Gene

KWMTBOMO07851

Pre Gene Modal

BGIBMGA000938

Annotation

PREDICTED:_probable_dolichyl_pyrophosphate_Glc1Man9GlcNAc2_alpha-1?3-glucosyltransferase_[Amyelois_transitella]

Location

Bomo_Chr13(+):8277843-8286072      View in EpiBrowser

Full name

Alpha-1,3-glucosyltransferase       + More
Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase      

Alternative Name

Asparagine-linked glycosylation protein 8 homolog
Dol-P-Glc:Glc(1)Man(9)GlcNAc(2)-PP-dolichyl alpha-1,3-glucosyltransferase
Dolichyl-P-Glc:Glc1Man9GlcNAc2-PP-dolichyl glucosyltransferase

Location in the cell

PlasmaMembrane   Reliability : 4.98

CDS

ATGATTACCCAAGTAGTACTAATAGTTACAGCCGTTAAGTTTATGTTCATACCATTGTATCATTCAACAGACTTTGAAGTGCATCGAAATTGGTTGGCTATCACGAATAATGTTTCCGTCGAAGAGTGGTATTACGAATCGACATCAGAGTGGACACTGGACTACCCTCCACTCTTTGCCTGGCTAGAATATGGCTTGTCTATTGTAGCAAAATATTTTGATCCCAAAATGATAAAAATAGAAAATCTAAATTATAAATCTGATATGGCCGTGCTATTCCAAAGATTATCTGTGATTTTTTTAGACTTTGTTTACATTTTTAGTGTTAAAAGTTGCTCAAATCTAGTCAGCAATGGAAAGCTATTAGTTTTTATTTTATTAGTGTCCAACCCTGGCTTGCTGATGGTTGATCATATACACTTTCAATATAATGGATTTTTATATGGTTTTCTTCTGTTTTCTATATCGAACATGATAAGGTGTAATCATCTACAAGCAGCATTCTGGTTTGCTATGTTACTTAACTTTAAACATATATTTTTATATATTGCCCCAGTGTATGTGGTATATTTGTTAAGAGCATACTGTTTCACTCTTTCATCAATAGACGGAGTTCACACAGCTTGGTATTCCTTTTCAATAACAAATTTTATTAAACTGGCATTTGTTGTTGTATCAGTATTTACTTTATCGTTTGGACCATTTTTAGACCATTTGAATCAGATTTTAGTCAGATTATTTCCATTTAAAAGAGGACTTTGCCACGCATATTGGGCTCCCAATTTCTGGGCTCTGTACAATTTAGTAACAAAATATGGAATTAACATACCGAAATCGGAAGCATCGATGACAGGAGGGCTAGTGCAAGAATACGACCATTCAGTTTTACCATCAATTACGCCGAGTTTCACTTTTATAATGACGTGTTTGTCTATGATGCCCGCATTGTACAAGATGTGGTGTCTTTGTGCGGACAGAAGATACAGACCTATTTGTTTTATAAGATGTATTGTCGTCTGTGCGACTTGTTCGTTTATGCTGGGTTGGCATGTTCACGAGAAAGCAATTTTGATGATTATAATACCGTTGAGTTTTCTGTCGGTTCTTGGCGACGTGGACGGAAGATTATTCATTTTACTCTCAACCGTTCAATCGTTCGACTCATCGAGTTGGAAGTTTGAAAATAAAAGTGAAATGGAGCGGAAGGAGTTGTTCATTCAGATTGGTGATTTGGCATTTTATTACCTTTAG

Protein

MITQVVLIVTAVKFMFIPLYHSTDFEVHRNWLAITNNVSVEEWYYESTSEWTLDYPPLFAWLEYGLSIVAKYFDPKMIKIENLNYKSDMAVLFQRLSVIFLDFVYIFSVKSCSNLVSNGKLLVFILLVSNPGLLMVDHIHFQYNGFLYGFLLFSISNMIRCNHLQAAFWFAMLLNFKHIFLYIAPVYVVYLLRAYCFTLSSIDGVHTAWYSFSITNFIKLAFVVVSVFTLSFGPFLDHLNQILVRLFPFKRGLCHAYWAPNFWALYNLVTKYGINIPKSEASMTGGLVQEYDHSVLPSITPSFTFIMTCLSMMPALYKMWCLCADRRYRPICFIRCIVVCATCSFMLGWHVHEKAILMIIIPLSFLSVLGDVDGRLFILLSTVQSFDSSSWKFENKSEMERKELFIQIGDLAFYYL

Description

Adds the second glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc(1)Man(9)GlcNAc(2)-PP-Dol before it is transferred to the nascent peptide (By similarity). Required for PKD1/Polycystin-1 maturation and localization to the plasma membrane of the primary cilia (By similarity).

