Gene

KWMTBOMO07274  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA010644

Annotation

aubergine_protein_[Bombyx_mori]

Location

Bomo_Chr12(+):9843868-9858154      View in EpiBrowser

Full name

Piwi-like protein Siwi      

Location in the cell

Nuclear   Reliability : 2.152

CDS

ATGTCAGAACCGAGAGGTAGAGGACGAGCTCGAGGACGCGCTGGTCGTGGTGGTGATGGAGGCGGACCAGCACCACGACGTCCAGGGGAACAAGCAGGCCCATCACAACAATCTATGCCACCTGGTCCTAGACCTCAACCTCCATCTGGCTGGGGACCACAATCCAGTGTTCCTCCAGTAAGAGCTGGTGTCCCGACACCAACAGCACAAGCGGGTCGCGCCTCACACCGTGTTACTCCCACTACACATGAACATCCAGGAGATATCGATGTGCAACAACGCATGCAAAAATTAGAACTGGGACCCCATTCATCTGGAGGTGGTGATGCATCATCCGTAGTTGGCCGTGGCTCTCGACGAGGGGGTGGAAGAGTCCTTCCTGAAACTATTTCCATCTTGCGCACACGTCCAGAAGCTGTAACATCTAAGAAAGGTACTTCGGGTACTCCCCTGGATCTGCTTGCCAACTATTTTACTGTTGAAACAACTCCCAAATGGGGCCTGTATCAATATCACGTCGATATTTCTCCAGAAGAAGACAGCACTGGAGTCCGAAAGGCACTGATGCGTGTTCACAGCAAAACACTCGGAGGATATCTCTTTGATGGCACTGTGTTGTACACTGTAAATCGTCTGCATCCTGATCCGATGGAGCTATATTCTGATCGTAAAACGGATAACGAACGTATGCGAATCTTAATTAAGCTGACCTGCGAAGTTAGCCCTGGAGACTATCACTATATACAAATCTTTAACATCATTATAAGGAAATGCTTTAATTTATTGAAGCTACAGCTTATGGGACGGGATTATTTTGACCCTGAAGCAAAGATTGATATACCAGAATTCAAGCTTCAAATTTGGCCAGGATATAAAACGACAATCAATCAATATGAAGATCGTTTGTTGTTGGTCACTGAAATCGCCCATAAGGTGCTTCGCATGGACACTGTTCTTCAAATGTTGAGTGAATATGCCGCAACAAAAGGCAATAATTACAAAAAAATATTTTTAGAAGATGTTGTTGGAAAAATTGTAATGACTGACTACAATAAAAGAACTTACAGAGTTGACGATGTAGCTTGGAATGTGTCGCCGAAGTCCACATTTAAAATGCGTGACGAAAACATTACCTATATTGAATACTATTATAAGAAATACAATCTACGCATTCAAGATCCGGGTCAACCGCTTCTGATTTCGCGGTCGAAGCCGCGTGAGATTCGTGCGGGTCTGCCTGAGTTGATATATCTAGTGCCAGAACTGTGCCGTCAGACCGGCCTCTCCGACGAGATGCGAGCTAATTTCAAGCTGATGCGCTCATTGGACGTCCATACAAAGATTGGGCCCGACAAGCGCATTGAAAAGCTTAATAATTTCAACAGGAGATTCACAAGCACACCTGAAGTTGTTGAAGAACTGGCAACATGGTCGTTGAAACTATCTAAGGAACTGGTAAAAATAAAGGGTCGCCAACTTCCACCTGAAAATATAATACAAGCAAATAATGTTAAATACCCTGCAGGAGACACCACCGAGGGCTGGACGCGAGATATGAGATCGAAGCATCTCCTGGCTATTGCCCAACTAAACTCTTGGGTAGTGATCACCCCAGAAAGACAACGCCGTGATACAGAGTCGTTCATTGATCTCATAATAAAAACTGGCGGAGGTGTTGGTTTTCGAATGAGAAGCCCTGACTTAGTTGTGATTCGTCATGACGGTCCGATAGAATACGCTAATATGTGTGAAGAAGTGATTGCAAGGAAAAATCCTGCCCTGATACTGTGCGTTCTGGCTAGGAATTATGCTGATAGGTATGAAGCCATAAAAAAGAAATGCACTGTAGACCGAGCCGTGCCGACGCAGGTAGTCTGCGCTCGTAACATGAGTAGCAAGTCGGCTATGTCAATTGCTACAAAAGTTGCTATTCAAATAAACTGTAAGCTTGGGGGTTCTCCGTGGACAGTAGATATCCCACTGCCGTCACTGATGGTGGTCGGGTACGACGTGTGCCACGACACTCGTTCGAAAGAAAAGAGCTTCGGGGCCTTCGTAGCCACCCTCGACAAACAGATGACTCAGTACTATTCAATCGTCAATGCGCACACTTCCGGAGAAGAACTCAGCTCTCACATGGGTTTCAATATCGCGTCCGCTGTCAAAAAATTCCGCGAGAAAAACGGGACCTACCCAGCCCGAATCTTCATTTACCGCGACGGTGTCGGTGATGGTCAAATCCCATACGTGCATAGCCATGAAGTTGCTGAGATAAAAAAGAAATTGGCAGAAATTTATGCAGGAGTCGAAATAAAGTTGGCATTCATTATTGTGTCGAAACGTATTAACACCCGAATCTTCGTTCAACGTGGTCGCAGCGGAGAAAATCCACGCCCTGGCACAGTGATTGACGACGTCGTCACCTTGCCTGAACGGTATGATTTCTATCTGGTTTCACAAAATGTACGTGAAGGTACCATTGCTCCTACCTCATACAATGTGATTGAAGATACAACAGGACTCAATCCTGATCGTATCCAACGTTTGACCTACAAACTGACACACTTGTATTTCAACTGCTCTTCCCAAGTTCGCGTCCCATCGGTCTGCCAATATGCCCACAAACTTGCATTCCTTGCAGCTAACAGTCTGCACAACCAACCGCATTATTCATTGAATGAAACTTTATATTTCCTCTAA

