Gene

KWMTBOMO07194  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA010385

Annotation

PREDICTED:_non-lysosomal_glucosylceramidase-like_isoform_X2_[Bombyx_mori]

Location

Bomo_Chr12(-):7665982-7670066      View in EpiBrowser

Full name

Non-lysosomal glucosylceramidase      

Location in the cell

Mitochondrial   Reliability : 2.108

CDS

ATGGATATCGAACCCGCAGCCAAAGAACACCGCGATTTTGAAATGGACAAACCATCGAAATTCGGCTTAAAACTTCCCTTAGATCATGAATTTCCCCTGAAGAGCAAGCAGATTATAATTCCAAGACCAAAACAAATACTGGACATGATACCGCTTTCAGTCAGGTATTTCAAGTATTGTTTGAGCAAAAAACTGCGTAAAAGGCGTCCAATAATGGATTATTTCCAAATGATTTCAGCACAGCGAATGTACGGATGTCCCATTGGTGGAATTGGAGGTGGAACCATAGGGAGGGGTTTTAAAGGGGAATTTTGTCGTTTTCAACTACATCCTGGTATATATGAATATGTCACTGTCCCAGAATGCCAGTTTATTGTAAACATAAGGAATGCTAACAATGAGACAATATTCCAATCAGTTCTTTCCACCTACAGCAAACCAAAGAAAGCCCCGGCATCTTGGGAATGGAACCTTGATGGTGCAAAATGTGAATACACAGCACTGTATCCGAGGGCATGGACAACATATGATCTGTCAAAATATGGTGTGAAACTTGTGTGTAGACAAATATCACCAGTAATACCTCATAACTATAAGGACAGCAAGACGTGA

Protein

MDIEPAAKEHRDFEMDKPSKFGLKLPLDHEFPLKSKQIIIPRPKQILDMIPLSVRYFKYCLSKKLRKRRPIMDYFQMISAQRMYGCPIGGIGGGTIGRGFKGEFCRFQLHPGIYEYVTVPECQFIVNIRNANNETIFQSVLSTYSKPKKAPASWEWNLDGAKCEYTALYPRAWTTYDLSKYGVKLVCRQISPVIPHNYKDSKT

Description

Non-lysosomal glucosylceramidase that catalyzes the hydrolysis of glucosylceramide (GlcCer) to free glucose and ceramide.
Non-lysosomal glucosylceramidase that catalyzes the conversion of glucosylceramide to free glucose and ceramide.

Catalytic Activity

a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-acylsphing-4-enine + D-glucose

Similarity

Belongs to the non-lysosomal glucosylceramidase family.

Keywords

Cell membrane   Complete proteome   Glycoprotein   Glycosidase   Hydrolase   Lipid metabolism   Membrane   Phosphoprotein   Reference proteome   Sphingolipid metabolism   Transmembrane   Transmembrane helix  

Feature

chain  Non-lysosomal glucosylceramidase

Uniprot

H9JLI4   A0A2A4JS75   A0A194PHH7   A0A194R067   A0A3S2MAG2   A0A212FL48   + More
A0A1J1J8E5   A0A1Q3EX63   A0A2M4AD48   A0A182G2J8   D6WP98   A0A182VG64   A0A182XDH2   A0A1B0D2U1   A0A182F4Y7   A0A2M4CTR6   A0A1B0CJL7   A0A1Y1M7I9   A0A1S4EYW6   A0A1Y1M6Q4   A0A336LV92   A0A0N7ZAY4   A0A182J903   A0A1I8Q3I3   A0A1L8E2K8   A0A1L8E229   A0A232FNF0   A0A182Y986   A0A336KNI8   A0A2S2NZ18   A0A1I8MB60   A0A2S2NJ98   A0A1A9X643   K7J4S3   A0A0A1X3L1   A0A0Q9W6A9   W8BBN7   A0A1I8Q3F9   A0A0L0BZ66   A0A084VYE4   A0A2C9GPH9   A0A1Y1M358   A0A1S4GZD2   A0A2H8TUQ7   A0A1I8MB61   A0A1B0BIX2   A0A0A1WH86   A0A0Q9XEM2   A0A1W4W6S7   A0A0K8VHU5   A0A0R1DLK3   A0A1B0AJL2   A0A034VAV9   A0A182Q9Z3   T1JDY2   Q7PWW0   A0A0K8U0T0   A0A1W4UXX5   A0A034VCF4   Q17L47   A0A0R3NV91   A0A3B0JQ10   W8BYU9   A0A0Q5WAH1   X1WIY6   B0WC06   E0VQ36   A0A0P8Y3U4   N6TMK2   X2JAF7   A0A0J9TN83   A0A182VU42   A0A2S2QCY2   B4MZB3   A0A1A9VQD2   A0A1B6DQC9   B4P0J9   A0A1B6DSU6   A0A1B6CFK6   A0A1Y1M3J2   A0A1B6GW49   A0A1W4UJ88   B5DJY3   A0A182RUT0   A0A0Q5WJC6   A0A3B0JNL6   B3N9T4   X2J9U5   A0A0J9R344   Q7KT91   B4Q5I4   B4HXF9   A0A0J9R2C1   A0A182MMM5   B3MKK0   A0A2T7NF00   A0A1B6LPM5  