Catalytic Activity

alpha-D-Glc-(1->3)-alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol + dolichyl beta-D-glucosyl phosphate = alpha-D-Glc-(1->3)-alpha-D-Glc-(1->3)-alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol + dolichyl phosphate + H(+)

Similarity

Belongs to the ALG6/ALG8 glucosyltransferase family.

Keywords

Alternative splicing   Complete proteome   Congenital disorder of glycosylation   Disease mutation   Endoplasmic reticulum   Glycosyltransferase   Membrane   Polymorphism   Reference proteome   Transferase   Transmembrane   Transmembrane helix  

Feature

chain  Alpha-1,3-glucosyltransferase
splice variant  In isoform 2.
sequence variant  In CDG1H; dbSNP:rs121908293.

Uniprot

A0A1E1WIM4   M4M269   A0A2H1VGR7   A0A2A4JTX3   A0A212ELX4   A0A1B6FV48   + More
A0A2J7PPT6   A0A1B6HEF3   A0A1W4WBF7   A0A1A7YLK7   A0A1B6BXI8   A0A1A8QQS1   W5KCM7   A0A1A8DXA2   A0A1A8FHY1   A0A1A8BZ40   A0A1S3KH68   A0A3Q1I1Z5   A0A1A8GX15   A0A3P8XWD8   A0A1A8KEN3   A0A1A8QZ63   Q6ZM97   Q566T2   A0A1W4ZUI4   A0A210QT88   A0A3B4CED4   A0A3B4WNL1   A0A1A8ADB6   A0A1S3HMK8   A0A3B3T263   A0A1B6D306   A0A1L8HB15   A0A3P8XW97   A0A3Q3NAA3   A0A1B6M1M6   A0A3B4VPT0   A0A3B5A2Y4   F7A881   A0A2U9B6Y2   Q5FVB1   A4IHH8   A0A2J8V0Q0   W5UBQ7   A0A3Q1FP80   L8HVM2   Q9BVK2-2   A0A2I2ZXK9   A0A2K6C3K5   A0A2K5TXY9   A0A2D0SYZ3   A0A2J8MBN0   A0A2K5J2Y7   A0A2K5P155   A0A2I3LFC5   A0A067RF74   A0A2K6QEG2   M3ZBQ1   A0A3Q0TH41   F1MV61   A0A3P8TYA6   A0A1S3RG10   Q0P5D9   A0A3B3DQC3   A0A3P8ZTD2   A0A0F8APD9   A0A2J8V0P2   A0A2K6C3F4   K7ARD9   Q6NRA2   G2HE43   A0A024R5K5   Q9BVK2   H2Q4H1   F6WKY0   G3R7I9   A0A2K6C3I2   G7PNB7   H9EVM6   A0A2R9ABB6   A0A2K5TXV6   A0A3Q7T9G9   K9KBB2   F6VZR9   A0A2I4CUA4   A0A2K5J2R6   A0A3Q3DS33   A0A096MVL3   A0A2K5P150   A0A2Y9KDL1   U6DG13   W5P9G0   A0A2I3HB56   A0A3Q3JGS2   A0A2K6QEE8   M3WZ59   I3L8Z7   A0A0D9QUY0   A0A3P9IC15   A0A2I3T0N0  

EC Number

2.4.1.-
2.4.1.265

Pubmed

22118469    25329095    25069045    23594743    28812685    29240929    + More
27762356    17431167    23127152    22751099    11124703    16554811    15489334    12480927    15235028    28375157    22398555    16136131    24845553    25362486    19393038    29451363    25835551    21484476    11181995    26871637    17495919    22002653    25319552    22722832    19892987    20809919    17975172    30723633    17554307   