Protein

MSEPRGRGRARGRAGRGGDGGGPAPRRPGEQAGPSQQSMPPGPRPQPPSGWGPQSSVPPVRAGVPTPTAQAGRASHRVTPTTHEHPGDIDVQQRMQKLELGPHSSGGGDASSVVGRGSRRGGGRVLPETISILRTRPEAVTSKKGTSGTPLDLLANYFTVETTPKWGLYQYHVDISPEEDSTGVRKALMRVHSKTLGGYLFDGTVLYTVNRLHPDPMELYSDRKTDNERMRILIKLTCEVSPGDYHYIQIFNIIIRKCFNLLKLQLMGRDYFDPEAKIDIPEFKLQIWPGYKTTINQYEDRLLLVTEIAHKVLRMDTVLQMLSEYAATKGNNYKKIFLEDVVGKIVMTDYNKRTYRVDDVAWNVSPKSTFKMRDENITYIEYYYKKYNLRIQDPGQPLLISRSKPREIRAGLPELIYLVPELCRQTGLSDEMRANFKLMRSLDVHTKIGPDKRIEKLNNFNRRFTSTPEVVEELATWSLKLSKELVKIKGRQLPPENIIQANNVKYPAGDTTEGWTRDMRSKHLLAIAQLNSWVVITPERQRRDTESFIDLIIKTGGGVGFRMRSPDLVVIRHDGPIEYANMCEEVIARKNPALILCVLARNYADRYEAIKKKCTVDRAVPTQVVCARNMSSKSAMSIATKVAIQINCKLGGSPWTVDIPLPSLMVVGYDVCHDTRSKEKSFGAFVATLDKQMTQYYSIVNAHTSGEELSSHMGFNIASAVKKFREKNGTYPARIFIYRDGVGDGQIPYVHSHEVAEIKKKLAEIYAGVEIKLAFIIVSKRINTRIFVQRGRSGENPRPGTVIDDVVTLPERYDFYLVSQNVREGTIAPTSYNVIEDTTGLNPDRIQRLTYKLTHLYFNCSSQVRVPSVCQYAHKLAFLAANSLHNQPHYSLNETLYFL

