A0A9Y6C7   V5HVY8   A0A2H8TTV3   A0A224YLR3   L7M9U5   A0A1E1XIC0   A0A131Z3I1   A0A131XH17   A0A0N7Z9E4   T1HB68   A0A1E1XTS4   J9K4A0   A0A1J1I3V9   A0A222AIY1   A0A3L8DJV6   A0A069DWC3   A0A023FL20   A0A2A4J5E4   A0A2A4J686   A0A023FCG9   A0A1W0WAA4   A0A1D2N955   A0A226EGN1   A0A1D1UJF8   A0A146LBC4   W4YSL5   A0A1S3KBV8   A0A2C9K3W9   A0A1S3IMI4   A0A2R2MNP5   A0A1S3KBQ1   A0A2C9K3X3   O00429-5   A0A2K6G3S4   A0A287CS12   A0A2K5JXS8   A0A2K5RFV3   A0A091VV78   A0A2K6CWD2   A0A2K6LKV7   A0A2K5UCY0   A0A2I2Z6J7   A0A2K5E1K3   A0A2K6R8V7   A0A2J8WC95   K7CJF9   F7HM23   O00429   F1NPT0   A0A0P6K3Q2   A0A1W4ZHD1  

PDB

5WP9     E-value=0,     Score=2406

KEGG

101744411    K17065 dynamin 1-like protein [EC:3.6.5.5] | (RefSeq) dynamin-1-like protein

PATHWAY

04214    Apoptosis - fly - Bombyx mori (domestic silkworm)

GO

GO:0016559   GO:0003924   GO:0005525   GO:0000266   GO:0031966   GO:0008017   GO:0008637   GO:1903146   GO:0043653   GO:0016020   GO:0061025   GO:0005737   GO:0003374   GO:0010821   GO:0048312   GO:0000281   GO:0007370   GO:0030382   GO:0051646   GO:0005739   GO:0090141   GO:0016185   GO:0090140   GO:0007005   GO:0098793   GO:0005875   GO:0006030   GO:0005576   GO:0008061   GO:0070585   GO:0060047   GO:0005096   GO:0070266   GO:0005741   GO:0051259   GO:0032991   GO:0005789   GO:1904666   GO:0005794   GO:1990910   GO:0031625   GO:0000139   GO:0050714   GO:0030742   GO:0070584   GO:0048488   GO:0051433   GO:0032459   GO:0030672   GO:2000302   GO:0005829   GO:0036466   GO:0005905   GO:1900063   GO:1900244   GO:0042803   GO:0098835   GO:0008289   GO:0043231   GO:0030054   GO:0048471   GO:0005903   GO:2001244   GO:0010637   GO:0001836   GO:0042802   GO:0090650   GO:0005783   GO:0043065   GO:0044877   GO:0017137   GO:1904579   GO:0061003   GO:1903578   GO:0005777   GO:0090200   GO:0097194   GO:0090149   GO:0048511   GO:0006816   GO:0051289   GO:1905395   GO:0010468   GO:0005874   GO:0030276   GO:0006811   GO:0043015   GO:0007169   GO:0006412   GO:0016876   GO:0043039  

PANTHER

PTHR11566:SF39   PTHR11566  

Subcellular location

Cytoplasm  
Cytosol  
Golgi apparatus  
Endomembrane system  
Mitochondrion outer membrane  
Peroxisome  
Membrane  
Clathrin-coated pit  
Cytoplasmic vesicle  
Secretory vesicle  
Synaptic vesicle membrane  

Length:

688

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.00426

Exp number, first 60 AAs:

0.00042

Total prob of N-in:

0.00033

outside

1  -  688

 

 

Pi

166.363831

Theta

175.842574

Tajima's D

0.022983

CLR

1876.536316

CSRT

0.375881205939703

Interpretation

Uncertain