EC Number

3.2.1.45

Pubmed

19121390    26354079    22118469    26483478    18362917    19820115    + More
28004739    17510324    28648823    25244985    25315136    20075255    25830018    17994087    24495485    26108605    24438588    12364791    17550304    25348373    15632085    20566863    23537049    10731132    12537568    12537572    12537573    12537574    16110336    17569856    17569867    22936249    12537569    18327897   

EMBL

BABH01005693    BABH01005694    BABH01005695    BABH01005696    NWSH01000781    PCG74252.1    + More
KQ459603    KPI92767.1    KQ460883    KPJ11193.1    RSAL01000003    RVE54749.1    AGBW02007871    OWR54456.1    CVRI01000074    CRL08234.1    GFDL01015179    JAV19866.1    GGFK01005378    MBW38699.1    JXUM01040103    JXUM01040104    JXUM01040105    JXUM01040106    KQ561232    KXJ79285.1    KQ971354    EFA07388.1    AJVK01002842    AJVK01002843    AJVK01002844    GGFL01004568    MBW68746.1    AJWK01014724    AJWK01014725    AJWK01014726    AJWK01014727    GEZM01041578    JAV80275.1    GEZM01041586    JAV80270.1    UFQT01000222    SSX21962.1    GDRN01092272    GDRN01092271    JAI60136.1    GFDF01001349    JAV12735.1    GFDF01001350    JAV12734.1    NNAY01000010    OXU32049.1    UFQS01000634    UFQT01000634    SSX05588.1    SSX25947.1    GGMR01009796    MBY22415.1    GGMR01004635    MBY17254.1    AAZX01005602    GBXI01008373    JAD05919.1    CH940654    KRF77831.1    GAMC01012127    JAB94428.1    JRES01001125    KNC25310.1    ATLV01018361    ATLV01018362    ATLV01018363    KE525231    KFB42988.1    APCN01003341    APCN01003342    GEZM01041579    JAV80274.1    AAAB01008984    GFXV01005103    MBW16908.1    JXJN01015223    GBXI01016236    JAC98055.1    CH933807    KRG02958.1    GDHF01013840    JAI38474.1    CM000157    KRJ98122.1    GAKP01019748    GAKP01019747    GAKP01019745    GAKP01019744    JAC39207.1    AXCN02000732    JH432114    EAA14848.4    GDHF01031985    JAI20329.1    GAKP01019749    GAKP01019746    JAC39203.1    CH477219    EAT47379.1    CH379061    KRT04860.1    OUUW01000006    SPP82482.1    GAMC01012126    JAB94429.1    CH954177    KQS70445.1    ABLF02018149    ABLF02018157    ABLF02018163    ABLF02059614    DS231884    EDS43066.1    DS235389    EEB15492.1    CH902620    KPU73469.1    APGK01029406    APGK01029407    APGK01029408    APGK01029409    KB740734    ENN79258.1    AE014134    AHN54442.1    CM002910    KMY90235.1    GGMS01006401    MBY75604.1    CH963913    EDW77386.1    GEDC01009404    JAS27894.1    EDW88993.1    GEDC01008565    JAS28733.1    GEDC01025021    JAS12277.1    GEZM01041580    JAV80273.1    GECZ01003088    JAS66681.1    EDY70607.2    KQS70446.1    SPP82483.1    EDV58579.1    AHN54441.1    KMY90234.1    BT024218    BT044085    AY058463    CM000361    EDX05028.1    CH480818    EDW51739.1    KMY90233.1    AXCM01000045    EDV31553.2    PZQS01000013    PVD19748.1    GEBQ01014339    GEBQ01001449    JAT25638.1    JAT38528.1   

Proteomes

UP000005204    UP000218220    UP000053268    UP000053240    UP000283053    UP000007151    + More
UP000183832    UP000069940    UP000249989    UP000007266    UP000075903    UP000076407    UP000092462    UP000069272    UP000092461    UP000075880    UP000095300    UP000215335    UP000076408    UP000095301    UP000092443    UP000002358    UP000008792    UP000037069    UP000030765    UP000075840    UP000092460    UP000009192    UP000192223    UP000002282    UP000092445    UP000075886    UP000007062    UP000192221    UP000008820    UP000001819    UP000268350    UP000008711    UP000007819    UP000002320    UP000009046    UP000007801    UP000019118    UP000000803    UP000075920    UP000007798    UP000078200    UP000075900    UP000000304    UP000001292    UP000075883    UP000245119   

