Gene

KWMTBOMO07687  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA000866

Annotation

aspartylglucosaminidase_[Bombyx_mori]

Location

Bomo_Chr13(+):4404431-4409397      View in EpiBrowser

Full name

N(4)-(Beta-N-acetylglucosaminyl)-L-asparaginase      

Alternative Name

Aspartylglucosaminidase
Glycosylasparaginase
N4-(N-acetyl-beta-glucosaminyl)-L-asparagine amidase

Location in the cell

Extracellular   Reliability : 1.748 Lysosomal   Reliability : 1.288

CDS

ATGAACCAAATAGGAATATTTTTAACCTATGTATTAGTTTATTTAACGAATGTTTGTTCCGAAACAAATATTCCCATAGTGATTACTACTTGGTCGTTCACAAATTCAACGGTTAAAGCTTGGGAAGTTCTAAATAACGGAGGCACGGCATTAGATGCCGTAGAGCAGGGCGCATCGGTCTGTGAAGACCAACAATGTGATGGTACTGTAGGATATGGTGGAAGTCCGGACGAAAACGGAGAAACTACATTAGATGCTCTTATTATGAATGGGAGAACTATGAATATTGGAGCTGTGGGTGGCTTGAGAAGGATCAAACATGCCATATCTGTTGCTAGACATGTATTGGACCACACAAAACATTCCTTTTTAGTTGGTGAGCTTGCCACCCAATTTGCAGTAGAAATGGGCTTTCAAGAAGAATCCCTCACTACCTCTGTATCAAAAAAACTATGGATGAAATGGCATAATGAACATCATTGTCAACCAAATTTCTGGCTGAATGTCAGTCCAGATCCCACTCGAAACTGTGGTCCATATCAGAAACACGAGTACTCTCATCATCTACTTAAGAAGAGCCATCTTAATGTGGATCGGTTCAACCATGACACGATAGGTATGGTGGCTATTGATCAACATGGTGATATTGCAGCTGGAACTTCAACAAATGGTGCTAAATATAAAATACCTGGAAGAATAGGTGATTCGCCGATTCCTGGGTCCGGCGCTTATGCTGACAATGATGTAGGCGGTGCTGCGGCGACTGGCGACGGCGATGTGATGTTGCGATTCCTTCCAAGTTTTTTAACAGTAGAAGAAATGCGCCGAGGCTCTTCACCTACTGATGCTGCTAAAACAGCAGTTGCGAGAATAGCGTCCCATTATCCGGATTTTATGGGAGCAGTTGTTGCACTGAAGAATGACGGCGAATACGGAGCGGCATGCCACGGAATAAACATGTTCCCTTATGTTGTATACGATAAAAAACATAGTTCCTATCAAATTGTTAACGTATCTTGTAGTTAA

Protein

MNQIGIFLTYVLVYLTNVCSETNIPIVITTWSFTNSTVKAWEVLNNGGTALDAVEQGASVCEDQQCDGTVGYGGSPDENGETTLDALIMNGRTMNIGAVGGLRRIKHAISVARHVLDHTKHSFLVGELATQFAVEMGFQEESLTTSVSKKLWMKWHNEHHCQPNFWLNVSPDPTRNCGPYQKHEYSHHLLKKSHLNVDRFNHDTIGMVAIDQHGDIAAGTSTNGAKYKIPGRIGDSPIPGSGAYADNDVGGAAATGDGDVMLRFLPSFLTVEEMRRGSSPTDAAKTAVARIASHYPDFMGAVVALKNDGEYGAACHGINMFPYVVYDKKHSSYQIVNVSCS

Catalytic Activity

H2O + N(4)-(beta-N-acetyl-D-glucosaminyl)-L-asparagine = H(+) + L-aspartate + N-acetyl-beta-D-glucosaminylamine

Subunit

Heterotetramer of two alpha and two beta chains or heterodimer of an alpha and a beta chain.

Similarity

Belongs to the Ntn-hydrolase family.

Keywords

Autocatalytic cleavage   Disulfide bond   Glycoprotein   Hydrolase   Lysosome   Protease  

