Gene

KWMTBOMO07376  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA004956

Annotation

L-isoaspartyl_protein_carboxyl_methyltransferase_[Bombyx_mori]

Location

Bomo_Chr12(-):11535703-11537489      View in EpiBrowser

Full name

Protein-L-isoaspartate O-methyltransferase       + More
Protein-L-isoaspartate(D-aspartate) O-methyltransferase      

Alternative Name

L-isoaspartyl protein carboxyl methyltransferase
Protein L-isoaspartyl/D-aspartyl methyltransferase
Protein-beta-aspartate methyltransferase

Location in the cell

Cytoplasmic   Reliability : 1.599 Mitochondrial   Reliability : 1.344

CDS

ATGGCATGGAGAAGCCACGGAGCAAATAATGTGGACCTAATACGTAATTTAAGAACTAACGGCATAATAAAATCGGACACCGTCGCAAATGCAATGCTAGCTGTTGATCGGAAAAATTACTGTCCTTCTTCTCCATACCAGGACTCTCCTCAATCTATTGGATTCTCTGCAACAATCAGCGCTCCCCACATGCATGCTCATGCATTAGAAAAGTTAAAAAACCAGCTAGTACCAGGAGAAAAAGCTTTAGATGTTGGTTCGGGGTCTGGTTACCTCACTGCCTGTATGGCCATGATGTTAGGAGAAACTGGCCGGGTAGTTGGGATAGAACATATATCAGAACTTGTGAATTTGGCAACCAAAAACATCCAAAATGACAACCCAAGCTTACTCTCATCTGAGAGGATTAAACTTGTAGTTGGTGATGGTCGTCTCGGTTATCCATCTGAAGCCCCTTACAGTGCAATTCATGTCGGGGCTGCAGCACCCACATTACCACAAGCTCTAATTGATCAATTGAAACCGGGAGGGAGGCTAATAGTTCCTGTAGGGCCTGAGGGTGGTGAACAACATTTGACTCAGGTAAGATTTGATTTTGGTATAATTATACAATTACAATGA

Protein

MAWRSHGANNVDLIRNLRTNGIIKSDTVANAMLAVDRKNYCPSSPYQDSPQSIGFSATISAPHMHAHALEKLKNQLVPGEKALDVGSGSGYLTACMAMMLGETGRVVGIEHISELVNLATKNIQNDNPSLLSSERIKLVVGDGRLGYPSEAPYSAIHVGAAAPTLPQALIDQLKPGGRLIVPVGPEGGEQHLTQVRFDFGIIIQLQ

Description

Catalyzes the methyl esterification of L-isoaspartyl and D-aspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins. Acts on EIF4EBP2, microtubule-associated protein 2, calreticulin, clathrin light chains a and b, Ubiquitin carboxyl-terminal hydrolase isozyme L1, phosphatidylethanolamine-binding protein 1, stathmin, beta-synuclein and alpha-synuclein.

Catalytic Activity

[protein]-L-beta-isoaspartate + S-adenosyl-L-methionine = [protein]-L-beta-isoaspartate alpha-methyl ester + S-adenosyl-L-homocysteine

Subunit

Monomer.

Similarity

Belongs to the methyltransferase superfamily. L-isoaspartyl/D-aspartyl protein methyltransferase family.

Keywords

Acetylation   Alternative splicing   Complete proteome   Cytoplasm   Direct protein sequencing   Methyltransferase   Reference proteome   S-adenosyl-L-methionine   Transferase   3D-structure   Disulfide bond   Polymorphism  

Feature

chain  Protein-L-isoaspartate O-methyltransferase
splice variant  In isoform 2.
sequence variant  In dbSNP:rs4816.

Uniprot

H9J615   Q2F5Q4   A0A2H1VND8   A0A2A4IT85   S4P2E9   A0A212F3I2   + More
A0A194R832   A0A194PTX6   A0A0T6AWZ0   E0VP09   A0A1B6HU82   A0A1B6MTD5   A0A1E1XDL2   A0A1B6FE94   C3Y0Q2   A0A023FQP3   L7LWS2   A0A1Y1LK39   D2A0T1   W4YGK2   A0A087TWX3   F7FVJ3   K9K455   A0A224XNC7   Q545L9   P23506   P23506-2   A0A1W4XNB0   A0A3Q0T897   A0A0P4W7I5   A0A3R7MBQ4   A0A1B6CBF5   F7D432   A0A2K6M1H9   A0A2K5Y1X6   A0A2I3MDP6   A0A2K5J3D5   F7GFV8   P22061-2   A0A061IM27   B9EQH3   E9PWE0   A0A0N7ZBD7   A0A096LUD5   A0A3B3UZR2   A0A384MDK7   P22061   P22062   A0A2J7R908   A0A1X7UY60   A0A3B3WZ96   E0CYV0   A0A1B6E8E2   F7A5R3   A0A2K6DB67   A0A2K5TKB8   G1RZE6   A0A2K6R8T3   A0A2K5MXW6   A0A2I3RQX8   A0A3Q2I233   A0A1B6CEA9   H9F8T0   Q4R5H0   A0A3B4H071   A0A3P8QHP4   A0A3P9BH93   A0A286XHE1   G2HIC2   A0A1U7Q3J9   A0A0B8RPN2   A0A3Q4H917   J3RZ00   F6YGL3   A0A060WK93   G8F1J4   A0A067QYM2   A0A3Q2HEG2   A0A0A0MRJ6   G1MVQ6   H9GKF5   H7BY58   A0A2I3H3H4   A0A2J8XAB5   K7B228   A0A2J8PRY9   A0A384CFN2   A0A2K6DAZ6   A0A2K6M1G7   A0A2K5Y1Z6   A0A0D9RXE2   A0A096NW19   A0A2K6R8P6   A0A2K5MXV8   A0A2K5J3N4   I7G7X4   M3WYS3   G1NXR2   A0A2J8XA81   X1WIT1  