EMBL

GDQN01004195    JAT86859.1    JQ747501    AGG36458.1    ODYU01002495    SOQ40043.1    + More
NWSH01000642    PCG75074.1    AGBW02013971    OWR42492.1    GECZ01015701    JAS54068.1    NEVH01022644    PNF18357.1    GECU01034650    GECU01026325    JAS73056.1    JAS81381.1    HADW01002224    HADX01008573    SBP30805.1    GEDC01031569    JAS05729.1    HAEI01006045    SBR95891.1    HAEA01009406    SBQ37886.1    HAEB01011906    SBQ58433.1    HADZ01007925    SBP71866.1    HAEC01007476    SBQ75614.1    HAED01010746    HAEE01010742    SBR30792.1    HAEG01014900    SBR98802.1    AL627325    CAE47759.1    CU928848    BC093349    BC164687    AAH93349.1    AAI64687.1    NEDP02002015    OWF51951.1    HADY01013735    HAEJ01017962    SBP52220.1    GEDC01017272    JAS20026.1    CM004469    OCT93293.1    GEBQ01010154    GEBQ01007057    JAT29823.1    JAT32920.1    JSUE03012997    JSUE03012998    CP026246    AWO99595.1    BC090105    AAH90105.1    BC135531    AAI35532.1    ABGA01113927    ABGA01113928    ABGA01113929    ABGA01113930    ABGA01113931    ABGA01113932    NDHI03003437    PNJ51094.1    JT411033    AHH39285.1    JH883095    ELR47374.1    AJ224875    AP002520    CH471076    BC001133    CABD030080370    CABD030080371    CABD030080372    CABD030080373    AQIA01021535    AQIA01021536    AACZ04016310    NBAG03000261    PNI56929.1    AHZZ02007190    KK852672    KDR18773.1    ADFV01111229    ADFV01111230    ADFV01111231    ADFV01111232    ADFV01111233    ADFV01111234    ADFV01111235    ADFV01111236    BC120179    KQ041556    KKF25557.1    PNJ51085.1    GABC01006954    JAA04384.1    BC070861    AAH70861.1    AK305007    BAK62001.1    KU178764    ALQ34222.1    EAW75051.1    GABF01010490    GABD01004338    GABE01006028    JAA11655.1    JAA28762.1    JAA38711.1    PNI56926.1    CM001289    EHH56612.1    JU322679    JU472505    JV044589    AFE66435.1    AFH29309.1    AFI34660.1    AJFE02013302    AJFE02013303    AJFE02013304    AJFE02013305    AJFE02013306    AJFE02013307    JL620308    AEP99295.1    HAAF01009009    CCP80833.1    AMGL01060851    AANG04002890    AEMK02000070    DQIR01105371    DQIR01122511    DQIR01144885    DQIR01168884    DQIR01190177    DQIR01190989    DQIR01191825    DQIR01196356    DQIR01200500    DQIR01250151    DQIR01293354    HDA60847.1    AQIB01134049    AQIB01134050    AQIB01134051   

Proteomes

UP000218220    UP000007151    UP000235965    UP000192223    UP000018467    UP000085678    + More
UP000265040    UP000265140    UP000000437    UP000192224    UP000242188    UP000261440    UP000261360    UP000261540    UP000186698    UP000261640    UP000261420    UP000261400    UP000006718    UP000246464    UP000001595    UP000257200    UP000005640    UP000001519    UP000233120    UP000233100    UP000221080    UP000002277    UP000233080    UP000233060    UP000028761    UP000027135    UP000233200    UP000001073    UP000261340    UP000009136    UP000265080    UP000087266    UP000261560    UP000002280    UP000009130    UP000240080    UP000286640    UP000002281    UP000192220    UP000264820    UP000248482    UP000002356    UP000261600    UP000011712    UP000008227    UP000029965    UP000265200   