Feature

chain  Glycosylasparaginase alpha chain

Uniprot

Q0PVE2   A0A2W1BNY3   A0A2A4JTX6   H9IUD6   S4PMM6   A0A194QYD1   + More
A0A194PDP9   A0A0L7KUV7   A0A3S2LP42   A0A2H1WU24   O02467   A0A2J7Q108   A0A067RDR4   A0A2H8TU61   A0A1Q3F673   B0X0J0   A0A1W4XUG5   K7J6P3   J9JXT9   A0A232EIS5   A0A1B6KE62   A0A0K2TB28   A0A1W6EW98   A0A0C9RGS9   C1BMI4   A0A154PJC3   A0A151I7R6   A0A1Z5L6S5   A0A1D1V9Z9   A0A293MS46   A0A158NKB0   T1KBF9   A0A2A3EG38   A0A151I0K3   A0A1S3IY47   A0A224YUC8   F4W8L1   A0A1S4FM69   A0A131YXC8   E0VAH9   V5IB86   A0A087ZYB5   Q16VX6   A0A087TQ27   A0A151WIT3   E9H0E1   A0A1Y1LZF9   V5HHM1   A0A1B6G619   A0A1S7IVP7   A0A336MN77   A0A0P6CZI8   E9IBU9   A0A0P6G277   A0A1S7IVQ5   U5ELS7   A0A182U1E4   A0A164WCC5   A0A195FCC1   A0A146LSA1   A0A182KSL7   A0A0L7R0K0   A0A0P5JM61   A0A182H9B9   A0A0P5N5L1   A0A195EIS6   Q7Q079   A0A131Y1T6   A0A182G2Y0   A0A0P6C1H5   A0A182SAM8   A0A0P5PRB9   A0A0P5I204   A0A182HQ38   A0A0K8SGT2   A0A182VMH5   A0A0P5S611   A0A0P5XV16   L7M0T3   A0A0P6HPG3   A0A0P5JND4   A0A0K8U659   A0A0A1WRN7   A0A0J7KUU9   A0A2K8JWQ3   A0A182XNB7   A0A1B6MAF8   A0A1J1I8P3   A0A0P5ZNV1   D2A367   A0A0P5MCE7   A0A0P5VID2   A0A182W952   A0A3M7RFD1   A0A0C9SFJ7   F4W945   A0A195EDV6   A0A034WLU4   K7J6P2   A0A0L0BNW9  

EC Number

3.5.1.26

Pubmed

28756777    19121390    23622113    26354079    26227816    8877373    + More
24845553    20075255    28648823    28528879    27649274    21347285    28797301    21719571    17510324    26830274    20566863    25765539    21292972    28004739    21282665    26823975    20966253    26483478    12364791    25576852    25830018    18362917    19820115    30375419    26131772    25348373    26108605   

EMBL

DQ683192    ABG81362.1    KZ149946    PZC76769.1    NWSH01000677    PCG74860.1    + More
BABH01001239    BABH01001240    GAIX01000096    JAA92464.1    KQ460947    KPJ10548.1    KQ459606    KPI91143.1    JTDY01005587    KOB66856.1    RSAL01000576    RVE41372.1    ODYU01010667    SOQ55924.1    S83425    NEVH01019960    PNF22271.1    KK852761    KDR17001.1    GFXV01004913    MBW16718.1    GFDL01011989    JAV23056.1    DS232239    EDS38151.1    ABLF02031677    NNAY01004159    OXU18269.1    GEBQ01030272    JAT09705.1    HACA01005315    CDW22676.1    KY563608    ARK20017.1    GBYB01006211    JAG75978.1    BT075813    ACO10237.1    KQ434912    KZC11398.1    KQ978406    KYM94138.1    GFJQ02003854    JAW03116.1    BDGG01000004    GAU98464.1    GFWV01018618    MAA43346.1    ADTU01018761    CAEY01001948    KZ288266    PBC30116.1    KQ976601    KYM79426.1    GFPF01010071    MAA21217.1    GL887908    EGI69502.1    GEDV01004714    JAP83843.1    DS235006    EEB10385.1    GANP01015872    JAB68596.1    CH477579    EAT38737.1    KK116257    KFM67216.1    KQ983082    KYQ47721.1    GL732580    EFX74790.1    GEZM01045493    JAV77780.1    GANP01011495    JAB72973.1    GECZ01011928    JAS57841.1    LC092877    BAX00386.1    UFQS01000828    UFQT01000828    SSX07095.1    SSX27438.1    GDIQ01085211    JAN09526.1    GL762137    EFZ21943.1    GDIQ01039616    JAN55121.1    LC093049    BAX00387.1    GANO01001286    JAB58585.1    LRGB01001265    KZS13141.1    KQ981693    KYN37689.1    GDHC01009319    JAQ09310.1    KQ414670    KOC64380.1    GDIQ01198109    JAK53616.1    JXUM01120177    KQ566367    KXJ70182.1    GDIQ01146990    JAL04736.1    KQ978881    KYN27764.1    AAAB01008986    EAA00283.4    GEFM01003354    JAP72442.1    JXUM01140626    KQ569095    KXJ68762.1    GDIP01006126    JAM97589.1    GDIQ01145589    JAL06137.1    GDIQ01224908    JAK26817.1    APCN01003291    GBRD01013333    JAG52493.1    GDIQ01098300    JAL53426.1    GDIP01066913    JAM36802.1    GACK01007394    JAA57640.1    GDIQ01024022    JAN70715.1    GDIQ01222306    JAK29419.1    GDHF01030273    JAI22041.1    GBXI01012568    JAD01724.1    LBMM01002883    KMQ94247.1    KY031096    ATU82847.1    GEBQ01007052    JAT32925.1    CVRI01000044    CRK96595.1    GDIP01042113    JAM61602.1    KQ971338    EFA01956.1    GDIQ01161980    JAK89745.1    GDIP01099560    JAM04155.1    REGN01003513    RNA22220.1    GBZX01000037    JAG92703.1    GL888002    EGI69227.1    KQ979039    KYN23291.1    GAKP01003625    JAC55327.1    JRES01001589    KNC21668.1   