EC Number

2.1.1.77

Pubmed

19121390    23622113    22118469    26354079    20566863    28503490    + More
18563158    25576852    28004739    18362917    19820115    17495919    10349636    11042159    11076861    11217851    12466851    12040188    16141073    1511895    15489334    8037467    16923807    21183079    20424163    19468303    17431167    2684970    1339271    7592526    14702039    14574404    8914929    3167043    8074695    1998518    24275569    25944712    10496068    11792715    11847284    21269460    23929341    20433749    2730663    8263531    19892987    25362486    16136131    25319552    25186727    21993624    21484476    25476704    23025625    18464734    24755649    22002653    24845553    20838655    21881562    17194215    17975172   

EMBL

BABH01029870    DQ311369    ABD36313.1    ODYU01003476    SOQ42316.1    NWSH01006888    + More
PCG63197.1    GAIX01012050    JAA80510.1    AGBW02010564    OWR48284.1    KQ460845    KPJ12026.1    KQ459592    KPI96767.1    LJIG01022645    KRT79467.1    DS235354    EEB15115.1    GECU01029470    JAS78236.1    GEBQ01016826    GEBQ01000775    JAT23151.1    JAT39202.1    GFAC01001880    JAT97308.1    GECZ01021345    JAS48424.1    GG666479    EEN66035.1    GBBK01001542    JAC22940.1    GACK01008784    JAA56250.1    GEZM01059723    JAV71387.1    KQ971338    EFA01639.1    AAGJ04105232    AAGJ04105233    AAGJ04105234    AAGJ04105235    AAGJ04105236    KK117123    KFM69612.1    JL616274    AEH58481.1    GFTR01003832    JAW12594.1    AK006162    CH466562    BAB24438.1    EDL03548.1    M60320    M84684    BC049613    BC058966    S72473    GDRN01085237    JAI61361.1    QCYY01001513    ROT77524.1    GEDC01026514    GEDC01010110    GEDC01003382    JAS10784.1    JAS27188.1    JAS33916.1    AC156391    AHZZ02025580    AHZZ02025581    JSUE03031939    JSUE03031940    M93008    M93009    D25545    D25546    D25547    D13892    AK289724    AL355312    CH471051    BC007501    BC008748    U49740    S73902    S73903    S73905    KE667778    ERE84887.1    BT057898    ACM09770.1    GDRN01085238    JAI61360.1    AYCK01008755    GQ891314    ADO22176.1    M26686    D11475    BC088417    NEVH01006721    PNF37302.1    GEDC01024979    GEDC01023933    GEDC01003121    JAS12319.1    JAS13365.1    JAS34177.1    AQIA01050235    AQIA01050236    ADFV01166511    ADFV01166512    ADFV01166513    ADFV01166514    ADFV01166515    ADFV01166516    ADFV01166517    ADFV01166518    ADFV01166519    AACZ04054481    GEDC01025557    GEDC01016265    GEDC01015593    GEDC01004460    JAS11741.1    JAS21033.1    JAS21705.1    JAS32838.1    JU327283    JU327284    AFE71039.1    AB169573    AAKN02044027    AAKN02044028    AAKN02044029    AAKN02044030    AK306486    BAK63480.1    GBSH01002007    JAG67019.1    JU174313    AFJ49839.1    AAPN01156880    AAPN01156881    AAPN01156882    AAPN01156883    AAPN01156884    AAPN01156885    AAPN01156886    FR904489    CDQ64975.1    JH290635    EHH25493.1    KK852870    KDR14599.1    AAWZ02022540    NDHI03003369    PNJ78963.1    GABC01009986    GABF01007897    GABD01006991    NBAG03000210    JAA01352.1    JAA14248.1    JAA26109.1    PNI86793.1    PNI86792.1    AQIB01120769    AQIB01120770    AQIB01120771    AQIB01120772    AQIB01120773    AQIB01120774    AQIB01120775    AQIB01120776    AQIB01120777    AQIB01120778    AB170294    BAE87357.1    AANG04000169    AAPE02018693    AAPE02018694    PNJ78961.1    ABLF02040061   