Description

Endoribonuclease that plays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity (PubMed:19460866, PubMed:27693359). Plays an essential role in meiotic differentiation of spermatocytes, germ cell differentiation and in self-renewal of spermatogonial stem cells (PubMed:19460866, PubMed:25558067, PubMed:27693359). Its presence in oocytes suggests that it may participate in similar functions during oogenesis in females (PubMed:18191035). Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons (PubMed:19460866, PubMed:25558067, PubMed:27693359). Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements (PubMed:19460866, PubMed:25558067, PubMed:27693359). Recognizes piRNAs containing a phosphate at the 5'-end and a 2'-O-methylation modification at the 3'-end (PubMed:27693359). Strongly prefers a uridine in the first position of their guide (g1U preference, also named 1U-bias) and a complementary adenosine in the target (t1A bias) (PubMed:24757166, PubMed:27693359). Plays a key role in the piRNA amplification loop, also named ping-pong amplification cycle: antisense piRNA-bound Siwi and sense piRNA-bound Ago3 reciprocally cleave complementary transcripts, to couple the amplification of piRNAs with the repression of transposable elements. In this process Siwi acts as a 'slicer-competent' piRNA endoribonuclease that cleaves primary piRNAs, which are then loaded onto Ago3 (PubMed:27693359). In this process, Siwi requires the RNA unwinding activity of the RNA helicase Vasa for the release of the cleavage products (PubMed:25558067).

Cofactor

Mg(2+)

Subunit

Interacts (when symmetrically methylated) with Papi/TDRKH (PubMed:23970546, PubMed:26919431). Interacts with Vasa (PubMed:25558067).

Similarity

Belongs to the argonaute family. Piwi subfamily.
Belongs to the argonaute family.

Keywords

3D-structure   Complete proteome   Cytoplasm   Developmental protein   Differentiation   Endonuclease   Hydrolase   Meiosis   Metal-binding   Methylation   Nuclease   Reference proteome   RNA-binding   RNA-mediated gene silencing   Spermatogenesis  

Feature

chain  Piwi-like protein Siwi

Uniprot

A8D8P8   A0A3S2N9X4   A0A2A4J8B1   A0A2H1VJ96   S4PEK0   A0A194R5H6   + More
A0A0K0K804   A0A194PIH2   A0A2H1V8Y3   A0A2H1V5K6   A0A212FEI4   U5IBZ2   A0A067RM87   S4W045   A0A1Y1N8W6   A0A1L7NZM7   A0A2J7RAL3   K4MR11   A0A0T6B2M2   A0A1B6KR98   A0A1B6MJY0   A0A1B6LZB3   A0A0L7RCR7   A0A1B6F8K3   K7ISV0   A0A1W4XLK3   V5I9K0   A0A232ES36   A0A1B6I4E4   A0A088ARC0   A0A2I6PIY2   A0A026WTT9   A0A1B6DVK7   D6WSL8   A0A0J7L598   A0A1B6DT98   A0A1B6CSA2   C9E0T3   C9E0T4   A0A232FMW2   E2AFM7   A0A151II10   A0A195ED42   A0A0P4VWF7   A0A067RW77   A0A151XBT8   K7IP24   A0A0C9RXH0   A0A224X701   A0A023F683   A0A0C9Q8U0   A0A195FXG8   A0A158NV03   A0A195B8E8   A0A069DWG2   A0A146L4W1   A0A0V0G407   A0A1B6CDN8   F4WNM9   E2ALX7   K7J555   A0A1B6DLF2   A0A1L7NZN1   A0A336MED6   A0A336MWF3   A0A232FJQ3   A0A195CQF2   A0A126G917   E0W0U4   A0A1B6GNL7   A0A195EMS4   A0A0N0U5A5   A0A158NCK2   A0A336L7R3   A0A195BXG1   A0A336MSM0   A0A195F956   A0A0A9Z7D5   A0A2S2R8W0   A0A2J7QB22   F4WX64   J9JYB0   Q16IF2   A0A182GB18   Q16ZS3   A0A1S4FZX7   E2BM63   M4QXX0   A0A1S4J618   B0W5T3   B0W5P0   J9JWH1   J9K744   A0A1I8PZG7   A0A1I8PZE8   A0A151X5U6   A0A1Q3FC13   A0A1Q3G0H1   A0A182G925   A0A1S4J6G4  

EC Number

3.1.26.-

Pubmed

18191035    19121390    17698031    19460866    23970546    24757166    + More
25558067    26919431    27693359    23622113    26354079    22118469    24845553    23792802    28004739    28034629    23122849    20075255    28648823    29267401    24508170    30249741    18362917    19820115    20513057    20798317    27129103    25474469    21347285    26334808    26823975    21719571    26868596    20566863    25401762    17510324    26483478   