PRIDE

Q9BVK2    Q0P5D9   

Pfam

PF03155   Alg6_Alg8

Interpro

IPR039487   ALG8        + More
IPR004856   Glyco_trans_ALG6/ALG8       

ProteinModelPortal

A0A1E1WIM4   M4M269   A0A2H1VGR7   A0A2A4JTX3   A0A212ELX4   A0A1B6FV48   + More
A0A2J7PPT6   A0A1B6HEF3   A0A1W4WBF7   A0A1A7YLK7   A0A1B6BXI8   A0A1A8QQS1   W5KCM7   A0A1A8DXA2   A0A1A8FHY1   A0A1A8BZ40   A0A1S3KH68   A0A3Q1I1Z5   A0A1A8GX15   A0A3P8XWD8   A0A1A8KEN3   A0A1A8QZ63   Q6ZM97   Q566T2   A0A1W4ZUI4   A0A210QT88   A0A3B4CED4   A0A3B4WNL1   A0A1A8ADB6   A0A1S3HMK8   A0A3B3T263   A0A1B6D306   A0A1L8HB15   A0A3P8XW97   A0A3Q3NAA3   A0A1B6M1M6   A0A3B4VPT0   A0A3B5A2Y4   F7A881   A0A2U9B6Y2   Q5FVB1   A4IHH8   A0A2J8V0Q0   W5UBQ7   A0A3Q1FP80   L8HVM2   Q9BVK2-2   A0A2I2ZXK9   A0A2K6C3K5   A0A2K5TXY9   A0A2D0SYZ3   A0A2J8MBN0   A0A2K5J2Y7   A0A2K5P155   A0A2I3LFC5   A0A067RF74   A0A2K6QEG2   M3ZBQ1   A0A3Q0TH41   F1MV61   A0A3P8TYA6   A0A1S3RG10   Q0P5D9   A0A3B3DQC3   A0A3P8ZTD2   A0A0F8APD9   A0A2J8V0P2   A0A2K6C3F4   K7ARD9   Q6NRA2   G2HE43   A0A024R5K5   Q9BVK2   H2Q4H1   F6WKY0   G3R7I9   A0A2K6C3I2   G7PNB7   H9EVM6   A0A2R9ABB6   A0A2K5TXV6   A0A3Q7T9G9   K9KBB2   F6VZR9   A0A2I4CUA4   A0A2K5J2R6   A0A3Q3DS33   A0A096MVL3   A0A2K5P150   A0A2Y9KDL1   U6DG13   W5P9G0   A0A2I3HB56   A0A3Q3JGS2   A0A2K6QEE8   M3WZ59   I3L8Z7   A0A0D9QUY0   A0A3P9IC15   A0A2I3T0N0  

KEGG

101742022    K03849 alpha-1,3-glucosyltransferase [EC:2.4.1.265] | (RefSeq) probable dolichyl pyrophosphate Glc1M

PATHWAY

00510    N-Glycan biosynthesis - Bombyx mori (domestic silkworm)
01100    Metabolic pathways - Bombyx mori (domestic silkworm)

GO

GO:0006486   GO:0006490   GO:0016021   GO:0005789   GO:0042283   GO:0016757   GO:0042281   GO:0006487   GO:0018279   GO:0000033   GO:0006488   GO:0004583   GO:0016758   GO:0008270   GO:0005506   GO:0030145   GO:0050906   GO:0005515   GO:0016818   GO:0003707   GO:0051537  

PANTHER

PTHR12413   PTHR12413:SF2  

Subcellular location

Endoplasmic reticulum membrane  

Length:

416

Number of predicted TMHs:

7

Exp number of AAs in TMHs:

167.04937

Exp number, first 60 AAs:

17.46333

Total prob of N-in:

0.93138

POSSIBLE N-term signal

sequence

inside

1  -  4

TMhelix

5  -  22

outside

23  -  90

TMhelix

91  -  109

inside

110  -  120

TMhelix

121  -  143

outside

144  -  178

TMhelix

179  -  201

inside

202  -  207

TMhelix

208  -  230

outside

231  -  301

TMhelix

302  -  324

inside

325  -  330

TMhelix

331  -  353

outside

354  -  416

 

 

Pi

190.539476

Theta

207.258602

Tajima's D

-1.073232

CLR

0.688547

CSRT

0.12284385780711

Interpretation

Uncertain