PRIDE

H9JLI4    X2JAF7    X2J9U5    Q7KT91   

Pfam

PF12215   Glyco_hydr_116N        + More
PF04685   DUF608
PF07500   TFIIS_M
PF05422   SIN1
PF16979   SIN1_PH
PF01096   TFIIS_C
PF08711   Med26
PF16978   CRIM
PF10609   ParA

Interpro

IPR024462   GH116_N        + More
IPR012341   6hp_glycosidase-like_sf       
IPR008928   6-hairpin_glycosidase_sf       
IPR006775   GH116_catalytic       
IPR014551   B_Glucosidase_GBA2-typ       
IPR003617   TFIIS/CRSP70_N_sub       
IPR006289   TFSII       
IPR031567   CRIM_dom       
IPR032679   Sin1_N       
IPR017923   TFIIS_N       
IPR001222   Znf_TFIIS       
IPR003618   TFIIS_cen_dom       
IPR011993   PH-like_dom_sf       
IPR036575   TFIIS_cen_dom_sf       
IPR035441   TFIIS/LEDGF_dom_sf       
IPR031313   Sin1_PH_dom       
IPR019591   Mrp/NBP35_ATP-bd       
IPR027417   P-loop_NTPase       
IPR033756   YlxH/NBP35       
IPR000808   Mrp_CS       

SUPFAM

SSF48208   SSF48208        + More
SSF46942   SSF46942       
SSF47676   SSF47676       
SSF52540   SSF52540       

Gene 3D

1.50.10.10     1.10.472.30     1.20.930.10     2.30.29.30    

CDD

cd02037  MRP-like

ProteinModelPortal

H9JLI4   A0A2A4JS75   A0A194PHH7   A0A194R067   A0A3S2MAG2   A0A212FL48   + More
A0A1J1J8E5   A0A1Q3EX63   A0A2M4AD48   A0A182G2J8   D6WP98   A0A182VG64   A0A182XDH2   A0A1B0D2U1   A0A182F4Y7   A0A2M4CTR6   A0A1B0CJL7   A0A1Y1M7I9   A0A1S4EYW6   A0A1Y1M6Q4   A0A336LV92   A0A0N7ZAY4   A0A182J903   A0A1I8Q3I3   A0A1L8E2K8   A0A1L8E229   A0A232FNF0   A0A182Y986   A0A336KNI8   A0A2S2NZ18   A0A1I8MB60   A0A2S2NJ98   A0A1A9X643   K7J4S3   A0A0A1X3L1   A0A0Q9W6A9   W8BBN7   A0A1I8Q3F9   A0A0L0BZ66   A0A084VYE4   A0A2C9GPH9   A0A1Y1M358   A0A1S4GZD2   A0A2H8TUQ7   A0A1I8MB61   A0A1B0BIX2   A0A0A1WH86   A0A0Q9XEM2   A0A1W4W6S7   A0A0K8VHU5   A0A0R1DLK3   A0A1B0AJL2   A0A034VAV9   A0A182Q9Z3   T1JDY2   Q7PWW0   A0A0K8U0T0   A0A1W4UXX5   A0A034VCF4   Q17L47   A0A0R3NV91   A0A3B0JQ10   W8BYU9   A0A0Q5WAH1   X1WIY6   B0WC06   E0VQ36   A0A0P8Y3U4   N6TMK2   X2JAF7   A0A0J9TN83   A0A182VU42   A0A2S2QCY2   B4MZB3   A0A1A9VQD2   A0A1B6DQC9   B4P0J9   A0A1B6DSU6   A0A1B6CFK6   A0A1Y1M3J2   A0A1B6GW49   A0A1W4UJ88   B5DJY3   A0A182RUT0   A0A0Q5WJC6   A0A3B0JNL6   B3N9T4   X2J9U5   A0A0J9R344   Q7KT91   B4Q5I4   B4HXF9   A0A0J9R2C1   A0A182MMM5   B3MKK0   A0A2T7NF00   A0A1B6LPM5  

PDB

5OST     E-value=8.69048e-10,     Score=149

KEGG

101743464    K17108 non-lysosomal glucosylceramidase [EC:3.2.1.45] | (RefSeq) non-lysosomal glucosylceramidase-li

PATHWAY

00511    Other glycan degradation - Bombyx mori (domestic silkworm)
00600    Sphingolipid metabolism - Bombyx mori (domestic silkworm)
01100    Metabolic pathways - Bombyx mori (domestic silkworm)

GO

GO:0004553   GO:0006680   GO:0016020   GO:0004348   GO:0006629   GO:0016021   GO:0016139   GO:0008422   GO:0008270   GO:0006351   GO:0003676   GO:0005634   GO:0006355   GO:0008206   GO:0046527   GO:0005886   GO:0005524  

Subcellular location

Nucleus  
Cell membrane  

Length:

203

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.951

Exp number, first 60 AAs:

0.01038

Total prob of N-in:

0.12224

outside

1  -  203

 

 

Pi

15.774678

Theta

14.593288

Tajima's D

-0.911977

CLR

3.114168

CSRT

0.156442177891105

Interpretation

Uncertain