EMBL

AB332313    AB372006    EU143547    BABH01033344    EU034629    RSAL01000375    + More
RVE42096.1    NWSH01002708    PCG67642.1    ODYU01002684    SOQ40502.1    GAIX01004467    JAA88093.1    KQ460883    KPJ11116.1    JX481277    AGS40930.1    KQ459603    KPI92848.1    ODYU01001293    SOQ37310.1    ODYU01000777    SOQ36079.1    AGBW02008943    OWR52118.1    JX998175    AFW04603.1    KK852427    KDR24113.1    KF032645    AGO85970.1    GEZM01010669    JAV93928.1    LC194148    BAW35370.1    NEVH01006567    PNF37866.1    JQ434103    AFV31611.1    LJIG01016089    KRT81648.1    GEBQ01026026    JAT13951.1    GEBQ01003782    JAT36195.1    GEBQ01010950    JAT29027.1    KQ414615    KOC68594.1    GECZ01023197    GECZ01007473    JAS46572.1    JAS62296.1    AAZX01003322    GALX01002800    JAB65666.1    NNAY01002506    OXU21173.1    GECU01025898    JAS81808.1    MG266017    AUM60044.1    KK107109    QOIP01000008    EZA59378.1    RLU19528.1    GEDC01007582    JAS29716.1    KQ971354    EFA07425.1    LBMM01000695    KMQ97723.1    GEDC01008396    JAS28902.1    GEDC01021000    JAS16298.1    GQ444142    ACV84377.1    GQ444143    ACV84378.1    NNAY01000022    OXU31859.1    GL439118    EFN67778.1    KQ977588    KYN01664.1    KQ979074    KYN22764.1    GDKW01001568    JAI55027.1    KDR24114.1    KQ982316    KYQ57823.1    GBYB01012591    JAG82358.1    GFTR01008201    JAW08225.1    GBBI01001987    JAC16725.1    GBYB01010683    JAG80450.1    KQ981215    KYN44539.1    ADTU01002871    ADTU01002872    KQ976565    KYM80517.1    GBGD01000526    JAC88363.1    GDHC01016829    JAQ01800.1    GECL01003336    JAP02788.1    GEDC01025741    JAS11557.1    GL888237    EGI64222.1    GL440682    EFN65569.1    AAZX01015855    GEDC01010784    JAS26514.1    LC194149    BAW35371.1    UFQT01001059    SSX28665.1    UFQT01003030    SSX34460.1    NNAY01000117    OXU30813.1    KQ977408    KYN02941.1    KR012681    ALC79956.1    DS235862    EEB19250.1    GECZ01031744    GECZ01005733    JAS38025.1    JAS64036.1    KQ978691    KYN29202.1    KQ435791    KOX74374.1    ADTU01011951    UFQS01002193    UFQT01002193    SSX13459.1    SSX32890.1    KQ976396    KYM92985.1    UFQT01002105    SSX32695.1    KQ981727    KYN36973.1    GBHO01004331    GBHO01004330    GBRD01000007    GDHC01012215    GDHC01005575    GDHC01000298    JAG39273.1    JAG39274.1    JAG65814.1    JAQ06414.1    JAQ13054.1    JAQ18331.1    GGMS01017205    MBY86408.1    NEVH01016302    PNF25798.1    GL888417    EGI61307.1    ABLF02030598    CH478081    EAT34046.1    JXUM01157421    JXUM01157422    KQ572743    KXJ68003.1    CH477485    EAT40148.1    GL449158    EFN83189.1    KC316041    AGH30330.1    DS231844    EDS35736.1    EDS35693.1    ABLF02019072    ABLF02019073    ABLF02066598    ABLF02038523    ABLF02038526    KQ982490    KYQ55785.1    GFDL01009929    JAV25116.1    GFDL01001779    JAV33266.1    JXUM01153240    KQ571658    KXJ68137.1   

Proteomes

UP000005204    UP000283053    UP000218220    UP000053240    UP000053268    UP000007151    + More
UP000027135    UP000235965    UP000053825    UP000002358    UP000192223    UP000215335    UP000005203    UP000053097    UP000279307    UP000007266    UP000036403    UP000000311    UP000078542    UP000078492    UP000075809    UP000078541    UP000005205    UP000078540    UP000007755    UP000009046    UP000053105    UP000007819    UP000008820    UP000069940    UP000249989    UP000008237    UP000002320    UP000095300   