Proteomes

UP000005204    UP000218220    UP000007151    UP000053240    UP000053268    UP000009046    + More
UP000001554    UP000007266    UP000007110    UP000054359    UP000002280    UP000000589    UP000192223    UP000261340    UP000283509    UP000233180    UP000233140    UP000028761    UP000233080    UP000006718    UP000005640    UP000030759    UP000087266    UP000028760    UP000261500    UP000002494    UP000235965    UP000007879    UP000261480    UP000002281    UP000233120    UP000233100    UP000001073    UP000233200    UP000233060    UP000002277    UP000261460    UP000265100    UP000265160    UP000005447    UP000189706    UP000261580    UP000002279    UP000193380    UP000027135    UP000001645    UP000001646    UP000261680    UP000029965    UP000011712    UP000001074    UP000007819   

PRIDE

H9J615    Q2F5Q4    P23506    F7D432    P22061    E9PWE0    + More
P22062    E0CYV0    F7A5R3    Q4R5H0    A0A0A0MRJ6    H7BY58   

Interpro

IPR000682   PCMT        + More
IPR029063   SAM-dependent_MTases       

SUPFAM

SSF53335   SSF53335       

ProteinModelPortal

H9J615   Q2F5Q4   A0A2H1VND8   A0A2A4IT85   S4P2E9   A0A212F3I2   + More
A0A194R832   A0A194PTX6   A0A0T6AWZ0   E0VP09   A0A1B6HU82   A0A1B6MTD5   A0A1E1XDL2   A0A1B6FE94   C3Y0Q2   A0A023FQP3   L7LWS2   A0A1Y1LK39   D2A0T1   W4YGK2   A0A087TWX3   F7FVJ3   K9K455   A0A224XNC7   Q545L9   P23506   P23506-2   A0A1W4XNB0   A0A3Q0T897   A0A0P4W7I5   A0A3R7MBQ4   A0A1B6CBF5   F7D432   A0A2K6M1H9   A0A2K5Y1X6   A0A2I3MDP6   A0A2K5J3D5   F7GFV8   P22061-2   A0A061IM27   B9EQH3   E9PWE0   A0A0N7ZBD7   A0A096LUD5   A0A3B3UZR2   A0A384MDK7   P22061   P22062   A0A2J7R908   A0A1X7UY60   A0A3B3WZ96   E0CYV0   A0A1B6E8E2   F7A5R3   A0A2K6DB67   A0A2K5TKB8   G1RZE6   A0A2K6R8T3   A0A2K5MXW6   A0A2I3RQX8   A0A3Q2I233   A0A1B6CEA9   H9F8T0   Q4R5H0   A0A3B4H071   A0A3P8QHP4   A0A3P9BH93   A0A286XHE1   G2HIC2   A0A1U7Q3J9   A0A0B8RPN2   A0A3Q4H917   J3RZ00   F6YGL3   A0A060WK93   G8F1J4   A0A067QYM2   A0A3Q2HEG2   A0A0A0MRJ6   G1MVQ6   H9GKF5   H7BY58   A0A2I3H3H4   A0A2J8XAB5   K7B228   A0A2J8PRY9   A0A384CFN2   A0A2K6DAZ6   A0A2K6M1G7   A0A2K5Y1Z6   A0A0D9RXE2   A0A096NW19   A0A2K6R8P6   A0A2K5MXV8   A0A2K5J3N4   I7G7X4   M3WYS3   G1NXR2   A0A2J8XA81   X1WIT1  

PDB

1KR5     E-value=1.68546e-71,     Score=681

KEGG

692947    K00573 protein-L-isoaspartate(D-aspartate) O-methyltransferase [EC:2.1.1.77] | (RefSeq) L-isoasparty

GO

GO:0004719   GO:0016021   GO:0005737   GO:0005829   GO:0043005   GO:0043204   GO:0006479   GO:0046500   GO:0008757   GO:0031526   GO:0005615   GO:0046498   GO:0010667   GO:0016323   GO:0008168   GO:0045296   GO:1903561   GO:0030091   GO:0070062   GO:0045471   GO:0071456   GO:0007568   GO:0006464   GO:0003697   GO:0004520   GO:0006281   GO:0006259   GO:0043022   GO:0005488   GO:0003707   GO:0051537  

PANTHER

PTHR11579  

Subcellular location

Cytoplasm  

Length:

206

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.04444

Exp number, first 60 AAs:

0.00127

Total prob of N-in:

0.19568

outside

1  -  206

 

 

Pi

27.708856

Theta

22.874215

Tajima's D

0.648267

CLR

0.174038

CSRT

0.56367181640918

Interpretation

Uncertain