Proteomes

UP000218220    UP000005204    UP000053240    UP000053268    UP000037510    UP000283053    + More
UP000235965    UP000027135    UP000002320    UP000192223    UP000002358    UP000007819    UP000215335    UP000076502    UP000078542    UP000186922    UP000005205    UP000015104    UP000242457    UP000078540    UP000085678    UP000007755    UP000009046    UP000005203    UP000008820    UP000054359    UP000075809    UP000000305    UP000075902    UP000076858    UP000078541    UP000075882    UP000053825    UP000069940    UP000249989    UP000078492    UP000007062    UP000075901    UP000075840    UP000075903    UP000036403    UP000076407    UP000183832    UP000007266    UP000075920    UP000276133    UP000037069   

PRIDE

Q0PVE2    H9IUD6   

Pfam

PF01112   Asparaginase_2        + More
PF00060   Lig_chan

Interpro

IPR000246   Peptidase_T2        + More
IPR029055   Ntn_hydrolases_N       
IPR001320   Iontro_rcpt       

SUPFAM

SSF56235   SSF56235       

ProteinModelPortal

Q0PVE2   A0A2W1BNY3   A0A2A4JTX6   H9IUD6   S4PMM6   A0A194QYD1   + More
A0A194PDP9   A0A0L7KUV7   A0A3S2LP42   A0A2H1WU24   O02467   A0A2J7Q108   A0A067RDR4   A0A2H8TU61   A0A1Q3F673   B0X0J0   A0A1W4XUG5   K7J6P3   J9JXT9   A0A232EIS5   A0A1B6KE62   A0A0K2TB28   A0A1W6EW98   A0A0C9RGS9   C1BMI4   A0A154PJC3   A0A151I7R6   A0A1Z5L6S5   A0A1D1V9Z9   A0A293MS46   A0A158NKB0   T1KBF9   A0A2A3EG38   A0A151I0K3   A0A1S3IY47   A0A224YUC8   F4W8L1   A0A1S4FM69   A0A131YXC8   E0VAH9   V5IB86   A0A087ZYB5   Q16VX6   A0A087TQ27   A0A151WIT3   E9H0E1   A0A1Y1LZF9   V5HHM1   A0A1B6G619   A0A1S7IVP7   A0A336MN77   A0A0P6CZI8   E9IBU9   A0A0P6G277   A0A1S7IVQ5   U5ELS7   A0A182U1E4   A0A164WCC5   A0A195FCC1   A0A146LSA1   A0A182KSL7   A0A0L7R0K0   A0A0P5JM61   A0A182H9B9   A0A0P5N5L1   A0A195EIS6   Q7Q079   A0A131Y1T6   A0A182G2Y0   A0A0P6C1H5   A0A182SAM8   A0A0P5PRB9   A0A0P5I204   A0A182HQ38   A0A0K8SGT2   A0A182VMH5   A0A0P5S611   A0A0P5XV16   L7M0T3   A0A0P6HPG3   A0A0P5JND4   A0A0K8U659   A0A0A1WRN7   A0A0J7KUU9   A0A2K8JWQ3   A0A182XNB7   A0A1B6MAF8   A0A1J1I8P3   A0A0P5ZNV1   D2A367   A0A0P5MCE7   A0A0P5VID2   A0A182W952   A0A3M7RFD1   A0A0C9SFJ7   F4W945   A0A195EDV6   A0A034WLU4   K7J6P2   A0A0L0BNW9  

PDB

1APZ     E-value=6.34926e-54,     Score=532

KEGG

751092    K01444 N4-(beta-N-acetylglucosaminyl)-L-asparaginase [EC:3.5.1.26] | (RefSeq) aspartylglucosaminidas

PATHWAY

00511    Other glycan degradation - Bombyx mori (domestic silkworm)
04142    Lysosome - Bombyx mori (domestic silkworm)

GO

GO:0016787   GO:0005764   GO:0008233   GO:0003948   GO:0016021   GO:0005737   GO:0006517   GO:0004970   GO:0004518   GO:0007018   GO:0008017   GO:0004402   GO:0016573   GO:0008270   GO:0022891   GO:0003707   GO:0051537  

PANTHER

PTHR10188  

Subcellular location

Lysosome  

SignalP

Position:   1 - 20,         Likelihood:  0.965139

 

 

Length:

341

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

3.57914000000001

Exp number, first 60 AAs:

3.57087

Total prob of N-in:

0.15804

outside

1  -  341

 

 

Pi

25.600469

Theta

155.805145

Tajima's D

-1.684414

CLR

1.987734

CSRT

0.0380980950952452

Interpretation

Uncertain