PRIDE

A8D8P8   

Pfam

PF02171   Piwi        + More
PF02170   PAZ
PF16486   ArgoN
PF08699   ArgoL1
PF17906   HTH_48

Interpro

IPR012337   RNaseH-like_sf        + More
IPR003100   PAZ_dom       
IPR003165   Piwi       
IPR036085   PAZ_dom_sf       
IPR036397   RNaseH_sf       
IPR032474   Argonaute_N       
IPR014811   ArgoL1       
IPR041426   Mos1_HTH       
IPR011989   ARM-like       
IPR021133   HEAT_type_2       
IPR016024   ARM-type_fold       

SUPFAM

SSF53098   SSF53098        + More
SSF101690   SSF101690       
SSF48371   SSF48371       

Gene 3D

3.30.420.10     1.25.10.10    

ProteinModelPortal

A8D8P8   A0A3S2N9X4   A0A2A4J8B1   A0A2H1VJ96   S4PEK0   A0A194R5H6   + More
A0A0K0K804   A0A194PIH2   A0A2H1V8Y3   A0A2H1V5K6   A0A212FEI4   U5IBZ2   A0A067RM87   S4W045   A0A1Y1N8W6   A0A1L7NZM7   A0A2J7RAL3   K4MR11   A0A0T6B2M2   A0A1B6KR98   A0A1B6MJY0   A0A1B6LZB3   A0A0L7RCR7   A0A1B6F8K3   K7ISV0   A0A1W4XLK3   V5I9K0   A0A232ES36   A0A1B6I4E4   A0A088ARC0   A0A2I6PIY2   A0A026WTT9   A0A1B6DVK7   D6WSL8   A0A0J7L598   A0A1B6DT98   A0A1B6CSA2   C9E0T3   C9E0T4   A0A232FMW2   E2AFM7   A0A151II10   A0A195ED42   A0A0P4VWF7   A0A067RW77   A0A151XBT8   K7IP24   A0A0C9RXH0   A0A224X701   A0A023F683   A0A0C9Q8U0   A0A195FXG8   A0A158NV03   A0A195B8E8   A0A069DWG2   A0A146L4W1   A0A0V0G407   A0A1B6CDN8   F4WNM9   E2ALX7   K7J555   A0A1B6DLF2   A0A1L7NZN1   A0A336MED6   A0A336MWF3   A0A232FJQ3   A0A195CQF2   A0A126G917   E0W0U4   A0A1B6GNL7   A0A195EMS4   A0A0N0U5A5   A0A158NCK2   A0A336L7R3   A0A195BXG1   A0A336MSM0   A0A195F956   A0A0A9Z7D5   A0A2S2R8W0   A0A2J7QB22   F4WX64   J9JYB0   Q16IF2   A0A182GB18   Q16ZS3   A0A1S4FZX7   E2BM63   M4QXX0   A0A1S4J618   B0W5T3   B0W5P0   J9JWH1   J9K744   A0A1I8PZG7   A0A1I8PZE8   A0A151X5U6   A0A1Q3FC13   A0A1Q3G0H1   A0A182G925   A0A1S4J6G4  

PDB

5GUH     E-value=0,     Score=4340

KEGG

100125336    K02156 aubergine | (RefSeq) Aubergine, Piwi, SIWI, siwi1; aubergine protein

PATHWAY

04320    Dorso-ventral axis formation - Bombyx mori (domestic silkworm)

GO

GO:0030154   GO:0031047   GO:0007283   GO:0000287   GO:0034584   GO:0051321   GO:0034587   GO:0004521   GO:1990923   GO:0005737   GO:0007275   GO:0003676   GO:0003743   GO:0005515   GO:0005886   GO:0007155   GO:0007156   GO:0008237   GO:0043401   GO:0003707   GO:0051537  

Subcellular location

Cytoplasm   Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis (PubMed:25558067).   With evidence from 9 publications.

Length:

899

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.03302

Exp number, first 60 AAs:

0

Total prob of N-in:

0.00098

outside

1  -  899

 

 

Pi

21.023039

Theta

20.67662

Tajima's D

-0.89538

CLR

0.874361

CSRT

0.157242137893105

Interpretation

Uncertain